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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 21, Issue 6 - Dec 2006
Volume 21, Issue 5 - Oct 2006
Volume 21, Issue 4 - Aug 2006
Volume 21, Issue 3 - Jun 2006
Volume 21, Issue 2 - Apr 2006
Volume 21, Issue 1 - Feb 2006
Selecting the target year
A Research and Application of Active Ingredients in Bokbunja (Rubus coresnus Miuuel)
Kwon, K.H. ; Cha, W.S. ; Kim, D.C. ; Shin, H.J. ;
KSBB Journal, volume 21, issue 6, 2006, Pages 405~409
Bokbunja (Rubus coreanus Miquel) is a wild berry belonging to Rosaceae genus of which application areas have been expanded due to many health effects. Bokbunja berry contains carbohydrates, protein, fats and dietary fibers as major components and various flavonoids such as tannins, volatile components including organic acids, alcohols and hydrocarbons as minor ones. Those active compounds attribute to the following health effects: increase in immune function and antioxidant activity, rise in hormone secretion, suppression of the growth of type B hepatitis virus, and control of weight gain. To utilize the above activities, many products have been developed by using Bokbunja berry extracts in the area of foods, liquors, and cosmetics. Recently, pure compounds have been isolated from the extracts for the development of medicines. In this mini review, the state-of-the-art of Bokbunja researches and applications including papers and patents is summarized.
Comparison of Volatile Flavor Compounds in Bokbunja [Rubus coreanus Miquel] Wines With and Without Mushroom Extracts
Shin, H.J. ; Nam, H.G. ; Lim, I.J. ; Cha, W.S. ;
KSBB Journal, volume 21, issue 6, 2006, Pages 410~413
The composition of two Bokbunja (Rubus coreanus Miquel) wines (one without mushroom extracts (wine A) and the other is with the extracts (wine B)) has been investigated to improve the quality of Bokbunja wine. The content of solid particle, acidity, crude ash, crude protein of the wine A were 10.5%, 1.36%, 0.45% and 0.05% and those of wine B were 7.4%, 0.54%, 0.31%, and 0.22%, respectively. Crude fat and crude fiber were not detected in both samples. To verify the flavor quality of Bokbunja wine, the volatile components from ethylether extracts of two wines were analyzed using GC/FID and GC/MS. A total number of 12 volatile flavor compounds (6 alcohols, 3 ketones, 1 acid, 1 ester and 1 anhydride) were identified in the two Rubus wines. The major volatile compounds of the wines were 2,3-butanediol, 2,5-furanedione, phenylethyl alcohol, and butanedioic acid and they might affect the major role in the unique flavor of Bokbunja wines.
Analysis of Minerals, Amino Acids,and Vitamin of Lespedeza cuneata
Ding, Ji-Lu ; Lim, Ik-Jae ; Lee, Hee-Duck ; Cha, Wol-Suk ;
KSBB Journal, volume 21, issue 6, 2006, Pages 414~417
For developing functional biomaterials, chemical analysis of Lespedeza cuneata including minerals, amino acids and vitamin were investigated. Minerals of L. cuneata were found to be calcium, potassium, magnesium, phosphorus, sodium, iron, manganese, zinc and copper. Among the free amino acid, proline was 33.77 mg% that were 67.2% of free amino acids and essential amino acids were 7.49 mg%. Total amino acids were analyzed as 2,817 mg% and the content of glutamic acid (496.00 mg%) was highest. In case of vitamin, the highest components was vitamin E with 33.03 mg%.
Pretreatment of Feedstock with High Free Fatty Acid
Jeong, Gwi-Taek ; Park, Don-Hee ;
KSBB Journal, volume 21, issue 6, 2006, Pages 418~421
Fatty acid methyl esters, also referred to as biodiesel, have been determined to have a great deal of potential as substitutes for petro-diesel. In order to enhance productivity in the biodiesel production process, feedstocks were previously recommended to be anhydrous, with a free fatty acid content of less than 0.5%. In this study, the effects of several catalysts, methanol molar ratio, catalyst amount, and reaction time on the reduction of free fatty acid level were studied with a simulated feedstock consisting of 20% oleic acid in rapeseed oil. Ferric sulfate was selected as the best catalyst. Increasing the catalyst amount and methanol molar ratio is very effective in decreasing the acid value of the simulated mixture. Our results may provide useful information with regard to the development of more economic and efficient free fatty acid removal system.
Isolation and Characterization of Inhibition Helicobacter pylori from Culture Media of Fomitopsis pinicola
Lee, Jae-Kyoung ; Choi, Seong-Woo ; Hwang, Yu-Hyun ; Park, Hee-Kuk ; Yoo, Jeong-Weon ;
KSBB Journal, volume 21, issue 6, 2006, Pages 422~427
The culture media from Fomitopsis pinicola were extracted by methanol and examined growth inhibition against Helicobacter pylori. The culture media from 8 days fermentation of F. pinicola showed maximum inhibition activity on H. pylori in 0.25 mg as MIC value. The highest activity against H. pylori by MHCS agar diffusion medium by Fp-P1 in 22.7 mm ID among 3 peaks from methanol fraction of 8 days culture media of Fomitopsis pinicola which purified by ion-exchange chromatography. The Fp-P1 from DEAE-Sephadex A-25 have been analysed by TLC as Fp-T1, Fp-T2 and Fp-T3 by ninhydrin staining. Fp-T3 (Rf value : 0.67) was higher activity against H. pylori in 14.4 mm ID. Fp-T3 was identified as single band by HPLC and TLC. It was assumed to aminosugar by BioLC analysis and TLC staining.
Effect of Sulfate Source on Removal Efficiency in Electrokinetic Bioremediation of Phenanthrene-Contaminated Soil
Kim, Sang-Joon ; Park, Ji-Yeon ; Lee, You-Jin ; Yang, Ji-Won ;
KSBB Journal, volume 21, issue 6, 2006, Pages 428~432
This study investigated the effect of sulfate source on removal efficiency in electrokinetic bioremediation which needs sulfate to degrade contaminants by an applied microorganism. The representative contaminant and the applied microorganism were phenanthrene and Sphingomonas sp. 3Y, respectively. When magnesium sulfate was used, the magnesium ion combined with hydroxyl ion electrically-generated at cathode to cause the decrease of electrolyte pH, and then the microbial activity was inhibited by that. When ammonium sulfate and disodium sulfate were used to solve the pH control problem, the pH values of electrolyte and soil solution were maintained neutrally, and also the high microbial activity was observed. With the former sulfate source, however, ammonium retarded the phenanthrene degradation, and so the removal efficiency decreased to 12.0% rather than 21.8% with magnesium sulfate. On the other hand, the latter improved the removal efficiency to 27.2%. This difference of removal efficiency would be outstanding for an elongated treatment period.
In vivo Biological Function of a Fibrinolytic Enzyme after Oral Adminstration
Lee, Young-Hoon ; Lee, Sung-Ho ; Park, Ki-Hoon ; Choi, Young-Ju ; Lee, Sang-Won ; Kim, Cheol-Ho ; Cho, Soo-Jeong ; Gal, Sang-Wan ;
KSBB Journal, volume 21, issue 6, 2006, Pages 433~438
A fibrinolytic enzyme gene (BCF-1) was subcloned to the pEB vector which is high expression vector in the Bacillus host. The enzyme was purified by using FPLC after ammonium sulfate precipitation. The enzyme was oral-administrated to the rat and checked the bleeding time, blood clotting time and fibrinolytic effect of the serum. In the bleeding time retardation test, it was longer about 1.7 fold in the feeding rat than without feeding. The serum of rat feeded with the enzyme had the fibrinolytic activity from 1 hour to 3 hours after oral-administration. After 3 hours from feeding, the fibrinolytic activity was decreased gradually. Also blood clotting time after bleeding was longer than that of control rat. The enzyme could be detected at band of 30,000 Da in the blood by western blotting. The enzyme was not harmful to the all internal organs of the rats. Taken together, the enzyme originated from B. subtilis BB-1 can be a candidate to develop the drug for thrombosis, arteriosclerosis and myocardial infarction.
The Evaluation of Biocompatigbility of Collagen/Chondroitin Sulfate Sponge as a Scaffold for Corneal Stromal Layer
Jang, In-Keun ; Ahn, Jae-Il ; Seo, Yeong-Gwon ; Kim, Jae-Chan ; Song, Kye-Yong ; Park, Jung-Keug ;
KSBB Journal, volume 21, issue 6, 2006, Pages 439~443
Biocompatibility and tissue regenerating capacity are essential characteristics in the design of collagenous biomaterials for tissue engineering. Attachment of glycosaminoglycans to collagen may add to these characteristics by creating an appropriate micro-environment. In this study, porous type I collagen matrices were crosslinked using dehydrothermal treatment and 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide, in the presence and absence of chondroitin sulfate (CS). The scaffold like discs in 3 mm diameter were inserted into the intralamellar stromal pockets of rabbit cornea. In 8 weeks of follow up, clinical evaluation including corneal neovascularization, opacity and transparency of the graft scaffold was performed, and the inflammatory reaction and migration of corneal fibroblast were evaluated histologically. No inflammation, neovascularization and opacity in any of the implant were observed. CS increased the corneal fibroblast invasion and the transparency. It is concluded that the type I collagen sponge showed a biocompatibility in corneal stromal layer and addition of CS slightly improved the quality of the bioartificial corneal stromal layer. These results could be useful for the development of corneal substitutes.
Skin Anti-aging Effect of Forsythia viridissima L. Extract
Kim, Mi-Jin ; Kim, Ja-Young ; Jung, Teak-Kyu ; Choi, Sang-Won ; Yoon, Kyung-Sup ;
KSBB Journal, volume 21, issue 6, 2006, Pages 444~450
Skin anti-aging effect of Forsythia viridissima L. extract was evaluated by using antioxidant assay, expression of type I procollagen, and UVA-induced matrix metalloproteinase-1 in human dermal fibroblasts. Matairesinol-rich Forsythia viridissima L. extract was showed the scavenging activity of radicals and reactive oxygen species with the
against 1,1-diphenyl-2-picrylhydrazly radical and
against superoxide radicals in the xanthine/xanthine oxidase system, respectively. The type I procollagen was increased 33.76% by treatment with matairesinol-rich Forsythia viridissima L. extract, and UVA-induced MMP-1 was reduced 35.78% in a dose dependent manner. In the human skin irritation test, 2% matairesinol-rich Forsythia viridissima L. extract did not show any adverse effect. Also, the clinical study indicated that a cream group treated with 0.2% matairesinol-rich Forsythia viridissima L. extract significantly reduced skin wrinkles, as compared with a non-treated cream group (p < 0.05). These results suggest that Forsythia viridissima L. extract may be useful as a potential source of functional anti-aging cosmetics.
Isolation and Characterization of An Alcohol Fermentation Strain from Anaerobic Acid Fermentor to Treat Food Wastes
Kim, Jung-Kon ; Han, Gui-Hwan ; Yoo, Jin-Cheol ; Seong, Chi-Nam ; Kim, Seong-Jun ; Kim, Si-Wouk ;
KSBB Journal, volume 21, issue 6, 2006, Pages 451~455
An efficient pilot scale (10 ton) three-stage methane fermentation system to digest food waste has been developed in this laboratory. This system consisted of three stages: semianaerobic hydrolysis, anaerobic acidogenesis and strictly anaerobic methanogenesis. From the secondary acidogenesis reactor, a novel strain KA4 responsible for alcohol fermentation was isolated and characterized. The cell was oval and its dimension was
. This strain was identified as Saccharomyces cerevisiae KA4 by 26S rDNA D1/D2 rDNA sequence. Optimal culture temperature was
. Cells were tolerant to 5% (v/v) ethanol concentration, however, were inhibited significantly by higher ethanol concentration up to 7%. The strain could grow well up to 50% (w/v) initial glucose concentration in the YM liquid medium, however, optimal concentration for ethanol fermentation was 10%. It could produce ethanol in a broad initial pH range from 4 to 10, and optimal pH was 6. In this condition, the strain converted 10% glucose to 7.4% ethanol during 24 hr, and ethanol yield was estimated to be 2.87 moi EtOH/mol glucose.
Stabilization of Alcohol Oxidase under Electrostimulation; Sugars, Hydrogels and Surfactants Effect
Kim, Beom-Su ; Lee, Kang-Min ; Biellmann, J.F. ; Kim, Kyung-Suk ;
KSBB Journal, volume 21, issue 6, 2006, Pages 456~460
We investigated the activity and stability of alcohol oxidase from Hansenula sp., Pichia pastoris, and Candida boidinii under the electric stimulation. The activity and stability of alcohol oxidase depended on electric output voltage, electric stimulation time. This inactivation of the enzyme under electric stimulation could be recovered by stabilizing additives such as sugars, surfactants and hydrogels. These alcohol oxidase was more stable in trehalose, Triton X-100, Brji solution and alcohol oxidase from Hansenula is more stable than that from P. pastoris, and C. boidinii. The stabilizing of enzymes against electric stimulation showed a great potential use of enzymes in biotechnology and medical engineering fields.
A Study on the Protein Productivity of the Promoters for Cold Inducible Genes in Escherichia coli
Kim, So-Yeon ; Kim, Su-Hyun ; Heo, Mi-Ae ; Lee, Sun-Gu ;
KSBB Journal, volume 21, issue 6, 2006, Pages 461~465
We investigated the protein productivity of the promoters for genes showing prolonged induction upon cold shock in Escherichia coli. Six low temperature inducible genes (frdA, glpB, hypB, katG, nupG, ompT) were selected based on the previously reported cDNA microarray based global transcription profiling of Escherichia coli Kl2 in response to cold shock. Their promoter regions were isolated from the genomic DNA of E. coli JM109 and expression levels induced by the promoters were examined by using green fluorescence protein (GFP) as a reporter at
. Among the six promoters, the promoter for nupG showed the highest and prolonged expression at both temperatures and the cold inducibility of nupG promoter was not observed.
Mechanisms of Insulinotropic Effect of YHB-2017 [Genistein] Isolated from fermentation Broths of Streptomyces sp.
Kwag, Won-Jae ; Park, You-Hoi ; Park, Jun-Chul ; Lee, Byung-Kyu ; Kang, Yup ; Choe, Tae-Boo ;
KSBB Journal, volume 21, issue 6, 2006, Pages 466~473
Impaired insulin secretion from pancreatic beta-cells in response to glucose is an important feature in the pathology of non-insulin-dependent diabetes mellitus (NIDDM). In the course of screening for useful insulin secretagogues, we have isolated and identified YHB-2017 (Genistein) as a insulin secretion potentiator from fermentation broths of our in-house microbial library. The insulinotropic activity of YHB-2017 in isolated rat pancreatic islets was exerted only at high concentration of glucose (8.3-16 mM) but not at low concentration of glucose (3.3-5.5 mM). Also, in perifusion study with isolated rat pancreatic islets, YHB-2017 stimulated insulin secretion in a time-dependent manner when YHB-2017 was added to KRB buffer containing 16 mM glucose. In the presence of
diazoxide and 35 mM KCI, which stimulates maximum
influx independently of KATP channel, YHB-2017 enhanced KATP channel-independent insulin secretion at high concentration glucose (16 mM). To elucidate the mechanisms of the glucose-dependent potentiation effect of YHB-2017, pharmacologic inhibitors for protein kinase A, protein kinase C and calcium/calmodulin kinase II were pre-treated and then the potentiation effect of YHB-2017 on insulin secretion was investigated. Pre-treatment of H89 as a PKA inhibitor had a significant inhibitory effect on YHB-2017-induced potentiation effect. Furthermore, western immunoblotting analyses revealed that YHB-2017 increased phosphorylation of PKA substrates and cAMP response element-binding protein (CREB) under high concentration of glucose. These results demonstrated that the insulinotropic effect of YHB-2017 is mediated through PKA signal pathway and activated amplifying
channel-independent insulin secretion pathway.
Ethanol Production by Synchronous Saccharification and Fermentation using Food Wastes
Han, Hyo-Jung ; Li, Hong-xian ; Kim, Seong-Jun ;
KSBB Journal, volume 21, issue 6, 2006, Pages 474~478
For the economically feasible production of ethanol, utilization of SFW (saccharified food wastes) as substrate for synchronous saccharification and fermentation (SSF) process was developed in this study. When 200 g of food wastes and 40 mL of enzyme (
) were reacted, production rate of reducing sugar was
, and consumption rate was
So suitable condition of SSF was concluded at temperature of
. Also, optimal enzyme concentration of SSF was concluded in
, at this condition, the production rate of reducing sugar was
At SSF process, when 50 g of food wastes was supplied in 12 h interval,
of ethanol and 0.45 g-ethanol/g-reducing sugar in yield were obtained in 120 h fermentation. Thus, the technology of high yield of ethanol production using food wastes was confirmed. And semi-continuos SSF system for cutting off cost of enzymatic saccharification was developed in this study.
Optimization of Medium Components for Cell Mass Production of Saccharomyces cerevisiae JUL3 using Response Surface Methodology
Kim, Young-Hwan ; Kang, Seong-Woo ; Lee, Jong-Ho ; Chang, Hyo-Ihl ; Yun, Cheol-Won ; Paik, Hyun-Dong ; Kang, Chang-Won ; Kim, Seung-Wook ;
KSBB Journal, volume 21, issue 6, 2006, Pages 479~483
], one of the cell wall components, is most plentiful polysaccharides in cell wall and has several advantages in immune system. In yeast
is mainly contained in the yeast cell wall, and thus it is important to produce high levels of cell mass for the mass production of yeast
. The best carbon and nitrogen sources on cell mass production were high fructose syrup and yeast extract. Response surface methodology (RSM) was very potential tool for the optimization of process factor and medium component. It was applied to estimate the effects of medium components on the production of cell mass. Optimal concentrations of high fructose syrup and yeast extract by response surface methodology were 8.0% (v/v) and 5.2% (w/v), respectively and the cell mass predicted was
at 20 h of cultivation.
Separation of 6-Gingerol from Ginger [Zingiber officinale Roscoe] and Antioxidative Activity
Lee, Bong-Soo ; Ko, Meyong-Seok ; Kim, Hyun-Jong ; Kwak, In-Seob ; Kim, Dong-Ho ; Chung, Bong-Woo ;
KSBB Journal, volume 21, issue 6, 2006, Pages 484~488
Ginger (Zingiber officinale) is widely used as a dietary condiment throughout the world. Its major constituent, 6-gingerol, exhibits diverse pharmacological activities including anti-oxidant and anti-tumor. Ginger were extracted by 0% to 95% ethanol. Maximum yield of 6-gingerol was obtained with 80% ethanol as extracting solvent at
. We obtained increased yield (7%) of extraction by pretreatment with ultrasonication. Gingerols in the crude ginger extract was isolated by Sepacore preparative liquid chromatography on silica gel. We got the 6-gingerol which weight is 0.53 mg/mL, from fraction F9. Antioxidant effect of 6-gingerol were detected by DPPH moth(10. Its radical scavenging activity was
which compared with ascorbic acid.
Development of Transient Expression System Using Transformed Seedlings of Brassica napus var. napus
Shin, Dong-Il ; Park, Hee-Sung ;
KSBB Journal, volume 21, issue 6, 2006, Pages 489~492
For molecular breeding purpose, genetic transformation of Brassica napus cultivars has been extensively performed using Agrobacterium method. B. napus cv. napus, one of major oil crops, can be transformed via Agrobacterium-based method. We demonstrated that Agrobacterium-mediated transformation via vacuum infiltration slightly worked for the seedlings of B. napus cv. napus according to fluorometric GUS enzyme analysis. In contrast, transformation efficiency was highly enhanced when the seedlings, prior to agroinfiltration, were treated with sodium hydrosulfite solution as a chemical wounding agent. GUS gene expression in transformed seedlings that was confirmed by RT-PCR suggests their usefulness for the development of transient expression system.
Two Stage Fermentation of Xylose/Glucose Mixture for xylitol Production by Candida mogii
Baek, Seoung-Chul ; Kwon, Yun-Joong ;
KSBB Journal, volume 21, issue 6, 2006, Pages 493~497
Two stage fermentations of glucose/xylose mixture which is similar composition with rice straw hemicellulose hydrolysate were performed by Candida mogii ATCC 18364. In first stage, glucose was consumed rapidly for cell growth in aerobic condition (2 vvm, 300 rpm), then D-xylose was used for xylitol production in semi-aerobic condition (1 vvm, 300 rpm). After 4 days of fermentation, about
xylitol was produced with a yield of 0.58 g/g and volumetric productivity of
. To improve the xylitol yield by reduction of xylose consumption for cell growth and maintenance, D-glucose was continuously supplemented during the second stage of fermentation. By D-glucose feeding of
day, xylitol was produced up to
with a yield of 0.8 g/g and volumetric productivity
which are 1.2-1.3 times higher than those obtained without D-glucose feeding.