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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 25, Issue 6 - Dec 2010
Volume 25, Issue 5 - Oct 2010
Volume 25, Issue 4 - Aug 2010
Volume 25, Issue 3 - Jun 2010
Volume 25, Issue 2 - Apr 2010
Volume 25, Issue 1 - Feb 2010
Selecting the target year
Biodiesel Production Using Microalgal Marine Biomass
Jo, Byung-Hoon ; Cha, Hyung-Joon ;
KSBB Journal, volume 25, issue 2, 2010, Pages 109~115
The demand of biodiesel that is a renewable, alternative fuel for fossil-based petrodiesel seems to keep increasing. Exploiting lipids of microalgae as a raw material for biodiesel is already technically feasible. To realize economical production of microalgal biodiesel, several factors or strategies should be addressed and improved. Especially, researches on improvement of lipid synthesis by genetic or metabolic engineering are now in early stage, and prospects of this field are bright, requiring concerns and interests of many researchers to put practical use of microalgal biodiesel forward.
Technology for Skin Rejuvenation and Homeostasis by Fermented Product with Micro-needle Therapy System
Kim, Eun-Ju ; Jung, Hyun-Ki ; Kim, Sung-Jun ;
KSBB Journal, volume 25, issue 2, 2010, Pages 116~122
Fermented materials have been used for long time around the world and have been researched according to the excellent effect in the part of medical and food industry. However, when such materials are applied on skin, because of the skin barrier, the most effective ingredients are poorly absorbed. The absorption of the skin is exceedingly limited and the method of increasing skin absorption needs special procedures. The micro-needle therapy is a method used to improve the absorption of drug (solution) in the skin which is called "natural skin rejuvenation therapy". This therapy uses micro-needle which is equipped with very thin, delicate needles smaller than a 0.07 mm thick hair. During this therapy, the micro-needle makes small holes and helps absorb the solution into the skin. This is a very excellent therapy in skin absorption. It can be used in wide regions of the skin without any side effects and no recovery time. In 2007, the micro-needle is permitted to personal care. However, the solutions have not yet been developed professionally, and such skill is needed.
Antifungal Activity of Bacillus sp. BCNU 2002 against the Human Pathogens
Choi, Hye-Jung ; Ahn, Cheol-Soo ; Jeong, Young-Kee ; Kim, Dong-Wan ; Joo, Woo-Hong ;
KSBB Journal, volume 25, issue 2, 2010, Pages 123~129
An endospore-forming, rod-shaped bacterium was isolated from forest soil samples collected at the Taebaek mountain of Gangwon province, Korea, and taxonomically characterized by physiological, biochemical and phylogenetic methods. Its 16S rRNA sequences showed the maximum similarity of 97% with B. amyloliquefaciens. In addition, the isolate BCNU 2002 was determined to have the ability to produce enzymes such as amylase, protease, gelatinase and catalase. The in vitro antifungal activity of Bacillus sp. BCNU 2002 was also examined against human pathogenic fungi such as Aspergillus niger, Candida albicans, Epidermophyton floccosum, Saccharomyces cerevisiae, Trichophyton mentagrophytes and Trichophyton rubrum. A maximum production level of antifungal substances of Bacillus sp. BCNU 2002 was achieved under aerobic incubation at
for 7 days in LB broth. BCNU 2002 showed strong antifungal activities against T. mentagrophytes and T. rubrum with the range of percentage inhibition from 56.25 to 63.23%. It was also confirmed that ethylacetate extract of cultured broth showed a strong antifungal activity against A. niger, C. albicans, S. cerevisiae and T. rubrum by agar diffusion method. The peptide fraction also exhibited broad antifungal spectrum against various pathogenic fungi. The minimum inhibitory concentration values for active extracts ranged between 125
/mL and 1000
Stabilization of Astaxanthin Using Nanoliposome
Yoo, Ji-Min ; Kim, So-Young ; Cho, Eun-Ah ; Cho, Eun-Hye ; Choi, Sun-Ju ; Jeong, Yoon-Joo ; Ha, Byung-Jhip ; Chae, Hee-Jeong ;
KSBB Journal, volume 25, issue 2, 2010, Pages 130~136
Astaxanthin is an unsaturated compound with a double bond. So it is easily decayed by heat and oxidation (light) during its storage and processing of it. Nanoliposome formulation technology was utilized to improve the stability of astaxanthin. Nanoliposome preparation conditions were established and the stability of astaxanthin encapsulated nanoliposome and free astaxanthin was investigated. Thermal stability and UV-stability of astaxanthin encapsulated nanoliposome increased up to two times and tree times, respectively. Astaxanthin encapsulated nanoliposome could be used as a stable functional material for industrial purposes.
Development of Coating Process of Citric Acid Using Vegetable Oil
Kim, Bok-Hee ; Kim, Dong-Man ; Lee, Sang-Hwa ; Shin, Hyun-Jae ;
KSBB Journal, volume 25, issue 2, 2010, Pages 137~141
Citric acid is a natural preservative and is used to add a sour taste to foods and soft drinks. For the preparation of stable food additives of citric acid, a coating process using vegetable oil was developed. Coating materials used were purified soy bean oil and carnauba wax. The yield of coated citric acid was up to 95% in both cases. The contents of coating materials was 20~33% in the total composition and the coating efficiency was 95.2
0.01%. The surface of coated citric acid was much smoother and more homogeneous than that of original citric acid according to SEM data. The coated citric acid can be used to as a stable food additive and also would be applied to nutraceuticals and cosmetic ingredients.
Statistical Optimization of Solid Growth-medium for Rapid and Large Screening of Polysaccharides High-yielding Mycelial Cells of Inonotus obliquus
Hong, Hyung-Pyo ; Jeong, Yong-Seob ; Chun, Gie-Taek ;
KSBB Journal, volume 25, issue 2, 2010, Pages 142~154
The protein-bound innerpolysaccharides (IPS) produced by suspended mycelial cultures of Inonotus obliquus have promising potentials as an effective antidiabetic as well as an immunostimulating agents. To enhance IPS production, intensive strain improvement process should be carried out using large amount of UV-mutated protoplasts. During the whole strain-screening process, the stage of solid growth-culture was found to be the most time-requiring step, thus preventing rapid screening of high-yielding producers. In order to reduce the cell growth period in the solid growth-stage, therefore, solid growth-medium was optimized using the statistical methods such as (i) Plackett-Burman and fractional factorial designs (FFD) for selecting positive medium components, and (ii) steepest ascent (SAM) and response surface (RSM) methods for determining optimum concentrations of the selected components. By adopting the medium composition recommended by the SAM experiment, significantly higher growth rate was obtained in the solid growth-cultures, as represented by about 41% larger diameter of the cell growth circle and higher mycelial density. Sequential optimization process performed using the RSM experiments finally recommended the medium composition as follows: glucose 25.61g/L, brown rice 12.53 g/L, soytone peptone 12.53 g/L,
5.53 g/L, and agar 20 g/L. It should be noted that this composition was almost similar to the medium combinations determined by the SAM experiment, demonstrating that the SAM was very helpful in finding out the final optimum concentrations. Through the use of this optimized medium, the period for the solid growth-culture could be successfully reduced to about 8 days from the previous 15~20 days, thus enabling large and mass screening of high producers in a relatively short period.
Strain Improvement through Protoplast Formation and Mutation of Inonotus obliquus Mycelia for Enhanced Production of Innerpolysaccharides (IPS) in Suspended Mycelial Cultures
Hong, Hyeong-Pyo ; Jeong, Yong-Seob ; Chun, Gie-Taek ;
KSBB Journal, volume 25, issue 2, 2010, Pages 155~166
Studies on the production of cell-wall bound innerpolysaccharides (IPS) (soluble
-D-glucan) have been performed by use of suspended myelial cultures of Inonotus obliquus. This product has promising potentials as an effective antidiabetic as well as an immunostimulating agents. As a first step to enhanced production of IPS, Intensive strain improvement programs were carried out by obtaining a large amounts of protoplasts for the isolation of single cell colonies. Rapid and large screening of high-yielding producers was possible because about fivefold higher amount of protoplasts (
protoplasts/mL) could be recovered with relatively high regeneration rates of
by applying a modified filtration method, as compared to the previously used trapping method. A basic protocol necessary for UV-mutation of the protoplasts was also developed, resulting in several overproducing variants with good fermentation properties. Since the amount of IPS extracted from the mycelial cell walls of I. obliquus turned out to be almost constant per g DCW, increase in cell mass was considered the most important factor for the enhancement in IPS production. Therefore, attempts were made to screen mutant cells showing rapid mycelial growth rate in the final suspended cultures. Notably, the mutant strains showing an active cellgrowth in the preceding solid growth cultures were observed to produce higher amount of IPS in the suspended fermentations as well. A striking mutant, OBLQ756-15-5 strain, obtained from the survivors of a harsh UV-treated condition (97% death rate) was found to stably produce as high cell mass as 22 g DCW/L in the final fermentations. Currently, this strain is being tested for development of a scaled-up fermentation process for mass production of IPS.
Construction of Transformation Method for Streptomyces scabiei ATCC 49173 Producing Phytotoxin
Jang, Bo-Youn ; Ha, Heon-Su ; Choi, Sun-Uk ;
KSBB Journal, volume 25, issue 2, 2010, Pages 167~172
Streptomyces scabiei producing phytotoxin called thaxtomin, which cause scab disease on economically important crops such as potato. For molecular genetics study of S. scabiei an effective transformation method was established based on conjugal transfer from Escherichia coli ET12567 (pUZ8002) using a phiC31-derived integration vector, pSET152, containing oriT and attP fragments. The high frequency was obtained on MS medium containing 50 mM
. In addition, the sequence and location of the chromosomal integration attB site of S. scabiei was identified for the first time in the strains producing thaxtomin by the southern blot analysis of exconjugants and the sequencing of plasmid containing DNA flanking the insertion sites from exconjugant chromosome. Similar to the case of Streptomyces species, a single phiC31 attB site of S. scabiei is present within an ORF encoding a pirin-homolog.
Antifungal Effect of Bilobalide and Ginkgolide Extracted from Leaves of Ginkgo biloba Against Pityrosporum ovale
Lee, In-Hwa ; Kim, Mi-Jin ; Choi, Jun-Ho ; Kim, Chi-Hyun ; Choi, Seung-Hyun ;
KSBB Journal, volume 25, issue 2, 2010, Pages 173~178
Antifungal effect of Ginkgo biloba leaves extracts conducted for Pityrosporum ovale. Antifungal effect verified by diffusion test, optical density test and colony counting test under various concentration. Extract of ginkgo biloba leaves performed with 40% ethanol and 60% water solution at
and major components analyzed by HPLC. The concentrated extract have bilobalide and ginkgolide A and ginkgolide B and their concentration were 153.0 mg/L, 8403.5 mg/L and 2723.0 mg/L respectively. Ginkgo biloba leaves extracts gave 99.1% of antifungal effect for Pityrosporum ovale examined by colony counting method.
Optimal Strategy for Ethanol Production in Repeated Fed-batch Operation Using Flocculent Sacchromyces cerevisiae
Lee, Sang-Eun ; Yeon, Ji-Hyeon ; Seo, Yong-Chang ; Kang, Do-Hyung ; Lee, Hyeon-Yong ; Jung, Kyung-Hwan ;
KSBB Journal, volume 25, issue 2, 2010, Pages 179~186
We investigated the optimal strategy for ethanol production using flocculent Sacchromyces cerevisiae ATCC 96581. Considering the characteristic of flocculent yeast, a repeated fed-batch ethanol fermentation was designed, in which non-sterile glucose powder was fed every 12 hours and, after cell flocculation, new feeding medium was exchanged every 24 or 36 hours. We particularly compared this fermentation process with those when cell flocculation was not carried out. Finally, the maximal total ethanol production was 825 g-ethanol during 120 hours, in which the time interval of withdrawal-fill of feeding medium was 24 hours and cell flocculation was carried out.
Operational Strategy for Increasing Ethanol Production in Repeated Fed-batch Ethanol Fermentation Using Saccharomyces cerevisiae
Lee, Sang-Eun ; Seo, Hyeon-Beom ; Kwon, Min-Cheol ; Lee, Hyeon-Yong ; Jung, Kyung-Hwan ;
KSBB Journal, volume 25, issue 2, 2010, Pages 187~192
We designed the optimal operational strategy in repeated fed-batch ethanol fermentation using Sacchromyces cerevisiae ATCC 24858 in views of ethanol yield, specific ethanol production rate, and ethanol productivity, when the aeration rate were controlled at 0.0 and 0.33 vvm. Coincidentally, the time intervals of withdrawal-fill of culture medium (24 and 36 h) were investigated. Ethanol yield and ethanol productivity when the aeration was carried out at 0.33 vvm were superior to those when the aeration was not carried out. Additionally, those parameters when the time interval of withdrawal-fill of culture medium was 24 h were superior to those when time interval of withdrawal-fill of culture medium was 36 h. The total ethanol production reached at the greatest value, 703.8 g-ethanol, when the aeration was carried out at 0.33 vvm and the time interval of withdrawal-fill of culture medium was 24 h. In this study, we verified experimentally the necessity of designing the operational strategy for increasing ethanol production in terms of aeration rate and time interval of withdrawal-fill of culture medium in the repeated fed-batch ethanol fermentation.
Effects of Jasmonic Acid-Cellulase Combined Elicitors on the Paclitaxel Production in Suspension Cultures of Taxus wallichiana Zucc
Hoi, Nguyen Ngoc ; Luong, Hoang Van ; Long, Nguyen Van ; Duong, Vu Binh ; Byun, Sang-Yo ;
KSBB Journal, volume 25, issue 2, 2010, Pages 193~198
Cell cultures of Taxus wallichiana Zucc. were made to enhance the production of anticancer agent paclitaxel. In suspension cultures, the maximum cell growth rate in exponential growth phase was 0.14
which was correlated to 4.96 days of cell doubling time. The production of paclitaxel was non-growth associated. The paclitaxel production was started after the exponential growth phase and increased to declined phase where the maximum concentration was observed. Various elicitors were tested to enhance the production of paclitaxel. The combination of two elicitors of jasmonic acid and cellulase increased the production of paclitaxel 1.8 and 3.1 times compared to paclitaxel production by individual elicitor respectively.
Production of Repetitive Polypeptides for an Efficient DNA Analysis on a Microchip
Yi, Hyeon-Jin ; Choi, Seok-Jin ; Seo, Tae-Seok ; Won, Jong-In ;
KSBB Journal, volume 25, issue 2, 2010, Pages 199~204
We generated the feasibility of DNA separation in free-solution using genetically engineered repetitive polypeptides as drag-tags. Two different-sized repetitive polypeptides were designed, expressed in E. coli, and purified. They were conjugated to a fluorescently labeled DNA (100 base), and the electrophoretic mobilities of these conjugate molecules were analyzed on a microchip. The results of these studies indicate that genetically engineered repetitive polypeptide is a prominent candidate for rapid and high-throughput genetic mutation detection, such as SNP analysis.