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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 20, Issue 6 - Dec 2005
Volume 20, Issue 5 - Oct 2005
Volume 20, Issue 4 - Aug 2005
Volume 20, Issue 3 - Jun 2005
Volume 20, Issue 2 - Apr 2005
Volume 20, Issue 1 - Feb 2005
Selecting the target year
Microbial hydrogen production: Dark Anaerobic Fermentation and Photo-biological Process
Kim, Mi-Sun ; Baek, Jin-Sook ;
KSBB Journal, volume 20, issue 6, 2005, Pages 393~400
) as a clean, and renewable energy carrier will be served an important role in the future energy economy. Several biological
production processes are known and currently under development, ranging from direct bio-photolysis of water by green algae, indirect bio-photolysis by cyanobacteria including the separated two stage photolysis using the combination of green algae and photosynthetic microorganisms or green algae alone, dark anaerobic fermentation by fermentative bacteria, photo-fermentation by purple bacteria, and water gas shift reaction by photosynthetic or fermentative bacteria. In this paper, biological
production processes, that are being explored in fundamental and applied research, are reviewed.
Optimization of Hydrogen Production using Clostridium beijerinckii KCTC 1785
Kim, Jung-Kon ; Nhat, Le ; Kim, Seong-Jun ; Kim, Si-Wouk ;
KSBB Journal, volume 20, issue 6, 2005, Pages 401~407
Optimum culture conditions and medium composition for hydrogen production by Clostridium beijerinckii KCTC 1785 were investigated. Initial pH and temperature for growth were 7.0 and
, respectively. Agitation accelerated the hydrogen production. Although C. beijerinckii KCTC 1785 could grow up to 6%(w/v) glucose in the medium, the optimum glucose concentration for hydrogen production was 4% and hydrogen content in the biogas was 37%(v/v). However, the economical glucose concentration for hydrogen production was 1% regarding to the residual glucose which was not used in the medium. During hydrogen fermentation, acetic and butyric acid were produced simultaneously. High concentrations of acetic(>5,000 mg/L) or butyric(>3,000 mg/L) acid inhibited hydrogen production. When pH was maintained at 5.5 in the batch fermentation, 1,728 mL of hydrogen was produced from 0.5% glucose within 15 hr.
yield was estimated to be 1.23 mol
glucose. It was found that yeast extract or tryptose in the medium was essential for hydrogen production.
Partial Pressures of
and Fate of By-products in Anaerobic Bio-Hydrogen Fermentation
Park, Woo-Shin ; Kim, In-S. ;
KSBB Journal, volume 20, issue 6, 2005, Pages 408~412
In a previous research, it has been found that it could be possible to increase the partial pressure of hydrogen and hydrogen yield by scavenging the
from the heads pace of reactor. In this research, the positive and negative effects of the
scavenging especially on the fate of by-products were investigated by a batch experiment. Production and conversion of by-products had critical relationships with hydrogen evolution and consumption. The maximum hydrogen fraction in the headspace was increased from 66.4 to 91.2% by removing the
in the headspace and the degradation rate of glucose was also enhanced. The removal of
effectively hindered the homoacetogenesis but caused several negative phenomena. The degradation of ethanol, one of the main products, was inhibited by the high partial pressure of hydrogen and/or the absence of
. Also it was observed that other by-products such as propionate, propanol, acetone, etc. could not be degraded further after produced from glucose. On the other hand, solventogenesis was not observed in spite of the high hydrogen partial pressure apart from previous researches and it might hinder the excess production of acetate, which could cause overall inhibition. From this research, it could be implicated that the
scavenging method could be recommended if the fermentation was purposed to produce hydrogen and ethanol.
Hydrogen Production by Purple Sulfur Bacteria, Thiocapsa roseopersicina in Photoheterotrophic Culture Condition
Kim, Mi-Sun ; In, Sun-Kyoung ; Baek, Jin-Sook ; Lee, Jeong-K. ;
KSBB Journal, volume 20, issue 6, 2005, Pages 413~417
The purple sulfur phototrophic bacterium, Thiocapsa roseopersicina NCIB 8347 has been studied on hydrogen production and cell growth under different culture conditions, such as light source, light intensity, and growth temperature. T. roseopersicina showed maximum cell growth of 1.38 and 1.42 g-DCW/L under 7.5-10 klux of halogen and fluorescent light, respectively, and produced maximum amount of hydrogen with values of 0.90 and 0.48
-DCW under the irradiation of 10 klux of halogen and fluorescent light, respectively. The optimum growth temperature for hydrogen production was
, and hydrogen production rate was lowered over
. When T. roseopersicina was grown photoheterotrophically under irradiation of 8-9 klux of halogen lamp, the generation time was 4.2 hr. The strains started producing hydrgen from the middle of the logarithmic growth phase and continued until succinate concentration leveled out.
Improvement of Photoheterotrophic
production of Rhodobacter sphaeroides by Removing Ammonium Ion Effect Exerted on Nitrogenase
Jin, Sang-Hoon ; Kim, Mi-Sun ; Lee, Jeong-Kug ;
KSBB Journal, volume 20, issue 6, 2005, Pages 418~424
Photoheterotrophic evolution of molecular hydrogen by Rhodobacter sphaeroides is mediated by nitrogenase that is regulated transcriptionally and post-translationally by ammonium ion. Two PII-like proteins, GlnB and GlnK, play key roles in mediating inhibition and repression of nitrogenase in the presence of ammonium ion. glnB and glnK of R. sphaeroides were interrupted to abolish the ammonium ion effect controlling nitrogenase. Ammonium ion effect was still observed in mutant having an interruption in either glnB or glnK. However, the nitrogenase activity of glnB-glnK double mutant is not affected by ammonium ion.
evolution was improved by increasing gene dosages of nitrogenase-coding genes, nifHDK in trans in glnB-glnK double mutant.
Thermophilic Biohydrogen Production from Glucose with a Long-term Operation of CSTR
Ahn, Yeong-Hee ; Oh, You-Kwan ; Park, Sung-Hoon ;
KSBB Journal, volume 20, issue 6, 2005, Pages 425~430
was produced for 1 year using a bench-scale continuous stirred tank reactor(CSTR). The CSTR was inoculated with anaerobically digested sludge after heat treatment and fed with a glucose-based medium. The reactor showed relatively short start-up period(30 days) and high maximal
glucose). Keeping pH 5.0 or less suppressed methanogenic activity. Bacteria affiliated with Thermoanaerobacterium thermosaccharolyticum kept being dominant from approximately 40 days as determined by DGGE. Environmental perturbation(pH or temperature) caused the decrease of biomass concentration in the reactor and the instability of reactor performance,
production rate and
yield. The unstable performance was accompanied with high concentration of lactate in the effluent. Taken together, the poor recovery of CSTR after perturbations could be partly explained by low biomass concentration and/or metabolic shift of the major population in the CSTR.
Molecular Analysis of the Microorganisms in a Thermophilic CSTR used for Continuous Biohydrogen Production
Oh, You-Kwan ; Park, Sung-Hoon ; Ahn, Yeong-Hee ;
KSBB Journal, volume 20, issue 6, 2005, Pages 431~437
Molecular methods were employed to investigate microorganisms in a thermophilic continuous stirred tank reactor(CSTR) used for continuous
production. The reactor was inoculated with heat-treated anaerobic sludge and fed with a glucose-based medium. Denaturing gradient gel electrophoresis showed dynamic changes of bacterial populations in the reactor during 43 days of operation. Gas composition was constant from approximately 14 days but population shift still occurred. Populations affiliated with Fervidobactrium gondwanens and Thermoanaerobacterium thermosaccharolyticum were dominant on 21 and 41 days, respectively. Keeping pH of the medium at 5.0 could suppress methanogenic activity that was detected during initial operation period.
and mcrA detected in the samples obtained from the reactor or inoculum suggested the heat treatment condition employed in this study is not enough to remove methanogens in the inoculum. PCR using primer sets specific to 4 main orders of methanogens suggested that major
-consuming methanogens in the CSTR belong to the order Methanobacteriales.
Continuous Bio-hydrogen Production from Food Waste and Waste Activated Sludge
Kim, Dong-Kun ; Lee, Yun-Jie ; Kim, Dong-Im ; Kim, Ji-Seong ; Yu, Myong-Jin ; Pak, Dae-Won ; Kim, Mi-Sun ; Sang, Byoung-In ;
KSBB Journal, volume 20, issue 6, 2005, Pages 438~442
Batch experiments were performed to investigate the effects of volumetric mixing ratio(v/v) of two substrates, food wastes(FW) and waste activated sludge(WAS). In batch experiments, optimum mixing ratio for hydrogen production was found at
v/v % addition of WAS. CSTR(Continuous Stirred tank reactor) was operated to investigate the hydrogen productivity and the microbial community under various HRTs and volumetric mixing ratio(v/v) of two substrates. The maximum yield of specific hydrogen production, 140 mL/g VSS, was found at HRT of 2 day and the volumetric mixing ratio of 20:80(WAS:FW). The spatial distribution of hydrogen producing bacteria was observed in anaerobic fermentative reactor using fluorescent in situ hybridization(FISH) method.
Effect of Hydraulic Retention Time on Fermentative Hydrogen and Byproducts Production from Food Waste
Kim, Sang-Hyoun ; Shin, Hang-Sik ;
KSBB Journal, volume 20, issue 6, 2005, Pages 443~446
Hydrogen fermentation from food waste was attempted at different hydraulic retention time(HRT, 18-42 h). A continuous reactor fed with ground, alkali-treated and diluted food waste(average VS 4.4%) exhibited stable hydrogen production during 126 days. Hydrogen production depended on HRT, resulting in the maximum values of 25.8 mL
, 0.36 mol
and 0.91 L
at HRT 30 h. n-Butyrate and isopropanol production increased with hydrogen production increased, while acetate production decreased. The fermentation efficiency ranged from 53.3 to 65.7%, which implied that hydrogen fermentation would substitute conventional acidogenesis of food waste.
Hydrogen Production from Fruit Wastes by Immobilized Cells of Enterobacter cloacae VJ-1
Lee, Ki-Seok ; Huh, Yang-Il ; Chung, Seon-Yong ; Kang, Chang-Min ;
KSBB Journal, volume 20, issue 6, 2005, Pages 447~452
The hydrogen production using immobilized cellsl was conducted using fruit wastewaters at various culture conditions. Three kinds of fruit wastewaters, melon, watermelon and pear were used. Sodium alginate was used as immobilization material. Among them, concentration of reducing sugar which was one of the main components in fruit was the highest at watermelon wastewater, and also hydrogen production was the highest as 2319.2 mL/L in it. Although hydrogen production was not much changed according to sodium alginate concentration, its production was the most at 3%(w/v). As bead size as small, hydrogen production was higher. With inspection of interior, it confirmed that the cell grew well in bead. But the addition of amino acids using as agent for metabolite production had almost no affected on hydrogen productivity. The effective range of
addition on hydrogen production were up to 1.2 g/L, and above the concentration, it inhibited the productivity. Organic acids produced during watermelon fermentation were mainly lactic acid, butyric acid, abd acetic acid; and a little of propionic acid.
Sulfate Modulation for Hydrogen Production by Chlamydomonas reinhardtii in Continuous Culture
Kim, Jun-Pyo ; Park, Tai-Hyun ; Kim, Mi-Sun ; Sim, Sang-Jun ;
KSBB Journal, volume 20, issue 6, 2005, Pages 453~457
We investigated the effect of sulfate re-addition on hydrogen production under sulfur-deprived condition. When the final concentration of sulfate to cell suspensions(
) was increased, chlorophyll concentration, culture density, and total amount of
produced, increased up to an optimal concentration of
. Maximum hydrogen volume was 236 mL
. However, the addition of excess sulfate(above
) delayed the start of hydrogen production and the induction of hydrogenase. Accordingly, the final yield of hydrogen production was reduced. Using these results, we attempted the continuous and sustained hydrogen production by sulfate re-addition(
) using a single C. reinhardtii culture for up to 4 cycles. In total, hydrogen production volume was 625 mL
Continuous Hydrogen Production by Heterotrophic Growth of Citrobacter amalonaticus Y19 in Trickle Bed Reactor
Park, Ji-Young ; Lee, Tae-Ho ; Oh, You-Kwan ; Kim, Jun-Rae ; Seol, Eun-Hee ; Jung, Gyoo-Yeol ; Kim, Mi-Sun ; Park, Sung-Hoon ;
KSBB Journal, volume 20, issue 6, 2005, Pages 458~463
] from CO and water was continuously produced in a trickle bed reactor(TBR) using Citrobacter amalonaticus Y19. When the strain C. was cultivated in a stirred-tank reactor under a chemoheterotrophic and aerobic condition, the high final cell concentration of 13 g/L was obtained at 10 hr. When the culture was switched to an anaerobic condition with the continuous supply of gaseous CO, CO-dependent hydrogenase was fully induced and its hydrogen production activity approached 16 mmol/g cell/hr in 60 hr. The fully induced C. amalonaticus Y19 cells were circulated through a TBR packed with polyurethane foam, and the TBR was operated for more than 20 days for
production. As gas retention time decreased or inlet CO partial pressure increased,
production rate increased but the conversion from CO to
decreased. The maximum
production rate obtained was 16 mmol/L/hr at the gas retention time of 25 min and the CO inlet partial pressure of 0.4 atm. The high
production rate was attributed to the high cell density in the liquid phase circulating the TBR as well as the high surface area of polyurethane foam used as packing material of the TBR.
pH-dependent Metabolic Flux Shift in Novel Hydrogen-Producing Bacterium Enterobacter sp. SNU-1453
Shin, Jong-Hwan ; Yoon, Jong-Hyun ; Ahn, Eun-Kyoung ; Sim, Sang-Jun ; Kim, Mi-Sun ; Park, Tai-Hyun ;
KSBB Journal, volume 20, issue 6, 2005, Pages 464~469
For the biological production of hydrogen, a new fermentative hydrogen-producing bacterium, Enterobacter sp. SNU-1453, was isolated from a domestic landfill. During the culture of this bacterium, pH significantly decreased with the accumulation of various organic acids, and consequently this inhibited the production of hydrogen. It was found that the metabolic flux in this bacterium depended on the pH and affected the hydrogen production. A butanediol pathway was dominant during the fermentation when pH was not controlled. By controlling the pH at 7 this pathway can be shifted to a mixed acid pathway, which is favorable to the production of hydrogen.