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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 21, Issue 6 - Dec 2006
Volume 21, Issue 5 - Oct 2006
Volume 21, Issue 4 - Aug 2006
Volume 21, Issue 3 - Jun 2006
Volume 21, Issue 2 - Apr 2006
Volume 21, Issue 1 - Feb 2006
Selecting the target year
Microfluidic Immuno-Sensor Chip using Electrical Detection System
Maeng, Joon-Ho ; Lee, Byung-Chul ; Cho, Chul-Ho ; Ko, Yong-Jun ; Ahn, Yoo-Min ; Cho, Nahm-Gyoo ; Lee, Seoung-Hwan ; Hwang, Seung-Yong ;
KSBB Journal, volume 21, issue 5, 2006, Pages 325~330
This study presents the characterization of an integrated portable microfluidic electrical detection system for fast and low volume immunoassay using polystyrene microbead, which are used as immobilization surfaces. In our chip, a filtration method using the microbead was adopted for sample immobilization and immunogold silver staining(IGSS) was used to increase the electrical signal. The chip is composed of an inexpensive and biocompatible Polydimethylsiloxane(PDMS) layer and Pyrex glass substrate. Platinum microelectrodes for electric signal detection were fabricated on the substrate and microchannel and pillar-type microfilters were formed in the PDMS layer. With a fabricated chip, we reacted antigen and antibody according to the procedures. Then, silver enhancer was injected to increase the size of nanogold particles tagged with the second antibody. As a result, microbeads were connected to each other and formed an electrical bridge between microelectrodes. Resistance measured through the electrodes showed a difference of two orders of magnitude between specific and nonspecific immuno-reactions. The detection limit was 10 ng/ml. The developed immunoassay chip reduced the total analysis time from 3 hours to 50 min. Fast and low-volume biochemical analysis has been successfully achieved with the developed microfilter and immuno-sensor chip, which is integrated to the microfluidic system.
Production of Immunostimulating Polysaccharide in Angelica gigas Nakai SusDension Cell Cultures
Kim, Young-Hwa ; Kim, Ik-Hwan ; Kim, Dong-Il ;
KSBB Journal, volume 21, issue 5, 2006, Pages 331~335
Suspension cells of Angelica gigas Nakai were cultivated to produce extracellular polysaccharide(ECP) as immunostimulating agents. Effects of environmental conditions such as sucrose and 2,4-dichlorophenoxyacetic acid(2,4-D) concentrations on the growth and production of ECP were studied using suspension cultures of A. gigas Nakai. Final dry cell weight was increased with an increase of initial sucrose concentration from 30 to 60 g/L. The maximum production of ECP(1.2 g/L) was achieved at an initial sucrose concentration of 50 g/L on day 8. High 2,4-D concentration was effective for ECP production but not for cell growth. In addition, various fungal elicitors were investigated for the enhanced production of ECP in A. gigas suspension cultures. Among the tested fungal elicitors, Verticillium dahliae was the most effective for the production of ECP in A. gigas suspension culture.
Production of Extracellular Polysaccharide by Perfusion Culture of Angelica gigas Nakai Suspension Cells
Kim, Young-Hwa ; Kim, Ik-Hwan ; Kim, Dong-Il ;
KSBB Journal, volume 21, issue 5, 2006, Pages 336~340
High-density perfusion cultivation was performed to produce extracellular polysaccharide(ECP) as immunostimulating agents in suspension cell cultures of Angelica gigas Nakai. In batch culture, the maximum cell density was 16.8 gDCW/L at day 6 and 0.9 g/L of ECP was obtained at day 8. When the medium exchange was started at the fifth day after inoculation for the perfusion culture, high concentration of the cells at 23.8 gDCW/L could be achieved with continuous production of ECP. Treatments of ultrasound and Pluronic F-68 were found to be helpful for the secretion of intracellular ECP into the culture medium.
The Early Detection of the Gram Negative Bacteria using Signification and Fluorescent Dye in the Field
Ha, Yeon-Chul ; Choi, Ki-Bong ; Choi, Jung-Do ;
KSBB Journal, volume 21, issue 5, 2006, Pages 341~346
This study was carried out to establish the optimum condition for cell disruption with a sonificator in the detection of the gram negative bacteria, E. coli for the purpose of developing automatic fluorometer. The efficiency of sonification on the E. coli disruption was greatly dependent on the diameter of sonificator probe tip. The larger sonificator probe diameter showed greater disruption effect. Sonificator probe of 13 mm diameter was the most efficient one for E. coli when sonificated for 20 seconds. The efficiency of the E. coli disruption differed greatly according to the depth of sonificator probe tip sank in the sample solution. The shorter the distance between probe tip end and the bottom of the container, the higher the disruption efficiency. The detection limit of E. coli was
when sample was sonificated for 20 seconds with a sonificator probe of 13 mm diameter.
The Early Detection of the Gram Positive Bacteria using Sonification and Fluorescent Dye in the Field
Ha, Yeon-Chul ; Choi, Ki-Bong ; Choi, Jung-Do ;
KSBB Journal, volume 21, issue 5, 2006, Pages 347~352
This study was carried out to establish the optimum condition for cell disruption with a sonificator in the detection of the gram positive bacteria, Bacillus globigii and Streptococcus epidermidis for the purpose of developing automatic fluorometer. The efficiency of sonificator on the Bacillus globigii and Streptococcus epidermidis disruption differed greatly according to the diameter of sonificator probe tip. The larger sonificator probe diameter showed greater disruption. Bacillus globigii was more disruptive than Streptococcus epidermidis. Sonificator probe of the 13 mm diameter was the most efficient one when sample was sonificated for 20 seconds. The detection limits of Bacillus globigii and Streptococcus epidermidis were
respectively when samples were sonificated for 20 seconds with a sonificator probe of 13 mm diameter.
Characteristics of Biological Hydrogen Production from Sewage Sludge treated by Optimal Solubilization Technology
Choi, Kwang-Keun ; Kim, Sun-Jip ; Lee, Tae-Joon ; Park, Dae-Won ; Lee, Won-Kwon ;
KSBB Journal, volume 21, issue 5, 2006, Pages 353~359
The purpose of this study is to search the optimal solubilization technology which could be applied to sewage sludge, and is to find the characteristics of biological hydrogen production when the sludge treated by optimal method was used as a sole substrate. As a result of the test, treatment technology mixed with alkali and ultrasonic treatment was very powerful tool for treating sewage sludge with high solubilization, and its ratio and elution rate of organic material was 0.9 and 0.076
, respectively. When the sludge treated by above optimal technology was used, 4.4 ml
VSS of hydrogen was produced. Finally, When the sludge treated by above optimal technology was used, 13.4 ml
VSS of hydrogen was produced under optimum pH.
Surface Modification of Nano Porous Silica Particle for Enzyme Immobilization
Cho, Hyung-Min ; Kim, Jong-Kil ; Kim, Ho-Kun ; Lee, Eun-Kyu ;
KSBB Journal, volume 21, issue 5, 2006, Pages 360~365
The objectives of this study were to develop nano-pore silica particles and to modify its surface for use as an enzyme immobilization matrix. Sol-gel reaction was used to produce silica particles of various nano pore sizes with hydroxyl groups on their surfaces. The surface was modified with aldehyde that was confirmed by fluorescence imaging. Trypsin was covalently immobilized by reductive amination. Surface density of the immobilized trypsin was ca.
, which was approximately 17- and 35-fold higher than those from the surfaces with hydroxyl and amine group, respectively. About 90% of the initial enzyme activity was maintained after the 12th use of repeated use. When compared with the commercial matrices, the nano-pore silica particle was superior in terms of immobilization yield and specific activity. This study suggests the nano porous silica particles can be used as enzyme immobilization matrix for industrial applications.
Effect of Nitrogen Source on the Change of UDP-glucose and
Park, Yang-Ho ; Lee, Jung-Heon ;
KSBB Journal, volume 21, issue 5, 2006, Pages 366~370
In this research, analysis of UDP-glucose a precursor of
-1,3-glucan by high performance liquid chromatography(HPLC) was established using a reversed phase system. One of key metabolite UDP-glucose was selected and its concentration changes was measured with the change of fermentation conditions. The effects of fermentation conditions with/without nitrogen source for cell growth on
-1,3-glucan production were dependent on the UDP-glucose concentration. The UDP-glucose was synthesized rapidly during cell exponential growth period and maintained high during
-1,3-glucan production period. The UDP-glucose concentration was higher for
-1.3-glucan production fermentor than that for cell growth fermentor. The
-1,3-glucan production was optimal at pH 5.5 and synthesis of
-1,3-glucan was greatest at pH 5.5.
Determination of Loxoprofen Adsorption Isotherms by Frontal Analysis and Pulse Input Method
Lee, Eun ; Park, Joon-Sub ; Kim, In-Ho ;
KSBB Journal, volume 21, issue 5, 2006, Pages 371~375
Frontal analysis(FA) and Pulsed input method(PIM) have been frequently utilized to measure isotherm of single solute, as well as non-competitive isotherms of two solutes in chromatography(1). FA and PIM were used in this study as complementary methods to measure adsorption isotherms of loxoprofen racemate in HPLC. Prior to FA and PIM experiments, measurements of loxoprofen solubility were made at hexane/ethanol=50/50, 80/20, 95/5(v/v) with acetic acid(0.5%) for adjusting pH. The last composition(95/5) of hexane/ethanol allows us to separate loxoprofen racemate into two forms(retentate, extract). PIM and FA were used to determine the isotherms of re-and ex-loxoprofen.
The Functional Effects of Fermented Pine Needle Extract
Park, Ga-Young ; Li, Hongxian ; Hwang, In-Deok ; Cheong, Hyeon-Sook ;
KSBB Journal, volume 21, issue 5, 2006, Pages 376~383
Pine needle(Pinus densiflora sieb, et zucc) extract has been used to improve cardiovascular disorders, detoxification of nicotine, the infirmities of age and curing diseases of unidentified symptoms. It has various useful components including amino acids, vitamin C, terpenoids and chlorophyll. In this study we have identified 8 different yeast strains that are developed spontaneously causing self fermentation in the extract. The self-fermented pine extract(SFPE) inhibited the growth of some bacterial strains like E. coli, Bacillus subtilis and Staphylococcus aureus. The SFPE(
) showed 90% NBT superoxide scavenging activities which is similar for all tested samples of different ages. The 7 years old SFPE(0.15 mg/ml and 0.3 mg/ml) caused relaxation of spontaneous contraction and relaxation rhythm of thoracic arterial tissues from rat. Therefore, SFPE has useful effects such as antibacterial, antioxidant and improved blood circulation and could be a good source of functional food development.
The Production of Lincomycin by Repeated Batch Cultures of Immobilized Streptomyces lincolnensis
Kim, Chang-Joon ; Chun, Gie-Taek ; Chang, Yong-Keun ; Kim, Sung-Bae ;
KSBB Journal, volume 21, issue 5, 2006, Pages 384~388
The production stability of high-yielding mutants of Streptomyces lincolnensis immobilized on celite beads was examined in repeated batch cultures. We also explored the feasibility of immobilization of vegetative mycelial cells on pre-wetted celite beads, which is practical method for cell immobilization. Repeated transfer of immobilized cells into fresh medium every 10 days increased productivity of immobilized cells and maximum concentration of lincomycin, 1007
mg/L, was obtained at the end of the ninth cycle. A 1.4-fold higher productivity was obtained in immobilized-cell culture than that obtained by suspended-cell culture. When pre-wetted beads were inoculated with vegetative mycelia and cultured a slightly higher amount of immobilized cells and lincomycin was obtained more than those obtained by culture of spores immobilized on dry beads. This result indicates that immobilization of mycelial cells on pre-wetted beads was readily available. This technique is simple and no additional facilities are required for cell immobilization.
-agarase in Batch and Fed-batch Culture by Agarivorans sp. JA-1
Lee, Song-Ae ; Kim, Jin-Uk ; Jung, Jong-Geun ; Kim, In-Hae ; Lee, Sang-Hyeon ; Kim, Sang-Jin ; Lee, Jae-Hwa ;
KSBB Journal, volume 21, issue 5, 2006, Pages 389~393
-agarase production of Agarivorans sp, JA-1 isolated from north-eastern sea of Jeju marine environment was studied. Optimal cell growth was definite that the medium containing agar is 0.2%. The decreasing pattern of viscosity and agar concentration was same and they reached almost zero after 15 hours. Fed-batch culture was studied to improve agarase productivity by Agarivorans sp. JA-1 in marine broth containing 2.0 g/L agar with intermittent addition of 0.8 g agar two times. The hydrolysis products were identified oligosaccharide of degrees of polymerization 6.
The DeveloDment of PaDain which is Extremely Stable to Negative Ionic Environment by Directed Molecular Evolution
Kang, Whan-Koo ; Hwang, Sun-Duk ; Kim, Hyoung-Sik ; Jeung, Jong-Sik ; Lee, Bheong-Uk ;
KSBB Journal, volume 21, issue 5, 2006, Pages 394~400
When the papain, which is a sort of Cystein protease, is applied to the outer skin, it decomposes the protein which forms the peeled outer skin and speeds up metabolism. Therefore, it is one of the most important cosmetics compositic which keeps the function of skin normal. When the papain is used in cosmetics with surfactant, the activity of papain is reduced rapidly. In this study, the modified papain with extreme stability negative ionic environment was developed by directed evolution
Retention Mechanism of Caffeine and Tryptophan in Macroporous Poly[Methacrylic Acid-co-Ethylene Glycol Dimenthacrylate] Rods
Jin, Longmei ; Yan, Hongyuan ; Zheng, Jinzhu ; Row, Kyung-Ho ;
KSBB Journal, volume 21, issue 5, 2006, Pages 401~404
Macroporous Poly(Methacrylic acid-co-Ethylene Glycol Dimethacrylate) Rods were in situ thermal initialized within a empty column(
) by free radical polymerization. The polymerization mixture was consisted of monomer, cross-linking monomer, porogenic solvent, initiator and control the ratio of these materials, column efficiency could be developed. Caffeine and tryptophan as separation substances and the retention mechanism of this kind of monolithic column was mainly hydrogen bond function.