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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 24, Issue 6 - Dec 2009
Volume 24, Issue 5 - Oct 2009
Volume 24, Issue 4 - Aug 2009
Volume 24, Issue 3 - Jun 2009
Volume 24, Issue 2 - Apr 2009
Volume 24, Issue 1 - Feb 2009
Selecting the target year
Regulation of cellular functions of p53 by ubiquitination
Jung, Jin-Hyuk ; Lee, Joon-Young ; Lee, Sun-Mi ; Choe, Tae-Boo ; An, Sung-Kwan ;
KSBB Journal, volume 24, issue 3, 2009, Pages 217~226
p53 undergoes various post-translational modifications, including phosphorylation, ubiquitination, sumoylation, acetylation, methylation, and poly(ADP-ribosyl)ation. Modification of p53 widely affects to various functions of p53. Acetylation and phosphorylation of p53 have been studied for regulating its transcriptional activity which is observed in various stress condition. Otherwise, ubiquitination of p53 by Mdm2 has been well-studied as a canonical ubiquitin-mediated proteasomal degradation pathway. Moreover several investigators have recently reported that ubiquitination of p53 modulates not only its proteasome-dependent degradation by poly-ubiquitination but also its localization and transcriptional activity by mono-ubiquitination which usually does not serve the proteasome dependent degradation. Here we review recent studies on the cellular functions of p53 regulated by post-translational modifications, particularly focusing on mechanisms of ubiquitination.
Biosensor System for the Detection of Agrichemicals and Its Applications
Park, Tae-Jung ; Yang, Min-Ho ; Lee, Sang-Yup ; Kim, Soo-Hyun ;
KSBB Journal, volume 24, issue 3, 2009, Pages 227~238
In the recent years, some organic toxic chemicals were used for obtaining high-yield productivity in agriculture. The undegraded pesticides may remain in the agricultural foods through atmosphere, water, and soil and cause public health problems to environmental resources and human beings even at very low concentrations. Small amounts of pesticides can affect a central nervous system, resulting in immunogenic diseases, infertility problems, respiratory diseases and born marrow diseases, which can lead even to death. Monitoring of the environmental pesticide is one of the important issues for the human well-being. Several kinds of biosensors have been successfully applied to the detection of agrichemical toxicity. Also, few platforms for biocide detection have been definitely developed for the degradation and reaction of pesticides. Biochip and electrochemistry experiments involve immobilizing a receptor molecule on a solid substrate surface, and monitoring its interaction with an analyze in a sample solution. Furthermore, nanotechnology can be applied to make high-throughput analyses that are smaller, faster and sensitive than conventional assays. Some nanomaterials or nanofabricated surfaces can be coupled to biomolecules and used in antibody-based assays and enzymatic methods for pesticide residues. The operation procedure has become more convenient as it does not require labeling procedure. In this paper, we review the recent advances in agrichemical defection research and also describe the label-free biosensor for pesticides using various useful detection methods.
Microbial styrene monooxygenase-catalyzed asymmetric synthesis of enantiopure styrene oxide derivatives
Lee, Eun-Yeol ; Park, Sung-Hoon ;
KSBB Journal, volume 24, issue 3, 2009, Pages 239~245
Enantiopure styrene oxide derivatives are versatile building blocks for the synthesis of enantiopure pharmaceuticals. Styrene monooxygenase (SMO) catalyzes an asymmetric addition of an oxygen atom into a double bond of vinylaromatic compounds. SMO is a commercially potential biocatalyst to synthesize a variety of enantiopure epoxides with high enantiopurity and recovery yield. In this paper development of SMO biocatalyst and commercial feasibility of SMO-catalyzed asymmetric synthesis of enantiopure stylers oxide derivatives are reviewed.
Effects of Proline and Gelatin on hCTLA4Ig Production in Transgenic Rice Suspension Cells
Song, Mi-Na ; Cheon, Su-Hwan ; Kwon, Jun-Young ; Choi, Sung-Hun ; Kim, Dong-Il ;
KSBB Journal, volume 24, issue 3, 2009, Pages 246~252
Rice cells were transformed to express human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) using RAmy3D promoter. hCTLA4Ig was produced and secreted into culture media inducibly when sugar was depleted. The obstacles of this system are the cell death and release of proteases by sugar starvation. These problems resulted in the losses of stability and productivity of hCTLA4Ig. Therefore, the effects of proline as an inhibitor of cell death were investigated. When 4 mM proline was added in sugar-free media, the cell death and release of proteases were reduced. As a consequence, the production of hCTLA4Ig was enhanced. In addition, the effects of protein stabilizers such as gelling agents were studied. It was found that the application of 0.01 g/L gelatin led to an increase in hCTLA4Ig production. This increase might be originated from the stabilization of hCTLA4Ig. In conclusion, the production of hCTLA4Ig could be enhanced by the additions of proline and gelatin in transgenic rice cell cultures.
Optimization of Fabrication Conditions for Immunosensor Strip to Detect Escherichia coli O157 : H7
Park, So-Jung ; Kim, Young-Kee ;
KSBB Journal, volume 24, issue 3, 2009, Pages 253~258
In this study, the optimization of fabrication conditions was accomplished to make immuno-strip biosensor by the combination of enzyme linked immunosorbent assay (ELISA) and immuno-chromatographic strip techniques for the detection of Escherichia coli O157 : H7. Optimal fabrication conditions of capture antibody concentration, detection antibody concentration, and additive composition of running buffer solution were determined. Optimal concentration was determined as 1.0 mg/mL for both of capture antibody and detection antibody. A composition of 0.5% Tween20 and 3% BSA were selected as optimal additive for buffer solution to prevent non-specific binding.
Functional Analysis of an Antibiotic Regulatory Gene, afsR2 in S. lividans through DNA microarray System
Kim, Chang-Young ; Noh, Jun-Hee ; Lee, Han-Na ; Kim, Eung-Soo ;
KSBB Journal, volume 24, issue 3, 2009, Pages 259~266
AfsR2 in Streptomyces lividans, a 63-amino acid protein with limited sequence homology to Streptomyces sigma factors, has been known for a global regulatory protein stimulating multiple antibiotic biosynthetic pathways. Although the detailed regulatory mechanism of AfsK-AfsR-AfsR2 system has been well characterized, very little information about the AfsR2-dependent down-stream regulatory genes were characterized. Recently, the null mutant of afsS in S. coelicolor (the identical ortholog of afsR2) has been characterized through DNA microarray system, revealing that afsS deletion regulated several genes involved in antibiotic biosynthesis as well as phosphate-starvation. Through comparative DNA microarray analysis of afsR2-overexpressed S. lividans, here we also identify several afsR2-dependent genes involved in phosphate starvation, morphological differentiation, and antibiotic regulation in S. lividans, confirming that the AfsR2 plays an important pleiotrophic regulatory role in Streptomyces species.
Behaviors of Glucose Decomposition during Dilute-Acid Hydrolysis of Lignocellulosic Biomass
Jeong, Tae-Su ; Oh, Kyeong-Keun ;
KSBB Journal, volume 24, issue 3, 2009, Pages 267~272
During a dilute acid hydrolysis, degradation products are formed or liberated by pre-treatment of lignocelluloses depend on both the biomass and the pretreatment conditions such as temperature, time, pressure, pH, redox conditions, and addition of catalysts. In lignocellulosic biomass, sugars can be degraded to furfural which is formed from pentoses and 5-hydroxymethulfurfural (HMF) from hexoses. 5-HMF can be further degraded, forming levulinic acid and formic acid. Acetate is liberated from hemicellulose during hydrolysis. Some decomposed compounds hinder the subsequent bioconversion of the solubilized sugars into desired products, reducing conversion yields and rates during fermentation. In the present work, samples of rapeseed strawwere hydrolyzed to study the optimal pretreatment condition by assessing yields of sugars and decomposed products obtained under different reaction conditions (
0.5-1.25% (w/w), reaction time 0-20 min and temperature range 150-220 C). A careful analytical investigation of acid hydrolyzate of rapeseed straw has not yet been undertaken, and a well-closed mass balance for the hydrolyzate in general is necessary to verify the productivity and economic predictions for this process.
Antibacterial Activity against Food-poisoning Causing Bacteria and Characterization of Lactobacillus plantarum YK-9 Isolated from Kimchi
Song, You-Jin ; Park, Su-Ho ; You, Ji-Young ; Cho, Yun-Seok ; Oh, Kye-Heon ;
KSBB Journal, volume 24, issue 3, 2009, Pages 273~278
The purpose of this work was to investigate the antibacterial activity of Lactobacillus plantarum YK-9 isolated from fermented Kimchi. Morphological and biochemical characteristics of L. plantarum YK-9 were examined. Phylogenetic analysis using 16S rRNA sequencing was performed to identify the strain, and the strain could be assigned to Lactobacillus plantarum, designated as L. plantarum YK-9. The strain was registered in GenBank as [FJ669130]. During the incubation period of L. plantarum YK-9, the changes of bacterial growth and residual organic acids were monitored. HPLC was used to confirm the organic acids produced in the cultures as metabolites. L. plantarum YK-9 produced both lactic acid and acetic acid, which were responsible for the pH decrease during growth. Initial pH 7.0 of the cultures decreased to 3.6 at the incubation after 72 hours, and concentrations of lactic acid and acetic acid increased to approximately 588.7 mM and 255.5 mM, respectively. The antibacterial activities against food-poisoning causing bacteria were examined with 20-fold concentrated culture supernatants from L. plantarum YK-9, and the antibacterial effects were clearly observed against all the bacteria tested in this work.
Inhibition of Oligomycin Biosynthesis by olmA5 Gene Knock-out in Streptomyces avermitilis
Kang, Hyun-Woo ; Ryu, Yeon-Woo ;
KSBB Journal, volume 24, issue 3, 2009, Pages 279~286
Streptomyces is well known for their ability to synthesize enormous varieties of antibiotics as secondary metabolites. Among them, S. avermitilis produces avermectins, a group of antiparasitic agents used in human and veterinary medicine. However, S. avermitilis also produces oligomycin, which is a potential toxic inhibitor of oxidative phosphorylation in mammalian cells. Therefore, we decided to disrupt oligomycin synthetase gene to prevent co-production of oligomycin in S. avermitilis. To create plasmid for disruption, the smallest gene of oligomycin synthetase gene cluster was obtained by PCR from S. avermitilis chromosome. Then, apramycin resistance gene was inserted in oligomycin synthetase gene for selection. After transformation of this plasmid, oligomycin synthetase gene (olmA5) in the chromosome was displaced with disruption cassette on the plasmid via homologous recombination. As a result of this gene replacement, we obtained mutants (olmA5::apra) that no longer makes the toxic oligomycin. And the mutants confirmed by PCR and HPLC analysis. However, showed no increasement of avermectin production in the mutant was observed.
Quantitative Relationship Analysis of Bacterial Metabolic Network using ARACNE
Nguyen, Thuy Vu An ; Hong, Soon-Ho ;
KSBB Journal, volume 24, issue 3, 2009, Pages 287~290
Metabolic network is composed of more than thousands of metabolic reactions. Therefore, understanding of metabolic behavior of microorganisms is required to engineer metabolism of microorganisms. In this paper, we employed ARACNE (Algorithm for the Reconstruction of Accurate Cellular Networks) to quantify relationships among metabolic subpathways. The results showed that ARACNE analysis can give new insight into the study of bacterial metabolism.
Practical Demonstration of YPNR Process to Elimination the Total Nitrogen Ingredient in Sewage
Lim, Eun-Tae ; Jeong, Gwi-Taek ; Bhang, Sung-Hun ; Kim, Yong-Un ; Park, Jae-Hee ; Park, Seok-Hwan ; Park, Don-Hee ;
KSBB Journal, volume 24, issue 3, 2009, Pages 291~295
This study performed verification experiment for the removal of total nitrogen in sewage from a Town M village sewage treatment plant using YPNR processes. The total nitrogen discharged after the denitrification process was maintained at a level of 8-15 mg/L, which results in the total nitrogen removal efficiency above 68% on average. The total nitrogen components in discharged water consisted of 16% of ammonia nitrogen, 6% of nitrite nitrogen, and 77% of nitrate nitrogen, which reaches a 95% nitrification efficiency. Hence, the YPNR advanced treatment process used in this study can be successfully applied to sewage treatment.
Effect of Factors of Nitrification Process in Wastewater Treatment
Jeong, Gwi-Taek ; Park, Seok-Hwan ; Park, Jae-Hee ; Lim, Eun-Tae ; Bang, Sung-Hun ; Park, Don-Hee ;
KSBB Journal, volume 24, issue 3, 2009, Pages 296~302
This paper was investigated the research regarding the effects of several factors such as initial ammonium nitrogen concentration, aeration rate. biomass amount and C/N ratio on nitrification process using synthetic wastewater and activated sludge obtained from wastewater treatment facility. As a result, in high ammonium nitrogen concentration above 100 mg/L, the pH of wastewater was dropped to pH 6.8. The increases of initial ammonium nitrogen concentration, aeration rate and initial biomass amount were linearly enhanced the removal rate of ammonium nitrogen. In the condition of C/N ratio of 0 to 3, high ammonium nitrogen removal rate was obtained.
Scaling Up Study of Exopolysaccharide Production through Mycelial Submerged Cultivation of Ganoderma lucidum
Lee, Hak-Su ; Lee, Shin-Young ;
KSBB Journal, volume 24, issue 3, 2009, Pages 303~311
A scaling up study for the exopolysaccharide (EPS) production by submerged culture of Ganoderma lucidum was carried out in jar fermenter systems (2.6, 20 and 75 L) under bi-staged pH process. Profiles of dissolved oxygen (DO) and volumetric coefficient of oxygen transfer (
) as a function of operating variables (agitation speed and aeration rate) was investigated, and a correlation between
and operating variables was analysed statistically. Under bi-staged pH process, no limitation of DO was observed at agitation speeds tested in the range of 200 and 600 rpm, and the highest EPS production was obtained at the level of DO of
. From the regression analysis, the relation between
, gas velocity (Vs), stirrer speed (N) and impeller diameter (Di) could be expressed as :
It was found that under 2.6 L jar fermenter, the optimum agitation speed and aeration rate was 400 rpm and 1 vvm, respectively, obtaining the EPS production of 15.43 g/L. Under the submerged cultivation of G. lucidum in jar fermenters of
, the similar EPS yields at each fermenter were achieved during scaling up based on
value for maximum EPS production was
Development of hypothermic preservation solution for the human dermal fibroblast using protein hydrolysates
Byoun, Soon-Hwi ; Choe, Tae-Boo ;
KSBB Journal, volume 24, issue 3, 2009, Pages 312~320
Stable cell preservation is an essential factor in the regenerative medicine for cell therapies and transplantation of biologic materials. In this study, we studied to provide more stable hypothermic preservation by protection of cell damage during the preservation at
. The result of searching for key components that have excellent efficacy in hypothermic preservation of cells, we have identified the fact that the hypothermic preservation adding protein hydrolysates such as yeast hydrolysate is far superior to others. All protein hydrolysates that are derived from animal, plant and microbe sources have superior efficacy, especially the peptides which have molecular weights under 10 kDa have the best efficacy among the components of protein hydrolysate. The protein hydrolysates prevented the decrease of ATP level in the cells caused by hypothermic environment and they inhibited the generation of ROS. Adding antioxidants and control agents of osmotic pressure were showed to have more superior efficacy in hypothermic preservation. Finally, KUL261 solution (DMEM/F12 1 : 1 medium, yeastolate 1%,
, dextran 2.5%), the preservation solution developed in this study, showed the best efficacy in both cell viability and cell growth more than other conventional preservation solutions. In conclusion, the improved hypothermic preservation solution that contains the protein hydrolysates as a key component provide the best preservation efficacy. It provides better efficacy than other preservation solutions and will contribute to both the development of regenerative medicine and global commercialization in this therapeutic field.
Optimization of Medium for Astaxanthin Production by Paracoccus sp. Using Response Surface Methodology
Choi, Jong-Il ; Lee, Hee-Sub ; Choi, Seon-Kang ; Kim, Jae-Hun ; Kim, Jin-Kyu ; Misawa, Norihiko ; Byun, Myung-Woo ; Lee, Ju-Woon ;
KSBB Journal, volume 24, issue 3, 2009, Pages 321~326
This study was to optimize the medium components for astaxanthin production in Paracoccus sp. through surface response methodology. A screening test was first conducted on 5 medium components using a Plackett-Burman design, from which
and yeast extract were identified as the significant factors affecting astaxanthin production. These significant factors were optimized by central composite design of experiments and response surface methodology, as 2.83 g/L
and 7.02 g/L yeast extract, respectively. The expected astaxanthin concentration with these optimized medium compositions were 0.925 mg/L. In flask culture, the experimentally obtained concentration of astaxantin was 1.021 mg/L, where it had been 0.4 mg/L before optimization.