Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal Basic Information
Journal DOI :
Korean Society for Biotechnology and Bioengineering
Editor in Chief :
Volume & Issues
Volume 24, Issue 6 - Dec 2009
Volume 24, Issue 5 - Oct 2009
Volume 24, Issue 4 - Aug 2009
Volume 24, Issue 3 - Jun 2009
Volume 24, Issue 2 - Apr 2009
Volume 24, Issue 1 - Feb 2009
Selecting the target year
Present Status and Prospects of Marine Chemical Bioindustries
Lee, Sun-Bok ; Cho, Sun-Ja ; Lee, Shin-Youb ; Paek, Kwang-Hyun ; Kim, Jeong-A ; Chang, Jin-Hwa ;
KSBB Journal, volume 24, issue 6, 2009, Pages 495~507
As we move into the 21st century, the importance of marine resources is certain to increase due to the accelerated exhaustion of land resources. For the sustainable development of the world, therefore, we need to develop marine chemical bioindustries which enable us to produce industrial chemicals, advanced materials, fuels, and minerals from marine resources such as seaweeds and seawater. In this review, we selected five marine chemical bioindustries which include 1) seaweed polysaccharide industry, 2) marine advanced materials industry, 3) marine biofuel industry, 4) marine sea salt industry, and 5) deep-sea water industry, and discussed the current status and future prospects of each industry sector. It has been assessed that the future of marine chemical bioindustry looks very promising although there are many needs for more intensive research investments on marine bioprocess development through close cooperation between marine biologists and biochemical engineers.
Isolation and Characterization of Catalase-producing Bacteria from Soil
Han, Kyung-Ah ; Rhee, Jong-Il ;
KSBB Journal, volume 24, issue 6, 2009, Pages 508~514
This study investigated the production of catalase from Bul-kyo soil bacteria through fermentation process. Isolation and selection of bacteria was performed through chemical and physiological analysis. Catalases were produced from bacteria which belong to 3 different species (Bacillaceae bacterium BKBChE-1, Bacillus sp. BKBChE-2, Bacillus flexus BKBChE-3) confirmed by using 16S rDNA sequence method. The catalases were found to be stable in the temperature range of
for BKBChE-1, BKBChE-2 and BKBChE-3 and also in the pH range of 9.0-12.0 for BKBChE-1 and BKBChE-3. Long-term stability of the catalases was about 20 days at
. However, BKBChE-2 has kept its activity over 30 days at
Facile Cell Patterning Based on Selectively Patterned Polydimethylsiloxane (PDMS) and Polyelectrolyte Surface
Jeong, Heon-Ho ; Song, Hwan-Moon ; Hwang, Ye-Jin ; Hwang, Taek-Sung ; Lee, Chang-Soo ;
KSBB Journal, volume 24, issue 6, 2009, Pages 515~520
This study presented facile method of cell patterning using fabricated PDMS patterns on polyelectrolyte coated surface. This basic principle is the fabrication of functional surface presenting two orthogonal surfaces such as cell adhesive and repellent properties. Cell adhesive surface was firstly fabricated with simple coating of polyelectrolyte multilayer. And then, the desired patterns of PDMS for the prevention of nonspecific binding of cells were transferred onto the previously formed thin film of polyelectrolyte multilayer. Thus, we could prepare novel functional surface simultaneously containing PDMS and polyelectrolyte region. As expected, the PDMS regions showed effective prevention of nonspecific binding of cell and the other region, exposed polyelectrolyte area, provided cell adhesive environment. The height of formed PDMS structure was about 100 nm. Based on this method, cell patterning can be successfully obtained with various pattern shapes and sizes. Therefore, we expect that this simple method will be useful platform technology for the development of cell chip, cell based assay system, and biochip.
Comparative Studies on the Fatty Acid Compositions of the Korean Salt Marsh Plants in the West Sea
Kim, You-Ah ; Um, Young-Ran ; Lee, Jung-Im ; Kim, Hae-Jin ; Lim, Sun-Young ; Nam, Taek-Jeong ; Seo, Young-Wan ;
KSBB Journal, volume 24, issue 6, 2009, Pages 521~526
Fatty acid is an important component of many biological processes. However, an imbalance in diet-especially, a n-3 polyunsaturated fatty acids (PUFAs) deficiency-causes several diseases such as diabetes, cardiovascular disease and cancer. In this study, we analyzed the fatty acid contents and compositions of 26 species of Korean salt marsh plants and found high fatty acid contents from S. herbacea (
/mg-dry wt.), S. komarvii (
/mg-dry wt.), C. heterocarpa (
/mg-dry wt.), A. capillaris (
/mg-dry wt.), and L. tetragonum (
/mg-dry wt.). In the case of saturated fatty acids (SFAs) composition, palmitic acid is richest in most salt marsh plants. On the other hand, oleic acid and linoleic acid are major components of monounsaturated fatty acid and n-6 PUFA, respectively. In addition, n-3 PUFAs such as LNA (linolenic acid), EPA (eicosapentaenoic acid), and DHA (docosahexaenoic acid) known as the main fatty acid components of fish oils and seaweeds, were also found in S. herbacea, S. komarvii, T. tetragonoides, A. capillaris and G. littoralis.
Lignin Removal from Barley Straw by Ethanosolv Pretreatment
Kim, Young-Ran ; Yu, An-Na ; Chung, Bong-Woo ; Han, Min-Hee ; Choi, Gi-Wook ;
KSBB Journal, volume 24, issue 6, 2009, Pages 527~532
Lignocellulose represents a key sustainable source of biomass for transformation into biofuels and bio-based products. Unfortunately, lignocellulosic biomass is highly recalcitrant to biotransformation, both microbial and enzymatic, which limits its use and prevents. As a result, effective pretreatment strategies are necessary. The vast majority of pretreatment strategies have focused on achieving a reduction of lignin content. In this work, an ethanosolv pretreatment has been evaluated for extracting lignin from barley straw. 75% ethanol was used as a pretreatment solvent to extract lignin from barley straw. The influence on delignification of three independent variables are temperature, time, catalyst (1 M
) dose. The best pretreatment condition observed was
, 120 min, 0.2%
and delignification was 38%. A combined roasting and ethanosolv, 2-step pretreatment, was developed in order to improve the delignification. Roasting didn't increase the delignification but reduced the pretreatment time. X-ray diffraction results indicated that these physical changes enhance the enzymatic digestibility in the ethanosolv treated barley straw. The cellulose in the pretreated barley straw becomes more crystalline without undergoing ethanosolv.
Preparation of Solid Dispersions of a Poorly Water-soluble Drug Using Supercritical Fluid
Kim, Seok-Yun ; Lee, Jung-Min ; Jung, In-Il ; Lim, Gio-Bin ; Ryu, Jong-Hoon ;
KSBB Journal, volume 24, issue 6, 2009, Pages 533~540
In this work, 5'-nitroindirubinoxime (5'-NIO) has been prepared as solid dispersions using a supercritical aerosol solvent extraction system (ASES) process in order to enhance its water solubility and dissolution rate. Solid dispersions of 5'-NIO and poly(vinyl pyrrolidone) (PVP) were prepared in various weight percent ratios. Three-component solid dispersions consisting of 5'-NIO, PVP, and poloxamer 188 (P188) were also prepared to study the influence of P188 level on their morphology, crystallinity, and dissolution behavior. All samples were prepared at
and 180 bar using supercritical carbon dioxide. The particle morphology and size of the two-component solid dispersions were found to be nearly spherical and much smaller (100-200 nm) compared with the original 5'-NIO. The morphology of three-component solid dispersions became more agglomerated as the level of P188 increased. The crystallinity of the original 5'-NIO was not observed in the solid dispersions prepared by the ASES process. Faster dissolution rates were observed for the three-componet solid dispersions because the arrangement of ethylene oxide and propylene oxide blocks of the poloxamer 188 enabled the formation of micelles in an aqueous phase.
Anti-Cell Adhesion Effect of Animal Cell with Regional Special Natural Products of Anthrisci radix, Psoraleae semen, Siegesbeckiae herba and Corni fructus
Shin, Jin-Hyuk ; Cha, Gu-Yong ; Kim, Hui-Jin ; Hwang, Jae-Ho ; Han, Kyeong-Ho ; Seo, Hyo-Jin ; Kim, Min-Yong ; Oh, Suk-Jung ; Kim, Jong-Deog ;
KSBB Journal, volume 24, issue 6, 2009, Pages 541~548
For investigation of anti-angiogenesis mechanism of Anthrisci radix, Psoraleae semen, Siegesbeckiae herba and Corni fructus, anti-cell adhesion experiment was performed. The adhesion of U937 cells to IL-
-stimulated HUVECs was completely suppressed by 276% at 0.2 mg/L of Anthrisci radix, 220% at 5 mg/L of Psoraleae semen, 158% at 10 mg/L of Siegesbeckiae herba and 132% at 20 mg/L of Corni fructus, respectively. And the adhesion of PMA-chemical stimulated U937 cells to HUVECs, it was inhibited 139% at 0.2 mg/L of Anthrisci radix, 442% at 5 mg/L of Psoraleae semen, 720% at 10 mg/L of Siegesbeckiae herba and 664% at 20 mg/L of Corni fructus. Also, the adhesion of chemical stimulated U937 cells to IL-
/chemical stimullated HUVECs, it was inhibited by 286% at 0.2 mg/L of Anthrisci radix, 146% at 5 mg/L of Psoraleae semen, 436% at 10 mg/L of Siegesbeckiae herba and 297% at 20 mg/L of Corni fructus, respectively. It would be a useful substance for anti-cell adhesion based on anti-angiogenesis for anti-obesity and anti-cancer.
Exmination of Anti-Obesity Effect of Regional Special Natural Products of Anthrisci radix, Psoraleae semen, Siegesbeckiae herba and Corni fructus
Shin, Jin-Hyuk ; Cha, Gu-Yong ; Kim, Hui-Jin ; Hwang, Jae-Ho ; Han, Kyeong-Ho ; Seo, Hyo-Jin ; Shin, Tai-Sun ; Oh, Suk-Jung ; Kim, Jong-Deog ;
KSBB Journal, volume 24, issue 6, 2009, Pages 549~555
4 kinds of Regional Special Natural Products (RSNPs), such as Anthrisci radix, Psoraleae semen, Siegesbeckiae herba and Corni fructus were examined to verify for anti-obesity effect.
(peroxisome proliferator-activated receptor
) from 3T3-L1 cell concerning adipocyte differentiation was suppressed by different concentraton of 4 RSNPs with western blot, when treated RSNPs' extract and MDI (IBMX, Dexamethasone, Insulin) at the same time. Also, SREBP-1 (Sterol regulatory element binding protein) controlling lipogenesis and
expression levels were reduced by these 4 RSNPs' extract, when these chemicals after differentiation of 3T3-L1 cell. And lipid droplets were reduced by 7.5%, 14.4%, 18.3% and 30% at different concentration of Anthrisci radix from Oil Red O staining. Also, it was reduced by 2%, 4.9%, 9.3% and 38% at different concentration of Psoraleae semen. For Siegesbeckiae herba, it was inhibited by 1.4%, 6.4%, 16.4% and 30.1%, respectively. And Corni fructus was also showed by 0.9%, 6.3%, 13.7% and 33% at same concentration of Siegesbeckiae herba. These 4 kinds of RSNPs were expected for a useful material for anti-obesity materials.
Biological Treatment of TNT-containing Wastewater (pink water) by Stenotrophomonas maltophilia OK-5, and RT-PCR Quantification of the Nitroreductase (pnrB) Gene
Cho, Su-Hee ; Cho, Yun-Seok ; Oh, Kye-Heon ;
KSBB Journal, volume 24, issue 6, 2009, Pages 556~562
The biological treatment of TNT-containing wastewater, known commonly as pink water, was investigated using a stirred tank reactor with Stenotrophomonas maltophilia OK-5 bacterial culture. S. maltophilia OK-5 exhibited effective degradation of TNT contained in pink water, completely degrading TNT (100 mg/L) within 6 days of incubation. The dark-red brown color derived from Hydride-Meisenheimer complex became more pronounced during the incubation period, which was determined quantitatively. High-pressure liquid chromatography was used to measure residual TNT, which also resolved the metabolic intermediates (i.e., 2,4-dinitrotoluene, 2,6-dinitrotoluene and 2,4-dinitro-6-hydroxytoluene). Gas chromatography-mass spectrometry was used to verify these intermediates. Quantification of the nitroreductase (pnrB) gene isolated from S. maltophilia OK-5 growing in pink water was performed with real-time PCR. The amount of pnrB gene copies increased to
-fold after 5 days of incubation time.
Preparation of Semi-solid Fibroin Gel and its Flow Property
Hur, Won ; Lee, Shin-Young ;
KSBB Journal, volume 24, issue 6, 2009, Pages 563~569
Fibroin is an insoluble structural protein from Bombyx mori. It can be solubilized by dissolving in a hot
solution and subsequent dialysis. The aqueous solution is unstable and a transition from aqueous fibroin molecules rich in random coil is undergo to one rich in
-sheet content, resulting in hydrogelation. However, fibroin gel is so fragile and plastic that its mechanical property should be reformed for various applications. In this report, a semi-solid form of fibroin gel was prepared using glycerol and ethanol and was investigated to analyze their flow properties. A fibroin gel with 80% glycerol showed pseudoplastic and thixotropic properties. The square root of its yield stress varied linearly with fibroin concentration and it extrapolated to zero shear stress at 0.2% fibroin. A fibroin gel with 40% ethanol was shown to be highly thixotropic but its shear-thinning behavior was only observed above a certain level of shear rate. Its pseudoplasticity was restored by a high rate of shear stress.
Physiological Activity of Astaxanthin and its Inclusion Complex with Cyclodextrin
Kim, So-Young ; Cho, Eun-Ah ; Yoo, Gui-Jae ; Yoo, Ji-Min ; Son, Seok-Min ; In, Man-Jin ; Kim, Dong-Chung ; Chae, Hee-Jeong ;
KSBB Journal, volume 24, issue 6, 2009, Pages 570~578
In vitro biological activities such as antioxidant, whitening, anti-hangover and anticancer activities were evaluated. The antioxidant activities of astaxanthin and H. pluvialis extract were significantly higher than that of
-tocopherol when the antioxidant activities were determined as xanthine oxidase inhibition, hydroxyl radical scavenging and DPPH radical scavenging. The whitening effect of H. pluvialis extract was about two times as kojic acid. H. pluvialis extract indicated an anticancer activity on a cervical cancer cell line. Astaxanthin showed anti-hangover effect of 1.5 times as jiguja extract. The anti-hangover effect of the inclusion complex (As-
-CD) was about 1.2 times of jiguja extract. And, the inclusion complex of Haematococcus pluvialis (H.p.-
-CD) showed almost the same whitening effect as kojic acid.
Anti-oxidant and Whitening Effects of Cell Lytic Enzyme-treated Lotus Leaf Extract
Choi, Sun-Ju ; Kim, So-Young ; Lee, Sung-Chul ; Lee, Jin-Man ; Lee, In-Suk ; Jung, Moon-Yung ; Yang, Sam-Man ; Chae, Hee-Jeong ;
KSBB Journal, volume 24, issue 6, 2009, Pages 579~583
The effects of cell lytic enzyme treatment on total phenolic content, antioxidant and antityrosinase activities of lotus leaf were investigated. The dried lotus leaves were hydroyzed by cell lytic enzymes such as Promozyme, Ceremix, Pectinex, Ultraflo, Celluclast, Pentopan, Tunicase, Viscozyme at their optimum pHs (pH 5-8) at
for 4 hrs. Depending on the enzymes used, total phenolic compounds content was measured as
/mL, and antioxidant activities and whitening activities were increased by 5~10% and 20%, respectively Among the tested hydrolytic enzymes, Promozyme (pullulanase) was selected as the most suitable enzyme for the extraction of total polyphenol from lotus leaf. The optimal dosage of Promozyme were found to be 1-2% (w/w). By Promozyme treatment, total phenolic compounds content of the lotus extract significantly increased compared to the extraction without enzyme treatment.
Composition and Structural Characteristics of Polysaccharide from Hot Water Extraction of Auricularia auricula
Kim, Hyeon-Min ; Hur, Won ; Lee, Shin-Young ;
KSBB Journal, volume 24, issue 6, 2009, Pages 584~590
A hot-water extract from fruit body of Auricularia auricula was purified by ethanol precipitation and subsequent dialysis. The polysaccharide showed a typical IR spectrum similar to
-glucan and was composed of glucose and mannose in a molar ratio of 16.8:83.2 indicating that it is a glucomannan. A glucomannan-like polysaccharide was also identified from Tremella fuciformis. Both of the polysaccahrides showed the presence of acetyl groups and presented colorimetric responses for
-1,4-glucomannan indicating the acetylated
-glucomannan similar to the typical polysaccharide found in Aloes.
Functional Expression of Anti-BNP scFv in E. coli Cytoplasm for the Detection of B-type Natriuretic Peptide
Maeng, Bo-Hee ; Nam, Dong-Hyun ; Kim, Yong-Hwan ;
KSBB Journal, volume 24, issue 6, 2009, Pages 591~597
B-type natriuretic peptide is a neurohormone secreted in the cardiac ventricles. BNP levels are elevated in patients with ventricular dysfunction. Therefore, the concentration of BNP is important factor to reflect diagnosis and prognosis for cardiovascular disease. In this respect, anti-BNP scFv is an urgent requirement for early diagnosis in the field of biosensor. Herein, the genetic codes of anti-BNP scFv were chemically synthesized and cloned into both pET22b (+) and pColdⅣ vector, respectively. The recombinant scFv was successfully expressed as a functional form in cytoplasm of E. coli and detected through Western blot and ELISA. The highest level of functional expression of anti-BNP scFv was achieved using pET22b (+) vector at
by addition of 0.1 mM IPTG. Additionally, being exposed to both BNP and ANP, anti-BNP scFv specifically captured only BNP. Therefore, anti-BNP scFv expressed in this study will be applied to measure the concentration of BNP as a diagnostic recognition molecule.
Transformation of Cell Wall-weakened Perilla Seedlings Using Phenolic Compound-treated Agrobacterium Cells and Recombinant Protein Expression
Chung, Il-Kyung ; Shin, Dong-Il ; Park, Hee-Sung ;
KSBB Journal, volume 24, issue 6, 2009, Pages 598~601
Perilla [Perilla frutescens (L.) Britt] seedlings are easy to grow and eaten as the health vegetable sprout. Two day old perilla seedlings since germination were given a mild wounding using cell wall lytic NaOH/SDS solution for infiltration with recombinant Agrobacterium cells treated with phenolic compounds. In the analysis of fluorometric GUS gene expression for the transformed perilla seedlings, GUS enzyme activity was the highest by the combined treatments of 50 mM acetosyringone and 0.5% NaOH solution containing 0.01% SDS implying a synergic effect. This result could be successfully applied for demonstrating hepatitis B virus antigen (HBsAg) protein expression.