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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 23, Issue 6 - Dec 2008
Volume 23, Issue 5 - Oct 2008
Volume 23, Issue 4 - Aug 2008
Volume 23, Issue 3 - Jun 2008
Volume 23, Issue 2 - Apr 2008
Volume 23, Issue 1 - Feb 2008
Selecting the target year
Isolation of lysozyme producing bacteria capable of solubilizing microbial cells
Guo, Pengfei ; Seo, Sun-Keun ; Zhang, Lei ; Kim, Hyo-Sang ; Oh, Young-Khee ; Jahng, Deok-Jin ;
KSBB Journal, volume 23, issue 3, 2008, Pages 187~192
Lysozyme-producing microorganisms were isolated to obtain bacteria which can efficiently solubilize microbial cells. Cells of normal and chloroform-treated Escherichia coli and Micrococcus Iysodeikticus were used as model substrates to isolate lysozyme-producing microorganisms and investigate the efficiency of cell lysis. The culture supernatant of the isolate New1 (98% similarity of 16S rDNA sequence with Thermomonas haemolytica) showed different lytic characteristics for different substrates. Thermal treatment (autoclave) of substrate cells showed a significant effect on cell solubilization by culture supernatant of the New1. For autoclaved substrate cells, E. coli, M. Iysodeikticus and chloroform-treated E. coli were solubilized by 58.7%, 49.4% and 79.1%, respectively, in the culture supernatant of New1. The lytic activity of New1 was mainly caused by lysozyme produced by the isolate. It was also showed that New1 exhibited high protease activity and a little cellulase activity.
Enrichment of Coffee Flavors with Supercritical Carbon Dioxide
Lee, Joo-Hee ; Byun, Sang-Yo ;
KSBB Journal, volume 23, issue 3, 2008, Pages 193~198
In this study, the supercritical
extraction was applied for the enrichment of coffee flavors. The extraction efficiency of coffee flavors was dependent on the pressure and temperature and optimized as 350 bar and 80
C. Five flavors of high aroma values were analyzed. The flavors extracted by the supercritical
extraction were 76.6 times higher than those by the traditional method, espresso extraction. The modified headspace system proved the enhanced efficiency of supercritical
extraction. The coffee beverage containing the extract of supercritical
resulted 2.3 times of flavor enrichment when it was compared to that without the supercritical extract.
Hydrogen Production from Wastewater in Takju Manufacturing Factory by Microbial Consortium
Lee, Ki-Seok ; Bae, Sang-Ok ; Kang, Chang-Min ; Chung, Seon-Yong ;
KSBB Journal, volume 23, issue 3, 2008, Pages 199~204
Culture conditions for biological hydrogen production were investigated in wastewater of Takju manufacturing factory. Rhodobacter spaeroides KCTC1425, photosynthesis bacteria, and Enterobacter cloacae YJ-1, anaerobic bacteria were used. The hydrogen production were
broth for Rhodobacter spaeroides KCTC1425 and
broth for Enterobacter cloacae YJ-1 during 36 h. The hydrogen production increased with light intensity, and were highest over 12000Lux. In mixed culture of Rhodobacter spaeroides KCTC1425 and Enterobacter cloacae Y J-1, the optimum mixing ratio of hydrogen production was 20 and 80. Adding volume of yeast extract for maximum hydrogen production was 15
, but there was no effect over that.
was most effective among the inorganic salts, and the optimum volume was 0.4
. In semi-continuous culture, total hydrogen production was
broth for 144 h with operating period of 24 h.
Study on the soluble exoression of recombinant human eoidermal growth factor using various fusion oartners in Escherichia coli
Kim, Byung-Lip ; Baek, Jung-Eun ; Kim, Chun-Sug ; Lee, Hyeok-Weon ; Ahn, Jung-Oh ; Lee, Hong-Weon ; Jung, Joon-Ki ; Lee, Eun-Gyo ; Kim, In-Ho ;
KSBB Journal, volume 23, issue 3, 2008, Pages 205~212
The efficient soluble expression of human epidermal growth factor (hEGF) was achieved by using functional fusion partners in cytoplasm and periplasm of Escherichia coli (E. coli). hEGF was over-expressed in inactive inclusion body form in cytoplasm of E. coli due to improper disulfide bond formation and hydrophobic interaction, yielding about 5.9 mg/L in flask culture. Six functional fusion partners were introduced by linking to N-terminal part of hEGF gene for the high-level expression of soluble and active hEGF in cytoplasm and peri plasm region. Three fusion partners for cytoplasmic expression such as acidic tail of synuclein (ATS), thioredoxin (Trx) and lipase, and three fusion partners for periplasmic expression such as periplasmic cystein oxidoreductases (DsbA and DsbC) and maltose binding protein (MBP) were investigated. hEGF fused with ATS and DsbA showed over 90% of solubility in cytoplasm and periplasm, respectively. Especially DsbA was found to be an efficient fusion partner for soluble and high-level expression of hEGF, yielding about 18.1 mg/L and three-fold higher level compared to that of insoluble non-fusion hEGF in cytoplasm. Thus, heterologous proteins containing complex disulfide bond and many hydrophobic amino acids can effectively be produced as an active form in E. coli by introducing a suitable peptide or protein.
Study of Continuous Production of Alcohol using Biologically Sandwich-styled Immobilization Carrier
Park, Young-G. ; Kim, Hee-Jung ;
KSBB Journal, volume 23, issue 3, 2008, Pages 213~218
The present study was to investigate the continuous production of alcohol using immobilized carrier manufactured by polymeric materials. Fermentation runs with a crushed rice, with constituents recovered from batch culture and with ones from continuous culture were thus compared. The performances of immobilized carrier were governed by sandwitched synthetic polymers, the evolution of the continuous culture was steadily governed by the production of alcohol in the lag time of batch culture. The main focus was set on the enhancement of the alcohol production by an newly-developed polymeric forms. This polymeric form led to a drastic increase of the microorganism and the production cost in the continuous reactor was thereby reduced. The sandwitched polymeric-formed carrier, which was resistant to external environments, serves as an interesting alternative to maintain the stability of biological process. These whole results were discussed with the aim to better understand the continuous process implied in the microorganism's build-up during cultivation of fermentation broth.
Isolation and Characterization of Microorganisms with Broad Antifungal Activity against Phytopathogenic Fungi
Kim, Min-Hee ; Ko, Hee-Sun ; Yook, Young-Min ; Kim, Hyun-Soo ;
KSBB Journal, volume 23, issue 3, 2008, Pages 219~225
For the production of an antifungal compound, one strain (I-8) was selected from approximately 400 strains isolated from various soil samples. The optimum carbon source, nitrogen source and pH culture conditions for the production of the antifungal compound were investigated. ISP No. 2 medium (yeast extract 0.4%, malt extract 1% and dextrose 0.4%, at pH 8) was determined to be the optimum medium. Strain I-8 showed broad antifungal activity against the plant pathogenic fungi tested, including Sclerotinia sclerotiorum KACC 41065, as well as cellulase and chitinase activities in an agar plate assay. The extraction of antifungal compounds was performed using ethyl ether and ethyl acetate. In a culture broth of strain I-8, the ethyl acetate extract exhibited effective growth inhibition against 14 of the 20 phytopathogenic fungi tested. By mixing the ethyl acetate extract from I-8 with the ethyl ether extract from the fungus 13-16, which shows specific antifungal activity against Colletotrichum orbiculare KACC 40808, the antifungal activity of I-8 against phytopathogenic fungi was confirmed to be slightly increased. Strain I-8 showed strong growth inhibition against 16 phytopathogenic strains in agar plate tests.
Adhesion properties of Microorganisms onto surfaces of phosphorylcholine(PC)-modified copolymer for sensor applications
Kim, Sun-Yong ; Sohn, Ok-Jae ; Chae, Kyu-Ho ; Rhee, Jong-II ;
KSBB Journal, volume 23, issue 3, 2008, Pages 226~230
In this study we have studied adhesive properties of various microorganisms onto surfaces of phosphorylcholine-based copolymer for the application of optical biosensors. Three microorganisms, E.coli JM109, B.cereus 318, P.pastoris X-33 were cultivated in confocal cultivation dishes with glass surface, respectively. The glass surface was coated with copolymer containing 0% 5% and 10% MPC (2-methacryloxyethyl phosphorylcholine). After cultivation, culture medium was discarded and adhered microorganisms were dyed by gram staining method. Adhered microorganisms were analyzed using an optical microscope and scanning electronic microscope (SEM). A great number of microorganisms,
were adhered on the surfaces of glass and copolymer membrane without MPC. But the antifouling effects of copolymer containing 5% and 10% phosphorylcholine were large, that microorganisms of less than
were attached on the copolymer membranes. Thus, the copolymer containing phosphorylcholine is very useful as an antifouling coating material for optical biosensor.
Enhancement of the solubility of human tissue inhibitor of matrix metallocroteinase-2 (TIMP-2) in E. coli using a modified in vitro mutagenesis
Kim, Jong-Uk ; Choi, Dong-Soon ; Joo, Hyun ; Min, Churl-K. ;
KSBB Journal, volume 23, issue 3, 2008, Pages 231~238
The second family member of tissue inhibitors of matrix metalloproteinases, TIMP-2, is a 21kDa protein which inhibits matrix metalloproteinases 2 (MMP-2). Expression of mammalian proteins in E. coli often forms inclusion bodies that are made up of mis-folded or insoluble protein aggregates. The requirement for the formation of 6 disulfide bonds in the process of the TIMP-2 folding is likely to be incompatible with the reducing environment of E. coli. However, this incompatibility can be often overcome by introducing a mutagenesis that could lead to enhancement of the protein solubility. In this reason, we have attempted to express the soluble TIMP-2 in E. coli by applying a modified staggered extension process (StEP), one of the in vitro PCR-based recombinant mutagenesis methods, and error-prone PCR. C-terminally located CAT fusion protein with respect to mutated TIMP-2 proteins enables us to differentiate the soluble TIMP-2 from the insoluble in E. coli by virtue of chloramphenicol resistance. According to this scheme, E. coli harboring properly-folded CAT fused to TIMP-2 protein was selected, and some of the resulting colonies exhibited an enhanced, soluble expression of TIMP-2 compared to the wild type, implying (i) the StEP technique is successfully employed to enhance the proper folding thereby increasing the solubility of TIMP-2, and (ii) the CAT dependent screening may be a simple and effective method to differentiate the soluble protein expression in E. coli.
Urease Inhibition and Flocculating Activity of Concentrated Aloe vera Gel by Using Ultrafiltration Process
Baek, Jin-Hong ; Kim, Sung-A ; Lee, Shin-Young ;
KSBB Journal, volume 23, issue 3, 2008, Pages 239~244
For physiological function of aloe concentrate by ultrafiltration (UF) process, jack bean urease inhibitory activity and bentonite flocculating activity of UF aloe concentrate was investigated and compared with fresh aloe gel. Urease inhibitory activity of UF aloe concentrate ranged from 87 to 90% in 1 mL sample. Also, urease inhibitory activity of UF aloe concentrate increased about 10% by heat treatment showing the heat stability. From Lineweaver-Burk plot for UF aloe concentrate, urease inhibition pattern indicated general non-competitive inhibition. From flocculation test of UF aloe concentrate about 1% (w/v) bentonite suspension, maximum flocclulating activity of 97% was obtained at 0.5 mL addition of UF aloe concentrate/ 5 ml bentonite suspension. However, flocculating activity of 81% was obtained at 1 mL addition of UF aloe concentrate/ 5 mL bentonite suspension, which was typical flocculating behavior of polymers with re-dispersion at overdose area. FT-IR spectra of UF aloe concentrate showed the characteristic patterns of
-binding polysaccharide and less deacetylation indicating higher level of bioactive polysaccharide content.
Integrated Wet Oxidation and Aerobic Biological Treatment of the Quinoline Wastewater
Kwon, S.S. ; Moon, H.M. ; Lee, Y.H. ; Yu, Yong-Ho ; Yoon, Wang-Lai ; Suh, Il-Soon ;
KSBB Journal, volume 23, issue 3, 2008, Pages 245~250
The treatment of a model wastewater containing quinoline in an integrated wet oxidation-aerobic biological treatment was investigated. Partial wet oxidation under mild operating conditions was capable of converting the original quinoline to biodegradable organic acids such as nicotinic, formic and acetic acid, the solution of which was subjected to the subsequent aerobic biological treatment. The wet oxidation was carried out at 250
and the initial pH of 7.0, and led to effluents of which nicotinic acid was oxidized through 6-hydroxynicotinic acid by a Bacillus species in the subsequent aerobic biological treatment. Either homogeneous catalyst of
or phenol, which is more degradable in the wet oxidation compared to quinoline, was also used for increasing the oxidation rate in the wet oxidation of quinoline at 200
. The oxidation of quinoline in the catalytic wet oxidation and the wet co-oxidation with phenol resulted in effluents of which nicotinic acid was biodegradable earlier in the aerobic biological treatment compared to those out of the non-catalytic wet oxidation at 250
. However, the lag phase in the biodegradation of nicotinic acid formed out of the wet oxidation at 250
was considerably shortened after the adaptation of Bacillus species used in the aerobic biological treatment with the effluents of the quinoline wet oxidation.
Fibrinolytic Activity and Characterization of Bacillus licheniformis HK-12 Isolated from Chungkook-Jang
Sohn, Byung-Hee ; Song, Yu-Jin ; Oh, Kye-Heon ;
KSBB Journal, volume 23, issue 3, 2008, Pages 251~256
The aim of this work was to investigate the fibrinolytic activity and characterization of Bacillus licheniformis HK-12, which produces the fibrinolytic enzyme excreted from naturally fermented Chungkook-Jang. Initially, the physiological and biochemical characteristics of strain HK-12 was examined. Both physiological analysis using BIOLOG system and phylogenetic analysis using 16S rRNA sequencing were performed to identify the strain, and the strain could be assigned to Bacillus licheniformis, designated as B. lichenformis HK-12, and registered in GenBank as [EU288193]. Phylogenetic analysis of B. licheniformis HK-12 was plotted based on 16S rRNA sequence comparisons. During the incubation period of B. licheniformis HK-12, the changes of bacterial growth, fibrinolytic activity, and pH were monitored. As the results, after 36 hours of incubation, the maximum fibinolytic activity was about 2.25 times than that of plasmin used as standard. Optimal conditions on the growth of B. licheniformis HK-12 associated with the fibrinolytic activity was initial pH 7.0 and 40
Optimization of the Reaction Conditions and the Effect of Surfactants on the Kinetic Resolution of [R,S]-Naoroxen 2,2,2-Trifluoroethyl Thioester by Using Lipse
Song, Yoon-Seok ; Lee, Jung-Ho ; Cho, Sang-Won ; Kang, Seong-Woo ; Kim, Seung-Wook ;
KSBB Journal, volume 23, issue 3, 2008, Pages 257~262
In this study, the reaction conditions for lipase-catalyzed resolution of racemic naproxen 2,2,2-trilfluoroethyl thioester were optimized, and the effect of surfactants was investigated. Among the organic solvents tested, the isooctane showed the highest conversion (92.19%) in a hydrolytic reaction of (S)-naproxen 2,2,2-trifluoroethyl thioester. In addition, the isooctane induced the highest initial reaction rate of (S)-naproxen 2,2,2-trifluoroethyl thioester (
), the highest enantioselectivity (E = 36.12) and the highest specific activity (
) of lipase. Furthermore, reaction conditions such as temperature, concentration of the substrate and enzyme, and agitation speed were optimized using response surface methodology (RSM), and the statistical analysis indicated that the optimal conditions were
, 3.51 mM, 30.11 mg/mL and 180 rpm, respectively. When the optimal reaction conditions were used, the conversion of (S)-naproxen 2,2,2-trifluoroethyl thioester was 96.5%, which is similar to the conversion (94.6%) that was predicted by the model. After optimization of reaction conditions, the initial reaction rate, lipase specific activity and conversion of (S)-naproxen 2,2,2-trifluoroethyl thioester increased by approximately 19.54%, 19.12% and 4.05%, respectively. The effect of surfactants such as Triton X-100 and NP-10 was also studied and NP-10 showed the highest conversion (89.43%), final reaction rate of (S)-naproxen 2,2,2-trifluoroethyl thioester (
) and enantioselectivity (E = 59.24) of lipase.
Surface modification of Poly-(dimethylsiioxane) using polyelectrolYte multilayers and its characterization
Shim, Hyun-Woo ; Lee, Chang-Hee ; Lee, Ji-Hye ; Hwang, Taek-Sung ; Lee, Chang-Soo ;
KSBB Journal, volume 23, issue 3, 2008, Pages 263~270
A poly-(dimethylsiloxane) (PDMS) surface modified by the successive deposition of the polyelectrolytes, poly-(allylamine hydrochloride) (PAH), poly-(diallyldimethylammoniumchloride) (PDAC), poly-(4-ammonium styrenesulfonic acid) (PSS), and poly-(acrylic acid) (PAA), was presented for the application of selective cell immobilization. It is formed via electrostatic attraction between adjacent layers of opposite charge. The modified PDMS surface was examined using static contact angle measurements and fourier transform infrared (FT-IR) spectrophotometer. The wettability of the PDMS surface could be easily controlled and functionalized to be biocompatible through regulation of layer numbers. The modified PDMS surface provides appropriate environment for adhesion to cells, which is essential technology for cell patterning with high yield and viability in the patterning process. This method is reproducible, convenient, and rapid. It could be applied to the fabrication of biological sensing, patterning, microelectronics devices, screening system, and study of cell-surface interaction.
Extraction Condition from Schizandra chinensis Baillon for Beverage Develooment of High Scizandrin Concentration
Choi, Eun-Oh ; Lee, Bong-Soo ; Park, Yong-Su ; Seo, Eun-Ok ; Chung, Bong-Woo ;
KSBB Journal, volume 23, issue 3, 2008, Pages 271~275
Omija (Schizandra chinensis Baillon) has five lignans such as schizandrin, gomisin A, gomisin N, gomisin C and schizandrin C. Its major constituent, schizandrin, has a deterrent effect on nephrotoxicity of cisplatin, blood sugar and an ulcer of the stomach. In this study, we have investigated optimized extraction condition to make high functional beverage by quantifying schizandrin and measuring of browning index. Browning index was used as an index of color deterioration of anthocyanin. Browning index was decreased according to increment in extraction temperature and ethanol concentration. The optimum extraction time, extraction temperature and alcohol concentration levels were 3 hrs,
and 24% alcohol for high schizandrin concentration and desired browing index.
Study on Optimizing, Pretreatment & Simultaneous Saccharification and Fermentation Process for High-efficiency Bioethanol
Choi, Gi-Wook ; Han, Min-Hee ; Kim, Yule ;
KSBB Journal, volume 23, issue 3, 2008, Pages 276~280
In this study, the productivity of bioethanol obtained from various domestic raw materials (barley, brown rice, corn and sweet potato) by simultaneous saccharification and fermentation (SSF) process was estimated. Also, optimal conditions of temperature, time and enzyme concentration in gelatinization and liquefaction process were investigated. As a result, corn showed high ethanol yield of 90.45% and sweet potato had a rapid fermentation time. Productivity of bioethanol increases in accordance with the starch value of raw materials except brown rice. Therefore, it is very important to understand the structure of starch. Further studieson the characteristics of raw materials are necessary to enhance the productivity of bioethanol.