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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 23, Issue 6 - Dec 2008
Volume 23, Issue 5 - Oct 2008
Volume 23, Issue 4 - Aug 2008
Volume 23, Issue 3 - Jun 2008
Volume 23, Issue 2 - Apr 2008
Volume 23, Issue 1 - Feb 2008
Selecting the target year
Incorporation of Unnatural Amino Acids into Proteins
Yun, Hyung-Don ;
KSBB Journal, volume 23, issue 5, 2008, Pages 355~361
Proteins carrying unnatural amino acids with novel side chains add a new dimension to studies of protein structure and function. This article provides an overview of the various strategies that have been developed to date for the synthesis of such proteins.
Application of Biological industry using High Hydrostatic Pressure (HHP) system
Lee, Kwang-Jin ; Choi, Sun-Do ;
KSBB Journal, volume 23, issue 5, 2008, Pages 362~368
High Hydrostatic Pressure assisted (HHP) process enhancement for food and allied industries are reported in this paper review. Recently, considerable research has been devoted to the improvement of mild thermal processing techniques and to the development of alternative mild processing technologies. HHP assisted can enhance existing extraction, processes and enable new commercial extraction opportunities and processes. New HHP processing approaches have been proposed, including, the potential for modification of plant cell material to provide improved bioavailability of micro nutrients while retaining the natural-like quality, simultaneous extraction. Therefore, High Hydrostatic Pressure assisted (HHP) technologies could have a strong presence in the future of the biotechnology industry.
Asymmetric resolution of racemic styrene oxide using recombinant Escherichia coli harboring epoxide hydrolase of Rhodotorula glutinis
Park, Kyu-Deok ; Choi, Sung-Hee ; Kim, Hee-Sook ; Lee, Eun-Yeol ;
KSBB Journal, volume 23, issue 5, 2008, Pages 369~374
The effects of reaction temperature and the addition of various detergents on the enantioselective hyrolysis activity of the recombinant Escherichia coli containing the epoxide hydrolase (EH) gene of Rhodotorula glutinis were investigated for the production of enantiopure styrene oxide. The recombinant E. coli harboring the EH gene from R. glutinis exhibited the enantiopreference toward (R)-styrene oxide with the maximum hydrolytic activity of
of dry cell weight (dcw). The addition of 0.5% (w/v) Tween 20 at
increased the initial hydrolysis rate and enantioselectivity by 1.45-fold and 2.0-fold, respectively. Enantiopure (S)-styrene oxide was prepared with 99% ee enantiopurity and 46.0% yield (theoretical yield=50%) from 20 mM racemic styrene oxide.
Effect of Gill, Drying and Temperature and Chitosan-Ascorbate Treatment on Growth of Contaminated Microorganism in Flounder during Storage
Kim, Young-Sook ;
KSBB Journal, volume 23, issue 5, 2008, Pages 375~380
We made an experiment of keeping extension of raw and semi-dried flounder (Pleuronectes herzensteini). Effect of with(WG) or without gill (OG), drying degree (20% drying：20D, 40% drying：40D) and storage temperature (5 and
) and 0.1% chitosan-ascorbate (CA) treatment of vacuum packaging flounder on growth of contaminated microorganism during storage for 10 days were investigated. Total aerobacter (TA) in the OG-treated raw flounder was
log cycle lower than that of WG-treated flounder and also, number of coliform (CF) and E. coli (CO) in OG were lower compared with WG. Number of TA,. Especially, the TA was
log cycle lower compared with raw flounder. The TA of the raw flounder stored at
log cycle lower. The growth of total aerobacter, coliform (and E. coli separated from raw flounder in tryptic soy broth were completely inhibited by 0.1% CA. But the growth of TA in the raw and 20% dried flounder was 1.5 log cycle inhibited by 0.1% CA, and the growth of CF and CO were also slightly inhibited. We did value raw sample that treated CA 0D and 20D, and did vaccum packaging at the
for 10 days, Sensory quality such as appearance, freshness, and texture and overall acceptability after cooking of the 0.1% CA-treated raw and 20% dried flounder were evaluated from good to very good, while CA non-treated products were evaluated to moderate.
Metronidazole Reduced Ammonia Toxicity in Human Hep G2 cell and Rat Hepatocytes
Kim, Bo-Ae ; Kim, Hyun-Jung ; Kim, You-Young ;
KSBB Journal, volume 23, issue 5, 2008, Pages 381~386
Lipophilic ammonia is toxic gas and can easily diffuse across cell membranes. Excess ammonia is implicated in the pathogenesis of several metabolic disorders including hepatic encephalopathy and may result in the death. The purpose of this study was to clarify the inhibition effect of metronidazole on liver cell damage due to ammonia in human Hep G2 cell and rat hepatocytes. The effects of metronidazole were studied in ammonium chloride treated human Hep G2 cell (75 mM) and rat hepatocyte (100 mM) following
metronidazole treatment. In MTZ+AC group, cell viabilities increased prominently and LDH activities decreased over 25% than AC group. Furthermore, ammonia level according to ammonium chloride treatment reduced over 30% and lipid peroxidation as an index of cell membrane damage decreased more than twice. By comparison with control, catalase activity showed more than 30% reduction in AC group while less than 10% reduction in MTZ+AC group, respectively. In addition, MTZ+AC group showed the similar cell structure as control in cell morphology study by using light microscope, and represented fluorescent intensity decrement compared with AC group in fluorescent microscopic study with avidin-TRITC fluorescent dye. And cleaved PARP expression due to ammonia reduced twofold or more in MTZ+AC group. As the results suggest, metronidazole may protect the liver cell by inhibiting cell damages due to ammonia and be used for an effective antagonist of ammonia in hyperammonemia.
Nutritional Component Analysis of Green Tea Tree's Root and Seed
Cha, Wol-Suk ; Cho, Mi-Ja ; Ding, Ji-Lu ; Shin, Hyun-Jae ;
KSBB Journal, volume 23, issue 5, 2008, Pages 387~391
Green tea is popular plants in Asian countries and has become more widespread in western countries due to its taste characteristics and health benefits. Apart from green tea leafs, however, the use of root and seed of green tea tree has not intensively been investigated yet. In this study, the contents of mineral, vitamin, total amino acid, free amino acid, and total polyphenol (catechin, caffeic acid, and chlorogenic acid) of the root and seed of green tea tree were analyzed for the development of healthy foods. For minerals, potassium contents were 1,052 and 1,480 mg/100g-dry weight of root and seed, respectively. The order of mineral contents were as follows: K > P > Ca > Mg > Na > Fe > Mn > Zn > Cu in root and K > P > Na > Ca > Mg > Mn > Fe > Cu > Zn in seed. For vitamins, vitamin C contents were 5.72 and 6.05 mg/100g-dry weight of root and seed, respectively. The presence of more various kinds of vitamins were observed in seed than in root. For total amino acids, the contents were 1,651 and 4,335 mg/100g-dry weight of root and seed, respectively. The total amino acid contents of seed and root were higher than those in commercial green tea products. Especially the phenylalanine contents were 16 and 139 mg/100g-dry weight of root and seed, respectively whereas phenylalanine was not found in commercial green tea products. Concerning free amino acids, the bitter tasting amino acids such as arginine, valine and tryptophan were more abundant in root and the sweet tasting ones such as glutamic acid, alanine, aspartic acid, and serine were more abundant in seed. The total polyphenol contents were 237 and 81 mg/100g-dry weight of root and seed, respectively. The polyphenol contents in root were three times higher than that in seed so root may be a better source for antioxidant ingredients than seed. Among many polyphenols, catechin, caffeic acid, and chlorogenic acid were the top three major components.
Comparison of Mycophenolic Acid Production by P. brevicompactum Mutants Induced through UV and NTG Treatments
Um, Byung-Hwan ; Choi, Ju-Young ; Ha, Byung-Jhip ; Kim, Young-Soo ; Oh, Kyeong-Keun ;
KSBB Journal, volume 23, issue 5, 2008, Pages 392~397
Recently, importance of immunosuppression is increasing as internal organ transplant becomes more prevalent with development of medical technology. Mycophenolic acid (MPA) is a selective inhibitor of guanine synthesis and it therefore has antibacterial, antiviral, antitumor and selective immunosuppressive activities. The objective of this study was to maximize MPA productivity through utilizing the MPA generating strain of Penicillium brevicompactum ATCC 16024, by inducing UV mutation and NTG mutation. The highest MPA obtained was 1.146 g/L, 2.051 g/L, and 1.390 g/L from P. brevicompactum UB-3, UB-9, UC-4 respectively mutants derived from UV treatment. P. brevicompactum NC-3 and NA-9 induced from NTG treatment yielded. 575 g/L, 2.238 g/L of MPA production respectively. Mutants capable of the highest observed production of MPA were P. brevicompactum UB-9 and P. brevicompactum NC-3 obtained using the UV and NTG treatments respectively.
Extracellular Products from Cyanobacteria
Kwon, Jong-Hee ; Kim, Gi-Eun ;
KSBB Journal, volume 23, issue 5, 2008, Pages 398~402
Cyanobacteria havebeen identified as one of the most promising group producing novel biochemically active natural products. Cyanobacteria are a very old group of prokaryotic organisms that produce very diverse secondary metabolites, especially non-ribosomal peptide and polyketide structures. Though many useful natural products have been identified in cyanobacterial biomass, cyanobacteria produce also extracellular proteins related with NRPS/PKS. Detection of unknown secondary metabolites in medium was carried in the present study by a screening of 98 cyanobacterial strains. A degenerated PCR technique as molecular approaches was used for general screening of NRPS/PKS gene in cyanobacteria. A putative PKS gene was detected by DKF/DKR primer in 38 strains (38.8%) and PCR amplicons resulted from a presence of NRPS gene were showed by MTF2/MTR2 primer in 30 strains (30.6%) and by A3/A7 primer in 26 strains (26.5%). HPLC analysis for a detection of natural products was performed in extracts from medium in which cyanobacteria containing putative PKS or NRPS were cultivated. CBT57, CBT62, CBT590 and CBT632 strains were screened for a production of extracellular natural products. 5 pure substances were detected from medium of these cyanobacteria.
Construction of Candida antarctica Lipase B Expression System in E. coli Coexpressing Chaperones
Jung, Sang-Min ; Lim, Ae-Kyung ; Park, Kyung-Moon ;
KSBB Journal, volume 23, issue 5, 2008, Pages 403~407
Recently, Candida antarctica lipase B (CalB) draws attention from industries for various applications for food, detergent, fine chemical, and biodiesel, because of its characteristics as an efficient biocatalyst. Since many industrial processes carry out in organic solvent and at high temperature, CalB, which is stable under harsh condition, is in demand from many industries. In order to reform CalB promptly, the expression system which has advantages of ease to use and low cost for gene libraries screening was developed using E. coli. The E. coli strains, Rosettagami with competence for enhanced disulfide bond formation, Novablue, and
, were exploited in this study. To obtain the soluble CalB, the pCold I vector expressing the cloned gene at
and the chaperone plasmids containing groES/groEL, groES/groEL/tig, tig, dnaK/dnaJ/grpE, and dnaK/dnaJ/grpE/groES/groEL were used for coexpression of CalB and chaperones. The colonies expressing functional lipase were selected by employing the halo plate containing 1% tributyrin, and the CalB expression was confirmed by SDS-PAGE. E. coli Rosettagami and
harbouring groES/groEL chaperones were able to express soluble CalB effectively. From a facilitative point of view, E. coli
is more suitable for further mutation study.
Preparation and Characterization of Rosiglitazone-loaded PLGA Nanoparticles
Shin, Ko-Eun ; Huh, Kang-Moo ; Lee, Yong-Kyu ;
KSBB Journal, volume 23, issue 5, 2008, Pages 408~412
The rosiglitazone loaded poly (lactide-co-glycolide) (PLGA) nanoparticles (NPs) were prepared by the emulsion-evaporation method and optimized for particle size and entrapment efficiency. The optimized particles were 140-180 nm in size with narrow size distribution and 80% entrapment efficiency at 1% w/w initial drug loading when prepared with 1-3% w/v of PVA as a surfactant. These particulate carriers exhibited controlled in vitro release of rosiglitazone for 36 hrs at a nearly constant rate after 4 hrs release. In conclusion, these results indicate that PLGA NPs have greater potential for oral delivery of rosiglitazone.
Biological production of 1,3-propanediol using crude glycerol derived from biodiesel process
Jun, Sun-Ae ; Kang, Cheol-Hee ; Kong, Sean-W. ; Sang, Byoung-In ; Um, Young-Soon ;
KSBB Journal, volume 23, issue 5, 2008, Pages 413~418
The production of 1,3.propanediol (1,3-PD) was investigated with Klebsiella pneumoniae DSM2026 and K. pneumoniae DSM4799 using crude glycerol obtained from biodiesel industry. Crude glycerol was used without prior purification to investigate effects of impurities in crude glycerol on 1,3-PD production. In the batch cultures, 1,3-PD production with crude glycerol was
times higher than that with pure glycerol, indicating that crude glycerol is even a better substrate than pure glycerol for 1,3-PD fermentation. When glucose was added, 1,3-PD production and yield decreased in spite of enhanced cell growth. Furthermore, the addition of glucose was found to increase 2,3-butanediol, a by-product, significantly because of the change in metabolism in the presence of glucose. In semi-batch cultures without glucose addition, 26 g/L 1,3-PD was produced with crude glycerol, which was
times higher than that with pure glycerol. Based on our results, it was clearly shown that crude glycerol is an effective substrate for biological 1,3-PD production, making it more feasible to produce 1,3-PD at a lower price.
Inhibitory Activity on the Diabetes Related Enzymes of Tetragonia tetragonioides
Choi, Hye-Jung ; Kang, Jum-Soon ; Choi, Young-Whan ; Jeong, Yong-Kee ; Joo, Woo-Hong ;
KSBB Journal, volume 23, issue 5, 2008, Pages 419~424
In this study, we examined the anti-diabetic activity in vitro by the crude extracts of Tetragonia tetragonioides which has been known to superior plants for the traditional prevention and treatment of stomach-related diseases.
-glucosidase, the principal enzymes involved in the metabolism of carbohydrates, and aldose reductase, the key enzyme of the polyol pathway, have been shown to play the important roles in the complications associated with diabetes. A hexane (HX) fraction of T. tetragonioides were shown to inhibit more than 50% of salivary and pancreatin
-amylase activity at concentration of 2.882 mg/mL and 2.043 mg/mL, respectively. In addition, the HX and ethylacetate (EA) fraction showed the highest inhibitory activity on yeast
-glucosidase at values of
of 0.723 mg/mL and 1.356 mg/mL respectively. The HX, dichloromethane (DCM) and EA fraction showed more higher inhibitory activity on yeast
-glucosidase than commercial agent such as 1-deoxynorjirimycin and acarbose. Also, the aldose reductase from human muscle cell had been inhibited strongly by the DCM fraction and HX fraction at 51.95% and 47.22% at a concentration of 1 mg/mL, respectively. Our study, for the first time, revealed the anti-diabetic potential of T. tetragonioides and this study could be used to develop medicinal preparations or nutraceutical and functional foods for diabetes and related symptoms.
The MALDI-TOF MS determination of yeast proteins producing
Cho, Hyun-Nam ; Fan, Lu-An ; Yoo, Dong-Chan ; Yang, Seun-Ah ; Lee, In-Seon ; Kim, Jae-Hyung ; Baek, Hyo-Hyun ; Jhee, Kwang-Hwan ;
KSBB Journal, volume 23, issue 5, 2008, Pages 425~430
Hydrogen sulfide (
) is a by-product of metabolism of amino acids including sulfur and alcoholic fermentation, it is generally thought of in terms of a poisonous gas. Though
can have a negative impact on the perceived quality of fermented drinks due to an undesirable aroma, it plays prominent roles as a neuromodulator in the mammalian brain as well as a smooth muscle relaxant. Nowadays studies on the proteins which produce
are carried out in various fields such as structure, function, and metabolism. Here we propose to develop a simple and rapid
forming assay method, which will lead to speed up preparing the
forming proteins for identification by MALDI-TOF MS analysis. We detected three kinds of proteins which produce
in the crude extract of Saccharomyces cerevisiae. Those proteins were cystathionie
-synthase, O-acetylserine sulfhydrylase, and cystathionine
The Effect of Celosia cristata L. ethanol Extract on Anti-oxidant & Anti-aging Activity
Pyo, Young-Hee ; Yoon, Mi-Yun ; Son, Ju-Hyun ; Choe, Tae-Boo ;
KSBB Journal, volume 23, issue 5, 2008, Pages 431~438
For the experiment, to develop new materials for cosmetics, the Celosia cristata L. plant ethanol extract were used for physiological effect and cosmetics application research. The Celosia cristata L. is a Korean traditional variety grown. To investigate the effect of Ethanol extract of Celosia cristata L. on skin care, we measured anti-oxidant activity and anti-aging activity. Celosia cristata L. ethanol extract itself had anti-oxidant activity in a dose-dependent manner in 1-diphenyl-2-picryl-hydrazyl(DPPH) radical scavenging. Ethanol extract had anti-oxidant activity in a dose-dependent manner. Silica dose-dependently increased the intracellular ROS generation in RAW 264.7 cells. Celosia cristata L. ethanol extract inhibited silica-induced intracellular superoxide anion generation and
generation and hydro-peroxide generation in RAW 264.7 cells. For anti-aging effects, the hyaluronidase inhibition effects, were relatively strong and they also showed elastase activity inhibition effects, which suggesting the Celosia cristata L. ethanol extract might be used as hydration and anti-wrinkle agents. From the above results, it is referred that Celosia cristata L. ethanol extract appears to have potent anti-oxidant activity and anti-aging activity.
Hair Care effects of hair cosmetics including Low molecular weight silk peptide component and micro structure analysis
Hyun, Ji-Won ; Lee, Kwang-Gill ; Yeo, Joo-Hong ; Choe, Tae-Boo ;
KSBB Journal, volume 23, issue 5, 2008, Pages 439~444
In this study, hair care effects of the hair cosmetics including low molecular weight silk peptide, hydrolysate which is produced from cocoon were investigated. After producing the hair cosmetics including silk peptide which has 300-500 molecular weight, we measured its hair care effects through the various tests; change of the hair weight, hair thickness, absorbance rate and hair moisture value and micro structure analysis. As a result, S.P.T. (Silk peptide Treatment) was effectively penetrated into the hair which has been damaged by chemical treatments, increased the hair weight, thickness and hair moisture value and also recovered the cuticle of the hair. Thus, the treatment of hair with silk peptide hydrolysate would be effective to recover the damaged hair into the normal conditions.
Functional expression of CalB in E.coli
Kim, Hyun-Sook ; Kim, Yong-Hwan ;
KSBB Journal, volume 23, issue 5, 2008, Pages 445~448
Candida antarctica lipase B (CalB) is an efficient biocatalyst for many organic synthesis reactions. To make full use of CalB, we need effective expression system. Previously recombinant CalB was successfully expressed in the methylotropic yeast Pichia pastoris. In addition, we succeed in the functional expression of CalB in the Escherichia coli cytoplasm. This CalB expression system in E.coli has many considerable advantages in comparison with other expression systems and enables high-throughput screening of gene libraries as those derived from directed evolution experiments. To optimize E.coli system, we investigate comparing between OrigamiB (DE3) and BL21 (DE3) and observing effect of IPTG amount.
The ceramide contents of Saccharomyces cerevisiae in batch culture
Kim, Se-Kyung ; Noh, Yong-Ho ; Yun, Hyun-Shik ;
KSBB Journal, volume 23, issue 5, 2008, Pages 449~451
Ceramide has become a widely used ingredient in cosmetic and pharmaceutical industries, however, only a few yeast strains were investigated for the synthesis of ceramide and the concentration was very low. Ceramide is not only a core intermediate of sphingolipids but also an important modulator of many cellular events including apoptosis, cell cycle arrest, senescence, differentiation, and stress responses. In this study S.cerevisiae was grown in a batch culture and the cellular content of ceramide was measured at different growth phases. The ceramide content was highest at stationary phase and 2.01 mg ceramide/g cell was obtained.
Preparation of Biodegradable Polymer Microparticles Containing 5-FU Using Supercritical Carbon Dioxide
Jung, Ju-Hee ; Jung, In-Il ; Joo, Hyun-Jae ; Shin, Jae-Ran ; Lim, Gio-Bin ; Ryu, Jong-Hoon ;
KSBB Journal, volume 23, issue 5, 2008, Pages 452~459
To obtain maximal efficacy with minimal systemic side-effects, many studies have been carried out to achieve the controlled release of 5-fluorouracil (5-FU). In this study, biodegradable poly(L-lactide) (L-PLA) microparticles containing 5-FU were prepared by a process, called aerosol solvent extraction system (ASES), utilizing supercritical carbon dioxide. The effects of various organic solvents, drug/polymer feeding ratio, polymer molecular weight, and blending with the same polymers with different molecular weights on the formation of 5-FU loaded microparticles were investigated under a predetermined operating condition from our previous study. The drug recovery, entrapment efficiency, and in vitro drug release kinetics were determined by HPLC assays. The drug recovery obtained from the ASES process was found to be very high, whereas the drug entrapment efficiency was considerably low in all the experiments due to the poor affinity between L-PLA and 5-FU. These results indicated that the precipitation rate of L-PLA might be quite different from that of 5-FU so that there was little chance to form 5-FU loaded L-PLA microparticles.
Utilization of pollen grains for the expression of epidermal growth factor
Choi, Byung-Jin ; Park, Hee-Sung ;
KSBB Journal, volume 23, issue 5, 2008, Pages 460~462
Pollens grains collected from fully dehisced lily (Lilium longiflorum) anthers were given wounds by means of shaking in the presence of aluminum oxide particles. They were transformed by infiltration with Agrobacterium cells harboring a synthetic DNA encoding signal peptide-fused epidermal growth factor (EGF). After incubation for 24 hr in vitro, the pollen culture showed that EGF mRNAs and proteins were successfully expressed in the analysis of cDNA blot hybridization and immuno-blotting.