Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal Basic Information
Journal DOI :
Korean Society for Biotechnology and Bioengineering
Editor in Chief :
Volume & Issues
Volume 23, Issue 6 - Dec 2008
Volume 23, Issue 5 - Oct 2008
Volume 23, Issue 4 - Aug 2008
Volume 23, Issue 3 - Jun 2008
Volume 23, Issue 2 - Apr 2008
Volume 23, Issue 1 - Feb 2008
Selecting the target year
Production Medium Optimization for Monascus Biomass Containing High Content of Monacolin-K by Using Soybean Flour Substrates
Lee, Sun-Kyu ; Chun, Gie-Taek ; Jeong, Yong-Seob ;
KSBB Journal, volume 23, issue 6, 2008, Pages 463~469
During the last decade, monacolin-K biosynthesized by fermentation of red yeast rice (Monascus strains) was proved to have an efficient cholesterol lowering capability, leading to rapid increase in the market demand for the functional red yeast rice. In this study, the production medium composition and components were optimized on a shake flask scale for monacolin-K production by Monascus pilosus (KCCM 60160). The effect of three different soybean flours on the monacolin-K production were studied in order to replace the nitrogen sources of basic production medium (yeast extract, malt extract and beef extract). Among the several experiments, the production medium with dietary soybean flour to replace a half of yeast extract was very good for monacolin-K production. Plackett-Burman experimental design was used to determine the key factors which are critical to produce the biological products in the fermentation. According to the result of Plackett-Burman experimental design, a second order response surface design was applied using yeast extract, beef extract and
as factors. Applying this model, the optimum concentration of the three variables was obtained. The maximum monacolin-K production (369.6 mg/L) predicted by model agrees well with the experimental value (418 mg/L) obtained from the experimental verification at the optimal medium. The yield of monacolin-K was increased by 67% as compared to that obtained with basic production medium in shake flasks.
Removal of VOC compounds in the vent of a pharmaceutical plant using a pilot-scale biofilter
Ryu, Hee-Wook ; Lee, Tae-Ho ; Park, Chang-Ho ;
KSBB Journal, volume 23, issue 6, 2008, Pages 470~473
A pilot-plant biofilter (
) packed with polyurethane foam (
) was installed in an pharmaceutical plant emitting gas streams containing n-hexane and alcohols. The biofilter was successfully operated for 74 days under highly fluctuating incoming concentrations at a residence time of 12.8-24.8 sec. Alcohols and n-hexane were removed by more than 90% from 5 and 20 days after start up, respectively. Malodor was also removed more than 95% from 20 days after start up.
Disaccharide Synthesis using E. coli UDP-glucose regeneration system
Oh, Jeong-Seok ;
KSBB Journal, volume 23, issue 6, 2008, Pages 474~478
UDP-glucose regeneration system using metabolic engineeringis unique and efficient strategy for oligosaccharide synthesis. To exploit the efficient UDP-glucose regeneration system, we introduced four enzymes, which would be important in partitioning the flux of UDP regenerationsuch as UDP-glucose pyrophosphorylase, UDP-Kinase gene, UDP-galactose 4-epimerase, and
-1, 4-galactasyltrasnsferase, into E. coli AD202. To determine the optimal expression level for UDP-regeneration, LacNAc concentration was compared depending on IPTG concentration. 0.5 mM IPTG induction showed the higher oligosaccharides synthesis. Using metabolic engineering under optimal IPTG induction, LacNAc synthesis of AD202/pQNGLU increased until 16 h and showed the 1.34 mM. This concentration is 10 times higher than that of control strain at same reaction time. Lactose of AD202/pQNGLU showed the maximum synthesis of 0.39 mM at 16 h and showed the 2.6 times higher than that of control strain.
Biodegradation of JP-8 in soil column by Rhodococcus fascians isolated from petroleum contaminated soil
Park, Bong-Je ; Noh, Yong-Ho ; Yun, Hyun-Shik ;
KSBB Journal, volume 23, issue 6, 2008, Pages 479~483
The environmental contamination by organic pollutants is a widespread problem. The most widely distributed pollution can be attributed to oil contamination. Bioremediation, the use of microorganism or microbial processes to degrade environmental contaminant, is one of the new technologies. The objective of the present study is to study the degradation of JP-8 in soil by microorganism. The degradation of JP-8 was analysed by TPH using gas chromatography. Rhodococcus fascians isolated from the petroleum contaminated site was applied for the degradation of JP-8 in the soil column system. Air flow rate of 30 ml/min was sufficient to degrade JP-8 in the soil column as much as 70% of JP-8 in the soil column. The addition of nitrogen source resulted in the increase in JP-8 degradability to 75% of JP-8 and the C:N ratio for JP-8 degradation was 100:10.
Rapid Screening Method of Peroxidase by Colorimetric Assay and Screening of 2, 4-DCP Degradable Strains
Ryu, Kang ; Lee, Eun-Kyu ;
KSBB Journal, volume 23, issue 6, 2008, Pages 484~488
Chlorinated phenols are widely used by the chemical industry as intermediate products in synthesis and previously were frequently applied to various industry fields. Peroxidases catalyze the peroxide-dependent oxidation of a range of inorganic and organic compounds. Peroxidase was shown to mineralize a variety of recalcitrant aromatic compounds and to oxidize a number of polycyclic aromatic and phenolic compounds. Among monomeric phenolic and nonphenolic compounds, peroxidase is known to oxidize its compounds. In this study, a colorimetric assay was developed to quantitatively evaluate the peroxidase activity for rapid screening. Color products of different intensity were developed proportionally to the peroxidase activity on agar plate and 96-well plate. This method correlates well with the RP-HPLC result. Using this screening method, 12 colonies of strain was screened which survived at high concentration of 2,4-DCP (1000 ppm) and with peroxidase activity for the
round screening step on agar plate. These strains were utilized 2,4-DCP as a sole carbon source and produced peroxidase. After the screening test, four of the bacteria have significant better effect of COD removal on dye waste-water. COD removal of these was from 44% to 61%, respectively.
Changes of the Protein Contents of Seafood Cooking Drips by Gamma Irradiation
Choi, Jong-Il ; Kim, Hyun-Joo ; Sung, Nak-Yun ; Byun, Eui-Baek ; Kim, Jae-Hun ; Chun, Byung-Soo ; Ahn, Dong-Hyun ; Cho, Kook-Yeon ; Byun, Myung-Woo ; Lee, Ju-Woon ;
KSBB Journal, volume 23, issue 6, 2008, Pages 489~493
Although the seafood cooking drips were the byproducts from the fishery industry and being wasted, it had many nutrients including proteins. In this study, the effect of a gamma irradiation on the cooking drips from Hizikia fusiformis, Enteroctopus dofleni and Thunnus thynnus were investigated. The cooking drips were extracted with 70% ethanol solution, and the extracts were analysed for the protein concentration by three different methods of Lowry, BCA and Kjeldahl. The extracts were irradiated with different doses and the protein contents were compared with respect to the absorbed doses. Total content of the proteins was increased with increasing irradiation dose. The change of protein pattern in the irradiated cooking drips was also confirmed by SDS-PAGE analysis. These results shown that the proteins in cooking drips could be unfolded or aggregated by the irradiation. Therefore, gamma irradiation could be considered as an effective method for extracting useful proteins.
Bioethanol production from wood biomass hydrolysate with supercritical water treatment
Seo, Hyeon-Beom ; Han, Jae-Gun ; Choi, Won-Seok ; Lee, Oh-Kyu ; Lee, Soo-Min ; Choi, Seok-Hwan ; Lee, Hyeon-Yong ; Jung, Kyung-Hwan ;
KSBB Journal, volume 23, issue 6, 2008, Pages 494~498
We investigated the bioethanol production using wood biomass hydrolysate which obtained from the supercritical water (SCW) treatment. SCW-treated hydrolysate was used C-source of culture medium in shaking flask culture for bioethanol production. When the concentrated SCW-treated hydrolysate (SCW3) was used, yeast cell growth was slower compared with those in other SCW-treated hydrolysate (SCW1, SCW2). In addition, the bioethanol productions were 0.51 to 0.56 (%,w/v) when SCW1, SCW2, and SCW3 were used. Therefore, we removed the toxic phenolic compound in SCW-treated hydrolysate by pretreatments of activated charcoal and calcium hydroxide. Activated charcoal reduced more efficiently the phenolic compounds in SCW3 by 94.6%. Finally, when we pretreated SCW3 by activated charcoal and this was used for bioethanol production, 0.96 (%,w/v) bioethanol was produced and the ethanol yield based on reducing sugar reached 0.5.
Development of Glucoamylase & Simultaneous Saccharification and Fermentation Process for High-yield Bioethanol
Choi, Gi-Wook ; Han, Min-Hee ; Kim, Yule ;
KSBB Journal, volume 23, issue 6, 2008, Pages 499~503
The bioethanol for use as a liquid fuel by fermentation of renewable biomass as an alternative to petroleum is important from the viewpoint of global environmental protection. Recently, many scientists have attempted to increase the productivity of bioethanol process by developing specific microorganism as well as optimizing the process conditions. In the present study, which is based on our previous investigation on the pretreatment process, theproductivity of bioethanol obtained from simultaneous saccharification and fermentation (SSF) process was compared between various domestic materials including barley, brown rice, corn and sweet potato. Additionally, Solid glucoamylase (SGA; developed in Changhae Co.), from modified strain with UV, was used. The result was compared to commercial glucoamylase (GA). It was observed that the fermentation rate was increased together with the yield which can be derived from the final ethanol concentration. Especially, in the case of brown rice, compared to the experimental results using GA, the final ethanol concentration was 1.25 times higher and 18.4 g/L of the yield was increased. Also, the time required for reaching 95% of the maximum ethanol concentration is significantly reduced, which is approximately 36 hours, compared to 88 hours using GA. It means that SGA has excellent saccharogenic power.
Bioethanol Production using Endogenous Triticale Enzyme
Choi, Gi-Wook ; Kim, Yule ; Moon, Se-Kwon ;
KSBB Journal, volume 23, issue 6, 2008, Pages 504~508
The objectives of this study were to develope the economical process for bioethanol production from domestic triticale and investigate optimal fermentation conditions such as temperature, time, and enzyme concentration used to pre-treatment process. Triticale mash, containing 148 g of total sugar per 1 L of mash, was fermented with Saccharomyces cerevisiae CHY1011 at
. Fermentation of mash supplemented with enzyme was completed within 48-60 hours, and the ethanol yield was 410.9 L/tonne of dry base. On the other hand, fermentation of mash without enzyme addition was completed within 36-48 hours, but the ethanol yield was 342.2 L/tonne of dry base. For optimal bioethanol production from triticale, viscosity reduction enzyme was added in the pre-treatment process, and the fermentation rate of triticale was 92.0-94.2%. In addition, the results showed that bioethanol production of triticale by low-temperature pre-treatment would provide higher ethanol production efficiency and lower operating costs.
The Hydrolysis of Soybean Oil by Lipase Enzyme Catalyst
Lee, Jeong-Tae ; Kim, Eui-Yong ;
KSBB Journal, volume 23, issue 6, 2008, Pages 509~512
The hydrolysis reaction of soybean oil was conducted experimentally by various source enzymes. The analytical result of hydrolysate of soybean oil showed that the compositions were linoleic acid, olic acid, palmitic acid, and stearic acid in order. The enzymes CR-E and CC-E from Candida rufosa and Candida cylindracea had two hold or more hydrolysis conversions than those of Lipase 16, Novozyme 871, and Lipolase-100L under the same conditions. Therefore CR-E and CC-E were selected for further experiments. These two enzymes had similar ranges of optimun conditions as follows: pH 3-6,
, and water to soybean oil ratio of 3.3 or above. They finally got conversions 95% above.
Anti-wrinkle Activity of
-carotene Extracted & Purified from Recombinant Escherichia coli
Jo, Ji-Song ; Ku, Bo-Mi ; Kang, Sang-Soo ; Lee, Jae-Ran ; Kim, You-Geun ; Lee, He ; Kim, Sung-Bae ; Kim, Seon-Won ; Kim, Chang-Joon ; Chung, In-Young ;
KSBB Journal, volume 23, issue 6, 2008, Pages 513~518
This paper described the extraction/purification of
-carotene from recombinant E.coli and evaluation of anti-wrinkle activity of purified
-carotene. No significant differences in extraction yields were observed when hexane or isobutyl acetate was used. However, extraction from wet-cell cake resulted in 2-fold higher amount of
-carotene than that from dry cells. Disruption of 5 g-wet cells by ultrasonic homogenizer, acetone dehydration, extraction with isobutyl acetate resulted in 36 mg of
-carotene corresponding to 61.2% of recovery. The formation and separation of
-carotene crystal improved the purity. 633 mg of
-carotene crystal with 93% purity was obtained from 223 g/L of wet-cell cake harvested from 2.5-L fed-batch culture broth. The cultures of normal human primary fibroblast were performed to investigate the effect of
-carotene on cytotoxicity as MTT assay and anti-wrinkle activity as collagen synthesis assays.
-carotene was found to be optimal concentration at which 1.4-fold higher amount of collagen was synthesized than that in absence of
-carotene. This indicates that highly purified
-carotene can be obtained from recombinant E.coli by applying simple method with less toxic solvent and can be used in functional cosmetics as anti-wrinkle agent.
Enhanced production of monacolin-K through supplement of monacolin-K precursors into production medium and cloning of SAM synthetase gene (metK)
Lee, Mi-Jin ; Jeong, Yong-Seob ; Chun, Gie-Taek ;
KSBB Journal, volume 23, issue 6, 2008, Pages 519~524
Monacolin-K is a strong anti-hypercholesterolemic agent produced by Monascus sp. via polyketide pathway. High-yielding mutants of monacolin-K were developed through rational screening strategies adopted based on understanding of monacolin-K biosynthetic pathway. Through the experiments for investigating various amino acids as putative precursors for the monacolin-K biosynthesis, it was found that production level of monacolin-K was remarkably increased when optimum amount of cysteine was supplemented into the production medium. We suggested that these phenomena might be related to the special roles of SAM (S-adenosyl methionine), a putative methyl group donor in the biosynthetic pathway of monacolin-K, demonstrating close interrelationship between SAM-synthesizing primary metabolism and monacolin-K synthesizing secondary metabolism. Namely, increase in the intracellular amount of SAM derived from the putative precursor, cysteine which was extracellularly supplemented into the production medium might contribute to the significant enhancement in the monacolin-K biosynthetic capability of the highly mutated producers. On the basis of these assumptions derived from the above fermentation results, we decided to construct efficient expression vectors harboring SAM synthetase gene (metK) cloned from A. nidulans, with the hope that increased intracellular level of SAM could lead to further enhancement in the monacolin-K production through overcoming a rate-limiting step associated with monacolin-K biosynthesis. Hence, in order to overcome the plausible rate-limiting step associated with monacolin-K biosynthesis by increasing intracellular level of SAM, we transformed the producer mutants with an efficient expression vector harboring gpdA promoter of the producer microorganism, and metK gene. Notably, from the resulting various transformants, we were able to screen a very high-yielding transformant which showed approximately 3.3 fold higher monacolin-K productivity than the parallel nontransformed mutants in shake flask cultures performed under the identical fermentation conditions.
Cultivation of Sprout by Highly Concentrated Oxygen Water Soaking
Cha, Jin-Myeong ; Hong, Seung-Ho ; Kim, Sun-Yil ; Park, Ju-Young ; Kim, Maeng-Su ; Lee, In-Hwa ;
KSBB Journal, volume 23, issue 6, 2008, Pages 525~528
In order to compare the germination and growth rate of the sprouts soaked in highly concentrated oxygen water, it with specific amounts of oxygen dissolved was produced in a high pressure reactor by pressuring oxygen. The sprouts were observed after being soaked in
oxygen water with 20, 30, 40, 50 ppm of oxygen dissolved each. Results of ten days later indicate that the final germination rate of the sprout soaked in 50 ppm oxygen water was
higher than that of the sprout soaked in distilled water. The final growth length also measured 6-7 mm higher than the sprout soaked in distilled water, demonstrating that enough supply of oxygen to the sprout induces stability and efficiency in its growth.
Supercritical Extraction of Oriental Herb : Anti-aging and Anti-wrinkle Effects
Kim, In-Deok ; Kwon, Ryun-Hee ; Heo, Ye-Young ; Jung, Hye-Jin ; Kang, Hwan-Yul ; Ha, Bae-Jin ;
KSBB Journal, volume 23, issue 6, 2008, Pages 529~534
The reactive oxygen species generated by ultraviolet rays causes various types of cutaneous damage, such as lipid peroxidation and denaturation of the extra-cellular matrix. The accumulation of such damage contributes to skin aging, especially the formation of wrinkles. This study was carried out to develop functional cosmatic by using Oriental herb supercritical extracts (OHSE) for prevention of skin. Effects of OHSE on anti-oxidation, collagenase inhibition and collagen synthesis in normal human fibroblast were investigated. OHSE showed antioxidative activity as high as vitamin C, trolox and DL-penicillamine. Also OHSE showed promotive effect on collagen synthesis and inhibitory effect on collagenase activity. From this results, we conclude that OHSE may have the potential to be conveniently used as an additive in cosmetics for prevention and improvement of skin aging.
Study of Factors Influenced on denitrification in wastewater treatment
Jeong, Gwi-Taek ; Park, Seok-Hwan ; Park, Jae-Hee ; Bhang, Sung-Hun ; Lim, Eun-Tae ; Park, Don-Hee ;
KSBB Journal, volume 23, issue 6, 2008, Pages 535~540
In this study, the effects of several factors such as initial nitrate concentration, C/N ratio, biomass amount and external carbon source on denitrification process were investigated using synthetic wastewater and sludge obtained from wastewater treatment facility. As a result, the condition of lower initial nitrate concentration was increased to the removal rate of nitrate than that of high concentration. The increases of C/N ratio and initial biomass amount were linearly enhanced the removal rate. The use of ethanol as external carbon source was shown the highest removal yield than that of others.
Application of Advanced Treatment Process for Nitrogen Compounds Removal of Industrial Waste-water
Bhang, Sung-Hun ; Lim, Eun-Tae ; Jeong, Gwi-Taek ; Park, Jae-Hee ; Park, Seok-Hwan ; Kim, Seong-Jun ; Park, Don-Hee ;
KSBB Journal, volume 23, issue 6, 2008, Pages 541~545
This paper was studied to research regarding the removal of contained nitrogen in industrial wastewater which uses the A2O4 advanced water treatment process. The field researches of two companies' wastewater occurred in each wastewater treatment site to apply the A2O4 process system, it was observed them for 20 days. As a result of the A2O4 system advanced wastewater process which applied an altitude control process obtained
, and 20 mg/L total nitrogen compound concentration in the two wastewater plants. In conclusion, it applied the A2O4 system in the two companies' wastewater system.
Solubility and Storage Stability of Astaxanthin
Kim, So-Young ; Cho, Eun-Ah ; Yoo, Ji-Min ; In, Man-Jin ; Chae, Hee-Jeong ;
KSBB Journal, volume 23, issue 6, 2008, Pages 546~550
Basic characteristics of astaxanthin including solubility and stability were investigated. Astaxanthin showed a very poor solubility in water, but it was highly soluble in organic solvents such as acetone and acetic acid. The solubility of astaxanthin in acidic condition was 10-20 times higher than those in neutral and basic conditions. Astaxanthin was very unstable in acidic condition under UV irradiation and in the presence of oxygen. Also, heating even for a very short time accelerated the degradation of astaxanthin. In conclusion, it is required to enhance the water-solubility and stability of astaxanthin for industrial application in food and cosmetic area.
Effect of broccoli sprouts germination by soaking water condition
Park, Ju-Young ; Yoo, Chang-Hoon ; Lee, In-Hwa ; Hong, Seung-Ho ; Cha, Jin-Myeong ;
KSBB Journal, volume 23, issue 6, 2008, Pages 551~553
In order to optimization germination condition of broccoli, we carried out germination ratio experiment under soaking time, soaking temperature, oxygen concentration. The germination ratio results 98.5% following as soaking water having 10 ppm DO(demanded oxygen) during 4 hr at
. The result of provide the dissolved oxygen water, when the concentration of oxygen water were 7, 12 ppm, germination ratio were observed about 76%, 92%. Also, we showed the growth rate of 2 times in 12 ppm compared with 7 ppm. Consequently, germination ratio of broccoli increased cultivation condition at soaking water having high concentration oxygen more than supply to oxygen water of high concentration.
Expression of recombinant protein from Oenanthe javanica DC. leaf tissues as a biofactory
Shin, Dong-Il ; Park, Hee-Sung ;
KSBB Journal, volume 23, issue 6, 2008, Pages 554~556
Fresh Oenanthe javanica DC. leaves still attached to stem architecture were immersed in NaOH solution for 3 min before agroinfiltration and co-cultivation. MTT assay revealed that NaOH solution containing up to 0.7% was still safe for the leaf viability. Fluorometric GUS enzyme analysis showed that 0.5% NaOH-treated leaf tissues were efficiently transformed by vacuum infiltration for 20 min with Agrobacterium cells at a density of
to 1.0. These conditions worked well for the expression of HBsAg, which was confirmed by western blotting and ELISA.
Effect of surfactant on the micelle process for the pre-purification of paclitaxel
Jeon, Keum-Young ; Kim, Jin-Hyun ;
KSBB Journal, volume 23, issue 6, 2008, Pages 557~560
The micelle process was developed for pre-purifying paclitaxel from plant cell cultures of Taxus chinensis, giving a high purity and yield. The approach in this work was to transfer paclitaxel in the crude extract to an aqueous surfactant solution as a micelle, allowing organic solvents to be used for removal of lipids and non-polar impurities. In this work, the effects of various surfactants such as CPC, CTMAC, LTMAC, SDS, AOT, Tween, PEG, and Triton were examined on the yield, purity, and phase separation time in micelle process. Among these surfactants, CTMAC (5%, w/v) gave the best result in terms of paclitaxel yield (
), purity (
), and phase separation time (30 min). The use of micelles in the pre-purification process allows for rapid and efficient separation of paclitaxel from interfering compounds and dramatically increases the yield and purity of crude paclitaxel for subsequent purification steps.