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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 24, Issue 6 - Dec 2009
Volume 24, Issue 5 - Oct 2009
Volume 24, Issue 4 - Aug 2009
Volume 24, Issue 3 - Jun 2009
Volume 24, Issue 2 - Apr 2009
Volume 24, Issue 1 - Feb 2009
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Evaluation of Biological and Physico-chemical Detoxification Methods for the Removal of Inhibitors in Lignocellulose Hydrolysate
Cho, Dae-Haeng ; Kim, Yong-Hwan ;
KSBB Journal, volume 24, issue 5, 2009, Pages 415~419
In this study, the detoxification methods were evaluated for the removal of fermentation inhibitors from synthetic solution containing the composition similar to the lignocellulosic hydrolysate. The enzyme peroxidase and laccase were used as a biological treatment method. The physico-chemical methods such as adsorption and ion exchange were applied by using activated charcoal and ion exchange resins. The enzyme peroxidase showed a excellent removal of phenolic compounds. The 5-HMF and furfural were completely removed by activated charcoal. The anion exchange resin showed a good result for detoxification of acetic acid. The activated charcoal and ion exchange resins lead to a loss of sugars more or less. The choice of detoxification method must be made after considering the composition and inhibitors in hydrolysates.
Simple Measurement of Livestock Foul Smells by Gas Detector Tube
Jung, Jae-Chil ; Ahn, Min-Ho ; Jeong, Jong-Won ; Choi, Hee-Seon ; Kim, Tai-Jin ;
KSBB Journal, volume 24, issue 5, 2009, Pages 420~424
The present study was aimed for the fetor in the livestock facilities. Simple detection systems for ammonia and hydrogen sulfide were examined to see the mechanism making the discoloration length of malodorous substance concentration. Detector tube for ammonia showed a good linearity of 99.6% while hydrogen sulfide detector tube gave 99.7% linearity with reproductivity of 95%.
Effects of Hepatocyte Growth Factor on the PSA Signaling Pathway of U-251-MG Cells
Kim, Hwan-Gyu ;
KSBB Journal, volume 24, issue 5, 2009, Pages 425~431
Hepatocyte growth factor (HGF) and its receptor play an important role in the formation and progression of glioma. In this study, I investigated the ability of HGF to recover of the PSA siRNA-suppressed cell proliferation, migration and invasion in U-251-MG cells. PSA siRNA-transfected U-251-MG cells showed the reduction of the proliferation, migration and invasion with compared to control. Treatment of HGF on the PSA siRNA-transfected U-251-MG cells recovered the ability of proliferation, migration and invasion. These data suggest that PSA and HGF may use unique and parallel signaling cascade leading to the proliferative, migrative and invasive phenotype of U-251-MG cells. I also showed that PSA cooperated with HGF to a migrative and invasive phenotype via the increased secretion of matrix metalloproteinase-2 (MMP-2) and MMP-9.
Highly Sensitive Detection of Pathogenic Bacteria Using PDMS Micro Chip Containing Glass Bead
Won, Ji-Yeong ; Min, Jun-Hong ;
KSBB Journal, volume 24, issue 5, 2009, Pages 432~438
Here, we demonstrated simple nucleic acid, RNA, concentration method using polymer micro chip containing glass bead (
). Polymer micro chip was fabricated by PDMS (
in the height) including pillar structure (
, gap size
) for blocking micro bead. RNA could be adsorbed on micro glass bead at low pH by hydrogen bonding whereas RNA was released at high pH by electrostatic force between silica surface and RNA. Amount of glass beads and flow rate were optimized in aspects of adsorption and desorption of RNA. Adsorption and desorption rate was measured with real time PCR. This concentrated RNA was applied to amplification micro chip in which NASBA (Nucleic Acid Sequence Based Amplification) was performed. As a result, E.coli O157 : H7 in the concentration of 10 c.f.u./10 mL was successfully detected by these serial processes (concentration and amplification) with polymer micro chips. It implies this simple concentration method using polymer micro chip can be directly applied to ultra sensitive method to measure viable bacteria and virus in clinical samples as well as environmental samples.
Effect of Biomass-derived Inhibitors on Ethanol Production
Lee, Myung-Gu ; Cho, Dae-Haeng ; Kim, Yong-Hwan ; Lee, Jin-Won ; Lee, Jong-Ho ; Kim, Seung-Wook ; Cho, Jae-Hoon ; Lee, Do-Hoon ; Kim, Sang-Yong ; Park, Chul-Hwan ;
KSBB Journal, volume 24, issue 5, 2009, Pages 439~445
The process for ethanol production requires lignocellulosic biomass to be hydrolyzed to generate monomeric sugars for the fermentation. During hydrolysis step, a monomeric sugars and a broad range of inhibitory compounds (furan derivatives, weak acids, phenolics) are formed and released. In this study, we investigated the effects of inhibitory compounds on the fermentative performance of Saccharomyces cerevisiae K35 and Pichia stipitis KCCM 12009 in ethanol production, two yeast strains were fermented in the synthetic medium including six inhibitory compounds such as 5-hydroxymethylfurfura (5-HMF), furfural, acetic acid, syringaldehyde, vanillic acid and syringic acid. Ethanol of over 40 g/L was produced by two yeast strains in the absence of inhibitory compounds, respectively. Most inhibitory compounds except acetic acid had a little effect on the ethanol production, but acetic acid showed high inhibition effect on the cell growth and ethanol production.
Evaluation of Extractants for Bio-butanol Extraction Fermentation Using Organic Solvents and Ionic Liquids
Cho, Min-Ok ; Lee, Sun-Mi ; Sang, Byoung-In ; Um, Young-Soon ;
KSBB Journal, volume 24, issue 5, 2009, Pages 446~452
Oleyl alcohol, butyl butyrate, and two different ionic liquids were evaluated for the extraction of butanol from culture broth without toxic effect to cells. The tested solvents showed more than 50% extraction efficiency, and oleyl alcohol was chosen as the best extractant for butanol among the used extractants with a partition coefficient of 2.89. When oleyl alcohol was used as an extractant, more than 80% of butanol was extracted in the wide range of butanol concentrations (1-20 g/L) and pH values (pH 4-5.5). In extractive fermentation using oleyl alcohol only, there was 11% more butanol production and glucose consumption when compared to that without extractive fermentation, implicating a reduced inhibitory effect of butanol due to butanol removal to the oleyl alcohol phase. In addition, oleyl alcohol did not inhibit cell growth, while a mixture of oleyl alcohol and butyl butyrate with the volume ratio of 9:1~7:3 inhibited either butanol production or cell growth significantly due to the toxicity of butyl butyrate to cells. In conclusion, oleyl alcohol can be used as an efficient and non-toxic solvent for extractive fermentation for butanol production.
Biodegradation of Diesel in Sea Water by Rhodococcus fascians Isolated from a Petroleum-contaminated Site
Koo, Ja-Ryong ; Moon, Jun-Hyung ; Yun, Hyun-Shik ;
KSBB Journal, volume 24, issue 5, 2009, Pages 453~457
Contamination of marine environment with hazardous and toxic chemicals is more common these days. Bioremediation is the application of microorganism or microbial processes to degrade environmental contaminant. Because of low water solubility and volatility of diesel, bioremediation is more efficient than physical and chemical methods. The objective of this study is biodegradation of diesel in sea water by using Rhodococcus fascians which is isolated petroleum-contaminated soil. R. fascians was cultured on sea water containing diesel to determine the diesel degradability. Changes in biodegradability of diesel with various inoculum sizes, diesel concentrations, initial pH, and culture temperature were analyzed by TPH analysis using gas chromatography. The inoculum size 2% was effective for biodegrdation of diesel in sea water by R. fascians. When diesel concentration was 5%, the growth of cell was inhibited by the toxicity of diesel. The optimal temperature and initial pH for degradation of diesel in sea water were
and pH 8.
Preparation and Characterization of Sulfated Fibroin
Jeong, Dae-Cheol ; Lee, Shin-Young ; Hur, Won ;
KSBB Journal, volume 24, issue 5, 2009, Pages 458~462
Silk fibroin is a structural protein from Bombyx mori and can be sulfated to provide biofunctional polypeptides showing antiviral and anticoagulating activities. However, the sulfated fibroins have not been characterized enough to present their compositions and structures. In this report, sulfation reaction was investigated by varying the reaction temperature and sulfuric acid concentration and analysis of the resulting peptides were carried out. The degree of sulfation was proportion to sulfuric acid concentration but the maximum product yield was obtained at
and 5% of sulfuric acid concentration. FT-IR spectrum, UV absorption and NMR spectrum support that o-sulfate ester was formed at the hydroxyl group of serine and tyrosine residues. Size exclusion chromatography identified that sulfated fibroin was composed of a highly hydrolyzed polypeptide mixtures and peptides of relatively higher molecular weight.
UVB Protective Effect of Yeast Originated Selenium Peptide on Fibroblast
Lee, Hyang-Bok ; Lee, Jung-Ok ; Nguyen, Dung H. ; Yoon, Sun-A ; Um, Ji-Min ; Lee, Yu-Ri ; Moon, Hyung-In ; Chung, Jin-Ho ; Kim, Eun-Ki ;
KSBB Journal, volume 24, issue 5, 2009, Pages 463~468
Selenium-containing peptide (Selenium peptide) was produced by autolysis of Saccharomyces cerevisiae which was cultured in inorganic selenium-supplemented medium. Selenium peptide showed antioxidant activity and protective effects on UVB irradiated human fibroblast. Minimal toxicity of selenium peptide was observed whereas selenium nitrate exhibited cell toxicity as low as
. Selenium peptide also increased human fibroblast growth, procollagen type I and also decreased MMP-1 (matrix metalloprotease-1). This result showed the potential of selenium peptide as a nontoxic antioxidant.
Antioxidant Activity of the Salt Marsh Plant Corydalis heterocarpa
Kim, You-Ah ; Lee, Jung-Im ; Lee, Jin-Hyeok ; Kong, Chang-Suk ; Nam, Taek-Jeong ; Seo, Young-Wan ;
KSBB Journal, volume 24, issue 5, 2009, Pages 469~476
The antioxidant activities of two crude extracts (
and MeOH) and their solvent fractions (n-hexane, 85% aq. MeOH, n-BuOH, and
fractions) from Corydalis heterocarpa were determined by evaluating authentic
generated from SIN-1 (3-morpholinsydnonimine) in vitro as well as by measuring the degree of occurrence of intracellular reactive oxygen species (ROS) and nitric oxide (NO). Scavenging activities of solvent fractions on authentic
increased in the order of n-BuOH > 85% aq. MeOH >
> n-hexane fractions, while those on
generated from SIN-1 increased in the order of n-BuOH > 85% aq. MeOH >
> n-hexane fractions. In addition, all solvent fractions effectively inhibited the intracellular ROS and NO levels. The n-BuOH fraction especially exhibited the strongest ROS scavenging effect. Further purification of n-BuOH fraction led to the isolation of cnidimoside A, which presented the potent ROS scavenging effect at
. From these results, extracts of C. heterocarpa and its component, cnidimoside A, were predicted to be potentially useful as ingredients for protecting against oxidation.
Extraction and Isolation of Antioxidant Fraction from Waste of Grape Products for Cosmetic Application
Kim, Eui-Jin ; Lee, Tae-Ho ; Shin, Hyun-Jae ;
KSBB Journal, volume 24, issue 5, 2009, Pages 477~482
Anthocyanin and origomeric proanthocyanidin (OPC) fractions showing antioxidizing activity are present in grape extracts. Grape extracts are widely used in cosmetic applications as functional ingredients. The aim of our study is to isolate the antioxidant fraction from waste of grape products. The extraction was done using soxhlet apparatus. Next, the extraction was subjected for identification of antioxidants by using HPLC. Antioxidant assay was performed by using 2,2-diphenyl-picryl-hydrazyl (DPPH) and superoxide dismutase (SOD) method. Antioxidizing activity was found to be higher in grape seed extract when compared to grape skin extract. Using the extract, a novel formula with multi lamella emulsion structure has been developed and its safety and stability were confirmed by standard protocols.
Production of Bio-ethanol from Brown algae by Enzymic Hydrolysis
Lee, Sung-Mok ; Choi, In-Soon ; Kim, Sung-Koo ; Lee, Jae-Hwa ;
KSBB Journal, volume 24, issue 5, 2009, Pages 483~488
The Brown-algae polysaccharide consisting of alginate and laminaran is usable as high bio-ethanol production if hydrolyzed to monomer unit. The objective of this study is to produce bio-ethanol from brown-algae using enzymatic saccharification. Bio-ethanol was produced by Saccharomyces cerevisiae KCCM 1129 and Pachysolen tannophilus KCTC 7937 strains. The substrate used Laminaria japonica, Sargassum fulvellum and Hizikia fusiformis. We isolated a new alginate lyase and laminaran lyase producing microorganism for hydrolysis of brown-algae from southern sea of Gijang. The reducing sugar was obtained 1.90 g/L from Laminarin japonica 20 g/L that used enzyme from Bacterium antarctica. In pretreatment of the most suitable brown-algae for ethanol production, ethanol concentration of 0.93 g/L and yield of 4.65% were obtained in condition of Laminaria japonica in medium.
Two-step High Temperature Pretreatment Process for Bioethanol Production from Rape Stems
Han, Jae-Gun ; Oh, Sung-Ho ; Jeong, Myoung-Hoon ; Kim, Seung-Seop ; Seo, Hyeon-Beom ; Jeong, Kyung-Hwan ; Jang, Young-Seok ; Kim, Il-Cheol ; Lee, Hyeon-Yong ;
KSBB Journal, volume 24, issue 5, 2009, Pages 489~494
Two-step pretreatment process was investigated to efficiently hydrolyzed rape stems for obtaining fermentable sugars. The process was consisted of two consecutive steps as
and 15 MPa and
and 24 MPa with the flow rate of 5 mL/min. Under this condition, 5.5 (g/L) of glucose and 25.6 (g/L) of xylose were obtained from rape stems, showing 18% of glucose yield based on 25% cellulose in the rape stems. It was also found that this process could generate less amounts of toxic residues, such as HMF (Hydroxy- Methyl-Furfural) and other fulfural components during hydrolysis process. It could reaction maintain relatively high ethanol production yield as 90% of theoretical conversion yield from glucose. Therefore, this pretreatment process could be applied to hydrolyze other cellulosic and marine resources such as woods, stem and algae for bioethanol production.