Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal Basic Information
Journal DOI :
Korean Society for Biotechnology and Bioengineering
Editor in Chief :
Volume & Issues
Volume 25, Issue 6 - Dec 2010
Volume 25, Issue 5 - Oct 2010
Volume 25, Issue 4 - Aug 2010
Volume 25, Issue 3 - Jun 2010
Volume 25, Issue 2 - Apr 2010
Volume 25, Issue 1 - Feb 2010
Selecting the target year
Development of Probiotic Products and Challenges
Seo, Jae-Gu ; Lee, Gwa-Soo ; Kim, Jin-Eung ; Chung, Myung-Jun ;
KSBB Journal, volume 25, issue 4, 2010, Pages 303~310
Probiotics beneficially affect the health of the host via various mechanisms in the intestine. Recent developments in probiotic products have mainly been made to maximize probiotic effects in human. In this regard, probiotic products containing doubly coated or encapsulated cells, multi-species probiotics, or high viable cell number (1010 viable cells/gram or more) have been developed and are already available in the market. Until now, the majority of probiotics contain live cells but little attention has been paid to other alternative products such as heat-killed cell or bacteriocin-containing ones, which could have broad applications due to advantages over live cell-based probiotics, such as safety and stability. In addition, genetically engineered lactic acid bacteria could be of great importance in the field of alimentary health if they are carefully designed for biological safety. Although a number of probiotics are marketed by claiming health benefits, regulations for health claims will be more stringent. Therefore sufficient scientific and clinical evidences supporting the safety and efficacy of the potential probiotic strain will be required by the regulatory authority for a health claim, which thus may have a huge impact on the future probiotic market.
Current Strategies for Metabolic Engineering of Lactic Acid Bacteria
Han, Nam-Soo ; Cho, Seung-Kee ; Kim, Yu-Jin ;
KSBB Journal, volume 25, issue 4, 2010, Pages 311~318
Lactic acid bacteria display a relatively simple metabolism wherein the sugar is converted mainly to lactic acid. The extensive knowledge of metabolic pathways and the increasing information of the genes involved allows for the rerouting of natural metabolic pathways by genetic and physiological engineering. In this contribution, the lactic acid bacteria as an efficient cell factory for different (food) ingredients will be presented. The emphasis will be on some successful examples of metabolic engineering and on the physiology of these bacteria, which makes them so suitable as a cell factory.
Research Trends in Bifidobacterium
Park, Myeong-Soo ; Ji, Geun-Eog ;
KSBB Journal, volume 25, issue 4, 2010, Pages 319~329
Bifidobacteria comprises up to 25% of the cultivable fecal bacteria in adults and 80% in infants. Many in vivo and clinical research results supporting its efficacy in the prevention and improvement of gastrointestinal health have been accumulated. As a consequence, expert committee WHO/FAO expert committee recommended Bifidobacterium as representative probiotics together with Lactobacillus acidophilus. In this review, research trends in bifidobacteria concerning the classification and identification of the genus Bifidobacterium, modulation of intestinal microflora, improvement of constipation, prevention of diarrhea, alleviation of atopy and allergy, barrier function through antimicrobial activity andimmune enhancement of the host will be introduced. Several gene expression systems based on bifidobacterial plasmids have been developed and successfully used to express several heterologous genes including anticancer proteins in Bifidogacterium. In animal test, bifidobacteria was proven to be a promising candidate for safe gene delivery system which can specifically colonize in the solid tumor.
Melanin Biosynthesis Inhibitory Effect of New Compound (Jeju-Erythrane) Isolated from Bark of Lindera erythrocarpa Makino
Kang, Min-Chul ; Ko, Ryeo-Kyeong ; Kim, Su-Gyeong ; Choi, Ho-Min ; Jin, Yeong-Jun ; Han, Jong-Heon ; Kim, Bong-Seok ; Lee, Nam-Ho ; Kim, Gi-Ok ;
KSBB Journal, volume 25, issue 4, 2010, Pages 330~336
In this study, a new compound, 1-(2-hydroxy-3,4,5,6-tetramethoxyphenyl)-1-methoxy-3-phenylpropane; (Jeju-Erythrane) was isolated and identified from the bark of Lindera erythrocarpa Makino. Also, we investigated the effects of Jeju-Erythrane on alpha melanocyte-stimulating hormone (MSH)-induced melanogenesis in mouse B16F10 melanoma cells. The new compound dose dependently inhibited the tyrosinase activity and melanin synthesis in B16F10 cells. The new compound showed inhibitory effect on the Tyrosinase and TRP-1 gene transcription but not on the TRP-2 gene. These results suggest that the new compound of L. erythrocarpa could be used as a functional biomaterial in developing skin whitening agent.
Enzymatic Biodiesel Synthesis from Canola Oil in Liquid Carbon Dioxide
Lee, Myung-Gu ; Park, Chul-Hwan ; Cho, Jae-Hoon ; Lee, Jun-Hak ; Lee, Do-Hoon ; Kim, Sang-Yong ;
KSBB Journal, volume 25, issue 4, 2010, Pages 337~343
It has been well known that organic solvents like t-butanol and n-hexane can protect lipases from the inhibition by short-chain alcohols in the enzymatic transesterification. However, use of the organic solvents should be minimized considering their negative effects on environment and human health. Therefore, use of the greener solvents has been pursued in various are as including the enzymatic biotranformation. In this study, the liquid carbon dioxide (
) was employed as an alternative media for the enzymatic transesterification of canola oil. The conversion in the
was comparable with those in organic solvents and the supercritical carbon dioxide, and under optimum conditions, the value reached 99.7%. It is expected that this method can provide a new type of biodiesel production process with higher energy efficiency and lower environmental impact.
Chemotactic Cell Migration around Hollow Silica Beads Containing Chemotatic Reagent
Kim, Hae-Chun ; Kang, Mi-Seon ; Rhee, Seog-Woo ;
KSBB Journal, volume 25, issue 4, 2010, Pages 344~350
This paper demonstrates a microfluidic chip incorporating patterned hollow silica beads that can be effectively used for chemotaxis assay. The hollow silica bead has been exploited to develop a carrier for chemoattractant to induce cell migration. The microfluidic chip contains a patterned array of microfabricated docks which can hold only one bead per docking site. The hollow bead placed inside microfluidic chip releases chemotactic reagent (PDGF-BB) around its periphery in a controlled fashion which generates a signal for chemotatic migration of fibroblast cells. The number of cells migrated close to each bead has been assessed. On-chip cell migration assay showed a remarkable result proving the high efficiency and reliable accuracy in quantitative analysis. Therefore, the device could be extensively used in cell migration assay and other various studies related to cellular movements.
Ethanol Production with Glucose/Xylose Mixture by Immobilized Pichia stipitis
Shin, Hyun-Seok ; Kang, Seong-Woo ; Lee, Sang-Jun ; Jang, Eun-Ji ; Suh, Young-Woong ; Kim, Seung-Wook ;
KSBB Journal, volume 25, issue 4, 2010, Pages 351~356
To increase the production of ethanol by using sugar from lignocellulosic biomass, pentose and hexose have to be fermented simultaneously by yeast. The effects of mixed sugar and nitrogen on ethanol production by immobilized Pichia stipitis KCCM 12009 were investigated. When optimal mixed sugar and nitrogen concentration were 5% (Glucose/Xylose = 3:1) and 1%, respectively, ethanol concentration produced by immobilized P. stipitis was 19-20 g/L. In repeated fed-batch by immobilized P. stipitis, all glucose was consumed very quickly at 1-3% mixed sugar concentration. But, xylose consumption was decreased as the mixed sugar concentration increased. Also, ethanol (5.6 g/L) was stably produced and ethanol production rate was 0.13 g/
in immobilized cell reactor (ICR) with 1% mixed sugar (Glucose/Xylose = 3:1) as feeding media.
Enhancement of Saccharification Yield of Ulva pertusa kjellman for Ethanol Production through High Temperature Liquefaction Process
Han, Jae-Gun ; Oh, Sung-Ho ; Choi, Woon-Yong ; Kwon, Jung-Woong ; Seo, Hyeon-Beom ; Jeong, Kyung-Hwan ; Kang, Do-Hyung ; Lee, Hyeon-Yong ;
KSBB Journal, volume 25, issue 4, 2010, Pages 357~362
Green alga, Ulva pertusa kjelmann has been known to be one of the largest pollutants in Korea. Therefore, the efficient pretreatment processes have been required to improve the yields of fermentable sugar. The optimal pretreatment conditions were determined to be
for 15 min. The sugar yield of glucose and xylose were estimated as 20.5%, and 5.0% respectively, based on theoretical yields. However solid residues were estimated enzymatic digestibility of 90-95% with cellulase loading of 15 FPU/g glucan. This process was proved to generate the low concentration of Hydroxy-Methyl-Furfural (51 ppm), which resulted in ethanol production with 95% of the maximum conversion yield from glucose in the culture of Saccharomyces cerevisiae (ATCC, 24858). This study showed that Ulva pertusa kjellmann can be used as a bioetahnol resource using the high temperature liquefaction process.
Extraction, Purification and Property of the lipid from Scenedesmus sp.
Kim, Na-Young ; Oh, Sung-Ho ; Choi, Woon-Yong ; Lee, Hyeon-Yong ; Lee, Shin-Young ;
KSBB Journal, volume 25, issue 4, 2010, Pages 363~370
Lipid from Scenedesmus sp. was extracted, fractionated and purified by silicic acid column chromatography. Total lipid content of extract was
wt%, and triacylglycerol, an index lipid for biodiesel production was detected. Ten species of
with saturated and unsaturated fatty acid were identified showing the very adequate fatty acid profiles for biodiesel production with a good flow property at low temperature. The fractions of glycolipid, neutral and phospholipid were 52.64, 28.10 and 19.26% of total lipid, respectively. The triacylglycerol of 12.63% and chlorophyll a of 49.47% was fractioned using stepwise elution of n-hexane-diethyl ether (95:5, v/v) solvent. The high content of chlorophyll was considered as a potential source of value-added co-product.
Optimization of Lipid Extraction from Scenedesmus sp. Using Taguchi Approach
Kim, Na-Young ; Oh, Sung-Ho ; Choi, Woon-Yong ; Lee, Hyeon-Yong ; Lee, Shin-Young ;
KSBB Journal, volume 25, issue 4, 2010, Pages 371~378
For the biodiesel fuel production from microalgae, the lipid from wet and dry samples of green algae Scenedesmus sp. was extracted by using various solvents and pre-treatment methods. Extraction yield of the lyophilized sample was better than that of dry sample. Chloroform/methanol (2:1, v/v) and ultrasonication or homogenization method were also selected as the most effective solvent and pre-treatment methods for lipid extraction, respectively. Under these constraint conditions, optimization experiment of lipid extraction was investigated by Taguchi approach using orthogonal matrix
) method. The optimum extraction conditions of lipid extraction was obtained at pre-treatment of homogenization, extraction time of 5 hour, temperature of
, and solvent ratio of 1:20 (w/v). Yield of extraction at optimized condition was 20.55% and it was 96% of total lipid content (21.38%) of Scenedesmus sp.
Preparation of PEG Microparticles Containing Coriander Essential Oil Using Supercritical PGSS Process
Choi, Jin-Ah ; Lim, Gio-Bin ; Ryu, Jong-Hoon ;
KSBB Journal, volume 25, issue 4, 2010, Pages 379~386
In the present study, biocompatible poly (ethylene glycol) (PEG) microparticles containing coriander essential oil were prepared using a supercritical particles from gas saturated solution (PGSS) process to improve the stability of the coriander oil. The effects of various process parameters such as temperature, pressure, and nozzle diameter on the morphology and entrapment efficiency of coriander oil loaded PEG microparticles were then investigated. A positive influence on the formation of spherical microparticles was observed with increasing temperature and decreasing pressure. Furthermore, somewhat more porous microparticles were produced with an increase in pressure. At a given temperature, the highest entrapment efficiency of coriander essential oil in PEG microparticles was observed under the lowest experimental pressure condition.
Effect of Complex Extracts of Mushroom and Sharp Toothed Eel on the Skin Conditions with Atopic Dermatitis and Acne Symptoms
Cha, Wol-Suk ; Yoo, Ji-Hyun ; Min, Myung-Ja ; Nam, Hyung-Gun ; Kim, Yun-Soo ; Shin, Hyun-Jae ; Kim, Jong-Soo ; Choi, On-You ; Kim, Ran ; Choi, Du-Bok ;
KSBB Journal, volume 25, issue 4, 2010, Pages 387~394
This study was conducted to investigate effect of complex extract of various mushrooms and sharp toothed eel on the skin conditions with atopic dermatitis and acne symptoms. The total phenol concentration was increased in order of hot water> ethanol> ethyl acetate> petroleum ether> chloroform extract. Especially, when the hot water extract was used, it was about 2-3 fold higher than that of ethyl acetate, petroleumether, and chloroform extract. When the complex extract concentration was increased from 1.0 to 50 mg/L, the DPPH scavenging rate increased from 10.1 to 81.4%. The reduction power was sharply increased from 0.05 to 0.27 (700 nm) when the complex extract concentration was increased from 25 to 75 mg/L. However, above 100 mg/L, it was not decreased. In the case of SOD-like activity, it was 45.7% at 100 mg/L. Total numbers of patients with atopic dermatitis were 15 and 5 patients with severe acne symptoms. According to photos taken before and after the treatment and questionnaire results, considerable improvements in skin conditions are observed in the patients with atopic dermatitis and acne. For atopic patients, erythema and edema have been improved but the degree of effect was dependent on the individual's constitution. Concerning acne, the effect of coating of the extracts was prominent for first week and the degree decreased with time till 4 weeks. The complex extracts wereefficient in soothing rash and maturation. Side effects such as a scar were not detected during the application and treatment.
Glyceryl Esterification of Fibroin Peptide by Papain
Jeong, Jae-Ho ; Lee, Shin-Young ; Hur, Won ;
KSBB Journal, volume 25, issue 4, 2010, Pages 395~400
Papain hydrolysate of fibroin was found to be mainly composed of several even-numbered peptides that can be produced at a large scale and can be used as a precursor for biological fine-chemicals such as peptide detergents. Thus, the hydrolysate was further modified to synthesize a peptide mixture of glyceryl esters using the identical enzyme for the production of such chemicals. Formation of glyceryl ester of each peptide was confirmed by identifying peaks of the nominal mass shift of +74 Da in mass spectrometry. Analysis of the mass spectra indicated that glyceryl esters of di- and tetra-peptides were the major constituents of the mixture and that alanylglycine was most preferentially esterified. It also suggests that papain prefers dipeptide to tetrapeptide and alanine to serine or tyrosine at
position as substrate for glyceryl esterification. The glyceryl esters were recovered using ion exchange resin and the yield of glyceryl esterification recorded was 17.8% by weight.
Selection of Inhibitor-resistance Yeast and its Application to Bioethanol Production in the Hydrolysate of Rape Stem
Yeon, Ji-Hyeon ; Kim, Hye-Ji ; Oh, Sung-Ho ; Lee, Hyeon-Yong ; Jung, Kyung-Hwan ;
KSBB Journal, volume 25, issue 4, 2010, Pages 401~407
We established a strategy for bioethanol production using the hydrolysate of rape stem, in which the inhibitor cocktail was added intentionally. The final goal of this study was to circumvent the detoxification process when the hydrolysate of lignocelluloisic biomass contained the toxic substances in high concentration. When six yeast strains were examined, Sacchromyces cerevisiae ATCC 96581 and Pichia stipitis CBS 7126 were relatively resistant to inhibitor cocktail. Then, using strains 96581 and 7126, we designed a process strategy for bioethanol production, assuming that the concentration of toxic substance in the hydrolysate of rape stem was remarkably high. When strains 96581 and 7126 were inoculated simultaneously, it was observed that strain 7126 produced bioethanol as well as strain 96581, although the concentration of inhibitor cocktail was 18.2% (v/v). Finally, throughout this co-cultivation of strains 96581 and 7126, bioethanol was produced about 6.0 (g/L), and bioethanol yield reached at 0.4 (g-bioethanol/g-reducing sugar) (78.4% of theoretical value).