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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 25, Issue 6 - Dec 2010
Volume 25, Issue 5 - Oct 2010
Volume 25, Issue 4 - Aug 2010
Volume 25, Issue 3 - Jun 2010
Volume 25, Issue 2 - Apr 2010
Volume 25, Issue 1 - Feb 2010
Selecting the target year
State-of-the-art of Life Cycle Assessment for Biodiesel Production from Plant Biomass
Seo, Bong-Kuk ; Song, Seung-Koo ;
KSBB Journal, volume 25, issue 1, 2010, Pages 1~10
Biodiesel is a type of biofuel obtained from bioresources and able to use in diesel vehicles as an alternative/additive to petro diesel. In recent biodiesel research, there are three main issues which include high quality biodiesel, low cost feed stock and a highly efficient biodiesel production process. The sustainable production and use of biodiesel are attracting much attention in the renewable energy field. In this paper, we review some of the literatures related to environmental and economic evaluation for biodiesel production and analysis the issues including life cycle assessment (LCA), global warming potential (GWP), energy consumption, biodiesel production cost, production technologies and feedstock.
Effect of Thrombin on the TNF-
Induced IL-6 Production in HUVECs
Bae, Jong-Sup ; Park, Moon-Ki ;
KSBB Journal, volume 25, issue 1, 2010, Pages 11~17
Here, we evaluated the effect of thrombin on the interleukin-6 production induced by tumor-necrosis-factor-
in endothelial cells. It is well known that tumor-necrosis-factor-
mediates inflammatory responses by activation of nuclear factor-kappa-B in endothelial cells. Here, we showed that lower concentration of thrombin decreased the production of interleukin-6 induced by tumor-necrosis-factor-
and this inhibitory effect of thrombin on interleukin-6 production was mediated by interacting with protease-activated-receptor-1. In addition, phosphoinositide-3-kinase was also involved the anti-inflammatory responses by lower concentration of thrombin in endothelial cells. These results suggested that lower concentration of thrombin mediated anti-inflammatory responses by interacting with protease-activated-receptor-1 on the cell membrane and phosphoinositide-3-kinase in the cell. These findings will provide the important evidence in the development of new medicine for the treatment of severe sepsis and inflammatory diseases and good clue for understanding unknown mechanisms by which thrombin showed the pro-inflammatory or anti-inflammatory activities in endothelial cells.
Whitening Effect of the Extracts from Juglans mandshurica
Lee, Gye-Won ; Lee, Ju-Yeon ; Cho, Young-Ho ;
KSBB Journal, volume 25, issue 1, 2010, Pages 18~24
Juglans mandshurica belongs to the family Juglandaceae is known to contain a wide range of pharmacological activities including anti-cancer, anti-inflammation, astringent, and anti-human immunodeficiency virus-type 1 (HIV-1). Melanogenesis refers to the biosynthesis of melanin pigment in melanocytes. In this study, to investigate the whitening activity of the extracts from Juglans mandshurica, we measured effects on a tyrosinase activity, a melanogenesis, and a tyrosinase synthesis in the B16/BL6 melanoma cells and an antioxidant activity. The extracts significantly scavenged a 1,1-diphenyl-2-picrylhydrazyl (DPPH) and a superoxide anion radicals in a dose-dependent manner with a
, respectively. Also, the tyrosinase activity and melanogenesis were significantly inhibited by the extracts. Furthermore, the synthesis of tyrosinase protein was significantly decreased by the extracts in enzyme-linked immunosorbent assay. Double blind study on the clinical efficacy of a cream containing 2% of the extracts showed that the extracts have a significant skin whitening effect. Therefore, this study demonstrates that the extracts from Juglans mandshurica may be useful as a potential agent for skin whitening.
A Comparison of Single Disinfection Process for Inactivation of E. coli
Kim, Dong-Seog ; Song, Seung-Koo ; Park, Young-Seek ;
KSBB Journal, volume 25, issue 1, 2010, Pages 25~32
This study was carried out to evaluate the performance of three kinds of single process (electrolysis, UV and ultrasonic process) for the purpose of disinfection of Escherichia coli in water. Among the five kinds of electrode material, disinfection performance of Ir electrode was higher than that of the other electrodes. The order of disinfection performance for E. coli in single process lies in: electrolysis > UV
ultrasonic process. Performance of the three single processes was increased with the increase of the electric power. Disinfection efficiency of the three processes was increased with the decrease of the pH. Disinfection of the UV process were decreased by the increase of NaCl dosage and air flow rate. However, ultrasonic process was not affected above two parameters. OH radical was not produced in UV and ultrasonic process. E. coli disinfection of the electrolysis process was well agreed with RNO degradation tendency, except pH.
E. coli Inactivation using Complex Disinfection Process
Kim, Dong-Seog ; Park, Young-Seek ;
KSBB Journal, volume 25, issue 1, 2010, Pages 33~40
Conventional disinfectants and disinfection method are expensive, hazardous and often require long periods of exposure. Recently, there is growing interest in complex disinfection process as a disinfection technique in medical instruments such as endoscope, hand piece bur to improve the disinfection efficiency and conveniency. The aim of this study was to evaluate the performance of a new complex process for the purpose of disinfection of Escherichia coli in water. Three single process (electrolysis, UV and ultrasonic process) was combined dual and triple disinfection process. The order of disinfection performance for E. coli in dual process lie in: Electrolysis + UV > Electrolysis + Ultrasonic > UV + Ultrasonic process. Disinfection efficiency of E. coli and degradation of N, N-Dimethyl-4-nitrosoaniline (RNO, indicating material of OH radical formation) of dual process was higher than that of the triple process (Electrolysis + UV + Ultrasonic process). In electrolysis + UV process, disinfection tendency was well agreed with RNO degradation tendency.
Dewatering Characteristics for Physico-chemical Properties with Sewage Sludge
Lee, Chang-Han ; Ahn, Kab-Hwan ; Song, Seung-Koo ;
KSBB Journal, volume 25, issue 1, 2010, Pages 41~46
In this study, we found that dewatering properties were related with physico-chemical properties such as sludge concentration, pH, and zeta potential with sewage sludge, Ionic contents in digested sludge were in the order of
(14.2 mg/g) >
(1.9 mg/g) >
(1.4 mg/g) >
(1.0 mg/g). Divalent ion more than monovalent have influenced on physico-chemical and dewatering properties. Floc size distribution in activated (AS) and digested sludge (DS) increased on lower pH range to be near isoelectric point and than specific resistance to filtration (SRF) decreased. A linear increase in SRF (
m/kg in AS and
m/kg in DS) was observed throughout low concentration range of 1230~9960 mg/L in AS and 2700~5400 mg/L in DS.
Morphological Changes by Submerged Culture Conditions for the Mycelial Optimal Growth of Cordyceps sinensis and Immunological Properties of Hot Water Extract of Mycelium
Suh, Hyung-Joo ; Choi, Jang-Won ;
KSBB Journal, volume 25, issue 1, 2010, Pages 47~54
The mycelial dispersed growth of Cordyceps sinensis was optimized in submerged batch culture at initial pH of 5.0, 150 rpm, and
. The morphological data showed much more dispersed growth of C. sinenesis at initial pH of 5.0. Also, projected area, main hyphal length and number of tips for the mycelial growth of initial pH 5.0 were higher than those of other initial pHs. The industrial medium for mycelial production of C. sinensis was determined to be molasses of 100 g and crushed brewery yeast of 10 g per liter as carbon and nitrogen sources, respectively. With these culture conditions, the maximum production of mycelia was approximately 30.0 g per liter by batch culture in 5-liter jar fermenter with no controlled pH. This result suggests that large-scale mycelia production of C. sinensis may be possible in submerged batch culture. The hot water extract of mycelia from C. sinensis was mainly composed of 83.0% carbohydrate, 11.8% protein, 1.9% lipid, and 2.4% ash and there were present glucose, mannose, galactose, and arabinose as molar ratio of 8.79 : 2.59 : 1.34 : 1.0 in the carbohydrate, respectively. In the experiment using spleen cell and macrophage, the extract showed potent mitogenic and immuno-stimulating activities and among various components, an important factor that contribute to the immunological activities was turned out to be carbohydrate moiety.
In Vitro Evaluation for Antioxidant Activities of Culture Broth of Tremella fuciformis
Ra, Kyung-Soo ; Choi, Jang-Won ;
KSBB Journal, volume 25, issue 1, 2010, Pages 55~61
To investigate antioxidant activity of the culture broth from submerged culture of Tremella fuciformis, we preferentially analyzed the chemical composition of culture broth, which was mainly composed of carbohydrate (296.39 mg/g) and protein (9.24 mg/g), respectively. Also, contents of polyphenols, flavonoids and flavonols were 16.63 mg/g, 9.19 mg/g and 83.74
, respectively. Next, we examined the scavenging abilities on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radical, the reducing power, and chelating ability on ferrous ions. All antioxidant activities of the culture broth were increased in proportion to its concentrations. The
values were in order as follows ABTS radical scavenging activity < OPPH radical scavenging activity < chelating power. Accordingly, these results suggest that pharmacological function of T. fuciformis might be due to, at least partially, their protective effects against oxidation and the culture broth of T. fuciformis was free radical inhibitors or scavengers at low concentration, involving possibly in termination of free radical reaction as primary antioxidants.
Phosphorus Removal by DPAOs (Denitrifying Phosphorus Accumulating Organisms) in Aerobic Condition
Jeong, No-Sung ; Park, Young-Seek ; Kim, Dong-Seog ;
KSBB Journal, volume 25, issue 1, 2010, Pages 62~66
This study was carried out to get phosphorus uptake rate in aerobic condition with nitrate and nitrite. Nitrate and nitrite inhibited phosphorus accumulating organisms' (PAOs') luxury uptake in aerobic condition. Nitrite awfully decreased the phosphorus uptake rate in aerobic condition. At the influent of 10 mg
-NL, the phosphorus uptake was decreased to 52% comparing that at no influent of nitrate. And at the influent of 10 mg
-NL, the phosphorus uptake was decreased to 28% comparing that at no influent of nitrite. At the influent of 20 mg
-NL, nitrite and nitrate were co-existed and the phosphorus uptake rate was decreased to 16% comparing that at no influent of nitrite and nitrate. Also, the denitrification was occurred by denitrifying glycogen accumulating organisms (DGAOs)/denitrifying phosphorus accumulating organisms (OPAOs) in spite of aerobic condition, and the phosphorus uptake rate was increased by the decrease of influent nitrate concentration at the aerobic condition. The inflection point in the phosphorus uptake rate was shown at the nitrite concentration of 1.5~2 mg/L.
Relationship of Optical Density and Wavelength on Light Source for Activated Sludge Concentration
Kim, Do-Han ; Lee, Chang-Han ; Wang, Bo-Kwon ; Suh, Jung-Ho ; Song, Seung-Koo ;
KSBB Journal, volume 25, issue 1, 2010, Pages 67~72
In this study, we developed a comprehensive correlation for better prediction of the activated sludge concentration in the relationship between measurement of optical density by the Beer's law and sludge properties. Effects of activated sludge properties, which are sludge types and anaerobic storage time, show that absorbance deviation for sludge properties decreased on wavelength of light source near to 950 nm. We also developed a method to measure limitation with non-linear correlation on the measurement of high concentration by the Beer's law using a double layer probe cell and empirical equations.
Field Test Assessment of Biological Recovering Agent for treating Oil Contaminated Soil
Kim, Soo-Hong ; Song, Seung-Koo ; Suh, Jung-Ho ;
KSBB Journal, volume 25, issue 1, 2010, Pages 73~78
In this study, recovering agent was produced with organic sludge and modified peat moss (MPM) in pilot plant mixer to recover oil contaminated soil, and field test of it was estimated using landfarming method. Oil contaminated soil recovering agent was thought to contain more microorganisms than raw waste sludge and was no problem to come onto the market because there were not any items of specified wastes. According to the results of TPH variation with time, it was observed the initial degradation velocity of oil with produced recovering agent was rapid up to 50% after 4 days, remarkably. Because the microorganisms in the organic sludge discharged from chemical plant already acclimated with oil, therefore, it could be estimated initial degradation velocity of recovering agent might be rapid. It was concluded that the oil contaminated soil recovering agent produced in this study have high marketability because of its two aspects on recycling of wastes and initial rapid degradation capacity.
Production of Invertase from Newly Isolated Strain Bacilus flexus
Oh, Tae-Seok ; Yun, Hee ; Sim, Ye-Ji ; Kim, Jin-Woo ; Choi, Min-Ji ; Yun, Jong-Won ;
KSBB Journal, volume 25, issue 1, 2010, Pages 79~84
In the present study, we isolated a new bacterial strain producing invertase (EC 126.96.36.199) and determined optimized culture condition in flask culture. The strain was identified as Bacilus flexus determined by the 16S rDNA sequencing method. The invertase was produced only in the sucrose medium as the sole carbon source. Potassium nitrate was an adequate nitrogen source for enzyme production, whereas meat peptone showed the highest bacterial growth. Enzyme production was increased about 2-fold when
was supplemented to the growth media. The optimum temperature was found to be
for both enzyme production and bacterial growth. Invertase exhibited pH optima in the range 5.0-6.0 and have a temperature optimum at
, similarly to other invertases found from different microbial sources. Several mineral ions (K and Fe) stimulated the invertase activity, whereas some bioelements (Ag, Mg, and Mn) inhibited enzyme activity. Under the optimized culture condition, the maximum enzyme production (over 250 units/mL) was achieved at 20 h. To the best of our knowledge, this is the first time to report on invertase production by Bacilus flexus.
Monitoring of Nitrogen Ion in Nitrifying Biofilm using an Ion Selective Microelectrode
Seon, Ji-Yun ; Byun, Im-Gyu ; Lee, Tae-Ho ; Park, Tae-Joo ;
KSBB Journal, volume 25, issue 1, 2010, Pages 85~90
An ion selective microelectrode (ISME) was fabricated to measure concentrations of ammonium (
-N) and nitrate (
-N) according to the depth of nitrifying biofilm. The limits of detectability and validity of results were investigated to evaluate the ISME. The electromotive force (EMF) was directly proportional to the ion concentration, and average detection limits of
M, respectively. The concentrations of
-N in various depths on the nitrifying biofilm were measured by the ISME. When a modified Ludzack-Ettinger (MLE) process was operated at an HRT of 6 h, concentration gradients of
-N in the bulk solution and biofilm at depths of
-N increased by
and remained constant thereafter. At an HRT of 4 h, concentration gradients of
-N at depths of
-N increased by
and remained constant thereafter. As HRT decreased, the concentration gradients of
-N between bulk solution and biofilm was higher due to the increase of nitrogen load. Also, the concentration gradients of the
-N of biofilm in the second aerobic tank were lower than those of the first aerobic tank, suggesting differences of nitrogen load and concentrations of DO between the first and second aerobic tank.
Concentration of Heavy Metals in Granule, Globular and Liquid types of Herbal Medicines
Bae, Jong-Sup ; Park, Jong-Pil ; Kim, Yong-Ung ; Park, Moon-Ki ;
KSBB Journal, volume 25, issue 1, 2010, Pages 91~96
This study is an endeavor to evaluate the safety of medicines from heavy metals, prescribed on the basis of herbal medicinal system and oriental medical prescription which are circulated much recently. For that, six globular types, six granular types and seven liquid types of herbal medicines were bought to compare and analyze the content of heavy metals, such as As, Pb, Cd and Hg, which are harmful to human body. The concentration of As was 0.219 mg/kg to 1.243 mg/kg, Cd was 0.0282 mg/kg to 0.8481 mg/kg, Pb was 0.9582 mg/kg to 5.233 mg/kg and Hg was 0.001 mg/kg to 0.01 mg/kg in globular and granular types. Otherwise, in the liquid types, As was 0.0123 mg/kg to 0.5024 mg/kg, Cd was 0.0128 mg/kg to 0.0568 mg/kg, Pb was 0.1755 mg/kg to 0.712 mg/kg, and Hg was 0.001 mg/kg to 0.002 mg/kg. It was found that the concentration of heavy metals in liquid types herbal medicines was relatively lower than globular and granular types. It is required to treat, manufacture and manage herbal medicines for safety.
Preparation of Thiol-chitosan Coated EPA-containing Liposome and Immune Response in Mouse Model of Atopic Dermatitis
Jung, Hyo-Yun ; Kim, Jin ; Lee, Yong-Wook ; Lee, Ki-Young ;
KSBB Journal, volume 25, issue 1, 2010, Pages 97~102
The enhancement of immunity for atopic dermatitis with application of eicosapentaenoic acid (EPA)-loaded liposome was evaluated on NC/Nga mice. The EPA-loaded liposome was coated with thiol-chitosan. The liposomes were characterized with transmission electron microscopy (TEM), surface zeta potential & particle size analyzer (Zeta-PSA) and differential scanning calorimetry (DSC). The loading efficiency of EPA in the liposome was about 4.7%. The particle size of the EPA-Ioaded liposome was about 230 nm. The values of Immunoglobulin E (IgE), interleukin-4 (IL-4), and tumor necrosis factor-
) were reduced significantly with application of the EPA-loaded liposome. The interferon-
) value was increased with the application effect. It is concluded that EPA loaded liposome have immunity advancing effects in mouse model of atopic dermatitis.
Development of Degradation Agent for Oil Contaminated Soil using Modified Peat Moss and Organic Sludge
Kim, Soo-Hong ; Lee, Chang-Han ; Suh, Jung-Ho ;
KSBB Journal, volume 25, issue 1, 2010, Pages 103~107
Oil degradation agent was developed with organic sludge and modified peat moss (MPM) to recover oil contaminated soil. Waste sludge discharged from wastewater treatment plant of chemical plant in Ulsan National Industrial Park was used as organic sludge, and MPM was purchased. Organic sludge was adequate to use as growth medium for microorganism, the surface of MPM had porous structure which could enhance the cultivation condition of oil degradation microorganisms. Water contents and TPH variation with time were observed to investigate the degradation capacity of developed degradation agent. Water contents were rapidly decreased with higher contents of MPM, however, in case of TPH, high MPM content decreased the degradation capacity. Therefore, it was recommended that the content of MPM was controlled to below 10% in degradation agent as mixing organic sludge with MPM.