Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal Basic Information
Journal DOI :
Korean Society for Biotechnology and Bioengineering
Editor in Chief :
Volume & Issues
Volume 24, Issue 6 - Dec 2009
Volume 24, Issue 5 - Oct 2009
Volume 24, Issue 4 - Aug 2009
Volume 24, Issue 3 - Jun 2009
Volume 24, Issue 2 - Apr 2009
Volume 24, Issue 1 - Feb 2009
Selecting the target year
Antioxidant and Anti-inflammatory Activity of Ethanol Extract of Malus micromalus Makino in Jeju Island
Lee, Ju-Yeop ; Kang, Min-Chul ; Lee, Jung-A ; Ko, Kwang-Hyo ; Kim, Bong-Seok ; Han, Jong-Heon ; Kim, Se-Jae ; Kim, Gi-Ok ;
KSBB Journal, volume 24, issue 4, 2009, Pages 327~333
The antioxidant and anti-inflammatory activities of ethanol extract of Malus micromalus were studied in vitro. Ethanol extract of M. micromalus showed scavenging effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide (NO) radicals. In addition, ethanol extract of M. micromalus inhibited the generation of superoxide anion (
) radical and uric acid by xanthine oxidase. We also investigated the effect of ethanol extract of M. micromalus on NO production in a lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells. Ethanol extract of M. micromalus significantly inhibited NO production and this inhibitory action was not due to the cytotoxicity. The expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was markedly down-regulated by ethanol extract of M. micromalus. These results indicate that the inhibitory action of ethanol extract of M. micromalus on NO production in LPS-stimulated macropages might be due in part to abrogation of iNOS and COX-2 protein induction. Taken together, this study suggests that ethanol extract of M. micromalus could contribute to the chemoprevention and therapy of oxidative stress and inflammation.
Isolation and Characterization of Plant Growth Promoting Rhizobacterium Bacillus subtilis YK-5 from Soil
Yeo, Soo-Hwan ; Yook, Young-Min ; Kim, Hyun-Soo ;
KSBB Journal, volume 24, issue 4, 2009, Pages 334~340
For the isolation of a plant growth-promoting rhizobacteria, strain YK-5 was selected from approximately 400 thermostable strains isolated from special soil samples. Strain YK-5 produced an antifungal compound, and optimum carbon and nitrogen sources for the production of the antifungal compound were investigated against Aspergillus flavus as a test strain. Modified LB medium containing 1% peptone, 1% yeast extract and 5% black sugar was determined to be the optimal medium for growth and antifungal compound production. Culture broth of strain YK-5 potently inhibited growth of the phytopathogenic fungus Fusarium oxysporum KACC 40052 for 7 days. The plant growth-promotion function of strain YK-5 was tested against radish and rice in pot trials. Leaf number, plant height and root length in YK-5-treated radish markedly exceeded (> 60%) those of untreated radish. Leaf length and white rootlet development were markedly more prominent than in commercially-treated rice plants. Strain YK-5 was determined to be Bacillus subtilis YK-5 by physiological, chemotaxonomical, and phylogenetical analyses.
Functional Expression of Candida antarctica Lipase A in Pichia a pastoris and Escherichia coli
Park, Hye-Jung ; Kim, Yong-Hwan ;
KSBB Journal, volume 24, issue 4, 2009, Pages 341~346
Candida Antarctica lipase A (CalA) has been used because of its suitability in industrial applications. CalA has unique features capable to accept tertiary and sterically hindered alcohols among many hydrolases. CalA gene was cloned and constructed in expression vector such as pColdIII/CalA and
/CalA. The gene encoding pColdIII/CalA was functionally expressed in the cytoplasm of Escherichia coli
B (DE3) cells. The plasmid
/CalA linearized by BstX I was integrated into 5'AOX1 region of the chromosomal DNA and was functionally expressed in the methyl atrophic yeast Pichia pastoris. Expressed CalA in P. pastoris (0.7 Unit/mL) showed 35 times higher activity than that in E. coli expression system (0.02 Unit/mL).
Preservation of Cosmetics by Ethanol Extract of Scutellaria baicalensis
Hwang, Shin-Hye ; Park, Chang-Ho ;
KSBB Journal, volume 24, issue 4, 2009, Pages 347~352
Ethanol extract (1.0 wt%) of Scutellaria baicalensis
practically satisfied CTFA (The cosmetics, Toiletry, and Fragrance Association) standard in its antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans which were inoculated in skin toner and skin lotion. At concentrations of the extract less than 1.0 wt% the survival rate was the best for S. aureus and the worst for P. aeruginosa. The antimicrobial effect seems to be due to the damage to the bacterial cell wall as evidenced by the images of a Field Emission-Scanning Electron Microscope. The extract also showed a high anti-oxidant effect, and superoxide dismutase (SOD)-like activity reached 80% at 1,000 ppm. These findings suggest that the extract of Scutellaria baicalensis
is applicable to cosmetics as a natural preservative and an anti-oxidant.
The Removal of Organics, Nitrogen and Phosphorus in Loop Reactor Using Fluidized Media
Seon, Yong-Ho ;
KSBB Journal, volume 24, issue 4, 2009, Pages 353~360
This study was accomplished using Anaerobic/Anoxic/Oxic upflow packed-bed column reactors with fixed media and Loop Reactor with fluidized media instead of Oxic reactor. The objectives of this study was to investigate the characteristics of organics, nitrogen and phosphorus removal from sewage with the HRT. The average removal efficiencies of
and SS increase as increasing the hydraulic retention time (HRT) until 16 h of the HRT, and they were constant over 16 h of the HRT. The removal efficiency of
in case of packed-bed reactor and Loop Reactor was about 86.6% and 90.9% respectively at 16 h of the HRT. The removal efficiency of SS in packed-bed reactor and Loop Reactor was about 78.0% and 88.2% respectively at 16 h of the HRT. The average removal efficiencies of
showed similar trends as those of
and SS. At the HRT of 16 h, the removal efficiency of
in case of packed-bed reactor and Loop Reactor was 63.5%, 75.2% and that of
was 60.7%, 73.6% respectively. The average removal efficiencies of T-N and T-P increase as increasing the HRT. The removal efficiencies of T-N and T-P in Loop Reactor were 33.6% and 54.5% respectively at 16 h of the HRT and T-N and T-P were better removed in Loop Reactor. From this result, it was found that the performance of Loop Reactor was much higher than the performance of packed-bed reactor and the optimum HRT was 16 h.
Optimization the Xylose Fractionation Conditions of Pepper Stem with Dilute Sulfuric Acid
Won, Kyung-Yoen ; Oh, Kyeong-Keun ;
KSBB Journal, volume 24, issue 4, 2009, Pages 361~366
Response surface methodology (RSM) was used for optimization the fraction conditions of xylose from pepper stem with dilute sulfuric acid. The independent variables were acid concentration in the range of 1.134 to 2.866%, reaction temperatures in the range of 142.68 to
, and hydrolysis time in the range of 6.34 to 23.66 min. were studied. The dependent variables were xylose yield from pepper stem, and the production of by-products, for example, furfural, acetic aicd, HMF etc. Experimental results had a good match with statistical result. The maximum xylose yield obtained in this experiment was 71% concentration.
Extraction of Oil from Canola Seeds with Supercritical Carbon Dioxide
Hwang, Ah-Reum ; Jung, In-Il ; Lim, Gio-Bin ; Ryu, Jong-Hoon ;
KSBB Journal, volume 24, issue 4, 2009, Pages 367~376
In this study, two supercritical extraction systems of different scale, analytical-scale and lab-scale, were employed to investigate the extraction efficiency of canola oil from canola seeds using supercritical carbon dioxide (
) as an extraction solvent. The effects of various parameters such as extraction temperature (
), pressure (200~500 bar), particle size, and
flow direction on the extraction rate and yield were examined in detail. Triglycerides and fatty acids in the extracted canola oil were analyzed quantitatively by high-performance liquid chromatography and gas chromatography. The solubility values of canola oil in
could be calculated from the experimental results. Similar extraction yields were obtained from both analytical-scale and lab-scale extraction systems. The extraction rates obtained under solvent (
) upflow conditions were found to be higher than those of solvent downflow extraction. However, the effect of
flow direction on the extraction yield was observed to be relatively insignificant.
Cell Growth and Lipid Production from Fed-batch Cultivation of Chlorella minutissima according to Culture Conditions
Oh, Sung-Ho ; Han, Jae-Gun ; Kim, Na-Young ; Cho, Jeong-Sub ; Yim, Tae-Bin ; Lee, Shin-Young ; Lee, Hyeon-Yong ;
KSBB Journal, volume 24, issue 4, 2009, Pages 377~382
The culture condition of growing Chlorella minutissima was optimized to produce biodiesel for fed-batch cultivation. First, under heterotrophic cultivation, the optimum level of glucose was determined to be 10 g/L for 20 days. After, three cultivation conditions were operated: autotrophic, heterotrophic, and mixotrophic growth. The lipid level and the maximum cell concentration from the fed-batch heterotrophic process were 32.0 (%, v/v) and 15.0 (g-dry wt./L) in 20 L flask, respectively. In addition, since the relatively constant specific lipid production rate was observed as 0.040 (% lipid/g-dry wt./day) at the latter period of cultivation time, the fed-batch process could maintain continuous lipid production. Fed-batch process is higher than those values from the batch process. The lipids from the fed-batch process contained over 38% of
, known as the suitable composition for the biodiesel application. For mixotrophic and heterotrophic growth under fed-batch condition, glucose was proved to be an appropriate carbon source for a large scale outdoor cultivation. For fed-batch cultivation, the feeding rate of seawater medium containing glucose was decided to be 0.5 L/day. The mixotrophic cultivation maintained maximum cell concentration of 24 (g-dry wt./L) and the lipid level of 43 (%, w/w). The lipid composition from this process was also proved to be suitable for the biodiesel production. The fatty acids from the mixotrophic growth contains 18% of
and 49% of
, implying It also tells that C. minutissima is a suitable resource of biodiesel. Especially, the mixotrophic cultivation with fed-batch process might be useful for the large scale cultivation for the biodiesel production.
Studies of Xanthium strumarium Extract Suppressing Th17-cell Differentiation and Anti-dermatitic Effect in BMAC-induced Atopy Dermatitis of NC/Nga Mice
Kim, Kum-Lan ; Choe, Tae-Boo ;
KSBB Journal, volume 24, issue 4, 2009, Pages 383~392
Xanthii fructus which is well known as "Chang-ihjah" in Korea is the dried fruit of Xanthium strumarium L. (or Xanthium sibiricum PATR. Ex WIDD., Asteraceae. XS). Water extract of this fruit has been used for treatment of various inflammatory diseases such as tympanitis, allergic rhinitis, or ozena as alternative therapy material usually by oral administration in far Eastern countries including Korea. In this study, the effect of XS extract (XS-E) or XS-30% acetone fraction layer (XS-30% AFL) on the differentiation of
T cells isolated from NC/Nga mouse and the production of IL-17 was investigated. The experimental results showed that
/mL of XS-E could decrease the production of IL-17 by
Th17 cells by 2 fold and only
/mL of XS-30% AFL could inhibit 3.5 fold. The amount of IL-17A and IL-22 mRNA determined by real-time PCR was decreased remarkably when XS-E or XS-30% AFL was treated on
Th17 cells(p<0.01, p<0.001). The amount of IL-17A protein determined by ELISA was also decreased remarkably(p<0.05, p<0.001). To study the effect of XS-E or XS-30% AFL on the proliferation of Th17 cells,
T cells of a NC/Nga mouse was firstly differentiated by rIL-6/TGF-
and then stimulated by rIL-23. The control group of Th17 cells were doubled every each day, while those of XS-E or XS-30% AFL treated group were shown to be delayed remarkably by these extracts. In conclusion, XS can inhibit the differentiation of Th17 cells of NC/Nga mouse and the production of IL-17 successfully, which may be a beneficial result for the treatment of atopic skin dermatitis.
Development of Protein Secretion System using Type III Secretion System of Salmonella
Dinh, Le Tam Vo ; Hong, Soon-Ho ;
KSBB Journal, volume 24, issue 4, 2009, Pages 393~396
New protein secretion system was developed using Type III Secretion System of Salmonella. N-terminal region of SlrP and SptP effector proteins were fused with TliA and EstA-P lipases by overlapping PCR. Lipase activity of Salmonella with SptP-TliA fusion system increased by 2.6 fold compare with wild type Salmonella strain. This result showed that lipase secretion via the T3SS would be a useful protein secretion machinery.
The Melanin Inhibition, Anti-aging and Anti-inflammation Effects of Portulaca oleracea Extracts on Cells
Zhang, Rui ; Lee, Hyun-Jin ; Yoon, Yeong-Min ; Kim, Su-Mi ; Kim, Hyun-Sook ; Li, Shun Hua ; An, Sung-Kwan ;
KSBB Journal, volume 24, issue 4, 2009, Pages 397~402
The Portulaca oleracea (P. oleracea) is a popular herbal medicine in East Asia that was known to possess detoxification, antifebrile and antifungal effects. In the present study, we examined the biological activities of ethanol extracts of P. oleracea under various conditions with NIH3T3, B16F10, and MCF-7 cell line model systems. Extracts of P. oleracea (0.5 mg/ml) showed inhibition of expression of tyrosinase, but does not suppress either of TYRP-1 or DCT expression on B16F10 cells. Extracts of P. oleracea (2 mg/ml) showed anti-inflammatory effects on TNF-
-Luc cells and increase of the synthesis of collagen on NIH3T3 (wild type) cells. These results suggest that extracts of P. oleracea could be used as a functional biomaterial in developing a skin whitening agent and having the anti-inflammatory, anti-wrinkle, and anti-aging activities.
H2AX Directly Interacts with BRCA1 and BARD1 via its NLS and BRCT Domain Respectively in vitro
Bae, Seung-Hee ; Lee, Sun-Mi ; Kim, Su-Mi ; Choe, Tae-Boo ; Kim, Cha-Soon ; Seong, Ki-Moon ; Jin, Young-Woo ; An, Sung-Kwan ;
KSBB Journal, volume 24, issue 4, 2009, Pages 403~409
H2AX, a crucial component of chromatin, is implicated in DNA repair, cell cycle check point and tumor suppression. The aim of this study was to identify direct binding partners of H2AX to regulate cellular responses to above mechanisms. Literature reviews and bioinformatical tools were attempted intensively to find binding partners of H2AX, which resulted in identifying two potential proteins, breast cancer-1 (BRCA1) and BRCA1-associated RING domain 1 (BARD1). Although it has been reported in vivo that BRCA1 co-localizes with H2AX at the site of DNA damage, their biochemical mechanism for H2AX were however only known that the complex monoubiquitinates histone monomers, including unphosphorylated H2AX in vitro. Therefore, it is important to know whether the complex directly interacts with H2AX, and also which regions of these are specifically mediated for the interaction. Using in vitro GST pull-down assay, we present here that BRCA1 and BARD1 directly bind to H2AX. Moreover, through combinational approaches of domain analysis, fragment clonings and in vitro binding assay, we revealed molecular details of the BRCA1-H2AX and BARD1-H2AX complex. These data provide the potential evidence that each of the BRCA1 nuclear localization signal (NLS) and BARD1 BRCA1 C-terminal (BRCT) repeat domain is the novel mediator of H2AX recognition.
SNPchaser : A Web-based Program for Detecting SNPs Substitution and Heterozygosity Existence
Jang, Jin-Woo ; Lee, Hyun-Chul ; Lee, Myung-Hoon ; Choi, Yeon-Shik ; Choo, Dong-Won ; Park, Kie-Jung ; Lee, Dae-Sang ;
KSBB Journal, volume 24, issue 4, 2009, Pages 410~414
Single-nucleotide polymorphisms (SNPs) are the DNA sequences difference among the same species in the level of nucleic acids and are widely applied in clinical fields such as personalized medicine. The routine and labor-intensive methods to determine SNPs are performing the sequence homology search by using BLAST and navigating the trace of chromatogram files generated by high-throughput DNA sequencing machine by using Chromas program. In this paper, we developed SNPchaser, a web-based program for detecting SNPs substitution and heterozygosity existence, to improve the labor-intensive method in determining SNPs. SNPchaser performed sequence alignment and visualized the suspected region of SNPs by using user's reference sequence, AB1 files, and positional information of SNPs. It simultaneously provided the results of sequences alignment and chromatogram of relevant area of SNPs to user. In addition, SNPchaser can easily determine existence of heterozygosity in SNPs area. SNPchaser is freely accessible via the web site http://www.bioinformatics.ac.kr/SNPchaser and the source codes are available for academic research purpose.