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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 22, Issue 6 - Dec 2007
Volume 22, Issue 5 - Oct 2007
Volume 22, Issue 4 - Aug 2007
Volume 22, Issue 3 - Jun 2007
Volume 22, Issue 2 - Apr 2007
Volume 22, Issue 1 - Feb 2007
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Genetic Synthesis and Applications of Repetitive Protein Polymers
Park, Mi-Sung ; Choi, Cha-Yong ; Won, Jong-In ;
KSBB Journal, volume 22, issue 4, 2007, Pages 179~184
This study introduces the characteristics and some applications of repetitive polypeptides, especially to the biomaterial, tissue engineering scaffolds, drug delivery system, and DNA separation systems. Since some fibrous proteins, which consist of repeating peptide monomers, have been reported that their physical properties are changed dramatically by means of temperature alteration or pH shifting. For that reason, fibrous protein-mimetic polypeptides, which are produced by the recombinant technology, can be applied to the diverse biological fields. Repetitive polypeptides can also be used in the bioseparation area such as DNA sequencing, because they make DNA separation possible in free-solution electrophoresis by conjugating DNA fragments to them. Moreover, artificial synthesis of repetitive polypeptides helps to demonstrate the correlations between mechanical properties and structures of natural protein polymer, which have been proven that repetitive domains are affected by the sequence of the repeating domains and the number of repeating subunits. Repetitive polypeptides can be biologically synthesized using some special cloning methods, which are represented here. Recursive directional ligation (RDL) and controlled cloning method (CCM) have been proposed as excellent cloning methods in that we can control the number of repetition in the multimerization of polypeptides and the components of repetitive polypeptides by either method.
Effects of Various Stabilizers on the Production of hGM-CSF in Transgenic Nicotiana tabacum Suspension Cell Cultures
Cho, Jong-Moon ; Kim, Dong-Il ;
KSBB Journal, volume 22, issue 4, 2007, Pages 185~190
Productivity of secreted recombinant protein depends largely on its stability in the extracellular environment with protease. Most hGM-CSF produced by transgenic tobacco cell cultures and secreted to the medium was confirmed to be rapidly degraded by protease in medium. To increase the productivity, therefore, various protein stabilizers such as gelling agents such as carrageenan and alginate, polymers, polyols, and amino acids have been tested. The stability of hGM-CSF in spent medium without cells was improved by the presence of gelling agents. However, the reason for the enhanced production by the addition of gelling agents may be due to the increased expression level and permeability rather than stability. The addition of DMSO inhibited the cell growth, but improved specific yield. The others were not effective for stability as well as hGM-CSF production.
A Study on Removal of Organics, Nitrogen and Phoschorus of Domestic Wastewater in Pilot-Scale Upflow Packed Bed Column Reactor
Seon, Yong-Ho ;
KSBB Journal, volume 22, issue 4, 2007, Pages 191~196
This study used biofilm process, which needs simple operation, maintenance and smaller facility area than conventional activated sludge process with the small plant operation, in the treatment of increasing sewage with the rapid industrial growth. The reactor used in this study consists of one anaerobic and one aerobic chamber filled with waste ceramic and waste vinyl as media and the treated sewage was from restaurant source. The experiment was scaled up from lab. to pilot scale and lasted for about 100 days. We focused on the removal efficiency of organics, nitrogen and phosphorus with constant HRT and continuous aeration. The removal efficiency of
and SS were 94.33% and 87.77% respectively, which was a satisfaction level. However the removal efficiency of
was 81.46% somewhat below the desired level of 90%, and that of T-N and T-P showed 71.92% and 21.10% respectively, that was below the expected value. The removal efficiency of
and T-N in the pilot scale was about 10% low compared with the lab.-scale.
Octimization of Conditions of Filtration and Concentration of Methanol Extract for Recovery of Paclitaxel from Plant Cell Culture
Kim, Jin-Hyun ;
KSBB Journal, volume 22, issue 4, 2007, Pages 197~200
This study examined the conditions of filtration and concentration of methanol extract from biomass. Filtration efficiency was improved by adding diatomaceous earth as a filter aid. The optimal amount of diatomaceous earth was 6% (w/w) to reduce the filtration time. The filtration time was reduced by 4.2% in first extraction, 30.0% in second extraction, 22.8% in third extraction, and 19.0% in fourth extraction, respectively. The optimal temperature of water bath was below 50
for preventing paclitaxel degradation during concentration of methanol extract using a rotary evaporator. The temperature of concentrated solution in rotary evaporator was relatively low compared to bath temperature because of latent heat of evaporation. The stopping point of concentration in rotary evaporator for the following step was at a specific gravity of 0.96 of the concentrated solution in terms of the purity and yield of paclitaxel. This information is very useful for mass extraction of biomass for the recovery of paclitaxel from plant cell culture.
L-Arabinose Production from Diluted Sulfuric Acid Hydrolysis of Corn-fiber
Lee, Hyung-Joo ; Lee, Won-Kyu ; Ryu, Yeon-Woo ;
KSBB Journal, volume 22, issue 4, 2007, Pages 201~206
The demand of L-arabinose has been increased recently because of its advantages including clinical effect. L-arabinose can be produced from dilute acid hydrolysis of agricultural wastes. In this study, optimum conditions of L-arabinose production using dilute acid hydrolysis of agricultural wastes and nutshells were determined. Among the tested various agricultural wastes and nutshells, corn fiber was selected as the best raw material for the production of arabinose. The highest arabinose production was achieved an acid hydrolysis of corn fiber for 1 h at 130
with 0.4% sulfuric acid. Above optimal conditions, it was obtained 20.1 g/L glucose, 10.1 g/L xylose, 7.8 g/L arabinose and 1.8 g/L galactose from 90 g/L of corn fiber. For the purification of arabinose, it was carried out to remove all of sugars except arabinose by the Candida tropicalis cultivation of acid hydrolyzate and an organic contaminants such as pigments by the active carbon treatment of fermentation broth. Moreover, experiments were carried out to eliminate an ions by exchange chromatography. Finally, we obtained 3.1 g of partially purified L-arabinose powder with about 40% yield by evaporation and vacuum drying.
Effects of Pluronic F-68 and Oxygen Vectors on the Cell Growth of Angelica gigas Nakai in Aqueous Two-Phase System
Cheon, Su-Hwan ; Lee, Kyoung-Hoon ; Kwon, Jun-Young ; Ryu, Hyun-Nam ; Kim, Dong-Il ;
KSBB Journal, volume 22, issue 4, 2007, Pages 207~212
Pluronic F-68 and oxygen vectors were applied to increase the cell growth of Angelica gigas Nakai in aqueous two-phase system (ATPS). ATPS was composed of 3.6% (w/v) polyethylene glycol (PEG) 20,000 and 2.8% (w/v) crude dextran. n-Hexadecane, n-dodecane and FC-40 were used as oxygen vectors to enhance the oxygen transfer in ATPS. With 2
10 g/L of Pluronic F-68, addition of of n-hexadecane and FC-40 significantly enhanced the oxygen transfer rate as well as the maximum cell mass in a medium with ATPS. However, n-dodecane reduced the cell growth in all treatments. Maximum cell densities were increased up to 27.5% with 10 g/L of Pluronic F-68 and up to 40.2% with 8% (v/v) n-hexadecane compared to those of the controls without Pluronic F-68 and oxygen vectors. It was confirmed that the cell growth could be increased in ATPS using n-hexadecane.
Effects of Hydrophilic Additives on the Release Rate of Protein Drugs
Kwon, Young-Kwan ; Kim, Ji-Hyeon ; Yoo, Young-Je ;
KSBB Journal, volume 22, issue 4, 2007, Pages 213~217
It has been reported that hydrophobic additives generally decrease the release rate of protein drugs from drug delivery systems (DDS) and hydrophilic additives increase the release rate. In many cases, however, the addition of hydrophilic molecule is necessary for improving the stability of protein drugs. In the present work, the effects of hydrophilic additives on the release profiles, and micelle formation of protein drug formulations were investigated to develop a novel method for protein drug delivery. For model protein drug, bovine serum albumin (BSA) was employed and several hydrophilic additives were used in the release experiments. Hydrophilic additive D-sorbitol showed the lower release rates of BSA than other hydrophobic additives due to the gel strengthening ability of the additive and the optimum concentration of D-sorbitol was 3 w/v % for the retarded release rate. In addition, it was found that the addition of D-sorbitol was very effective for obtaining homogeneous and stable DDS. The results were discussed in terms of the micelle formation and the micelle structure, i.e., the differences in gel structure and the distribution of drugs in micelles.
Octimization of Score Production via Sonication of Antifungal Polyene-producing Actinomycetes
Kim, Byung-Kyun ; Han, Kyu-Beom ; Kim, Eung-Soo ;
KSBB Journal, volume 22, issue 4, 2007, Pages 218~221
The polyene antifungal antibiotics, mostly produced by Gram-positive soil actinomycetes, are a family of type I polyketide macrolide ring compounds with 20
40 carbon backbone contain 3
8 conjugated double bonds. Using polyene-specific genomic screening strategy, we previously isolated three novel polyene-producing actinomycetes strains from soil, implying the potential application of these strains' spores as microbial pesticides. Here, we report that the sonication is a very efficient method for actinomycetes spore generation with a sonicator power-dependent manner. In addition, these sonication-driven actinomycetes spores retained significant portion of their cell viabilities as well as antifungal activities after freeze-drying procedure, implying the potential application of these strains' spores as microbial pesticides.
Optimization of Biodiesel Production from Rapeseed Oil Using Response Surface Methodology
Jeong, Gwi-Taek ; Yang, Hee-Seung ; Park, Seok-Hwan ; Park, Don-Hee ;
KSBB Journal, volume 22, issue 4, 2007, Pages 222~227
Biodiesel (fatty acid methyl esters) have used to as substitutes for petro-diesel by mixed-form with petro-diesel. In several processes of biodiesel production, alkali-catalyst transesterification produced to biodiesel of high contents with short reaction time. In this study, we investigate the optimal condition of alkali-catalyst transesterification of rapeseed oil produced at Jeju island in Korea using response surface methodology. The optimal condition of biodiesel production is reaction temperature 59.7
, catalyst amount 1.18%, oil to methanol molar ratio 1:8.75, and reaction time 5.18 min. At that reaction condition, the fatty acid methyl ester contents of product are above 97%. Our results may provide useful information with regard to the development of more economic and efficient biodiesel production system.
Reaction Condition for Biodiesel Production from Animal Fats
Yang, Hee-Seung ; Jeong, Gwi-Taek ; Park, Suk-Hwan ; Park, Jae-Hee ; Park, Don-Hee ;
KSBB Journal, volume 22, issue 4, 2007, Pages 228~233
The high cost and lack of vegetable oil are limiting the expansion of biodiesel production. The purpose of research was to investigate the potential of animal fats as biodiesel feedstock. In this paper, transesterification using alkali catalyst and methanol was performed to reaction, we carried out experiments that it was changed variables as reaction temperature, methanol molar ratio, catalyst types, amount of catalyst and reaction time. The optimum reaction condition for biodiesel production was reaction temperature 65
, potassium hydroxide 1.0% (w/w), oil to methanol molar ratio 1:15 and reaction time 20 min. In this reaction condition, the contents of fatty acid methyl ester was reached to about 98.7%. Also, properties of biodiesel were measured to correspond to domestic quality standard of acid values, density and viscosity.
Tandem Mass Spectrometry of N-linked Glycans from Human Immunoglobulin G
Joo, Hwang-Soo ; Kim, Yun-Gon ; Jang, Kyoung-Soon ; Kim, Byung-Gee ;
KSBB Journal, volume 22, issue 4, 2007, Pages 234~238
We used electrospary ionization ion trap tandem mass spectrometry (ESI-IT tandem MS) to structural elucidation of three different biantennary-type glycans having zero, one, two galactoses (G0, G1, G2). The highest fragment ion in the MS/MS spectra of three glycans was produced by 0,2-ring cleavage of fucose-linked N-acetylglucosamine (GlcNAc) in reducing end. The fragment ions both from precursor ions and 0,2-ring cleaved ions (
; n=5 for G0, n=6 for G1 and G2) were not overlapped each other. As results of
analyses, tandem fragmentation trees of each glycans were generated and 2,4-ring cleavages (
) were occurred in GlcNAc linked to reducing end GlcNAc. This structural elucidation and fragmentation study of N-linked glycans by tandem mass spectrometry can be applied to structural analysis of more complicated glycans.
Preparation of Agar Microcapsules and Analysis of Their Internal Structure
Park, Chul-Wan ; Lee, Shin-Young ; Hur, Won ;
KSBB Journal, volume 22, issue 4, 2007, Pages 239~243
A method of forming agar microcapsule with fibroin coating was developed in this report. The capsules were prepared from a W/O emulsion of hot agar in mineral oil and were subsequently coated by fibroin. The capsules were harvested as precipitated aggregates, which can be dispersed in an aqueous media. The diameter of the microcapsule was less than
by microscopic observation and 90% of them were between
. The structure of the aggregates and their dispersed microspheres were investigated by scanning electron microscope. Confocal microscopy was applied to visualize the core-shell structure of the agar microcapsule with fibroin coating. Thermogravimetric analysis (TGA) measured their composition to be agar 51.2%, fibroin 13.8%, Span 80 1.4% by weight.
Integrated Wet Oxidation and Aerobic Biological Treatment of the Wastewater Containing High Concentration of Phenol
Choi, Ho-Jun ; Lee, Seung-Ho ; Yu, Yong-Ho ; Yoon, Wang-Lai ; Suh, II-Soon ;
KSBB Journal, volume 22, issue 4, 2007, Pages 244~248
The treatment of a model wastewater containing high concentration, 10
, of phenol in an integrated wet oxidation-aerobic biological treatment was investigated. Partial wet oxidation under mild operating conditions was capable of converting the original phenol to biodegradable organic acids such as maleic acid, formic acid and acetic acid, the solution of which was subjected to the subsequent aerobic biological treatment. The wet oxidation was carried out at 150
and the initial pH of 1 to 12. The high temperature of 200
and the acidic initial condition in the wet oxidation led to effluents of which biodegradability was higher in the subsequent biological oxidation process, as assessed by chemical oxygen demand (COD) removal. Homogeneous catalyst of
was also used for increasing the oxidation rate in the wet oxidation at 150
and initial pH of 3.0. However, the pretreatment with the catalytic wet oxidation resulted in effluents which were less biodegradable in the aerobic biological process compared to those out of the non-catalytic wet oxidation at the same operating conditions.
Anti-angiogenic and Anti-cell Adhesion Effects and Their Mechanism with the Extract of Camellia japonica Leaf
Song, Min-Gyu ; Seo, Hyo-Jin ; Moon, Je-Hak ; Park, Keun-Hyung ; Kim, Jong-Deog ;
KSBB Journal, volume 22, issue 4, 2007, Pages 249~254
Anti-angiogenesis and anti-cell adhesion effects were investigated with different dose of Camellia japonica leaf (CJL) extract for applying anti-cancer, anti-metastasis and anti-obesity. Cytotoxicity on HUVECs was very low at 200 ug/mL of CJL-extract. Anti-angiogenic ratio at increasing dose of 1.5 ug/mL, 3.0 ug/mL, 15 ug/mL and 30 ug/mL was showed 30.7%, 38.5%, 53.8%, and 70.0%, respectively. Also, anti-cell adhesion effect at concentration of
was expressed on E-selectin by 46.7%, 66.7%, and 86.76%, on VCAM-1, 23.0%, 61.5%, and 84.6%, and on ICAM-1, 11%, 55.5%, and 88.8%, respectively. For inquiring anti-angiogenesis mechanism, when western blot was performed with different dose of CJL extract, signal molecules of VEGFR-2,
-catenin and PI3-K were suppressed. As the signal transduction from VEGFR-2,
-catenin and PI3-K to NF-
B was interupted, angiogenesis could not be occurred causing not activated NF-kB. C. japonica leaf (CJL) is a useful herb for developing therapeutics of angiogenesis related diseases such as cancer, metastasis, rheumathioid arthritis and obesity.
Determination of the Calcium Contents of Vegetables Sprayed with Liquid Calcium Fertilizer and Fermentation Characteristics of Kimchi using Ca-treated Korean cabbage
Shin, Hyun-Jae ; Lee, Sang-Hwa ; Kim, Bok-Hee ;
KSBB Journal, volume 22, issue 4, 2007, Pages 255~259
Liquid calcium fertilizer evenly dispersed has been prepared using calcium carbonate powder and several surfactants. The calcium contents of nine fruits and vegetables were compared after spraying the liquid fertilizer onto the leaf of them six times for 2 months. The calcium contents of cabbage and potato increased to 155% and 154%, respectively. In addition, by virtue of the relatively high contents of calcium, rigidities of the texture were also increased, which would result in higher value-added vegetables. Kimchi was prepared using a Korean cabbage sprayed with the liquid calcium fertilizer and its fermentation characteristics were analyzed. A sensory evaluation has been performed to give the best result for a calcium Kimchi fermented for 14 days.
Surface Modification of Glass Chip for Peptide Microarray
Cho, Hyung-Min ; Lim, Chang-Hwan ; Neff, Silke ; Jungbauer, Alois ; Lee, Eun-Kyu ;
KSBB Journal, volume 22, issue 4, 2007, Pages 260~264
Peptides are frequently studied as candidates for new drug development. Recently, synthesized peptide library is screened for a certain functionality on a microarray biochip format. In this study, in order to replace the conventional cellulose membrane with glass for a microarray chip substrate for peptide library screening, we modified the glass surface from amines to thiols and covalently immobilized the peptides. Using trypsin-FITC (fluorescein isothiocyanate) conjugate that could specifically bind to a trypsin binding domain consisting of a 7-amino acid peptide, we checked the degree of surface modification. Because of the relatively lower hydrophilicity and reduced surface roughness, the conjugation reaction to the glass required a longer reaction time and a higher temperature. It took approximately 12 hr for the reaction to be completed. From the fluorescence signal intensity, we could differentiate between the target and the control peptides. This difference was confirmed by a separate experiment using QCM. Furthermore, a smaller volume and higher concentration of a spot showed a higher fluorescence intensity. These data would provide the basic conditions for the development of microarray peptide biochips.
In Vitro Glucose and Bile Acid Retardation Effect of Fucoidan from Laminaria japonica
Park, Kap-Yong ; Back, Jin-Hong ; Hur, Won ; Lee, Shin-Young ;
KSBB Journal, volume 22, issue 4, 2007, Pages 265~269
Fucoidan from sea tangle (Laminaria japonica) was isolated by hot water extraction, and partially purified. The in-vitro glucose and bile acid retarding effects of the partially purified fucoidan were investigated. Fucoidan exhibited 27.06
21.42% of retarding index for glucose and 33.50
27.02% of retarding index for bile acid during in vitro dialysis experiment for 2 hours. These retarding effects on glucose and bile acid diffusion was considered as a relatively good or very good, suggesting the prevention from diabetes and arteriosclerosis of some extent.