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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 23, Issue 6 - Dec 2008
Volume 23, Issue 5 - Oct 2008
Volume 23, Issue 4 - Aug 2008
Volume 23, Issue 3 - Jun 2008
Volume 23, Issue 2 - Apr 2008
Volume 23, Issue 1 - Feb 2008
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Extraction of Useful component from Natural plants using Ultrasound system
Lee, Kwang-Jin ; Um, Byung-Hun ;
KSBB Journal, volume 23, issue 2, 2008, Pages 101~108
Ultrasound assisted extraction (UAE) process enhancement for food and allied industries are reported in this review. Recently ultrasound system has been used more frequently to extract drugs and natural substances. This modern emulsification, homogenization, particle size reduction and viscosity alteration methodologies require reliable tools that perform on a high level in terms of efficiency and reproducibility. Also, Ultrasound can enhance existing extraction processes and enable new commercial extraction opportunities and processes. Therefore, ultrasound technologies could have a strong presence in the future of the food industry.
Phellinus linteus; Market and Technology Trends Analysis
Sohn, Eun-Hwa ; Roh, Hyun-Sook ; Park, Young-Seo ; Sohn, Eun-Soo ; Kang, Se-Chan ; Kang, Nam-Sung ; Pyo, Suhk-Neung ;
KSBB Journal, volume 23, issue 2, 2008, Pages 109~117
Phellinus linteus (Sang Hwang mushroom; P. linteusis) has been widely used for a traditional medicine in Japan, Korea and China. In traditional oriental applications, P. linteus and their extracts have been exclusively used to treat various diseases like insomnia, neurasthenia, gastric ulcers, arthritis, nephritis, stress, asthma, bronchitis, neuromuscular disorders, hypertension and also as an immune system stimulator in cancer therapy. In a number of articles, it is indicated how tremendous their therapeutic values are. According to these studies, P. linteus and their extracts show immunomodulatory activities like enhancing immune system function including anti-tumor activity and inhibiting the growth of existing tumors. P. linteus and their extracts have been being studied for use as immunotherapy agents and biological response modifiers (BRMs) for the possible treatment of cancer without side effects. Recently, the cultured mycelium product of P. linteus has been developed and approved as a medicine in Korea. This review shows not only the current status of methods of developmental technology like artificial culture and various biological applications of P. linteus but also recent market trends of functional foods made from P. linteus.
Modulation of Bacteria with a Combination of Natural Products in KIMCHI Fermentation
Kim, Jong-Deog ; Shin, Jin-Hyuk ; Seo, Hyo-Jin ; Lim, Dong-Jung ; Hong, Soon-Kang ; Shin, Tai-Sun ;
KSBB Journal, volume 23, issue 2, 2008, Pages 118~124
Three kinds of bacteria that influence Kimchi fermentation, Lactobacillus plantarium for acidity, Leuconostoc mesenteroides for ripening Kimchi, and Pichia membranifaciens for decreasing Kimchi quality, were regulated by natural products including Theae folium, Taraxacum coreanum, Brassica juncea, Astragali radix, Gynostemma pentaphyllum, Camellia japonica, Agaricus blazei, and Cordyceps militaris. The common prescription combined T. folium, T. coreanum and C. militaris and simultaneously regulated these 3 bacteria as follows: the growth of L. plantarium and P. membranifaciens were inhibited and L. mesenteroides was promoted. The most effective mixing ratio was T. folium: T. coreanum: C. militaris = 3:2:1. With this new prescription, deep flavor, extended preservation, and a special taste are expected in the Kimchi due to these natural products.
Biological activities of ethanol extract from the seawater algae, Chlorella elliposidea C020
Kim, Hyun-Jin ; Kim, In-Hae ; Lee, Jae-Hwa ;
KSBB Journal, volume 23, issue 2, 2008, Pages 125~130
We investigated the biological activities of ethanol extract from the seawater algae, Chlorella elliposidea C020 such as antibacterial activity, anti-oxidant activity, tyrosinase inhibitory activity and hemolytic activity against human erythrocytes. Extract was obtained from various solvent, methanol, ethanol, acetone and ethanol + acetone (1:1, v/v%), 95% ethanol proved to be best extraction solvents. The contents of ethanol extract were higher in freeze-dried sample than that in frozen-thawing. Antibacterial activities of ethanol extract showed strong inhibitory effect against Bacillus subtilis PM125, Bacillus licheniformis and fish pathogenic bacteria, Vibrio parahaemolyticus KCTC2471 and Edward tarda NUF251. However, this extract didn't worked against antifungal activity against Candida albicans KCTC1940. And, ethanol extract was without hemolytic activity against human erythorocytes. The ethanol extract showed 75% of free radical scavanging effect on 2.0 mg/mL using DPPH method. In tyrosinase inhibition assay of ethanol extract,
(Inhibition Concentration) was measured as 10.87 mg/mL. Conclusionally, ethanol extract of Chlorella elliposidea C020 has good candidate for bioactive materials.
Production of kaempferol by enzymatic hydrolysis of tea seed extract
Lim, Yun-Young ; Kim, Eun-Ki ;
KSBB Journal, volume 23, issue 2, 2008, Pages 131~134
Tea seed extract, a byproduct of tea processing, contains two kaempferol glycosides, camelliaside A and camelliaside B. Kaempferol was produced by enzymatic hydrolysis of glycosides. Optimum reaction conditions were investigated.
was effective, producing kaempferol in 48 hrs. Optimum temperature and pH were
and 4, respectively. Ratio of substrate and enzyme affected the yield. Under optimum conditions, 1.6g kaempferol per 1 kg tea seed extract was produced and 80% of kaempferol precipitated. This result shows that kaempferol could be produced mildly and effectively using tea-processing byproduct.
Simulation of SMB [Simulated Moving Bed] Chromatography for Separation of L-ribose and L-arabinose by ASPEN chromatography
Lee, Seon-Hee ; Lee, Eun ; Kim, In-Ho ;
KSBB Journal, volume 23, issue 2, 2008, Pages 135~141
SMB (simulated moving bed) chromatography is a very useful utility for the separation of binary system. We simulated the separation of L-arabinose and L-ribose from the mixture by using lab-scale 4(1-1-1-1)-zone SMB chromatography. Preliminary experiments of PIM (pulse input method) were performed to measure adsorption isotherms of L-ribose and L-arabinose in
HPLC column, and experimental and simulated results from ASPEN chromatography were compared. To find the most suitable separation condition in SMB, we carried out a simulation in
plane base on the triangle theory and calculated operating parameters (flow rate of four zone, switching time and feed concentration and so on) using ASPEN chromatography under the conditions of linear isotherms obtained from PIM.
Effect of Mulberry Extracts on Secretion of MMPs and Plasmin in U-373-MG Cells
Lee, Suk-Hee ; Kim, Hwan-Gyu ;
KSBB Journal, volume 23, issue 2, 2008, Pages 142~146
In order to sprout and migrate, cells must secrete proteinases which are degrading the surrounding extracellular matrix. In this study, we examined the effect of mulberry extracts and combination of mulberry extracts and VEGF on human malignant glioma U-373-MG cells. Mulberry extracts induced the secretion of matrix metalloproteinase-9 (MMP-9) and suppressed the secretion of MMP-2 and plasmin. Mulberry extracts inhibited the VEGF-induced MMP-2, MMP-9 and plasmin secretion. It is therefore, suggested that mulberry extracts can suppress the VEGF-induced tumor angiogenesis in U-373-MG cells. Also, mulberry extracts induced the secretion of MMP-9 and plasmin through PI 3'-kinase pathway in U-373-MG cells.
Antimicrobial Activity of Extracted by Supercritical Fluid from Origanum vulgare, Cinnamomum cassia, Chamaecyparis obtusa and Scutellariae baicalensis
Kim, Woo-Jin ; Cho, Jun-Young ; Choi, Chang-Suk ; Yoon, Gee-Sun ; Lee, Won-Kyu ; Ryu, Yeon-Woo ;
KSBB Journal, volume 23, issue 2, 2008, Pages 147~152
The variety of functional plants has an attention for new natural food preservation and natural antiseptic development. The extracts from functional plants with various methods (ethanol extraction, hot water extraction and supercritical fluid extraction) tested antimicrobial activity against 10 strains including the pathogenic and food poisoning bacteria, the yeast and fungi. The antimicrobial activities of supercritical fluid extracts were shown higher than ethanol extract and hot water extract when tested with disc-diffusion method and minimum inhibitory concentration (MIC). Antimicrobial activity of supercritical fluid extract was two times higher than ethanol extract in Cinnaonomum cassia. In addition, the supercritical fluid extractions of Chamaecyparis obtuas and the C. cassia showed the higher yield than Origanum vulgare and Scutellariae baicalensis. The supercritical fluid extract of C. cassia showed an antimicrobial activity against all strains tested. The supercritical fluid extract of S. baicalensis showed strong antimicrobial activity on Listeria monocytogenes. Supercritical fluid extraction of O. vulgare and C. obtuas showed strong antimicrobial activity on Salmonella typhimuriium. In MIC test, C. obtuas was shown the best natural material for the preparation of natural antimicrobial agent by supercritical fluid extraction. In conclusion, these results suggest that supercritical fluid extraction technique was effective to obtain functional ingredient with higher antimicrobial activity in the development of new antimicrobial reagent from natural materials.
Isolation and Identification of Macamides from the Lipidic Extract of Maca [Lepidium meyenii] using Supercritical Carbon Dioxide
Lee, Seung-Ho ; Kang, Jung-Il ; Lee, Sang-Yun ; Ha, Hyo-Cheol ; Song, Young-Keun ; Byun, Sang-Yo ;
KSBB Journal, volume 23, issue 2, 2008, Pages 153~157
Maca (Lepidium meyenii) has been used as a food and medicine in Peru for thousands of years. More recently a wide array of commercial maca products have gained popularity as dietary supplements with claims of anabolic and aphrodisiac effects. Even though the biologically active principles of maca are not fully known, the lipidic extract of maca tubers containing macamides showed promising physiological activities. In this study, the lipidic extract were collected from maca tubers by using supercritical carbon dioxide (
). Substance estimated as macamide in the extract was isolated and purified by preparative HPLC with recycling system. Two of the purified substance was identified as N-benzyl-5-oxo-6E,8E-octadecadienamide and N-benzylhexadecan amide by LC/MS,
Antibacterial, antioxidant and antitumor activities of mushroom mycelium mixed culture extracts
Kim, Man-Cheol ; Kim, Ju-Sang ; Heo, Moon-Soo ;
KSBB Journal, volume 23, issue 2, 2008, Pages 158~163
In this study, we have investigated the antibacterial, antioxidant, and antitumor activities of mycelium cultural extract from mushroom. Mushroom mycelium was grown in a synthetic liquid media such as PD broth, YM broth or citrus extracts. In antibacterial activity test, the best result was achieved when mycelium cultural extracts from Phellinus linteus and Coriolus versicolor were incubated together on YM broth. On the other hand, mushroom mycelium cultured on citrus extracts showed better activity than that on PD broth. We have also tested the antioxidant activity at concentration up to 10 mg of mycelium cultural extract/mL. The more it is in higher concentration, the more the activity increases. The higher antioxidant activity was observed both on PD broth containing the Phellinus linteus and Coriolus versicolor mycelium and citrus extract containing the same. The complex culture extracts obtained from the synthetic medium and citrus extract medium showed 10-89% of the 1,1-diphenyl-2-picrylhydrazyl radical scavenger activity. The antitumor activity of mycelium cultural extract was examined by using MTT assay on A549 cells. Mushroom mycelium cultured on citrus extracts showed interestingly higher antitumor activity than that on synthetic liquid media.
Physiological Activities of Extracts of Cedrela sinensis leaves
Shin, Hee-June ; Jeon, Young-Jin ; Shin, Hyun-Jae ;
KSBB Journal, volume 23, issue 2, 2008, Pages 164~168
The purpose of this study was to confirm the content of total polyphenol, antioxidative and immune activities of the extracts of Cedrela sinensis leaf. The content of total polyphenol of water extracts ranged from 46.5-59.6 mg/100 g, which was higher than other extracts using organic solvents such as EtOAc,
. The antioxidant activity of the water and organic solvents extracts showed 6-33% in terms of 2,2-diphenyl-picryl-hydrazyl (DPPH) scavenging activity. To analyze the immuno-stimulation activity of C. sinensis leaf extract, we investigated the effect of the extracts on NO synthesis which is important in host defense against bacterial infection. Hot water extracts significantly increased NO generation by RAW 264.7, macrophage cell line, while organic solvent extract has no significant effect on NO production. To further analyzed the anti-inflammatory effect of the extracts, we investigated the effects of the extracts on lipopolysaccharide(LPS)-induced NO generation. Organic solvent extracts of C. sinensis leaves showed strong inhibitory effect on NO production in LPS-stimulated RAW 264.7 cells. These results suggest that C. sinensis leaf extract may represent a useful immune stimulating agent and anti-inflammatory agent.
Concentration of Fresh Gel from Aloe vera L. by Using Ultrafiltration Process
Baek, Jin-Hong ; Kim, Sung-A ; Lee, Shin-Young ;
KSBB Journal, volume 23, issue 2, 2008, Pages 169~176
The concentration of fresh gel from Aloe vera L. by using ulfrafiltration (UF) process was investigated and analyzed. The two membranes (organic and ceramic) with different molecular weight cut-off (MWCO) and modules (flat sheet and tubular) was used. Under optimum operation conditions, ceramic (zirconium dioxide) tubular membrane with MWCO of 50 kDa resulted in higher flux, less fouling, more turbid, higher total solid, higher polysaccharide and less aloin content. Optimum operation conditions were transmembrane pressure of 1.0 bar, feed velocity of 240 L/hr and temperature of
. Volume concentration factor of aloe gel was 3.13 at permeate flux of
after processing time of 1.66 hr. Aloin in fresh aloe gel by UF process was effectively removed as permeate and bioactive polysaccharide content was 2.1 times higher than that of fresh aloe gel. These results allowed a very good level of concentration degree and polysaccharide content. Thus, ultrafiltration process of this study was suitable for the concentration of fresh aloe gel though the aloe concentrate showed both the viscosity decrease and partially separation of liquid layer during storage at
Inhibition effects of extracts and its solvent fractions isolated from Limonium tetragonum on growth of human cancer cells
Kong, Chang-Suk ; Um, Young-Ran ; Lee, Jung-Im ; Kim, You-Ah ; Lee, Jeom-Sook ; Seo, Young-Wan ;
KSBB Journal, volume 23, issue 2, 2008, Pages 177~182
In this study, crude extracts of the halophyte Limonium tetragonum and their solvent fractions were evaluated on anticancer activity in AGS and HT-29 human cancer cells using MTT assay. Each of the crude extracts (MeOH and
) of Limonium tetragonum showed a significant inhibitory effect on the growth of human cancer cells. The combined crude extracts of MeOH and
were partitioned between
and water. The organic layer was further partitioned between 85% aq. MeOH and n-hexane, and then the aqueous layer was fractionated with n-BuOH and
, successively. Growth inhibition effects of crude extracts and their solvent fractions from Limonium tetragonum increased in a dose-dependent manner. Among them, 85% aq. MeOH, n-hexane and n-BuOH fractions revealed very good inhibition effects on the growth of human cancer cells. These results suggest that we can isolate active compounds from Limonium tetragonum to show much more strong anticancer activity.
Removal of Residual Toxic Phytohormone in Suspension Cultures of Panax vietnamensis
Lee, Seung-Ho ; Kim, Nam-Hyuck ; Kim, Ki-Hyun ; Quang, Le Bach ; Luong, Hoang Van ; Byun, Sang-Yo ;
KSBB Journal, volume 23, issue 2, 2008, Pages 183~185
Studies were made to remove the toxic phytohormone, 2,4-D, in suspension cultures of Panax vietnamensis. Cells grown in normal MS medium with 2,4-D were inoculated and grown in the MS medium without hormone. Not a big difference was observed in growth characteristics between media with and without 2,4-D. The 2,4-D in the culture, however, was completely removed. During the culture, the residual 2,4-D was consumed rapidly at the early growth stage. The intra-cellular 2,4-D was consumed first and the 2,4-D in the medium was used afterward.