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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 23, Issue 6 - Dec 2008
Volume 23, Issue 5 - Oct 2008
Volume 23, Issue 4 - Aug 2008
Volume 23, Issue 3 - Jun 2008
Volume 23, Issue 2 - Apr 2008
Volume 23, Issue 1 - Feb 2008
Selecting the target year
Cellulosic Ethanol Production
Chung, Chang-Ho ;
KSBB Journal, volume 23, issue 1, 2008, Pages 1~7
The world demand of ethanol as an alternative fuel for gasoline is increasing rapidly because of high oil price and global climate change. Most of ethanol is currently produced from corn grain or sugars in sugarcane and sugar beet. Because these sources compete with foods and animal feed and are not expected to be enough for future demand of ethanol. Thus, cellulosic ethanol from agricultural residues or wood has to be commercialized in near future. Typical cellulosic ethanol production consists of pretreatment, enzyme hydrolysis, fermentation and product separation. This paper reviews the principles and status of each step and discusses issues for cellulosic ethanol production.
Development of Bioartificial Skin for Skin Regeneration
Seo, Young-Kwon ; Song, Kye-Yong ; Park, Jung-Keug ;
KSBB Journal, volume 23, issue 1, 2008, Pages 8~17
There are many different approaches to healing of acute and chronic ulcer and large skin defect, such as burn. Currently available wound covers fall into two categories. Permanent covering, such as autografts, and temporary ones, such as allograft including de-epidermized cadaver skin, bioartificial skin, xenografts, and synthetic dressings. Autologous skin grafting in the form of split- or full-thickness skin is still the good standard. Following on from developments in the 1980s involving the use of cultured keratinocyte grafts in wound healing, the last decade has been great progress in the fabrication of composite bioartificial skin grafts. However, two bottleneck on producing cultured bioartificial skin, whether of the simple epithelial cell sheet type, or the more complex composite type, continue to be the generation of sufficient keratinocytes cheaply and quickly and develop biocompatible dermal scaffolds. This article covers the development, clinical application, and current research directions associated with bioartificial skin.
Exopolysaccharide (EPS) Production by Lactobacillus paracasei KLB58 in Modified Medium under Different Growth Conditions
Lee, Choong-Young ; Jeon, Jeong-Min ; Lee, Hae-In ; Kim, Min-Hee ; Jung, Mi-Kyoung ; So, Jae-Seong ;
KSBB Journal, volume 23, issue 1, 2008, Pages 18~22
Various probiotic Lactobacillus spp. are known to produce exopolysaccharide (EPS) which has potential health promoting functionality. A Lactobacillus paracasei strain producing EPS was isolated from healthy human. This strain, named L. paracasei KLB58, was grown on modified MRS medium. Experiments were conducted under various growth conditions to optimize the EPS production. Our study showed that incubation temperature played an important role in EPS production. When incubation temperature was changed from
, the increase of EPS production (28.1 mg/ml) was the highest in our experiment. The type of carbon source in the medium also affected EPS production. Galactose was the most effective for EPS production among the carbon sources examined. Using galactose, glucose, lactose and sucrose, the amount of released EPS was 38.9 mg/ml, 35.6 mg/ml, 21.76 mg/ml and 16.9 mg/ml, respectively. However, acidity in growth medium inhibited EPS productivity due to the low growth yield. When grown at pH 4, L. paracasei KLB58 could only produce EPS of 14.6 mg/ml. When the initial amounts of nitrogen and carbon sources were examined, EPS production was not significantly affected by nitrogen source while carbon source affected considerably.
Production and Characteristics of an Extracellular Pigment through the Submerged Cultivation of Phellinus Sp.
Lee, Dong-Ki ; Lee, Chul-Won ; Lee, Shin-Young ;
KSBB Journal, volume 23, issue 1, 2008, Pages 23~30
An extracellular pigment production of three Phellinus sp. (Phellinus 421, P. linteus and P. hartigil) through submerged cultivation was investigated. The maximum brown pigment from culture broth was obtained from the precipitate by addition of 10% 1M HCI solution. This precipitate showed absorption characteristics with
of 360nm. The maximum production of extracellular pigment obtained at optimum medium and culture condition was 3.54 (
). The precipitate was fractionated by Sephadex G-75 gel chromatography, and the isolated brown pigment contained a large amount of polyphenol and the small amounts of sugar and protein. The brown pigment fraction was stable in temperature range of
, pH range of
, sugar addition ranges of
and salt addition concentration of 3 molarity. Antioxidative activity of the brown pigment by TBA method was better than that of vitamin E (
Determination of Adsorption Isotherms and Separation of L-arabinose and D-ribose in Cation Exchange Chromatography and HPLC
Jeon, Young-Ju ; Kim, In-Ho ;
KSBB Journal, volume 23, issue 1, 2008, Pages 31~36
The use of L-carbohydrates and their corresponding nucleosides in medicinal application has greatly increased. For example L-ribose has been much in demand as the starting material for curing hepatitis B. High performance liquid chromatography (HPLC) method was studied for the analysis of ribose and arabinose fractions from ion exchange chromatography (IEC). Dowex Monosphere 99 Ca/320 resin was packed in IEC to separate ribose and arabinose under various operating conditions.
and sugar HPLC columns were then used to analyze the fractions from the IEC column. Pulse input method (PIM) was also used to measure adsorption isotherms of ribose and arabinose in the Dowex column and HPLC columns. Experimental results and simulations by ASPEN chromatography were compared with fair agreement.
Industrial-Scale Production of High-Purity Antihemophilic Factor IX from Human Plasma
Kang, Yong ; Choi, Yong-Woon ; Sung, Hark-Mo ; Sohn, Ki-Whan ; Shin, Jeong-Sup ; Kim, In-Seop ;
KSBB Journal, volume 23, issue 1, 2008, Pages 37~43
The use of antihemophilic factor IX complex has been associated with a variety of thrombotic complications, the major cause of which was the contamination of thrombogenic proteins such as vitamin K-dependent clotting factors II, VII, and X. In order to produce a commercial factor IX (GreenNine VF) free from thrombogenic potential, industrial-scale production process for high-purity factor IX from human plasma has been developed. The purification process contains cryo-precipitation, DEAE-sephadex A-50 anion-exchange chromatography, DEAE-toyopearl 650M anion-exchange column chromatography, heparin-sepharose 6FF affinity column chromatography, and CM-sepharose FF cation-exchange column chromatography. Also the process includes two viral inactivation and removal procedures, solvent/detergent treatment and nanofiltration using Viresolve NFP filter. The purification yield was 35.4%. The specific activity in the purified concentrate was 190.8 IU/mg which exceeded that in the factor IX complex (FacNine) by a factor of 48. The activities of factor II, VII, and X were not detected in GreenNine VF. SDS-PAGE analysis showed that GreenNine VF had the highest purity in comparison with commercially available high purity factor IX concentrates, Mononine, Octanyne, Berinin HS, and Immunine STIM plus 600. One batch size of the production was 2,400 vials of 250 IU product or 1,200 vials of 500 IU product from 1,600 L cryo-poor plasma.
NDP-sugar production and glycosylation of
-rhodomycinone in Streptomyces venezuelae
Park, Sung-Hee ; Cha, Min-Ho ; Kim, Eun-Jung ; Yoon, Yeo-Joon ; Sohng, Jae-Kyung ; Lee, Hee-Chan ; Liou, Kwang-Kyoung ; Kim, Byung-Gee ;
KSBB Journal, volume 23, issue 1, 2008, Pages 44~47
Anthracycline antibiotics doxorubicin (DXR) is clinically important cancer therapeutic agent produced by Streptomyces peucetius. DXR result by further metabolism of rhodomycin D (RHOD) and require a deoxy-sugar component for their biological activity. In this study, production of TDP-L-daunosamine and its attachment to
-rhodomycinone (RHO) to generate RHOD has been achieved by bioconversion in Streptomyces venezuelae that bears eleven genes. S. peucetius seven genes (dnmUTJVZQS) were transformed by plasmid and S. venezuelae two genes desIII, IV and two more S. peucetius drrA, B genes were integrated into chromosomal DNA. To generate the feeding substrate RHO, 6L S. peucetius grown on agar plate was harvested, extracted with organic solvent and then purified using preparative HPLC. Recombinant S. venezuelae grown on agar plate containing RHO was harvested and its n-butanol soluble components were extracted. The glycosylated product of aromatic polyketide RHO using heterologous host S. venezuelae presents the minimal information for TDP-L-daunosamine biosynthesis and its attachment onto aglycone. Moreover, the structure of auxiliary protein, DnrQ, was predicted by fold recognition and homology modeling in this study. This is a general approach to further expand of new glycosides of antitumor anthracycline antibiotics.
Biochemical Properties of Locular Fluid Lectin of Tomato
Roh, Kwang-Soo ;
KSBB Journal, volume 23, issue 1, 2008, Pages 48~53
Lectin was isolated from locular fluid of tomato by affinity chromatography using Sephadex G-200, and studied its some biochemical properties. SDS-PAGE of the isolated lectin revealed a tetramer composed of two identical subunits with molecular weights of 39 and 23 kDa. The isolated lectin was agglutinated by trypsin-treated human ABO type blood erythrocytes with similar potency, and the most activity of agglutination was found at B type blood erythrocyte. This lectin showed maximum thermal stability at
, and was relatively stable to heat with the higher activity at
. The optimal temperature and pH of this lectin were
and pH 7.0, respectively.
The Optimization of Biohydrogen Production Medium by Dark Fermentation with Enterobacter aerogenes
Kim, Kyu-Ho ; Choi, Young-Jin ; Kim, Eui-Yong ;
KSBB Journal, volume 23, issue 1, 2008, Pages 54~58
Hydrogen is considered as an energy source for the future due to its environmentally friendly use in fuel cells. A promising way is the biological production of hydrogen by fermentation. In this study, the optimization of medium conditions which maximize hydrogen production from Enterobacter aerogenes KCCM 40146 were determined. As a result, the maximum attainable cumulative volume of hydrogen was 431
under the conditions of 0.5M potassium phosphate buffer, pH 6.5 medium containing 30 g/L glucose. The best nitrogen sources were peptone and tryptone for the cell growth as well as hydrogen production. The control of cell growth rate was found to be a important experimental parameter for effective hydrogen production
Production of casein phosphopeptides using Streptococcus faecalis var. liquefaciens cell immobilization
Lee, Ki-Sung ; Shin, Jae-Yoon ; Jang, Yi-Hyun ; Kweon, Dae-Hyuk ; Park, Ki-Moon ; Jin, Yong-Su ;
KSBB Journal, volume 23, issue 1, 2008, Pages 59~64
Optimum conditions for production of casein phosphopeptides (CPP) from sodium casenate by immobilized cell culture of Streptococcus faecalis var. liquefaciens were investigated. Immobilized cells were made by mixing 60% sodium alginate solution with an equal volume of culture broth at the end of exponential phase and subsequently dropping the mixture into
solution. Optimum conditions for CPP production by the immobilized cells were the same as those (
, pH 7.0, and 10% substrate concentration) by the crude enzyme solution from the supernatant of culture broth. Optimum loading volume of the immobilized cells into a batch reactor was 30% (w/v). Using a continuous reactor loaded by the immobilized cells under the identified optimal conditions, we were able to produce CPP continuously up to 30 days with a maximum CPP conversion efficiency of 20%.
Characteristics of the Antibacterial Substance Produced by Paenibacillus polymyxa JB115
Jung, Hee-Kyoung ; Park, Seung-Chun ; Park, Byung-Kwon ; Kim, Sang-Dal ; Nam, Doo-Hyun ; Hong, Joo-Heon ;
KSBB Journal, volume 23, issue 1, 2008, Pages 65~69
The culture broth of a
-glucan-producing bacterium, Paenibacillus polymyxa JB115, was confirmed to show the antibiosis against pathogenic bacteria of livestock disease. The antibacterial substance produced by P. polymyxa JB115 exhibited strong bactericidal or bacteriostatic effect on the growth of livestock pathogenic bacteria including Staphylococcus aureus, Salmonella choleraesuis, Escherichia coli and Pseudomonas aeruginosa. This antibacterial substance also showed high stabilities in broad pH range (pH 3-11) and in broad temperature range
, which is good enough to apply spray-dry method for the formulation of culture broth. It was also found that the antibacterial substance was very stable in artificial gastric fluid and bile acid, which implies the anticipated antibacterial activity against gastrointestinal bacteria harmful for livestocks. In conclusion, the culture broth of P. polymyxa JB115 can be developed as a multifuctional feed additive containing immune-enhancing
-glucan as well as antibacterial agent against livestock pathogenic bacteria.
The Removal of Organics and Nutrients in an Anoxic/Oxic Process Using Surface-modified Media
Seon, Yong-Ho ;
KSBB Journal, volume 23, issue 1, 2008, Pages 70~76
Surface of hydrophobic media was modified to become hydrophilic by ion beam irradiation. Fixed bed biofilm reactors packed with or without surface modification were used to remove organics, nitrogen, and phosphorus from sewage. This system composed of anoxic/oxic cycles to increase the nutrient removal. A cylindrical polyethylene was used as a packing media in this study. With 12 hours of hydraulic retention time (HRT), the reactors with and without surface modification showed 95% and 92%
removal, respectively. Both reactors showed over 95%
removals for a longer HRT of 16 hours. Nitrogen removal ranged 54.8% to 70.2% for the surface modified system and 57.5% to 76.5% for the non-modified system under same condition. Finally, phosphorus removal ranged 59.4% to 69.8% for the surface modified system and 51.3% to 63.4% for the non-modified system under same condition. From this study organics and phosphorus were better removed in using surface modified media and vice versa for nitrogen removal.
Antioxidant Effects and Melanin Inhibitory Effect of Natural Pimpinella komarovii Extracts in Jeju Island
Kang, Min-Chul ; Lee, Ju-Yeop ; Lee, Jung-A ; Han, Jong-Heon ; Kim, Bong-Seok ; Kim, Gi-Ok ;
KSBB Journal, volume 23, issue 1, 2008, Pages 77~82
We investigated several biological activities using the ethanol extract and its fractions from Pimpinella komarovii leaves to evaluate the usefulness of its extract as a functional biomaterial. The ethanol extract showed antioxidant activities, such as DPPH scavenging activity
. superoxide scavenging activity
, and xanthine oxidase inhibitory activity
. Its EtOAc fraction showed the strongest antioxidant activities among several fractions. The inhibitory effect of ethanol extract on tyrosinase activity was higher than water fraction. When
of EtOAc fraction was applied, the inhibition ratio of tyrosinase activity was much higher (42%) than that of melasolv. The EtOAc fraction also showed higher inhibitory effect on melanogenesis in Melan-a cells. The n-hexane and EtOAc fractions dose-dependently inhibited the NO production in a RAW 264.7 cells. These results suggest that extract of Pimpinella komarovii could be used as functional biomaterial in developing a skin whitening agent having the antioxidant activity.
Treatment of highly concentrated organic wastewater by high efficiency
Kim, Jung-Kon ; Jung, Hyo-Ki ; Son, Joo-Young ; Kim, Si-Wouk ;
KSBB Journal, volume 23, issue 1, 2008, Pages 83~89
Food wastewater derived from the three-stage methane fermentation system developed in this lab contained high concentration organic substances. The organic wastewater should be treated through advanced wastewater treatment system to satisfy the "Permissible Pollutant Discharge Standard of Korea". In order to treat the organic wastewater efficiently, several optimum operation conditions of a modified
photocatalytic system have been investigated. In the first process, wastewater was pre-treated with
. The optimum pH and coagulant concentration were 4.0 and 2000mg/L, respectively. Through this process, 52.6% of CODcr was removed. The second process was
photocatalytic reaction. The optimum operation conditions for the system were as follows: UV lamp wavelength, 254 nm; wastewater temperature,
; pH 8.0; and air flow rate, 40L/min, respectively. Through the above two combined processes, 69.7% of T-N and 70.9% of CODcr contained in the wastewater were removed.
Performance Evaluation of Bio-Membrane Hybrid Process for Treatment of Food Waste Leachate
Lee, Myung-Gu ; Park, Chul-Hwan ; Lee, Do-Hoon ; Kim, Tak-Hyun ; Lee, Byung-Hwan ; Lee, Jin-Won ; Kim, Sang-Yong ;
KSBB Journal, volume 23, issue 1, 2008, Pages 90~95
In this study, a combined process of sequential anaerobic-aerobic digestion (SAAD), fluidized-bed bioreactor (FBBR), and ultrafiltration (UF) for the treatment of small scale food waste leachate was developed and evaluated. The SAAD process was tested for performance and stability by subjecting leachate from food waste to a two-phase anaerobic digestion. The main process used FBBR composed of aerators for oxygen supply and fluidization, three 5 ton reaction chambers containing an aerobic mesophilic microorganism immobilized in PE (polyethylene), and a sedimentation chamber. The HRTs (hydraulic retention time) of the combined SAAD-FBBR-UF process were 30, 7, and 1 day, and the operation temperature was set to the optimal one for microbial growth. The pilot process maintained its performance even when the CODcr of input leachate fluctuated largely. During the operation, average CODcr, TKN, TP, and salt of the effluent were 1,207mg/L, 100mg/L, 50 mg/L, and 0.01 %, which corresponded to the removal efficiencies of 99.4%, 98.6%, 89.6%, and 98.5%, respectively. These results show that the developed process is able to manage high concentration leachate from food waste and remove CODcr, TKN, TP, and salt effectively.
Antioxidant activity analysis of Catechin compounds in Korean green tea using HPLC On-line
Antioxidant screening system
Lee, Kwang-Jin ;
KSBB Journal, volume 23, issue 1, 2008, Pages 96~100
In this work, we describes analysis of the antioxidant potential of Korean green tea phenolics using an high-performance liquid chromatography (HPLC) on-line
antioxidant screening method. In conjunction with the analysis of their 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) radical scavenging ability, the extraction of catechine compounds from Korean green tea were performed by various temperature and time. The optimum operating conditions were experimentally determined to analyze the catechine compounds in the pretreatment extracts. From the results, the extraction temperature
, time 3 min was selected as an optimal antioxidant activity condition. The analysis by
column was performed, the flow rate of mobile phase and UV wavelength was fixed at 1.0 ml/min and 254 nm, respectively. the mobile phase was composed from acetonitrile and water, and the gradient elution mode were applied.