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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 22, Issue 6 - Dec 2007
Volume 22, Issue 5 - Oct 2007
Volume 22, Issue 4 - Aug 2007
Volume 22, Issue 3 - Jun 2007
Volume 22, Issue 2 - Apr 2007
Volume 22, Issue 1 - Feb 2007
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Enantioconvergent Hydrolysis of Racemic Epoxides for Production of Enantiopure Epoxides and Vicinal Diols using Epoxide Hydrolases
Lee, Eun-Yeol ;
KSBB Journal, volume 22, issue 3, 2007, Pages 123~128
One drawback of conventional kinetic resolution of racemic epoxides by epoxide hydrolase (EH) is that the theoretical yield can never exceed 50%. This 50% limitation can be overcome by using enantioconvergent process, in which both enantiomers of the racemic epoxide are transformed via stereochemically matching pathways into a single enantiopure diol as the sole product in 100% theoretical yield. In order to make a single enantiopure vicinal diol, the two enantiomers of the racemic epoxide must be hydrolyzed with retention and inversion of configuration each other. The EHs should be enantio- and regiospecific at the same time. The enantioconvergent hydrolysis with EHs and relevant biotransformation for preparing enantiopure epoxides and vicinal diols with a high yield are reviewed.
Treatment of Organic Waste with Microorganisms of Mixed Population
Kim, Gi-Eun ;
KSBB Journal, volume 22, issue 3, 2007, Pages 129~133
This study represents that a removal efficiency of organic matters in wastewater is activated by a sludge process using new mixed microbial population. In case of mixed microorganisms, removal rates of suspended solid (SS), biochemical oxygen demand (BOD) and chemical oxygen demand (COD) were over 90 percent under experimental condition, and removal efficiency of organic matters, sludge density index (SDI) and capillary suction time (CST) in mixed population were higher than that in not-mixed microorganism, while total kjeldahl nitrogen (TKN) and total phosphorus (T-P) which indicate a degree of eutrophication were removed easily in both case. From these results, we may propose that an application of the mixed microbial population is useful to treat domestic wastewater including a great deal of organic matters.
Reductive Dechlorination of Chlorinated Phenols in Bio-electrochemical Process using an Electrode as Electron Donor
Jeon, Hyun-Hee ; Pak, Dae-Won ;
KSBB Journal, volume 22, issue 3, 2007, Pages 134~138
It was investigated whether an electrode could serve as an electron donor for biological reductive dechlorination of chlorinated phenols in the bio-electrochemical process. There was no dechlorination in the absence of current and scanning electron microscope image showed that the electrode surface was covered with microorganisms. As a result, the electrode attached cells was responsible for reductive dechlorination. Also, initial high chlorinated phenol concentration such as
was rapidly reduced within 5 hours. The maximum dechlorination rate using Monod equation was
(electrode surface area)) in the bio-electrochemical reactor.
Selection of Oriental Medicinal Plants for Screening of Anticancer Agents
Park, Keun-Hyoung ; Kim, So-Young ; Chae, Hee-Jeong ;
KSBB Journal, volume 22, issue 3, 2007, Pages 139~145
An oriental medicinal database was used from medicinal plants for screening of anticancer agents. The prescription frequency and dosage of medicinal plats in 170 anticancer prescriptions were analyzed. From the total prescription score of each medical plant, it was found that high-scored medicinal plants have been widely studied in the previous research on the cancer treatment. Consequently, dangui, hwangui, banha, bachul, jinpy, insam, bacjakyak, deahwang, chungoong, jimo, chunnamsung, omija, hyunggae, huekchook, banggi, boclyung, osooyou were selected as raw materials for the screening of anticancer agents.
Preparation and Characterization of Poly[3-hydroxybutyrate-co-4-hydroxybutyrate] Microsphere
Kang, Hye-Su ; Kim, Beom-Soo ;
KSBB Journal, volume 22, issue 3, 2007, Pages 146~150
Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] microspheres were prepared using solvent evaporation technique. P(3HB-co-4HB) with 3.9 mol% 4HB was synthesized by fed-batch culture of Ralstonia eutropha. The effects of concentration and type of surfactant (Tween 80, sodium dodecylsulfate, and polyvinyl alcohol), addition of dispersion stabilizer (Acacia), concentration of polymer and model drug (bovine serum albumin) on particle size of the microspheres and their in vitro drug release characteristics were investigated. The average particle size of the microspheres decreased with the addition of dispersion stabilizer and increased with the concentration of surfactant, drug and polymer. Amount of drug release increased with decreasing particle size of the microspheres.
Characteristic Study for Methyl-mercaptain Removal by an Essential Oil
Park, Young-Gyu ;
KSBB Journal, volume 22, issue 3, 2007, Pages 151~156
Increasing public concerns over odors and air regulations necessitates the remediation of a wide range of odorous compounds for industrial purpose. Currently, wet scrubbing technique by neutralization using essential oils is utilized to treat methyl mercaptan odor. The chemical analysis is performed to analyze the composition of an essential oil by GC-MS. The objective of this study is to clarify the possibility of the neutralization of odors sprayed in the fixed bed and determine the removal efficiencies in the misty aerosol by different input odor concentration. It is found that methyl mercaptan is significantly removed in the wet scrubber, and their removal efficiency of methyl mercaptan is obtained by 98%.
Bacterial Degradation of Monoethanolamine
Hyun, Jun-Taek ; Rhee, In-Hyoung ; Kwon, Sung-Hyun ; Kim, Dong-Jin ; Cho, Dae-Chul ;
KSBB Journal, volume 22, issue 3, 2007, Pages 157~161
This study is to investigate the biological degradation and the characteristics of MEA, a pH regulator to be put in the cooling water circulation system for power plants, loading to elevate concentrations of COD and N when eluted into the water environment. MEA,
and CODcr were monitored in flask cultures and in a batch aerator. MEA was found to be biologically degradable, producing substantial amount of ammonia (max. 78.1%) in a form of
and other carboneous intermediates. The degradation reaction rates were similar one another over all MEA concentrations tested as the activated sludge (microbial consortium) was acclimated to MEA with the gradual and stepwise increase in MEA input into the batch aerator. Also, MLVSS kept increasing with increasing MEA input. The COD-based degradation reaction order was determined to be 1.
Ammonia Gas Removal by Bacillus subtilis IB101 and Dctimization of Culture Media
Kim, So-Young ; Noh, Yong-Ho ; Kang, Sung-Gak ; Kim, Young-Bum ; Jang, Woo-Jin ; Kim, Dong-Joon ; Yun, Hyun-Shik ;
KSBB Journal, volume 22, issue 3, 2007, Pages 162~167
Ammonia gas is one of the major pollutants which cause environmental pollution and damage to the human and the livestock. The objective of this study was to investigate the important parameters for the development of efficient removal of ammonia gas by Bacillius subtilis IB101 and to optimize the medium composition for the mass production of B. subtilis IB101. The ammonia gas removal efficiency was evaluated at different growth phases and by changing culture conditions (temperature, pH). The effect of
concentration in preculture medium was examined. Medium optimization for the mass production of B. subtilis IB101 was performed by using Plackett-Burman design and one factor at a time method. The removal of ammonia gas was more efficient at exponential phase by 20% than at stationary phase. The ammonia gas removal was the highest at pH 4 and 30
. There was not any significant influence of concentration of
on the removal of ammonia gas. The components of optimized medium for the production of viable Bacillus subtilis IB101 was yeast extract 10 g/l, soluble starch 2.5 g/l,
0.75 g/l, soy bean meal 8 g/l.
Fluorescence Detection for Protoporphyrin IX Induced from 5-ALA and ALA-methyl ester in Incubated Liver Cancer Cells
Kim, Myung-Hwa ; Kim, Jung-Mi ; Kim, Hyun-Jeong ; Lee, In-Seon ; Kim, Kyung-Chan ; Lee, Chang-Seop ;
KSBB Journal, volume 22, issue 3, 2007, Pages 168~173
To clarify the usefulness of fluorescent diagnosis for cancer, we investigated the optimal method of administrating 5-aminolevulinic acid (5-ALA), 5-aminolevulinic acid methyl ester (ALA-methyl ester) by analyzing fluorescence signal of Protoporphyrin IX (PpIX) in the cultured normal and cancer cells. 5-ALA and ALA-methyl ester was injected as a photosensitizer to the cancer liver cells (HepG2) and normal liver cells (Chang). Chang and HepG2 cells were incubated with various concentrations of 5-ALA and ALA-methyl ester (0-800
). The accumulation of PpIX induced by 5-ALA and ALA-methyl ester was in HepG2 and Chang. The cell viability was measured by MTT assay. Fluorescence of PpIX in HepG2 cell was excited at a wavelength (
= 410 nm) and showed an emission spectrum at 603.2 nm, 660.8 nm and 603.2 nm, 661.4 nm which could be related to the PpIX generation induced by the applied 5-ALA and ALA-methyl ester, respectively. The experimental results showed that fluorescence signal of PpIX was proportional to the concentration of 5-ALA and ALA-methyl ester in tumor cells, but measured with low concentration in normal cells. Another results showed that the PpIX formation rate induced by ALA-methyl ester is higher than that of 5-ALA.
Extraction and Identification of Volatile Isothiocyanates from Wasabi using Supercritical Carbon Dioxide
Kim, Sung-Jin ; Lee, Min-Kyung ; Back, Sung-Sin ; Chun, Byung-Soo ;
KSBB Journal, volume 22, issue 3, 2007, Pages 174~178
The aim of this study was to identifyisothiocyanates (ITCs) from wasabi (Wasabi japonica Matsum) using supercritical carbon dioxide (
) and to compare the composition in the extracts between
and organic solvents extraction. A semi-continuous high pressure apparatus was used to extract wasabi (roots, stems and leaves) at following conditions pressure 80
120 bar, temperature
. Ether, ethanol, chloroform and dichloromethane were used as organic solvents. The ITCs extracted by means of both separation technologies were analyzed by a gas chromatography system. As the results of study, AITC and ITCs were highly extracted at 40
and 80 bar. To extract AITC from wasabi,
extraction is more effective than organic solvents extraction, resulted in thermal degeneration and remaining of organic solvents.