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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 23, Issue 6 - Dec 2008
Volume 23, Issue 5 - Oct 2008
Volume 23, Issue 4 - Aug 2008
Volume 23, Issue 3 - Jun 2008
Volume 23, Issue 2 - Apr 2008
Volume 23, Issue 1 - Feb 2008
Selecting the target year
Microwave-assisted extraction of paclitaxel from plant cell cultures
Hyun, Jung-Eun ; Kim, Jin-Hyun ;
KSBB Journal, volume 23, issue 4, 2008, Pages 281~284
A simple and efficient microwave-assisted extraction procedure was developed and optimized for the extraction of paclitaxel from the plant cell cultures of Taxus chinensis. The biomass, immersed in a methanol-water mixture, was irradiated with microwaves in a closed-vessel system. The microwave-assisted extraction was compared with the existing conventional solvent extraction in terms of yield, extraction time, and solvent consumption. The use of microwave energy allows rapid recovery of paclitaxel from biomass and dramatically reduces extraction time and solvent usage compared to conventional solvent extraction. The paclitaxel was completely extracted from biomass by microwave-assisted extraction for 3 min at
, for 6 min at
The Effects of Environmental Factors on Biodegradability Test for Lubricant Products
Cho, Eun-Hye ; Park, Keun-Hyoung ; Han, Seung-Ock ; Kim, Eui-Yong ; Ryu, Jae-Sang ; Jang, Sun-Bok ; Lee, Un-Gi ; Chae, Hee-Jeong ;
KSBB Journal, volume 23, issue 4, 2008, Pages 285~290
Various methods (OECD 301B, ISO 9439 and ASTM 5864) for biodegradability test of lubricants were reviewed, and a standard procedure was developed. Most lubrication products are released in rivers or sea then is degraded by microbial action in aerobic condition. Most international method are based on
evolution test. Inoculum obtained from a sewage disposal plant and test compound are cultivated in a mineral medium. Organic carbon of the test compound is degraded and oxidized through the enzymatic actions of inoculum, and ultimately mineralized to carbon dioxide. Biodegradability test conditions of lubricant oils were optimized. The highest biodegradability was achieved when the same medium as in ASTM 5864 and inoculum concentration of
cell/L were used. The optimum standard materials were selected as aniline and sodium acetate. Additionally the effects of inoculum type on microbial growth and biodegradability were examined. Finally the standard operating procedure (SOP) for biodegradability test method was proposed.
Evaluation of Osteoinduction Efficacy of Human Amniotic Membrane
Han, Jung-Wook ; Seo, Young-Kwon ; Park, Jung-Keug ; Song, Kye-Yong ;
KSBB Journal, volume 23, issue 4, 2008, Pages 291~296
Amniotic membrane (AM) has been used in various medical application such as biomaterials and it has a biocompatibility and wound healing effects. In this studies, we made AM sponge that was homogenized with AM and then lyophilized. And osteoinduction efficacy of AM sponge was evaluated with collagen sponge by mesenchymal stem cell culture and implantation in nude mouse. As a result of this study, adhesion and proliferation of MSC cells on AM sponge and collagen sponge were not different, but AM sponge was more superior to collagen sponge for induction of collagen secretion and calcium adhesion in matrix in vivo. Besides, AM sponges were more positive stained than collagen sponge about osteocalcin and osteonectin. As a results of this study, there is possibility of doing that AM could increase osteoinduction.
Enhanced extraction and Antioxidant activity analysis of Flavone C-glycosides from Black bamboo using Ultrasonic wave
Choi, Sun-Do ; Lee, Kwang-Jin ;
KSBB Journal, volume 23, issue 4, 2008, Pages 297~302
In this work, the amounts of Flavone C-glycosides homoorientin, orientin extracted from Black bamboo by various ultrasonic waves frequency (35, 72, 170 KHz, 300 Watt
1) time (15, 30, 60 min) and temperature (
) were compared using 50% aqueous ethanol solution. And describesanalysis of the antioxidant potential of Black bamboo using an high-performance liquid chromatography (HPLC) on-line
antioxidant screening method. In conjunction with the analysis of their 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (
) radical scavenging ability. The optimum operating conditions were experimentally determined to analyze the Flavone C-glycosides homoorientin, orientin in the pretreatment ultrasonic extracts. From the results, the effect on extraction yield of variations in frequency, solvent composition and extraction time was investigated. The highest yield of Black bamboo was obtained by ultrasonic waves with a frequency of 72 KHz and an extraction time of 60 min, The extraction frequency 35 KHz (
), time 30 min was selected as an optimal antioxidant activity condition.
Real-Time AT-PCR for Quantitative Detection of Bovine Parainfluenza Virus Type 3 during the Manufacture of Biologics
Lee, Dong-Hyuck ; Kim, Chan-Kyong ; Kim, Tae-Eun ; Kim, In-Seop ;
KSBB Journal, volume 23, issue 4, 2008, Pages 303~310
Bovine blood, cell, tissue, and organ are used as raw materials for manufacturing biologics such as biopharmaceuticals, tissue-engineered products, and cell therapy. Manufacturing processes for the biologics have the risk of viral contamination. Therefore viral validation is essential in ensuring the safety of the products. Bovine parainfluenza virus type 3 (BPIV3) is one of the common bovine pathogens and has widely been known as a contaminant of biologics. In order to establish the validation system for the BPIV3 safety of biologics, a real-time RT-PCR method was developed for quantitative detection of BPIV3 contamination in raw materials, manufacturing processes, and final products. Specific primers for amplification of BPIV3 RNA was selected, and BPIV3 RNA was quantified by use of SYBR Green I. The sensitivity of the assay was calculated to be 2.8
. The real-time RT-PCR method was validated to be reproducible and very specific to BPIV3. The established real-time RT-PCR assay was successfully applied to the validation of Chinese hamster ovary (CHO) cell artificially infected with BPIV3. BPIV3 RNA could be quantified in CHO cell as well as culture supernatant. Also the real-time RT-PCR assay could detect 7.8
of BPIV3 artificially contaminated in bovine collagen. The overall results indicated that this rapid, specific, sensitive, and robust assay can be reliably used for quantitative detection of BPIV3 contamination during the manufacture of biologics.
Melanin Inhibitory Effect and Anti-inflammatory Effects of Dietyota coriacea Extracts Derived from Adjacent Sea of the Jeju Island
Kang, Min-Chul ; Lee, Ju-Yeop ; Ko, Ryeo-Kyeoung ; Kim, Haeng-Bum ; Hong, Seung-Ho ; Kim, Gi-Ok ;
KSBB Journal, volume 23, issue 4, 2008, Pages 311~316
We investigated several biological activities using the ethanol extract and its fractions from Dictyota coriacea to evaluate the usefulness of its extract as a functional biomaterial. The ethanol extract and n-hexane and ethyl acetate fractions showed dependently inhibitory effect on tyrosinase activity and melanin content in B16F10 cells. The ethanol extract and its fractions showed inhibitory effect on Tyrosinase and TRP-1 gene transcription but didn't showed inhibitory effect on TRP-2 gene transcription. Also, the n-hexane and ethyl acetate fractions dose-dependently inhibited the NO production in a RAW 264.7 cells. These results suggest that extract of Dictyota coriacea could be used as functional biomaterial in developing a skin whitening agent having the anti-inflammatory activity.
A Study on the On-line Measurement of Biochemical Oxygen Demand of livestock Wastewater
Kim, Hyoung-Mo ; Kim, Jin-Kyong ; Shin, Kwan-Suk ; Kim, Jun-Hyung ; Jung, Jae-Chil ; Kim, Tai-Jin ;
KSBB Journal, volume 23, issue 4, 2008, Pages 317~322
The Biochemical Oxygen Demand(BOD) is one of important parameters for the most widely used method of organic pollution in wastewater and wastewater treatment effluent. As the conventional BOD test needs 5-day long incubation period, it is thus incompatible with real time control of wastewater treatment plant. To resolve this problem, in the present study an on-line Dissolved Oxygen(DO) monitoring system was used to observe the transient change of dissolved oxygen concentration in livestock wastewater. The system was composed of BOD sensor, amplifier and computer. It was observed that the concentration of the microorganism in the livestock wastewater was relatively constant during the growth period of initial one hour, which allowed the assumption of the constant Oxygen Uptake Rate(OUR) within one hour of measurement. It was thus concluded that the present scheme provided a protocol for automatic measurement of BOD in livestock wastewater, which can be applicable to optimal control of livestock wastewater treatment plant.
A Study on the Removal of Organics and Nutrients in the Process Using Attached Biomass and Aquatic Floating Plants
Seon, Yong-Ho ;
KSBB Journal, volume 23, issue 4, 2008, Pages 323~328
This study was accomplished using Anaerobic/Anoxic/Oxic biofilm reactors with fixed media and post-treatment reactor for natural purification with aquatic floating plants. The objectives of this study was to investigate the characteristics of organics, nitrogen and phosphorus removal from sewage with the HRT. The average removal efficiency of SS and
increases as increasing the hydraulic retention time (HRT) until 12 hr of the HRT, and it was constant over 12 hr of the HRT. The removal efficiency of them was about 93% and 89% respectively over the 12 hr of HRT. The average
increases as increasing the HRT and the removal efficiency of them was 84.91 % and 76.03% respectively at the 26 hr of HRT. The removal efficiency of T-N and T-P increases as increasing the HRT until 61 hr of the HRT, and it was constant over 61 hr of the HRT. At the HRT of 61 hr, it was 70.20%, 77.86% respectively. It was found that the optimum HRT was 61 hr in case of the nutrients. Before and after experiment, the nitrogen content was similar in leaves of the water hyacinths but the nitrogen content in roots after experiment was 5.5% more than its content before experiment. It was known that the nitrogen was absorbed by the water hyacinths.
Antioxidant activity of partially purified extracts isolated from Acanthopanax sessiliflorum Seeman
Im, Kyung-Ran ; Kim, Mi-Jin ; Jung, Teak-Kyu ; Yoon, Kyung-Sup ;
KSBB Journal, volume 23, issue 4, 2008, Pages 329~334
The antioxidant activity and the qualitative analysis of Acanthopanax sessiliflorum Seeman were studied by partially purified extract using various methods: extraction by using ethanol solutions and temperatures, and absorption to Diaion HP20 column chromatography using 10%, 20%, 40%, 60% ethanol solutions. Major constituents, chlorogenic acid, caffeic acid, eleutheroside E, was determinated by HPLC method in Acanthopanax sessiliflorum S. 10% and 20% ethanol solutions contain chlorogenic acid (3.020
1.150%, respectively). 40% ethanol solution contains caffeic acid and eleutheroside E (12.270
0.140%, respectively). Diaion HP20 fractions (10%, 20%, 40%, 60% ethanol solutions) showed the scavenging activity of radicals and reactive oxygen species with the
against 1,1-diphenyl-2-picrylhydrazly radical and
against superoxide radicals in the xanthine/xanthine oxidase system, respectively. Especially, 20% and 40% ethanol fractions showed more antioxidant activity than dl-
-tocopherol. These results suggest that Acanthopanax sessiliflorum S. extract and Diaion HP20 fractions may be useful as a potential source of nutraceutical and cosmetic products.
Effects of Properties of Raw Materials on Biodiesel Production
Jeong, Gwi-Taek ; Park, Seok-Hwan ; Park, Jae-Hee ; Park, Don-Hee ;
KSBB Journal, volume 23, issue 4, 2008, Pages 335~339
Biodiesel is an alternative and renewable energy source, which is hoped to reduce global dependence on petroleum and environmental problem. Biodiesel produced from a variety of oil sources such as vegetable oil, animal fat and waste oils, and has properties similar to those associated with petro-diesel, including cetane number, volumetric heating value, flash point, viscosity and so on. In this study, we investigate the effect of quality of raw materials on alkali-catalyzed transesterification for producing of biodiesel. The increase of content of free fatty acid and water in oil were caused the sharp decrease of conversion yield. Also, the low purity of methanol in reactant was inhibited the reaction rate. In the case of addition of sodium sulfate as absorbent to prepare catalyst solution, the content of fatty acid methyl ester in product was increased more about 1.6% than that of control. However, the addition of zeolite, sodium chloride and sodium sulfate as absorbent in reactant to remove water generated from reaction did not show any enhancement in the reaction yield. This result may provide useful information with regard to the choice and preparation of raw materials for more economic and efficient biodiesel production.
In the presence of organic solvent stability of CiP [coprinus cinereus peroxidase]
Kim, Han-Sang ; Cho, Dae-Haeng ; Kim, Yong-Hwan ;
KSBB Journal, volume 23, issue 4, 2008, Pages 340~344
Coprinus cinereus peroxidase (CiP) was often used as a catalyst for oxidative polymerization of a variety of phenol derivatives to produce a new class of polyphenols. Economical point of view, to know the mechanism of enzyme deactivation is significantly important because cost of enzyme is critically high. Hydrogen peroxide being used as oxidizing agent induced deactivation of peroxidase by destruction of heme structure. In the presence of hydrogen peroxide the stability of peroxidase was unexpectedly improved by adding organic solvent. Especially 2-propanol significantly improved enzyme stability among tested solvents. Radical scavenging by organic solvents may play a major role in protecting peroxidase from the oxidation of oxidizing radicals.
Optimization of Solid-State Fermentation Condition Using Distiller's Dried Grain
Choi, Gi-Wook ; Moon, Se-Kwon ; Kim, Yule ; Jang, Byung-Wook ; Kim, Young-Ran ; Chung, Bong-Woo ;
KSBB Journal, volume 23, issue 4, 2008, Pages 345~349
To enhance the value as a feedstuff of distiller's dried grain (DDG) and develop fermented feedstuff, we investigated the effects of the culture conditions affecting glucoamylase activity, such as pH in submerged culture and moisture content in solid-state culture. Also, we investigated the optimal mixing ratio of DDG and wheat bran for the production of fermented feedstuff containing high content of amino acids. In culture conditions for high fermented activity, pH and moisture were optimum at pH 4 and 60%, respectively. In the case of mixing ratio, the glucoamylase activity was decreased with increase of DDG content. On the other hand, the content of crude protein was increased slowly. For the development of fermented feedstuff, the optimal mixing ratio of DDG and wheat bran was 1 to 4. Finally, we could produce approximately 1 ton (dry matter) of trial product in incubator of pilot-scale. The glucoamylase activity and the crude protein content were 1,024 U/g and 33.6%, respectively.
Development of Magnetically Separable Immobilized Trypsin
Ryu, Ji-Soon ; Lee, Jung-Heon ;
KSBB Journal, volume 23, issue 4, 2008, Pages 350~354
Magnetically separable immobilized trypsin was developed and their biocatalytic activity was evaluated for the different immobilization media. The activity, recyclability, pH effect, and stability of immobilized enzymes were evaluated for the different supporting media. The biocatalytic activity of immobilized trypsin was highest with magnetically separable polyaniline (PAMP), and Vm and Km of PAMP were 0.169 mM/min and 0.263 mM respectively. With increasedpH, the biocatalytic activity increased for all supporting materials used. Immobilized enzymes were recycled and recycle activities were over 90% of their original activity after ten times reuse. The operational stabilities of enzymes were greatly improved with enzyme immobilization.