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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 24, Issue 6 - Dec 2009
Volume 24, Issue 5 - Oct 2009
Volume 24, Issue 4 - Aug 2009
Volume 24, Issue 3 - Jun 2009
Volume 24, Issue 2 - Apr 2009
Volume 24, Issue 1 - Feb 2009
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Fabrication of Label-Free Biochips Based on Localized Surface Plasmon Resonance (LSPR) and Its Application to Biosensors
Kim, Do-Kyun ; Park, Tae-Jung ; Lee, Sang-Yup ;
KSBB Journal, volume 24, issue 1, 2009, Pages 1~8
In the past decade, we have observed rapid advances in the development of biochips in many fields including medical and environmental monitoring. Biochip experiments involve immobilizing a ligand on a solid substrate surface, and monitoring its interaction with an analyte in a sample solution. Metal nanoparticles can display extinction bands on their surfaces. These charge density oscillations are simply known as the localized surface plasmon resonance (LSPR). The high sensitivity of LSPR has been utilized to design biochips for the label-free detection of biomolecular interactions with various ligands. LSPR-based optical biochips and biosensors are easy to fabricate, and the apparatus cost for the evaluation of optical characteristics is lower than that for the conventional surface plasmon resonance apparatus. Furthermore, the operation procedure has become more convenient as it does not require labeling procedure. In this paper, we review the recent advances in LSPR research and also describe the LSPR-based optical biosensor constructed with a core-shell dielectric nanoparticle biochip for its application to label-free biomolecular detections such as antigen-antibody interaction.
The molecular mechanism of bacterial chemotaxis to environmental pollutants
Kim, Hye-Eun ; Kato, Junichi ; Lee, Sang-Ho ; Shim, Hyun-Woo ; Lee, Chang-Soo ;
KSBB Journal, volume 24, issue 1, 2009, Pages 9~16
Chemotaxis is the movement of an organism toward chemical attractant and away from chemical repellents. Several bacteria are known to cometabolically degrade some pollutants and attracted to the pollutants. The chemotactic responses to these compounds influence the efficiency of bioremediation because the first precondition of pollutant degradation is definitely confrontation between microorganisms and pollutants. In this review, we summarize present knowledge of about the chemotactic mechanism to environmental pollutants of Pseudomonas species.
Microbial Biosurfactants and the Treatment of Volatile Organic Compounds
Lee, Ki-Sup ; Kim, Gi-Eun ;
KSBB Journal, volume 24, issue 1, 2009, Pages 17~24
For the biosurfactant production process at first Candida bombicola, Sphingomonas yanoikuyae, Sphingomonas chungbukensis and Myxococcus flavescens were studied. As the most productive microorganisms C. bombicola, S. yanoikuyae and S. chungbukensis were selected. During many petrochemical industrial processes variable volatile organic componds are produced and they can cause an unpleasent and unhealthy atmosphere. Usually the volatile organic compounds are treated with chemical detergents. The chemical detergents cannot be easily degradable and can be accumulated in the nature. In this study we tried to develop a production process for the biosurfactants, which can substitute some chemical detergents in some chemical processes, with microorganisms. At second the treatment of the volatile organic compounds with the biosurfactants were tested and compared with the treatment with chemical detergent. The production productivities of the biosurfactant with microorganisms were compared. The growth patterns and kinetics of the microbial cells and the surface tension values of the biosurfactants were studied. The changes of the surface tension in variable pH conditions and sodium chloride concentrations were also studied. The volatile organic carbons were treated in a small plant scale. As the result of this study, it indicated that the specific growth rate of S. chungbukensis was the fastest by 0.144 (
). For surface tension, C. bombicola (38.1 dyne/cm) had the lowest value, and solubility of the volatile organic carbon was similar in C. bombicola and S. chungbukensis. (Toluene: about 0.1 Unit, Chloroform: about 0.6
0.7 Unit, Benzene: about 0.5
0.8 Unit). The biosurfactant, which were produced by C. bombicola, was selected for the further study for the volatile organic carbon treatment. With the biosurfactans from C. bombicola could remove the volatile organic carbon about 80% and this removal rate can be comparable with chemical detergent.
Biosynthesis of ceramide by deletion mutant of Saccharomyces cerevisiae
Kim, Se-Kyung ; Noh, Yong-Ho ; Yun, Hyun-Shik ;
KSBB Journal, volume 24, issue 1, 2009, Pages 25~29
Ceramide is important not only for the maintenance of the barrier function of the skin but also for the water-binding capacity of the stratum corneum. Though the effectiveness of ceramide is not understood fully, ceramide has become a widely used ingredient in cosmetic and pharmaceutical industries. However, ceramide production from Saccharomyces cerevisiae has not been widely studied and the quantity are very low. Gene deletion in the cell is used frequently to investigate the function of gene and verification research of drug target. Specially, deletion mutant library is useful for a large amount functional analysis of gene. In this study, deletion mutants of genes on the metabolic pathway of ceramide synthesis in S. cerevisiae were grown in a batch culture and the cellular content of ceramide was measured. The ceramide content was highest in
ydc1 mutant and 6 mg ceramide/g cell was obtained.
Statistical Optimization of Production Medium for Enhanced Production of Itaconic Acid Biosynthesized by Fungal Cells of Aspergillus terreus
Jang, Yong-Man ; Shin, Woo-Shik ; Lee, Do-Hoon ; Kim, Sang-Yong ; Park, Chul-Hwan ; Jeong, Yong-Seob ; Chun, Gie-Taek ;
KSBB Journal, volume 24, issue 1, 2009, Pages 30~40
Statistical optimization of the production medium was carried out in order to find an optimal medium composition in itaconic acid fermentation process. Itaconic acid utilized in the manufacture of various synthetic resins is a dicarboxylic acid biosynthesized by fungal cells of Aspergillus terreus in a branch of the TCA cycle via decarboxylation of cis-aconitate. Through OFAT (one factor at a time) experiments, six components (glucose, fructose, sucrose, soluble starch, soybean meal and cottonseed flour) were found to have significant effects on itaconic production among various carbon- and nitrogen-sources. Hence, using these six factors, interactive effects were investigated via fractional factorial design, showing that the initial concentrations of sucrose and cottonseed flour should be high for enhanced production of itaconic acid. Furthermore, through full factorial design (FFD) experiments, negative effects of
on itaconic acid biosynthesis were demonstrated, when excess amounts of the each component were initially added. Based on the FFD analysis, further statistical experiments were conducted along the steepest ascent path, followed by response surface method (RSM) in order to obtain optimal concentrations of the constituent nutrients. As a result, optimized concentrations of sucrose and cottonseed flour were found to be 90.4g/L and 53.8g/L respectively, with the corresponding production level of itaconic acid to be 4.36 g/L (about 7 fold higher productivity as compared to the previous production medium). From these experimental results, it was assumed that optimum ratio of the constituent carbon (sucrose) and nitrogen (cottonseed flour) sources was one of the most important factors for the enhanced production of itaconic acid.
Production and Analysis of Oxygenated Unsaturated Fatty Acids from Oleic Acid by Flavobacterium sp. Strain DS5
Song, Byung-Seob ; Han, Nam-Soo ; Lee, Bong-Hee ; Hou, Ching T. ; Kim, Beom-Soo ;
KSBB Journal, volume 24, issue 1, 2009, Pages 41~46
Vegetable oils are desirable inexpensive feedstocks for various bioproducts. The content of unsaturated fatty acids such as oleic and linoleic acids are 22% and 55% for soybean oil, 26% and 60% for corn oil, and 61% and 21% for canola oil, respectively. Keto and hydroxy fatty acids are useful industrial chemicals, used in plasticizer, surfactant, lubricant and detergent formulations because of their special chemical properties such as higher viscosity and reactivity compared with other fatty acids. In this study, a microbial isolate, Flavobacterium sp. strain DS5 (NRRL B-14859), was used to convert oleic acid to 10-ketostearic acid (10-KSA) via 10-hydroxystearic acid (10-HSA). Two bioconversion products, 10-KSA and 10-HSA, were quantitatively and qualitatively analyzed using gas chromatography, gas chromatography-mass spectrometry, and
-nuclear magnetic resonance. The maximum production of 10-KSA and 10-HSA in flask cultures were 3.4 g/L and 0.5 g/L, respectively. The optimum concentrations of glucose and yeast extract, addition time and volume of oleic acid for 10-KSA production were less than 20 g/L, more than 5 g/L, 18 hand 0.3 ml/50 ml, respectively.
Treatment of gas from the vent of a fine chemical plant using a pilot-scale biofilter
Ryu, Hee-Wook ; Lee, Tae-Ho ; Park, Chang-Ho ;
KSBB Journal, volume 24, issue 1, 2009, Pages 47~52
A pilot-plant biofilter (1750 mm W
2750 mm L
2000 mm H) packed with polyurethane foam (20 mm W
20 mm L
20 mm H) was installed in an fine chemical plant emitting gas streams containing ethyl alcohol, ethyl acetate, and dichloromethane. The biofilter was successfully operated for 30 days under highly fluctuating incoming concentrations (maximum 3500 ppm) at a residence time of 36 and 60 sec. Both ethyl alcohol and ethyl acetate were removed more than 95%, but dichloromethane removal was less than 50%. Malodor was also removed more than 90% from 17 days after start up.
The Effect of Microorganisms, Nutrients, and Surfactants on the Bioremediation of Oil-Contaminated Soil
Seon, Yong-Ho ;
KSBB Journal, volume 24, issue 1, 2009, Pages 53~58
This study was focused on the investigation of the characteristics of TPH and BTEX removal in oil-contaminated sandy soil and fine soil with injection of microorganisms, nutrients, and surfactants. As the result of the experiments maintained moisture contents by 10
20%, the TPH removal efficiency in oil-contaminated sandy soil was the highest in C-1 (microorganisms+nutrients), and the efficiency in C-2 (microorganisms+nutrients+surfactants) was higher than the efficiency in C-0(microorganisms). In 81 days, TPH removal efficiency in case of C-0, C-1 and C-2 showed 51%, 83%, 63% respectively. The results of D group with fine soil showed similar trends as C group, but the TPH removal efficiency of D group was lower than that of C group. Those of both C and D group were the highest in 1 group (microganisms+nutrients). The pH of fine soil was some lower than that of sandy soil or was similar to sandy soil. In 14 days, BTEX removal efficiency in case of C-0, C-1, C-2, D-0, D-1 and D-2 showed 99.8%, 99.4%, 96.0%, 99.5%, 99.2%, 96.3% respectively. Those of both C and D group were the highest in 0 group (microganisms).
Polysaccharide Characteristics from Hot Water Extract of Aloe saponaria Callus
Baek, Jin-Hong ; Kim, Myung-Uk ; Kang, Tae-Su ; Hur, Won ; Lee, Shin-Young ;
KSBB Journal, volume 24, issue 1, 2009, Pages 59~64
The callus formation from inferior leaf of Aloe saponaria was induced in M & S medium supplemented with 10-30
-naphthalene acetic acid) and 3-7
kinetin under incubation in the dark at
for 6 weeks. The hot water extract (
, 24 hrs) from cultured callus was obtained and the components analysis for the extract were examined to determine the callus can synthesized the bioactive component such as Aloe polysaccharide. The freeze dried extract contained the sugar of 53.2%, protein of 7.3%, ash of 18.5% and water of 21% (w/w). Two fractions (Fr-I and Fr-II) were obtained by Sepharose CL-4B gel permeation chromatography and Fr-I, major fraction was further purified with dialysis. From sugar analysis by TLC and GC, the purified Fr-I fraction consisted of glucose (77.6%), galactose (17.7%), mannose (4.7%, w/w) and uronic acid (trace). The molecular weight of purified Fr-I fraction determined by GPC was about 110 kDa.
Effect of Dissolved Oxygen on the Production of Epothilone in Bioreactor Cultures Sorangium cellulosum
Park, Su-Jeong ; Han, Se-Jong ; Kim, Byung-Woo ; Sim, Sang-Jun ;
KSBB Journal, volume 24, issue 1, 2009, Pages 65~69
The biological production of a potent anticancer agent, epothilone, by Sorangium cellulosum was carried out using flask and fermentor cultures. Soluble starch was selected as the main carbon source and the concentrations of lactose and yeast extract were optimized at 4 and 0 g/L, respectively, when using the flask cultures. In the fermentor cultures, the cells were cultivated at a high DO level of more than 80% of air saturation in the growth stage and then the DO level was controlled at about 50, 20 or 1-2% when the carbon source was exhausted. The epothilone production increased with decreasing DO level after the exhaustion of the carbon source, and the maximum concentration of epothilone was 5.4 mg/L. It was found that the DO level had significant regulation effects on the epothilone production.
Comparison of cellulolytic enzyme productivities in various semicontinuous culture modes of Trichoderma inhamatum KSJ1
Li, Hong-Xian ; Kim, Seong-Jun ;
KSBB Journal, volume 24, issue 1, 2009, Pages 70~74
For continuous culture of cellulolytic enzymes production to saccharify food wastes, refill concentration of Mandel's medium for continuous culture was 0.5%, and refill intervals were determined to 12 hours by analysis of COD and total nitrogen concentration after 4-days batch culture in flask level. As a result, amylase and FPase productivities were 3.5 and 1.0 U/L.hr, respectively. In 10 L bioreactor, the batch culture mode was compared with fed-batch, fill-and-draw for continuous production of cellulolytic enzyme. Enzyme productivities were most high at batch culture and followed by fed-batch culture. Amylase and FPase activities were 42.3 and 5.6 U/L.hr at batch culture, and 23.0, 2.8 U/L.hr at fed-batch culture, respectively. As a result, in continuous cultivation of cellulolytic enzymes by T. inhamatum KSJ1, the mode of fed-batch was most effective in 10 L bioreactor.
Influence of Organic, Inorganic Nitrogen Sources and Amino Acids on the Biosynthesis of Coenzyme
by Agrobacterium tumefaciens Mutant
Kim, Jeong-Keun ; Won, Yong-Bae ; Lee, Kang-Moon ; Koo, Yoon-Mo ;
KSBB Journal, volume 24, issue 1, 2009, Pages 75~79
The effect of inorganic, organic nitrogen sources and amino acids on the coenzyme
production and coenzyme
component ratio was investigated. Among the nine organic nitrogen sources, CSP showed a remarkable enhancing effect on the production of coenzyme
. But this enhancement was not observed in medium containing Bacto peptone, tryptone, casamino acid and soybean meal. These differences on the production of coenzyme
may be due to differences in kinds and amounts of component amino acids and peptides in the various organic nitrogen sources tested. In the addition of inorganic nitrogens, only
increase the coenzyme
production by 2.0 times compare to the control group. The addition of L-tyrosine to the medium containing Bacto tryptone, was also determined to be crucial for the coenzyme
production. But phenylalanin and tryptophan, other aromatic amino acids, had no stimulatory effect on the coenzyme
production. These results show that the production and components ratio of coenzyme
was greatly affected by the kinds and the concentration of inorganic, organic nitrogen sources as well as amino acids.
Purification of Recombinant CTP-Conjugated Human prostatic acid phosphatase for activation of Dendritic Cell
Yi, Ki-Wan ; Ryu, Kang ;
KSBB Journal, volume 24, issue 1, 2009, Pages 80~88
Human prostatic acid phosphatase (PAP), with comprehensive homology to glandular kallikrein, are representative serum biomarkers of prostate cancer. Dendritic cell (DC), which is the potent antigen-presenting cells(APC) in the immune system, can induce strong T cell responses against viruses, microbial pathogens, and tumors. Therefore, the immunization using DC loaded with tumor-associated antigens is a powerful method for inducing anti-tumor immunity. The CTP (Cytoplasmic Transduction Peptide) technology developed by Creagene which can transport attached bio-polymers like nucleic acids or proteins into the cell with high permeation efficiency. As the active forms of PAP can mediate apoptotic processing, we used multimer forms of PAP as an inactive form for antigen pulsing of DCs. In this study, multimeric forms of CTP-rhPAP was obtained according to the advanced purification process and subsequently confirmed by gel filtration chromatography, western blot and Dynamic Light Scattering. Therefore, CTP-conjugated PA multimers transduced into the cytoplasm were efficiently presented on the cell surface without any harm effect on cells via MHC class I molecules and result in induction of a large number of effector cell.
Performance of Pilot-Scale Biodiesel Production System
Jeong, Gwi-Taek ; Park, Jae-Hee ; Park, Seok-Hwan ; Park, Don-Hee ;
KSBB Journal, volume 24, issue 1, 2009, Pages 89~95
Biodiesel (fatty acid alkyl esters), which is produced from sustainable resources such as vegetable oil, animal fat and waste oils, have used to as substitutes for petro-diesel. In this study, we investigate the performance of 30 L and 300 L pilot-scale biodiesel production system using alkali-catalyst transesterification from soybean oil and rapeseed oil produced at Jeju island in Korea. The 30 L-scale biodiesel production was performed to in the condition of reaction temperature
, catalyst amount 1% (w/w) and oil to methanol molar ratio 1 : 8. At that reaction condition, the fatty acid methyl ester contents of product are above 98% within reaction time 30 min. Also, the conversion yield of over 98% was obtained in 300 L-scale biodiesel production system using rapeseed oil and soybean oil. The quality of biodiesel produced from reaction system was satisfied to recommended quality standard of Korea. Our results may provide useful information with regard to the scale-up of more economic and efficient biodiesel production process.
A study on the effect of CspA expression on the productivity of recombinant protein at low temperature
Kim, Su-Hyun ; Heo, Mi-Ae ; Lee, Sun-Gu ;
KSBB Journal, volume 24, issue 1, 2009, Pages 96~100
One of the major drawbacks associated with the high-level expression of the recombinant proteins in Escherichia coli is the formation of insoluble inclusion bodies in the cytoplasm. Production of recombinant protein at reduced temperature has proven effective in improving the solubility of a number of structurally and functionally unrelated proteins, but a major limitation of using low temperatures for recombinant protein production in E. coli is the reduced rate of synthesis of the heterologous protein caused by the significant reduction of cell growth rate. Here we investigated the effect of co-expression of CspA, a cold-shock protein known to be RNA chaperone at low temperature, on the productivity of recombinant protein at various temperatures by using green fluorescence protein (GFP) as a model recombinant protein. We could observe that the co-expression of CspA enhanced the productivity of GFP at
by accelerating the growth of E. coli at the temperature. On the other hand, the CspA coexpression didn't affect the cell growth rate as well as the specific GFP production rate at other tested temperatures,
Effect of Rhus verniciflua Stokes Extract on the Alcohol-Metabolizing Enzyme Activities
Yoo, Gui-Jae ; Kim, So-Young ; Choi, A-Reum ; Son, Min-Hee ; Kim, Dong-Chung ; Chae, Hee-Jeong ;
KSBB Journal, volume 24, issue 1, 2009, Pages 101~105
Alcohol oxidation activities and optimization of extraction conditions of Rrhus verniciflua Stokes (RVS) extract were evaluated for the development of a functional biomaterial for improving liver function. When alcohol oxidation activities of RVS was analyzed, the Rrhus verniciflua Stokes bark (RVSB) were higher than the Rrhus verniciflua Stokes heartwood (RVSH). Alcohol oxidation activity value of RVSB increased in a concentration-dependent manner. In the comparative analysis between Hovenia dulcis Thunb (HOT) and Alnus japonica Steud (AJS) which was reported as a alcohol oxidation material, alcohol oxidation activity is much higher than the others. The experimental conditions were optimized for alcohol oxidation-active components production from RVSB. The extraction conditions such as temperature, time, pH and particle size were performed. It was recommended to extract the alcohol oxidation-active components from RVSB by hot water (pH 7.0) at
for 8 hours.
On-line monitoring of microorganism cultivation processes using optical sensing membranes for simultaneous detection of dissolved oxygen and pH
Kim, Chun-Kwang ; Rhee, Jong-II ;
KSBB Journal, volume 24, issue 1, 2009, Pages 106~112
An optical sensing membrane has been fabricated to measure the concentration of dissolved oxygen(DO) and pH value simultaneously. It has employed HPTS as a pH sensitive dye and a ruthenium(II) complex as a DO sensitive dye. The sensing membrane has been applied to wells in a 24-well microtiter plate. Using the 24-well microtiter plate the concentrations of dissolved oxygen and pH values have been on-line monitored during the cultivations of E.coli DH5
, B.cereus 318 and P.pastoris X-33. On-line monitoring of DO and pH in microorganism cultivation processes showed good performance of the sensing membrane containing 5 mM HPTS and 2 or 5 mg/mL Rudpp.