Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal Basic Information
Journal DOI :
Korean Society for Biotechnology and Bioengineering
Editor in Chief :
Volume & Issues
Volume 22, Issue 6 - Dec 2007
Volume 22, Issue 5 - Oct 2007
Volume 22, Issue 4 - Aug 2007
Volume 22, Issue 3 - Jun 2007
Volume 22, Issue 2 - Apr 2007
Volume 22, Issue 1 - Feb 2007
Selecting the target year
A Research and Application of Polyhydroxyalkanoates in Biosensor Chip
Park, T.J. ; Lee, S.Y. ;
KSBB Journal, volume 22, issue 6, 2007, Pages 371~377
Polyhydroxyalkanoates (PHAs) are a family of microbial polyesters that can be produced by fermentation from renewable resources. PHAs can be used as completely biodegradable plastics or elastomers. In this paper, novel applications of PHAs in biosensor are described. A general platform technology was developed by using the substrate binding domain (SBD) of PHA depolymerase as a fusion partner to immobilize proteins of interest on PHA surface. It could be shown that the proteins fused to the SBD of PHA depolymerase could be specifically immobilized onto PHA film, PHA microbead, and microcontact printed PHA surface. We review the results obtained for monitoring the specific interaction between the SBO and PHA by using enhanced green fluorescent protein, red fluorescent protein, single chain antibody against hepatitis B virus preS2 surface protein and severe acute respiratory syndrome coronavirus surface antigen as model proteins. Thus, this system can be efficiently used for studying protein-protein and possibly protein-biomolecule interactions for various biotechnological applications.
Biochip System for Environmental Monitoring using Nanobio Technology
Kim, Young-Kee ; Min, Jun-Hong ; Oh, Byung-Keun ; Choi, Jeong-Woo ;
KSBB Journal, volume 22, issue 6, 2007, Pages 378~386
Bio-sensing devices, which are basically integrated and miniaturized assay systems consisted of bioreceptor and signal transducer, are advantageous in several ways. In addition to their high sensitivity, selectivity, simplicity, multi-detection capability, and real time detection abilities, they are both very small and require relatively inexpensive equipments. Two core technologies are required to develop bio-sensing devices; the fabrication of biological receptor module (both of receptor development and immobilisation of them) and the development of signal transducing instruments containing signal generation technique. Various biological receptors, such as enzymes, DNA/RNA, protein, and cell were tried to develop bio-sensing devices. And, the signal transducing instruments have also been extensively studied, especially with regard to electrochemical, optical, and mass sensitive transducers. This article addresses bio-sensing devices that have been developed in the past few years, and also discusses possible future major trends in these devices.
Enhancement of DNA Microarray Hybridization using Microfluidic Biochip
Lee, H.H. ; Kim, Y.S. ;
KSBB Journal, volume 22, issue 6, 2007, Pages 387~392
Recently, microfluidic biochips for DNA microarray are providing a number of advantages such as, reduction in reagent volume, high-throughput parallel sample screening, automation of processing, and reduction in hybridization time. Particularly, the enhancement of target probe hybridization by decrease of hybridization time is an important aspect highlighting the advantage of microfluidic DNA microarray platform. Fundamental issues to overcome extremely slow diffusion-limited hybridization are based on physical, electrical or fluidic dynamical mixing technology. So far, there have been some reports on the enhancement of the hybridization with the microfluidic platforms. In this review, their principle, performance, and outreaching of the technology are overviewed and discussed for the implementation into many bio-applications.
Applications and Developmental Prospect of Protein Microarray Technology
Oh, Young-Hee ; Han, Min-Kyu ; Kim, Hak-Sung ;
KSBB Journal, volume 22, issue 6, 2007, Pages 393~400
Analysis of protein interactions/functions in a microarray format has been of great potential in drug discovery, diagnostics, and cell biology, because it is amenable to large-scale and high-throughput biological assays in a rapid and economical way. In recent years, the protein microarray have broaden their utility towards the global analysis of protein interactions on a proteome scale, the functional activity analysis based on protein interactions and post-translational modifications (PTMs), and the discovery of biomarkers through profiling of protein expression between sample and reference pool. As a promising tool for proteomics, the protein microarray technology has advanced outstandingly over the past decade in terms of surface chemistry, acquisition of relevant proteins on a proteomic level, and detection methods. In this article, we briefly describe various techniques for development of protein microarray, and introduce developmental state of protein microarray and its applications.
Application of Electronic Nose in Biotechnology
Lim, Chae-Lan ; Noh, Bong-Soo ;
KSBB Journal, volume 22, issue 6, 2007, Pages 401~408
It's not easy to detect the specific compounds from various compounds that fermented in bioreactor. The electronic nose was an instrument, which comprised of an array of electronic chemical sensors with partial specificity and an appropriate pattern recognition system, capable of recognizing simple or complex volatiles. It can conduct fast analysis and provide simple and straightforward results and is best suited for quality control and process monitoring in field of biotechnology. This review examined the application of electronic nose in biotechnology and brief explanation of its principle. In this minireview numbers of applications of an electronic nose in biotechnology include monitoring fermentation process, to overcome interference with alcohol, and to detect contaminant microorganism were discussed. The electronic nose would be useful for a wide variety of biotechnology when correlating analytical instrumental data with the obtained data from electronic nose.
Polydiacetylene-Based Chemo-/Biosensor of Label Free System with Various Sensing Tools
Park, Hyun-Kyu ; Park, Hyun-Gyu ; Chung, Bong-Hyun ;
KSBB Journal, volume 22, issue 6, 2007, Pages 409~413
Polydiacetylene(PDA)-based sensors possess a number of properties that can be successfully applied for label-free detection system. PDA is one of the most attractive color-generating materials, with growing applications as sensors. Here we introduce various PDA-based devices, used as biosensor, chemosensor, thermosensor, and optoelectronics sensor. In general, PDA liposomes and films are closely packed and properly designed for polymerization via 1,4-addition reaction to form an ene-yne alternating polymer chain. PDA-based two/three dimensional structures have been used for colorimetric or fluorescent devices, sensing biological as well as chemical components. This color-generating material also present a very high charge carrier mobility, allowing its application as field-effect transistor (FET). The immobilized PDA structures or films have distinct advantages for the detection of low concentration target molecules over the aqueous solution-based detection systems. In the present review, reported detection methods by using various PDA structures are summarized with updated references.
Toxicity Monitoring and Assessment of Nanoparticles Using Bacteria
Hwang, Ee-Taek ; Lee, Jung-Il ; Sang, Byoung-In ; Gu, Man-Bock ;
KSBB Journal, volume 22, issue 6, 2007, Pages 414~420
Nanomaterials have been applied to various fields due to their advantageous characteristics such as high surface area, rapid diffusion, high specific surface areas, reactivity in liquid or gas phase, and a size close to biomacromolecules. Up to date, increased manufacturing and frequently use of the materials, however, revoke people's concerns on their hazard impact including toxicity the materials. Many research groups have carried out different protocols to evaluate toxic effects of nanomaterilas on different organisms, and consequently, nanomaterials are known to cytotoxicity. In this paper, we reviewed some of the most reports on toxic effects of several nanoparticles specifically on bacteria. There are numbers of reports focused on antibacterial effect of nanoparticles based on bacterial cell viability. Therefore, the application of each nanomaterial should be concerned with its toxicity and its toxic effect should be evaluated in terms of concentrations and sizes of the nanomaterials used, prior to use of a nanomaterial.
Kim, Tai-Jin ;
KSBB Journal, volume 22, issue 6, 2007, Pages 421~425
Development of an Open Sandwich Fluoroimmunoassay Based on FRET
Wei, Quande ; Lee, Moon-Kwon ; Seong, Gi-Hun ; Choo, Jae-Bum ; Lee, Eun-Kyu ;
KSBB Journal, volume 22, issue 6, 2007, Pages 426~432
We have developed a sensitive, one-step, homogeneous open sandwich fluoroimmunoassay (OsFIA) based on fluorescence resonance energy transfer (FRET) and luminescent semiconductor quantum dots (QDs). In this FRET assay, estrogen receptor-
) antigen was incubated with QD-labeled anti-ER-
monoclonal antibody and AF (Alexa Fluoro)-labeled anti-ER polyclonal antibody for 30 minutes, followed by FRET measurement. The dye separation distance was estimated to be between
. The present method is rapid, simple and highly sensitive, and did not require the bound/free reagent separation steps and solid-phase carriers. A concentration as low as 0.05 nM (2.65 ng/ml) receptor was detected with linearity (
> 0.990). In addition, the assay was performed with commercial antibodies. This assay provides a convenient alternative to conventional, laborious sandwich immunoassays.
Fabrication of Nanopatterns for Biochip by Nanoimprint Lithography
Choi, Ho-Gil ; Kim, Soon-Joong ; Oh, Byung-Ken ; Choi, Jeong-Woo ;
KSBB Journal, volume 22, issue 6, 2007, Pages 433~437
A constant desire has been to fabricate nanopatterns for biochip and the Ultraviolet-nano imprint lithography (UV-NIL) is promising technology especially compared with thermal type in view of cost effectiveness. By using this method, nano-scale to micro-scale structures also called nanopore structures can be fabricated on large scale gold plate at normal conditions such as room temperature or low pressure which is not possible in thermal type lithography. One of the most important methods in fabricating biochips, immobilizing, was processed successfully by using this technology. That means immobilizing proteins only on the nanopore structures based on gold, not on hardened resin by UV is now possible by utilizing this method. So this selective nano-patterning process of protein can be useful method fabricating nanoscale protein chip.
Development of a Biosensor Using Electrochemically-Active Bacteria [EAB] for Measurements of BOD [Biochemical Oxygen Demand]
Yoon, Seok-Min ; Choi, Chang-Ho ; Kwon, Kil-Koang ; Jeong, Bong-Geun ; Hong, Seok-Won ; Choi, Yong-Su ; Kim, Hyung-Joo ;
KSBB Journal, volume 22, issue 6, 2007, Pages 438~442
A biosensor using electrochemically-active bacteria (EAB) enriched in three-electrode electrochemical cell, was developed for the determination of biochemical oxygen demand (BOD) in wastewater. In the electrochemical cell, the positively poised working electrode with applying a potential of 0.7 V was used as an electron acceptor for the EAB. The experimental results using artificial and raw wastewater showed that the current pattern generated by the biosensor and its Coulombic yield were proportional to the concentration of organic matter in wastewater. The correlation coefficients of BOD vs Coulombic yield and
vs Coulombic yield were 0.99 and 0.98, respectively. These results indicate that the biosensor enriched with the EAB capable of transferring electrons directly toward the electrode can be utilized as a water-quality monitoring system due to a quick and accurate response.
Detection of C-Reactive Protein Using Direct-binding Quartz Crystal Microbalance Immunosensor
Kim, N. ; Kim, D.K. ; Cho, V.J. ;
KSBB Journal, volume 22, issue 6, 2007, Pages 443~446
A prognostic indicator of coronary heart disease, C-reactive protein, was tried to be determined by a batch-type quartz crystal microbalance immunosensor. The sensor was operated by direct-binding mode and the optimum concentration for the corresponding antibody for immobilization was
. The reaction buffer for the system was 0.1 M sodium phosphate (pH 7.0) and system operation was performed in the order of baseline stabilization, analyte addition and measurement, and regeneration of the sensor chip with 10 mM NaOH. When plotted in double-logarithmic scale, the sensor showed a linear detection range of 0.27-106.00 nM for rat C-reactive protein with the limit of detection of 0.53 nM. It also showed a good reusability.