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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 22, Issue 6 - Dec 2007
Volume 22, Issue 5 - Oct 2007
Volume 22, Issue 4 - Aug 2007
Volume 22, Issue 3 - Jun 2007
Volume 22, Issue 2 - Apr 2007
Volume 22, Issue 1 - Feb 2007
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Exo-Polysaccharide Production from Liquid Culture of Lentinus edodes
Lee, Hee-Hwan ; Cho, Jae-Youl ; Hong, Eock-Kee ;
KSBB Journal, volume 22, issue 1, 2007, Pages 1~6
The optimum liquid culture conditions were investigated for cell growth and polysaccharide production from liquid culture of Lentinus edodes. In flask culture, the optimal medium compositions for the polysaccharide production contained glucose 60 g/L, yeast extract 10 g/L,
2.0 g/L, and
1.0 g/L. The maximum mycelial growth and polysaccharide production were 11.01 g/L and 1.64 g/L, respectively. In bioreactor, through the variation of aeration in order to increase mycelial growth and polysaccharide production, the maximum mycelial growth and polysaccharide production were 55.9 g/L at 8th day and 7.34 g/L at 7th day of cultivation with 1.5 vvm, respectively.
The Development of Whitening Cosmetic Ingredient Having Activity of Melanin Degradation
Kang, Whan-Koo ; Hwang, Sun-Duk ; Kim, Hyoung-Sik ; Jeung, Jong-Sik ; Lee, Bheong-Uk ;
KSBB Journal, volume 22, issue 1, 2007, Pages 7~15
Extensive research was carried out for inhibition of melanin formation as development of whitening cosmetic ingredients. But degradation of melanin itself was not intensively pursued as development of cosmetics. In this study, novel melanin degradation enzyme was developed and characterized. Also this enzyme production process was optimized and formulation was tried using micro encapsulation technique.
The Development of Expression Process Leading to Ethanol Production with Highly Active Cellulase Modified by Directed Evolution
Kang, Whan-Koo ; Jeung, Jong-Sik ; Kim, Hyang-Sik ; Kim, Bum-Change ; Yun, Ji-Sun ; Park, Hyang-Su ;
KSBB Journal, volume 22, issue 1, 2007, Pages 16~21
Although Energy demands of modern society increase rapidly, current energy would be exhausted shortly. Therefore development of bio-ethanol production process from cellulose containing materials was extremly demanded. Therefore development of highly functional cellulase is requisite for this purpose. In this study cellobio-hydrolase (CBH1) gene from Trichorderma reesei was used to increase cellulase activity by directed evolution and highly functional cellobio-hydrolase was obtained and characterized.
Some In-Vitro and In-Vivo Biological Activities of Hot Water Extracts from Fruit Body and Cultured Mycelium of Hericium erinaceum
Jung, Jae-Hyun ; Lee, Kwang-Ho ; Lee, Shin-Young ;
KSBB Journal, volume 22, issue 1, 2007, Pages 22~29
The water-soluble materials extracted from fruit bodies and mycelium of H. erinaceum were prepared. In-vitro anticancer activities on cancer cells and In-vivo proliferation effect on mouse peritoneal exudate cell and spleen cell of samples were investigated. Also, nitric oxide (NO) generation of peritoneal exudate cell, IL-2 production capacity of spleen cells and phagocytic activity of peritoneal macrophages were examined. The water extracts of H. erinaceum suppressed the proliferation of cancer cell (HeLa, Raw264.7, Jurkat, KATO3, EL4, LyD9) with concentration-dependent. The water extract from fruit body showed better suppression effect than that from mycelium in most of cancer cells used. The anticancer effect of water extract of fruits body in the range of 0.01 and 10 mg/ml for Raw 264.7 and EL4 cell lines were the same as the Taxol with one thousandth equivalent of fruit body concentration. Water extracts of fruit body and liquid-cultured products of H. erinaceum induced nitric oxide (NO) generation of peritoneal exudate cell and increased NO generation by stimulus of lipopolysaccharide. Water extracts alone did not induce the proliferation and IL-2 production capacity of spleen cells. However, spleen's proliferation and IL-2 production were induced significantly by the addition of lipopolysaccharide and Con A (concanavalin A) or Con A alone, and the effectiveness of mycelium extract with water were more active than those from fruit body.
AChE Inhibitory Effect and Antioxidative Activity of Submerged Cultured Products from Hericium erinaceum
Jung, Jae-Hyun ; Lee, Shin-Young ;
KSBB Journal, volume 22, issue 1, 2007, Pages 30~36
The water-soluble or ethanol-soluble materials extracted from fruit bodies and cultured products (mycelium and broth) of H. erinaceum were prepared, and their inhibitory effect on acetylcholinesterase (AChE) activity from Electrophorous electricus was investigated. Inhibition of 75-85% for AChE activity at concentration of 10 mg/ml was obtained and the mechanism was due to general non-competitive inhibition. Especially, the supernatant of culture broth by ethanol treatment, exhibited a strong inhibition activity of 94% at 10 mg/ml. The samples from fruit body, mycelium and broth (supernatant and precipitate by ethanol treatment) which were extracted from H. erinaceum, were very effective to inhibit the initial stage oxidation of a linoleic acid at concentration of 0.1 mg/ml. The antioxidative activity of these samples were superior than rutin, vitamin C and tocopherol as antioxidative standards by FTC (ferric thiocyanate) method, and also showed the very strong antioxidative activity of 95% without significant difference of the samples by TBA-RS (thiobarbituric acid-reactive substance) method.
Variation in the Lipid Class and Fatty Acid Composition of Thraustochytrium aureum ATCC 34304
Jeh, Eun-Jin ; Song, Sang-Kyu ; Seo, Jeong-Woo ; Hur, Byung-Ki ;
KSBB Journal, volume 22, issue 1, 2007, Pages 37~42
The heterotrophic marine algae Thraustochytrium aureum ATCC 34304 produces substantial amount of polyunsaturated fatty acids (PUFAs), especially docosahexaenoic acid (DHA). In this study, changes in the lipid and fatty acid compositions of Thraustochytrium aureum ATCC 34304 were investigated according to the growth stage. The major lipids of Thraustochytrium aureum ATCC 34304 were found to be composed of triacylglyceride (TAG), phospholipid (PL), and sterol (ST). The content of triacylglyceride increased during the exponential phase of cell growth, but the content of phospholipid decreased. The composition of total polyunsaturated fatty acids decreased from 60.3% to 45.3% and that of docosahexaenoic acid from 42.1% to 33.9% in the triacylglyceride. The composition of total saturated fatty acids, however, increased from 24.9% to 27.8%. The content of total polyunsaturated fatty acids decreased greatly from 48.0% to 17.5% but the decrease in the content of saturated fatty acids was slight in phospholipid.
Influence of Surfactant on the Hydrolysis of Used Newspaper
Kim, Sung-Bae ; Shin, Hae-Joong ; Kim, Chang-Joon ; Bak, Young-Cheol ;
KSBB Journal, volume 22, issue 1, 2007, Pages 43~47
The effect of surfactant on the hydrolysis of used newspaper was investigated. The most suitable surfactant for the pretreatment stage was found to be NP-series surfactants among 9 kinds of non ionic surfactants. Process parameters such as surfactant concentration, mixing speed, pretreatment temperature and time were tested to optimize for maximum digestibility and 0.5%, 100rpm, 30
, and 1 h were found to be optimum, respectively. In order to maximize digestibility, substrate was pretreated with NP-20 and then the pretreated substrate was hydrolyzed by adding TW-80. The effect of surfactant on the hydrolysis of previously surfactant-pretreated newspaper was marginal. Therefore, the digestibility with the addition order of enzyme and surfactant was investigated by using surfactant only in hydrolysis stage. The results show that digestibility was more lowered as the surfactant addition after adding enzyme to substrate was more delayed.
The Early Detection of the Protein Toxin using Sanification and Fluorescent Dye in the Field
Ha, Yeon-Chul ; Choi, Ki-Bong ; Kim, Seong-Joo ; Choi, Jung-Do ;
KSBB Journal, volume 22, issue 1, 2007, Pages 48~52
This study was carried out to establish the optimum disruption condition of a sonificator for the protein toxin for the purpose of developing automatic biological agent detector equipped a sonificator. One of the best-known collisional quenchers is molecular oxygen, which quenches almost all known fluorophores. The sonification does an excellent job of degassing, which decreased the quenching effect and increased the fluorescence quantity. The fluorescence measurement for the protein using 0.7 X fluorescent dye concentration and above must be done in 1 minute and the fluorescence measurement for the protein using 0.3 X fluorescent dye concentration and below has to be done between 2 and 3 minute. The fluorescence quantity of the sonificatied protein sample was much higher that of the non-sonificatied protein sample. Sonificating the sample turned out to be favorable for the fluorescence measurement when measuring at the low protein concentration.
Biochemical Characterization of Lectin Purified from Kidney Bean Seedling
Roh, Kwang-Soo ;
KSBB Journal, volume 22, issue 1, 2007, Pages 53~57
We have studied biochemical characterization of lectin purified from kidney bean seedling through PBS extraction,
precipitation, and Sephadex G-100 affinity chromatography. The lectin was agglutinated by rabbit erythrocytes. This lectin analyzed by SDS-PAGE is a tetramer composed of two subunits with molecular weights of 46 and 44 kDa. The optimal temperature and thermal stability of the lectin was 30
, respectively. The maximal pH of this lectin was pH 8.2.
Insecticidal Effect of Dermatoohagoides pteronyssinus using Ginkgo biloba Leaves Extracts
Lee, In-Hwa ; Park, Ju-Young ; Choi, Seung-Hyun ;
KSBB Journal, volume 22, issue 1, 2007, Pages 58~61
A Insecticidal effect of Ginkgo biloba leaves extract was conducted for Dermatophagoides pteronyssinus as a predominant species in korea. D. pteronyssinus has been cultured in constant temperature and humidity chamber at the 25
and 75% of relative humidity. The mortality of mites was determined by counting the dead bodies for every hour within 48 hours with pin hall microscope after treated by Ginkgo biloba leaves extract spreaded on 0.1 g of mass cultured media. The sequence of mortality for D. pteronyssinus are as follows, bilobalide was 91.6%, 80
water extract was 82.8%, second water fraction was 75%, ethyl acetate fraction from 80
water fraction was 73%, first ethyl acetate fraction from 80
water extract was 69.4%, putaltrin was 65%, distilled water was 58%, methanol extract was 57.8%, Ginkgolide-A was 57.1 %, ethyl acetate fraction of 80
water extract was 55%, respectively. From the these results we conclude that the bilobalide is the most effective component in the Ginkgo biloba leaves extract having insecticidal effect on house dust mite.
Development of L-Threonine Producing Recombinant Escherichia coli using Metabolic Control Analysis
Choi, Jong-Il ; Park, Young-Hoon ; Yang, Young-Lyeol ;
KSBB Journal, volume 22, issue 1, 2007, Pages 62~65
New strain development strategy using kinetic models and metabolic control analysis was investigated. In this study, previously reported mathematical models describing the enzyme kinetics of intracellular threonine synthesis were modified for mutant threonine producer Escherichia coli TF5015. Using the modified models, metabolic control analysis was carried out to identify the rate limiting step by evaluating the flux control coefficient on the overall threonine synthesis flux exerted by individual enzymatic reactions. The result suggested the production of threonine could be enhanced most efficiently by increasing aspartate semialdehyde dehydrogenase (asd) activity of this strain. Amplification of asd gene in recombinant strain TF5015 (pCL-
-asd) increased the threonine production up to 23%, which is much higher than 14% obtained by amplifying aspartate kinse (thrA), other gene in threonine biosynthesis pathway.