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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 20, Issue 6 - Dec 2005
Volume 20, Issue 5 - Oct 2005
Volume 20, Issue 4 - Aug 2005
Volume 20, Issue 3 - Jun 2005
Volume 20, Issue 2 - Apr 2005
Volume 20, Issue 1 - Feb 2005
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The Use of Stem Cells as Medical Therapy
Son Eun-Hwa ; Pyo Suhkneung ;
KSBB Journal, volume 20, issue 1, 2005, Pages 1~11
Recently, there has been extremely active in the research of stem cell biology. Stem cells have excellent potential for being the ultimate source of transplantable cells for many different tissues. Researchers hope to use stem cells to repair or replace diseased or damaged organs, leading to new treatments for human disorders that are currently incurable, including diabetes, spinal cord injury and brain diseases. There are primary sources of stem cells like embryonic stem cells and adult stem cells. Stem cells from embryos were known to give rise to every type of cell. However, embryonic stem cells still have a lot of disadvantages. First, transplanted cells sometimes grow into tumors. Second, the human embryonic stem cells that are available for research would be rejected by a patient's immune system. Tissue-matched transplants could be made by either creating a bank of stem cells from more human embryos, or by cloning a patient's DNA into existing stem cells to customize them. However, this is laborious and ethically contentious. These problems could be overcome by using adult stem cells, taken from a patient, that are treated to remove problems and then put back. Nevertheless, some researchers do not convince that adult stem cells could, like embryonic ones, make every tissue type. Human stem cell research holds enormous potential for contributing to our understanding of fundamental human biology. In this review, we discuss the recent progress in stem cell research and the future therapeutic applications.
Separation of Volatile Compounds from Tuna Fish Oil With Supercritical Carbon Dioxide
Roh Hyung Seob ; Youn Hyun Seok ; Jung Sun Mi ; Hong Yeon Ryun ; Kang Kil Yoon ; Chun Byung Soo ;
KSBB Journal, volume 20, issue 1, 2005, Pages 12~17
Despite a wide range of fatty acids in fish oil, its the usage are very limited owing to off-flavors and volatile compounds in the oil. A way to extract and remove volatile compounds was performed at a semi-flow extractor by using supercritical carbon dioxide
. Samples of the oil were treated at the conditions which ranged from
and from 80 to 200 bar with 10 mL/min flow rate of carbon dioxide. In the oil the volatile compounds were analyzed by gas chromatography. Before extraction with
the oil sample was detected over 129 peaks but 99 compounds were identified. The results demonstrated that at
and 200 bar extraction condition the volatile compounds in the tuna fish oil were removed, except for 14 compounds identified after extraction and other
extraction conditions reached to high reduction of the volatile compounds.
Biodegradation of PPC-PVL and PVL by Stenotrophomonas maltophilia
Park Suk Kyoung ; Ju Hyun ; Cho Sung Ki ; Kim Donguk ; Oh Kwang Joong ; Ree Moonhor ;
KSBB Journal, volume 20, issue 1, 2005, Pages 18~20
Polypropylene carbonate-polyvalerolactone(PPC-PVL) and polyvalerolactone (PVL) produced from exhausted carbon dioxide were degraded by Stenotrophomonas maltophilia separated from soils of waste landfill. The biodegradation was confirmed by FTIR spectrum. PPC-PVL and PVL were degraded
respectively, at 28 days of pure Stenotrophomonas maltophilia culture
Paraquat Induced Heme Oxygenase-1 in Dopaminergic Cells
Chun Hong Sung ;
KSBB Journal, volume 20, issue 1, 2005, Pages 21~25
Paraquat, a widely used herbicide, has been suggested as a potential risk factor for Parkinson's disease. Heme oxygenase-1(HO-1), a marker for oxidative stress and endoplasmic reticulum(ER) stress, is known to catalyze heme to biliverdin, carbon monoxide and free iron in response to various stimuli. Here we show that paraquat activates HO-1 expression in a time-and dose-dependent manner in substantia nigra(SN) dopaminergic neuronal cells. Activation of Ho-1 by paraquat was regulated primarily at the level of gene transcription. Deletion analysis of the promoter and the 5' distal enhancers, E1 and E2, of the HO-1 gene revealed that the E2 enhancer is a potent inducer of the paraquat-dependent Ho-1 gene expression in dopamninergic neuronal cells. Mutational analysis of the E2 enhacer further demonstrated that the transcription factor activator protein-1(AP-1) plays an important role in mediating paraquat-induced HO-1 gene transcription. Moreover, using specific inhibitors of the mitogen-activated protein kinases(MAPKs), we investigated the role of paraquat and MAPKs for HO-1 gene regulation in dopaminergic cells. The c-Jun N-terminal kinase(JNK) inhibitor SP600125 significantly suppressed the expression of HO-1 by paraquat. All these results demonstrate that induction of HO-1 by paraquat requies the activation of the AP-1 and JNK pathway.
Effects of Carbon Substrates on Exopolysaccharide Production by Enterobacter sp.
Lee Ju-Ha ; Lee Shin-Young ;
KSBB Journal, volume 20, issue 1, 2005, Pages 26~33
The effects of carbon sources for exopolysaccharide production during batch cultivation of an Enterobacter sp. isolated from the composter were investigated. The highest amount of exopolysaccharide was obtained when lactose was used as carbon source. Lactose in medium was converted into glucose and galactose. Glucose was metabolized fast and was completely consumed, but about
of lactose was accumulated as galactose. On the other hand, enzyme activity was about
unit with the increase of lactose concentration. Thus, it was considered that the exopolysaccharide might be produced in the course of that lactose was hydrolyzed into glucose and galactose by
with respect to that enzyme activity on lactose hydrolysis was accorded to the exopolysaccharide production. When glucose and galactose were added to lactose medium, respectively, it could be considered that glucose was as a repressor and galactose was as a inducer for
synthesis even though the mechanisms were not elucidated. The increase of lactose concentration was almost ineffective to the specific growth rate
but showed the difference in the biomass content. The higher carbon source concentration, the more residual sugar remained. It was assumed that the optimum lactose concentration for exopolysaccharide production was
On the other hand, it was considered that the nitrogen acted as growth limiting nutrients to the cell growth. In the cases of 30 and 70 g/L of the fixed carbon concentrations, the increase of the nitrogen sources concentration caused a remarkable increase within the range of
of the specific growth rate, respectively, while there was no significant difference in biomass.
Optimization of Culture Conditions for the [+]-Eudesmin Production in Magnolia Sieboldii Cells
Hwang Sung Jin ;
KSBB Journal, volume 20, issue 1, 2005, Pages 34~39
In order to product the furofuranoid lignans, (+)-eudesmin which is one of the secondary products from Magnolia sieboldii. through cell suspension cultures; various culture media, initial sucrose concentration, elicitations, shaking speeds, and inoculum sizes. Among the culture media tested, MS medium had a pronounced effect on suspension cell growth and (+)-eudesmin contents. The maximum dry cell weight (DCW) of 3.71 g per flask was obtained at inoculum size of 0.5 g and in MS medium supplemented with
sucrose plus 0.5 mg/L 2,4-D after 8 weeks. (+)-Eudesmin biosynthesis was stimulated with high initial sucrose concentration ,and the maximum (+)-eudesmin production of
DCW was achieved at 200mg/L chitosan and
initial medium sucrose. The optimal shaking speeds for dry biomass accumulation and (+)-eudesmin contents was 130 rpm. This work is considered to be helpful for large-scale bioprocessing of Magnolia sieboldii suspension cell cultures in bioreactor.
Effects of the Spatholobi calulis extract on Antioxidation and Inhibition of Matrix Metalloproteinase in Human Skin Fibroblasts
Sim Gwan Sub ; Kim Jin Hwa ; Lee Dong Hwan ; Park Sung Min ; Pyo Hyeong Bae ; Zhang Yong He ; Lee Bum Chun ;
KSBB Journal, volume 20, issue 1, 2005, Pages 40~45
The production of matrix metalloproteinases (MMPs) by the UV irradiated skin fibroblast and the degradation of exracellular matrix (ECM) by these enzymes is known as one of the main reasons of photoaging. In this study, to investigate the relationship between aging and Spatholobi caulis extract, we examined the effects of antioxidant, in vitro MMP inhibition and expression of UVA-induced MMP-1 in human dermal fibroblasts. Spathoiobi caulis extract was found to show scavenging activities of radicals and reactive oxygen species (ROS) with the
against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and
against superoxide radicals in the xanthine/xanthine oxidase system, respectively. Spatholobi caulis extract inhibited the activities of MMP-1 in a does-dependent manner and the IC50 value calculated from semi-log plots was
. Also, UVA induced MMP expression was reduced
by treatment with Spatholobi caulis extract, and MMP-1 mRNA expression was reduced in a dose-dependent manner. Therefore Spatholobi caulis extract was able to significantly inhibit MMP expression in protein and mRNA level. All these results suggested that Spatholobi caulis extract may act as an anti-aging agent by antioxidation and reducing UVA-induced MMP-1 production.
pH-Sensitive Release of Indomethacin from Curdlan Acetate Microspheres
Lee Chang-Moon ; Lee Young-Jin ; Kim Hyung-Ju ; Park Hee-Jung ; Lee Ki-Young ;
KSBB Journal, volume 20, issue 1, 2005, Pages 46~49
Curdlan, a natural
has been studied as drug carrier due to its unique properties including its thermal gelling characters. In this study, curdlan was chemically acetylated for pH-sensitive drug delivery. Curdlan acetate microspheres(CAMs) were prepared by the solvent evaporation method. The size of the CAMs was below
The drug loading efficiency of microspheres was approximately
. In the swelling test, the CAMs showed pH-sensitive behavior. The swelling capacity of microspheres at pH 7.4 was much greater than at pH 1.4. Also, release rate of indomethacin(IND) at pH 7.4 from the CAMs was faster than that at pH 1.4. Therefore the CAMs have potential for the controlled release of IND over an extended period of time.
Preparation of Carbon Nanomaterial from the Microbial Cellulose
Kim Bong Gyun ; Sohng Jae Kyung ; Liou Kwangkyoung ; Lee Hei Chan ;
KSBB Journal, volume 20, issue 1, 2005, Pages 50~54
Tar is often produced during the carbonization of cellulose that limits the formation fibrous structure of the carbonized sample. This problem was reduced by applying a high temperature
during carbonization process. Alternatively, dry cellulose was immersed in toluene and ultrasonicated prior to carbonization. In both cases, complete fibrous structures were not achieved. The formation of tar was reduced by the heat treatment of cellulose in the presence of HCI vapor before carbonization process. Such treatment before carbonization yielded mostly the fibrous structures of the carbonized sample as evident from SEM analysis. Similar results were found when the cellulose was subjected to a heat treatment in an inert condition followed by the removal of tar by the oxidation process prior to the carbonization.
Toxicity Test of Biodiesel and Biodiesel-derived Neopentyl Polyol Ester Lubricant Oil Base Using Microalgae
Jung Haeyoung ; Lee Jung-Heon ; Yoo Jeong Woo ; Kim Eui Yong ; Chae Hee Jeong ;
KSBB Journal, volume 20, issue 1, 2005, Pages 55~59
Toxicity test for biodiesel (BD), biodiesel-derived neopentyl polyol ester (NPE) lubricant oil base, lubricant oil for diesel engine (LODE) and petroleum diesel (PD) was carried out using microalgae, Chlorella vulgaris. According to the method of OECD 201, the
values of BD and NPE were estimated as 84 and 69 mg/L, respectively, which indicates that these compounds are classified as slightly toxic compounds. The
values of LODE and PD were measured as 42 and 24 mg/mL, respectively, showing that these compounds are considered as moderately toxic compounds.
Characteristics of Submerged and Solid-State Fermentations for Production of Arachidonic Acid Mortierella alpina
Shin Hyung Tai ; Lee Soo Won ; Park Ki Moon ; Song Jae Whan ; Suh Dong Sang ; Lee Jae Heung ;
KSBB Journal, volume 20, issue 1, 2005, Pages 60~65
The objective of this work was to evaluate a solid-state fermentation process for the practical production of arachidonic acid(AA) by Mortierella alpina ATCC 32222. In the present investigation, batch culture kinetics for both submerged- and solid-state fermentations was carried out at
to identify the relationship between growth and arachidonic acid (AA) production. Glucose and yeast extract were used in submerged fermentations by using flasks, while rice bran was used as a sole raw material in the other type of fermentations by using a series of Petri dishes. It was evident that a mixed-growth associated pattern existed between the two variables, irrespective of modes of fermentations. The effect of carbon to nitrogen (CfN) ratio on AA production in solid-state fermentation was studied in the range of 6.5 - 20. As a result, an optimum condition was found to be 6.5. Supplementary carbon source was not necessary to meet the optimum C/N ratio. Unlike the Previous results obtained by other researchers, a supplement of sodium glutamate up to
(w/w) to the rice bran medium did not have a positive effect on the AA productivity. However, an increase in AA productivity was obtained with the rice bran medium supplemented with sesame oil.
Optimization of Pigment Production of Monascus Purpureus P-57 in Liquid Culture
Park Chi Duck ; Jung Hyuck Jun ; Yu Tae Shick ;
KSBB Journal, volume 20, issue 1, 2005, Pages 66~70
Optimization of culture conditions for pigment production of Monascus purpureus P-57 mutant was investigated in liquid culture. The optimum composition of medium for the pigment production was
, the optimum initial pH was 5.0. And the optimum culture conditions was at
for 8 days under 150 rpm with shaking. M. purpureus P-57 mutant produced the highest pigment as 356.04 units at red pigment and as 268.20 units at yellow pigment, and produced high cell mass as 15.00 g/L in liquid culture under the optimum conditions.