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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 21, Issue 6 - Dec 2006
Volume 21, Issue 5 - Oct 2006
Volume 21, Issue 4 - Aug 2006
Volume 21, Issue 3 - Jun 2006
Volume 21, Issue 2 - Apr 2006
Volume 21, Issue 1 - Feb 2006
Selecting the target year
Paclitaxel : Recovery and Purification in Commercialization Step
Kim Jin-Hyun ;
KSBB Journal, volume 21, issue 1, 2006, Pages 1~10
The recovery and purification of a paclitaxel from plant cell cultures is essential to commercial process. This review describes a large-scale recovery and purification method for producing paclitaxel, to guarantee high purity and yield from plant cell cultures. Also, the process of separation and purification is optimized in conjunction with a extraction step, pre-purification, purification, and polishing (drying) as an integrated process to meet final product quality requirements such as purity, residual solvents, product morphologies, impurities, bacterial endotoxin, etc. This information is very useful for production and quality control of pharmaceuticals in commercialization step.
A Study on the Extraction and Efficacy of Bioactive Compound from Hovenia dulcis
Kim Sung-Mun ; Kang Sung-Hee ; Ma Jin-Yeul ; Kim Jin-Hyun ;
KSBB Journal, volume 21, issue 1, 2006, Pages 11~15
The biological activities of extracts from the fruit, stem, and leaf of Hovenia dulcis were examined. In the batch mode of operation, the fruit, stem, and leaf of Hovenia dulcis were extracted with hot water for 10 hr. The fruit extract of Hovenia dulcis gave the highest activity for decreasing alcohol concentration which was 138% of control. The equilibrium between bioactive compound in the fruit (size : 4 mm) and hot water solution was reached within 6 hr and the recovery was 95% by three-times extraction. The fruit extract of Hovenia dulcis showed significant alcohol decrease in blood and hepatoprotective activity against
-toxicity in rat. The fruit extract significantly inhibited the elevation of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) levels.
Two-dimensional Electrophoretic Analysis of Nucleotide phosphate Kinase Mediated Hydrogen Peroxide Cross-linking in Saccharamyces cerevisiae
Moon Hae-Jeong ; Yun Dae-Jin ; Park Chang-Ho ;
KSBB Journal, volume 21, issue 1, 2006, Pages 16~19
Oxidative modification of nucleoside diphosphate kinase (NDPK) is identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometer. The quaternary structure of NDPK appears to be regulated by cross-linking with an oxidant,
. We compared roles of NDPK in each of wild type and ynk mutant against oxidative stress. Six specific proteins changed by
were identified using two-dimensional electrophoretic analysis. YNK regulated several proteins, related to
signaling functions. These results suggest that one of the important functions of NDPK is the regulation of cellular redox state.
Fibrinolytic Activities and Effects of Gamma-Irradiated on Seeds from Coix lacryma-jobi L. Carthamus tinctorius L. and Malva verticillata L.
Kwon Su-Jung ; Lim Chae-Young ; Kim Jae-Sung ; Park Min-Hee ; Lee Sook-Young ;
KSBB Journal, volume 21, issue 1, 2006, Pages 20~27
The fibrinolytic activities of soluble proteins extracted from seeds of Coix lacryma-jobi L., Carthamus tinctorius L. and Malva venicillata L. were studied. Fibrinolytic activity of extract from C. lacryma-jobi L. showed 1.3 times higher than plasmin used as positive control. The fibrinolytic enzyme was confirmed and extracted directly from seed of C. lacryma-jobi L. by a fibrin zymography. The protein was composed of a single polypeptide and its apparent molecular weight was found to be 7.8 kDa, as judged by SDS-polyacrylamide gel electrophoresis. The effect of temperature for the proteolytic enzyme activity were stabilized above
and then dramatically decreased. Also, the enzyme activity was clearly inhibited by APMSF, PMSF and TPCK, suggesting that it is a member of the chymotrypsin-like serine pretense. In addition, effects of gamma-irradiated on seed of each plants were revealed that 8 Gy and 64 Gy were higher than others. This result shown that gamma-irradiation of seeds were capable to increase the fibrinolytic activity. All these results suggest the pretense is a fibrinolytic enzyme belong to a family of chymotrypsin-like serine pretense.
Horizontal Flow Zeolite-Filled Reed Bed with Intermittent Feeding for Sewage Treatment
Seo Jeoung-Yoon ; Kim Ean-Ho ;
KSBB Journal, volume 21, issue 1, 2006, Pages 28~33
A sewage was treated using a horizontal flow zeolite-filled reed bed. The sewage from the student dormitory of Changwon National University was fed into the reed bed for 10 minutes every 6 hours at the hydraulic load of
day. The filtering height of the reed bed was 100 cm and the zeolite mixture was filled in the reed bed. The mixture consisted of the same volume of two types of zeolite :
in diameter. Annual average removal efficiency was SS
and T-P 42.8%. T-N of effluent was mostly
and the concentration of
in effluent was lower than 0.1 mg/L. All removal efficiencies did not show a remarkable seasonal change.
Selection of Biofilter Support for Removing MEK
Jeong Gwi-Taek ; Lee Gwang-Yeon ; Lee Kyoung-Min ; Sunwoo Chang-Shin ; Lee Woo-Tae ; Jung Seong-Ho ; Cha Jin-Myoung ; Jang Young-Seon ; Park Don-Hee ;
KSBB Journal, volume 21, issue 1, 2006, Pages 34~41
The aim of this study is the development of biological removal process of methyl ethyl ketone (MEK) in odor gas, which is generated from the waste food recycling process. To develop the removal process of odor gas, MEK, the selection of proper biofilter support was carried out. When the biofilter equipment was passed by synthetic odor gas composed of 250 ppm of MEK, the maximum removal was achieved to
for polypropylene fibril as support. Under the same experimental conditions, the maximum removal in polyurethane support was obtained to
. Finally, the maximum removal in volcanic stone support was
Selection and Antifungal Activity of Antagonistic Bacterium Bacillus subtilis KMU-13 against Cucumber scab, Cladosporium cucumerinum KACC 40576
Park Sung-Min ; Lee Jun-Seuk ; Park Chi-Duck ; Lee Jung-Hun ; Jung Hyuck-Jun ; Yu Tae-Shick ;
KSBB Journal, volume 21, issue 1, 2006, Pages 42~48
Bacillus subtilis KMU-13 was isolated from the Lillehammer forest soils at Norway and shown a strong antifungal activity on cucumber scab, Cladosporium cucumerinum KACC 40576. B. subtilis KMU-13 produced a maximum level of antifungal substance under incubation aerobically at
, 180 rpm for 48 hours in LB broth containing 0.5% maltose and 0.5% bactopeptone and initial pH adjusted to 6.0. Butanol extract of cultured broth was confirmed inhibitory zone by plate assay and Rf 0.64 value substance by thin layer chromatography (TLC) represented high antifungal activity against C. cucumerinum KACC 40576 and also shown fungal growth inhibitory activity against Botytis cinerea KACC 40573, C. gloeosporioides KACC 40804, D. byoniae KACC 40669, F. oxysporum KACC 40037, F. oxysporum KACC 40052, F. oxysporum f. sp. radicis-lycopersici KACC 40537, F. oxysporum KACC 40902, M. cannonballus KACC 40940, P. cambivora KACC 40160, R. soiani AG-1 KACC 40101, R. solani AG-4 KACC 40142, and S. scleotiorum KACC by agar diffusion method.
Determination of Biological kinetic Parameters for Pharmaceutical Wastewater
Lee Young-Rak ; Choi Kwang-Keun ; Lee Jin-Won ;
KSBB Journal, volume 21, issue 1, 2006, Pages 49~53
The aim of this research is to estimate the values of biological kinetic parameters of pharmaceutical wastewater for understanding biochemical properties. Maximum specific growth rate (
), yield coefficient (Y), and half-velocity coefficient (KS) were determined using oxygen uptake rate (OUR), and the results were 10.49/day (0.437/hr), 0.655, and 38.89 mg/L, respectively. Measured
by nonlinear regression of Monod equation was 10.63/day (or 0.443/hr), and this value was similar with above result. These parameters may be used to increase efficiency of pharmaceutical wastewater treatment and to determine amount of oxygen needed to removal BOD and dissolved oxygen in activated sludge process.
Characterization of PVA Degrading Enzymes from Microbacterium barkeri KCCM 10507 and Paenibacillus amylolyticus KCCM 10508
Choi Kwang-Keun ; Kim Sang-Yong ; Lyoo Won-Seok ; Lee Jin-Won ;
KSBB Journal, volume 21, issue 1, 2006, Pages 54~58
The purpose of this study is to search the characteristics of PVA degrading enzymes which were obtained from Microbacterium barkeri KCCM 10507 and Paenibacillus amylolyticus KCCM 10508, respectively. As a result of the PVA degrading test using crude enzymes, the activity of SAO (secondary alcohol oxidase) was maximized after 2 or 3 days from start of the test, while the activity of BDH (
-diketone hydrolase) was gradually increased during the test. Activities of them were maintained in the presence of PVA, but as PVA was gradually degraded, their activity was decreased. PVA was inoculated again into the media, their activity was revealed. This result indicated that above two different enzymes were closely connected with PVA degradation and PVA was degraded by activity of SAO and BDH. Maximum activity of them was 1.5-1.8 unit for SAO and 1.5-2.0 unit for BDH under
and pH 7.8-8.8, respectively.
Chemometric Analysis of 2D Fluorescence Spectra for Monitoring and Modeling of Fermentation Processes
Kang Tae-Hyoung ; Sohn Ok-Jae ; Kim Chun-Kwang ; Chung Sang-Wook ; Rhee Jong-Il ;
KSBB Journal, volume 21, issue 1, 2006, Pages 59~67
2D spectrofluorometer produces many spectral data during fermentation processes. The fluorescence spectra are analyzed using chemometric methods such as principal component analysis (PCA), principal component regression (PCR) and partial least square regression (PLS). Analysis of the spectral data by PCA results in scores and loadings that are visualized in score-loading plots and used to monitor a few fermentation processes by S. cerevisae and recombinant E. coli. Two chemometric models were established to analyze the correlation between fluorescence spectra and process variables using PCR and PLS, and PLS was found to show slightly better calibration and prediction performance than PCR.
Pretreatment of Feedstock by Ion Exchange Resin Catalyst in Biodiesel process
Lee Soo-Gon ; Chae Hee-Jeong ; Yoo Jeong-Woo ; Kim Eui-Yong ;
KSBB Journal, volume 21, issue 1, 2006, Pages 68~71
Free fatty acids are not esterified by alkaline catalyst transesterification. They are detrimental to the quality specifications in biodiesel. Therefore, we tried to find solid catalyst to remove free fatty acids in feedstock. Amberlyst 15 resin was selected as the best catalyst, and the moisture content containing in the resin was found to be important for the reaction. The removal efficiency of free fatty acids was gradually decreased from 97% to 70% by ten times reuse of resin. In the transesterificaion reaction by KOH catalyst, soap formation could be decreased by 58.3% using the feedstock pretreated by resin. Consequently, the purity of biodiesel was enhanced about 10%, as compared with the non-treated feedstock.
Comparison of Antibacterial and Antioxidant Activities of Mushroom Mycelium Culture Extracts Cultivated in the Citrus Extracts
Kim Man-Chul ; Kim Min-Joo ; Kim Taeg ; Park Guen-Tae ; Son Hong-Joo ; Kim Gi-Young ; Choi Woo-Bong ; Oh Duck-Chul ; Heo Moon-Soo ;
KSBB Journal, volume 21, issue 1, 2006, Pages 72~78
This study was carried out to investigate the antimicrobial and antioxidative effects of mycelium cultural extract from mushroom. Mushroom mycelium was grown in a defined synthetic liquid medium and citrus extracts, and the culture extracts were examined for antioxidant and antibacterial activity. Myceliums of Phellinus linteus, Cordyceps militaris, Coriolus versicolor, Sparassic crispa, Agaricus blazei, lnonotus obliquus, Lentinus edodes, Hericium erinacium, Gonoderma lucidium in 10% citrus extract supplemented medium and synthesis medium were incubated in a shaking incubator (120 rpm,
days. The antimicrobial activity of the culture fluid of mushroom mycelium grown in submerged liquid culture was tested against 12 microorganisms which were fish pathogens and common bacterial species. The culture extracts showed high activity against Vibrio sp. and had poor effect on Streptocouus sp., S. parauberis, S. iniae. The culture extracts obtained from the synthetic medium showed
of the 1,1-diphenyl-2-picrylhydrazyl radical scavenger activity, the culture extracts obtained from the citrus extracts medium exhibited antioxidant activity up to 55%.