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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 25, Issue 6 - Dec 2010
Volume 25, Issue 5 - Oct 2010
Volume 25, Issue 4 - Aug 2010
Volume 25, Issue 3 - Jun 2010
Volume 25, Issue 2 - Apr 2010
Volume 25, Issue 1 - Feb 2010
Selecting the target year
Isolation and Purification of Bioactive Materials Using High-Performance Counter-Current Chromatography (HPCCC)
Jung, Dong-Su ; Shin, Hyun-Jae ;
KSBB Journal, volume 25, issue 3, 2010, Pages 205~214
Many successive liquid-liquid extractions occur enabling purification of the crude material to occur. In high performance counter-current chromatography (HPCCC), crude material is partitioned between two immiscible layers of solvent phases. The stationary phase (SP) is retained by hydrodynamic force field effect and the mobile phase (MP) is pumped through the column. Purification occurs because of the different solubility of the components in the liquid mobile and stationary phases. There are many key benefits of liquid stationary phases such as high mass and volume injection loadings, total sample recovery, and easy scale-up. Many researchers showed that predictable scale-up from simple test is feasible with knowledge of the stationary phase retention for the planned process scale run. In this review we review the recent advances in HPCCC research and also describe the key applications such as natural products and synthetics (small or large molecules).
Trends in Protein Engineering for Gene Targeting: Homing Endonucleases and Zinc Finger Nucleases
Cheong, Dea-Eun ; Kim, Geun-Joong ;
KSBB Journal, volume 25, issue 3, 2010, Pages 215~222
Monogenic diseases are resulted from modifications in a single gene of human cells. Because their treatment with pharmacological medicine have a temporary effect, continuous nursing care and retreatment are required. Gene therapy, gene targeting and induced pluripotent stem cell (iPSC) are considered permanent treatment methods of them. In gene therapy, however, retroviral vectors that have potential toxicity caused by random insertion of harmful virus are used as vehicles for transferring genetic materials. On the other hand, gene targeting could replace and remove the modified gene though homologous recombination (HR) induced by site-specific endonucleases. This short review provides a brief overview on the recently tailored endonucleses with high selectivity for HR.
Production of Fucoidan Using Marine Algae
Park, Keun-Hyoung ; Cho, Eun-Hye ; Kim, Nam-Chan ; Chae, Hee-Jeong ;
KSBB Journal, volume 25, issue 3, 2010, Pages 223~229
Fucoidans, polysaccharides that are composed of fucose, uronic acids, galactose and xylose, are one of the main constituents of brown seaweeds. For the past decade algal fucoidans have been extensively studied due to their biological activities including anticoagulant, antiinflammatory, antitumor, contraceptive and antiviral activities. Even though fucoidan is known as a highly functionality derivative, its industrial applications have been limited because of low inclusion efficiency and high cost of manufacturing. Furthermore the sea-weed smell of fucoidan has been a limiting factor for the application especially in food and cosmetic area. The potential application of various extraction technology and deodorization of sea-weed smell is highly required for a wider application of fucoidan. As an alternative extraction process, enzymatic hydrolysis process or ultra-high pressure treatment has been investigated for the improvement of production yield and bioefficacy of fucoidan. More intensive research on the fucoidan production technology and its application in nutraceutical and cosmoceutical area is needed.
Optimum Conditions for the Production of Keratinase by Bacillus sp. KN-517 and Application to the Degradation of Hair
Kim, Hye-Sook ; Shim, Kyu-Nam ; Kang, Sang-Mo ;
KSBB Journal, volume 25, issue 3, 2010, Pages 230~238
A microbial strain having high keratinase activity was isolated from the soil of poultry factories of Gyeonggi or Chungcheong-do. The isolated strain was identified as Bacillus sp. based on its morphological and biochemical characteristics. In this study, the optimal conditions for the production of keratinase by this strain were investigated. The optimal medium composition for the keratinase production was determined to be 3.5% chicken feather as carbon source, 1.0% tryptone as organic nitrogen source, 1.0%
as inorganic nitrogen source and 0.05% KCl, 0.05%
as mineral source and 0.01% yeast extract as growth factor. The optimal temperature and pH was
and 8.5 with shaking culture (200 rpm), respectively. The maximum keratinase production reached to 123 units/ml after 42 hr of cultivation under the optimal condition. When the hair was used as the sole carbon source, the maximum enzyme activity was 88 units/ml after 120 hr and in this case, the hair added in the medium was not degraded completely but got thinner than the control by 20%.
Characteristics of Polyhydroxyalkanoates Synthesis by Ralstonia eutropha from Vegetable Oils
Park, Dae-Hoo ; Kim, Beom-Soo ;
KSBB Journal, volume 25, issue 3, 2010, Pages 239~243
Six strains of Ralstonia eutropha were grown to investigate characteristics of polyhydroxyalkanoates (PHA) synthesis from vegetable oils or glycerol. Poly(3-hydroxybutyrate) homopolymer was formed using soybean oil, olive oil, or glycerol as carbon source, while poly(3-hydroxybutyrate-co-4-hydroxybutyrate) or poly(3-hydroxybutyrateco-3-hydroxyvalerate) copolymers were synthesized by co-feeding
-butyrolactone or pentanoic acid, respectively. Optimum strain was determined as R. eutropha KCTC 2662 in terms of final cell concentration and PHA content. From 20 g/L of soybean oil (optimum substrate), cell concentration and PHA content at 72 h ranged 1.7~9.2 g/L and 70~92 wt%, respectively.
Characterization and Anti-Helicobactor pylori Activity of Xanthium strumarium L. Extract on Lactic acid Fermentation
Kang, Dong-Hee ; Kim, Hyun-Soo ;
KSBB Journal, volume 25, issue 3, 2010, Pages 244~250
This study characterized the anti-Helicobactor pylori activity of Xanthium strumarium L. extract obtained by lactic acid fermentation. The growth of the Lactobacillus strains was typically robust upon lactic acid production in monocultures containing Xanthium strumarium L. extract. Lactic acid fermentation in mixed cultures of Lactobacillus brevis KCTC 3498 and Lactobacillus casei KCTC 3109 produced higher of anti- H. pylori activity than monocultures. Concerning antioxidant activity of fermented extracts, total polyphenol contents were the highest in co-cultures of Lactobacillus brevis KCTC 3498 and Lactobacillus helveticus KCTC 3545. Electron donating ability using diphenyl picryl hydrazyl (DPPH) showed 70% scavenging in Lactobacillus casei KCTC 3109.
Wastepaper Liquefaction Using Ethylene Glycol and Polyester Preparation from the Liquefied Wastepaper
Lee, Dong-Hun ; Kim, Chang-Joon ; Kim, Sung-Bae ;
KSBB Journal, volume 25, issue 3, 2010, Pages 251~256
A novel method to prepare polyester from wastepaper through liquefaction and crosslinking stages was studied. At the first stage, the liquefaction of wastepaper was carried out in the presence of ethylene glycol under acidic conditions. The factors that affect on liquefaction yield were found to be reaction time, temperature, and acid concentration, and their ranges were 60~120 minutes,
, and 2~4%, respectively. The optimum condition was found to be 100 minutes,
, and 3% sulfuric acid concentration, and the liquefaction yield at this condition was 67%. At the second stage, polyester was prepared from the liquefied wastepaper obtained at the optimum liquefaction condition by crosslinking with succinic anhydride. The effect of reaction time and carboxylic group/hydroxyl group ratio on crosslinkage were investigated at conditions covering 30~50 minutes of reaction time and 1.5~2.5 of carboxylic group/hydroxyl group ratio. The crosslinkages of polyester prepared were 80~90%, which were almost same regardless of reaction conditions.
Expression and Purification of Recombinant Human Bone Morphogenetic Protein-7 (rhBMP-7) in Bacillus subtilis
Kim, Chun-Kwang ; Oh, Sung-Duk ; Rhee, Jong-Il ;
KSBB Journal, volume 25, issue 3, 2010, Pages 257~264
Bone morphogenetic protein-7 (BMP-7) is one of important growth factors for skeletal development and bone growth. In this work, BMP-7 was efficiently expressed in recombinant Bacillus subtilis. The mature BMP-7 protein indicated molecular weight of 15.4 kDa by Western blot assay and was secreted into culture medium with 0.35 ng/mL. The extracellular and intracellular rhBMP-7 proteins were purified by using a FPLC system with an ion exchange column and a gel filtration column. The extracellular and intracellular rhBMP-7 proteins had finally a 57.1% purity and a 36.2% purity, respectively. The purified rhBMP-7 proteins showed an intact biological activity which stimulated alkaline phophatase (ALP) activity in MC3T3-E1 cells.
Effect of Simvastatin on the Migration and Invasion of U-373-MG Cells
Kim, Hwan-Gyu ;
KSBB Journal, volume 25, issue 3, 2010, Pages 265~270
Simvastatins are widely used to reduce endogenous cholesterol synthesis and improve hypercholesterolemia. Also, simvastatin have been shown to induce both angiogenic and angiostatic responses. In this study, It was attempted to resolve this controversy by studying the effects of simvastatin on the cell migration and invasion with the proteinases secretion and expression pattern. U-373-MG cells treated with low dose of simvastatin (
) showed the induction of migration and invasion compared with the addition of a control buffer. On the contrary, high dose of simvastatin (
) showed the reduction of migration and invasion compared with the addition of a control buffer. It was also showed that simvastatin-regulated migrative and invasive phenotypes were consistent with the secretion and expression pattern of matrix metalloproteinase-2 (MMP-2), MMP-9 and plasmin.
Analysis of Isoflavonoid Contents in Astragalus membranaceus Bunge Cultivated in Different Areas and at Various Ages
Im, Kyung-Ran ; Kim, Mi-Jin ; Jung, Teak-Kyu ; Yoon, Kyung-Sup ;
KSBB Journal, volume 25, issue 3, 2010, Pages 271~276
This study was carried out to obtain the basic information for isoflavonoid contents that can be used to index Astragalus membranaceus B. cultivated in the Republic of Korea and China. Isoflavonoid contents in Astragalus membranaceus B. which were cultivated in various areas (Jecheon, Jeongseon, Yeongju, and Taebaek in Korea, and China) and ages (1-year-old, 3-years-old) were determined. Calycosin and formononetin as major constituents were determinated by HPLC method in Astragalus membranaceus B. The results show that there were no statistically significant differences for the average contents of isoflavonoids among 1-year-old and 3-years-old. However, isoflavonoid contents were significant differences according to the cultivation areas. HPLC analysis showed that the calycosin content of 1-year-old at Jeongseon was the highest level of
and that of 1-year-old at Yeongju was the lowest level of
. The highest level of formononetin content was
of 1-year-old at China, while the lowest level was
of 1-year-old at Yeongju. These results strongly suggest that contents of isoflavonoid in Astragalus membranaceus B. might be quite different with respect to the cultivation areas.
Characteristics and Biological Properties of Pleurotus eryngii grown on Monosodium Glutamate-enriched Media
Yoon, Dong-Yeon ; Park, Ki-Moon ; Lee, Jae-Heung ;
KSBB Journal, volume 25, issue 3, 2010, Pages 277~282
This study was performed to investigate the composition of amino acids and biological properties with the ethanol extract of fruiting bodies of Pleurotus eryngii grown on the sawdust compost mix (400 g sawdust plus 200 g rice bran) supplemented with various dosages of monosodium glutamate (MSG). Amino acid composition analyses showed that arginine, glutamic acid, alanine and glycine increased as the dosage of MSG was increased, whereas histidine, serine, methionine, isoleucine, leucine and phenylalanine did not increase.
-Aminobutyric acid (GABA) content increased significantly up to 1.18 mg/g extract when 6 g MSG was supplemented to the sawdust mix. Antioxidant activity of the extract was estimated and compared to the standard antioxidant (ascorbic acid). The antioxidant property such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity increased with the extract samples of increasing dosage of MSG. Although the extract showed low levels of nitrite scavenging activity, this activity increased up to 1.5-2.0 fold when MSG was supplemented to the sawdust mix above a dosage of 0.5 g. The results obtained from the present investigation would appear that Pleurotus eryngii grown on the MSG-enriched sawdust mix can be used more effectively as one of potential sources of functional foods.
Bioethanol Production from Hydrolysate of Seaweed Sargassum sagamianum
Yeon, Ji-Hyeon ; Seo, Hyeon-Beom ; Oh, Sung-Ho ; Choi, Won-Seok ; Kang, Do-Hyung ; Lee, Hyeon-Yong ; Jung, Kyung-Hwan ;
KSBB Journal, volume 25, issue 3, 2010, Pages 283~288
We investigated the feasibility of bioethanol production from hydrolysate of brown seaweed Sargassum sagamianum. Prior to bioethanol production using yeasts, six yeast strains were compared and the best ones in terms of the ethanol production levels were selected. Pichia stipitis ATCC 7126, Pichia stipitis ATCC 58784, and Pichia stipitis ATCC 58376 were superior to others in terms of ethanol production. These yeast strains were used for producing bioethanol by the shaking bottle culture and the fermentor culture. Out of approximately 30 g/L reducing sugar, about 3~6 g/L and 4~7 g/L bioethanol were produced in the bottle culture and the fermentor one, respectively. Furthermore, it was observed that around 12~28 g-bioethanol was produced from 1 kilogram of Sargassum sagamianum. Compared with those previously published, these data were almost three to eight times higher in value.
Antifungal Activity and Biochemical Characterization of Lectin Isolated from Locular Fluid of Cherry Tomato Fruit
Roh, Kwang-Soo ;
KSBB Journal, volume 25, issue 3, 2010, Pages 289~296
Lectins are carbohydrate-binding and a cell-agglutinating proteins, and are concerted with a plants defence mechanism. In particular, chitin-binding lectins in locular fluid of cherry tomato fruit seemed to have a role in defending plants against fungi. The antifungal activity using lectin isolated from locular fluid of cherry tomato fruit was measured in the plant pathogen Cladosporium cucumerinum, Monosporascus cannonballus, Fusarium oxysporum, and Rhizoctonia solani. Amoung the four strains, a potent antifungal activity was detected in Cladosporium cucumerinum and Monosporascus cannonballus, not in Fusarium oxysporum, and Rhizoctonia solani. The molecular weight of this lectin isolated as double protein bands by SDS-PAGE was calculated to be 87 kDa and 47 kDa from the relative mobilities compared with those of reference molecular weight markers. The isolated lectin agglutinated human red blood cells (A, B, AB, O) treated with trypsin, and the most activity was found at B. The optimal temperature of isolated lectin was at
. For the thermal stability, lectin was stable at
. The optimal pH of this lectin was at 7.2, and showed complete loss below pH 9.0.
Preparation and Evaluation of Adhesive Hydrogel of Ketoprofen Using Microsphere System
Cho, Young-Ho ; Lee, Gye-Won ;
KSBB Journal, volume 25, issue 3, 2010, Pages 297~302
Ketoprofen is one of the propionic acid class of nonsteroidal anti-inflammatory drug with analgesic and antipyretic effects. The most common side effects from ketoprofen after oral administration are gastrointestinal irritation, diarrhea, abdominal pain and retention of fluid. Ketoprofen was formulated as water-soluble gels to reduce these side effects. To increase the skin permeability of ketoprofen, microsphere containing ketoprofen was prepared with chitosan and ploy-
-caprolactone. And then prepared microsphere was manufactured as an adhesive hydrogel with polyvinylpyrrolidone K-25, polyethylene glycol 4000, and various permeation enhancers. The flux and permeability of ketoprofen were evaluated. As the concentration of tween 80 and enhancers increased, the flux of ketroprofen was accelerated. Also the permeation rate was facilitated by enhancers, but did not affect the lag time. From these results, the adhesive hydrogel using microsphere could be a good delivery system for ketoprofen to improve the skin permeation.