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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 25, Issue 6 - Dec 2010
Volume 25, Issue 5 - Oct 2010
Volume 25, Issue 4 - Aug 2010
Volume 25, Issue 3 - Jun 2010
Volume 25, Issue 2 - Apr 2010
Volume 25, Issue 1 - Feb 2010
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Development of Biopolymer-based Materials Using Ionic Liquids and Its Biotechnological Application
Lee, Sang-Hyun ; Park, Tae-Joon ;
KSBB Journal, volume 25, issue 5, 2010, Pages 409~420
Biopolymer-based materials recently have garnered considerable interest as they can decrease dependency on fossil fuel. Biopolymers are naturally obtainable macromolecules including polysaccharides, polyphenols, polyesters, polyamides, and proteins, that play an important role in biomedical applications such as tissue engineering, regenerative medicine, drug-delivery systems, and biosensors, because of their inherent biocompatibility and biodegradability. However, the insolubility of unmodified biopolymers in most organic solvents has limited the applications of biopolymer-based materials and composites. Ionic liquids (ILs) are good solvents for polar organic, nonpolar organic, inorganic and polymeric compounds. Biopolymers such as cellulose, chitin/chitiosan, silk, and DNA can be fabricated from ILs into films, membranes, fibers, spheres, and molded shapes. Various biopolymer/biopolymer and biopolymer/synthetic polymer composites also can be prepared by co-dissolution of polymers into IL mixtures. Heparin/biopolymer composites are especially of interest in preparing materials with enhanced blood compatibility.
Role of Plant Extracts to Remove Hydrogen Sulfide in the Air-Lifter Reactor
Park, Jong-Woo ; Park, Young-G. ; Kim, Jeong-In ;
KSBB Journal, volume 25, issue 5, 2010, Pages 421~428
This paper reports on the experimental investigation carried out to evaluate the physical optimal conditions in the absorption column to remove odorous hydrogen sulfide gas. Hydrogen sulfide gas, as a highly undesirable contaminant, is most widely emitted from environmental treatment facilities. The absorbent mixed with natural second metabolites extracted from conifer trees and chemical absorbent of 2-aminoethanol was applied to remove it via chemical neutralization. The absorbent of natural second metabolites was achieved by a removal efficiency of 20-40% by itself depending on the treatment conditions, but the complex absorbent mixed with 0.1% amine chemical provides the removal efficiency of 98%. The optimal removal efficiencies have been examined against the two major parameters of temperature and pH. This study shows that the aqueous solution by natural second metabolites can be used as an appropriate absorbent in the column absorbed for the removal of hydrogen sulfide gas.
Isolation of Mutant Strains from Keratinase Producing Bacillus subtilis SMMJ-2 and Comparision of Their Enzymatic Properties
Ko, Hee-Sun ; Kim, Hyun-Soo ;
KSBB Journal, volume 25, issue 5, 2010, Pages 429~436
Keratinase is widely used in certain industrial applications. The present study sought to improve the culture conditions of Bacillus subtilis SMMJ-2 to facilitate mass production of keratinase. Strain SMMJ-2 was irradiated by ultraviolet light and the resulting isolates were tested for keratinase activity. Isolates displaying elevated keratinase activity were selected and used to determine the optimum temperature (24, 30, 37, 45,
) for bacterial keratinase production during a 4 day incubation period. The highest enzyme activity (55 units/mL/min), from a Bacillus subtilis SMMJ-2 mutant (mutant No. 2) was demonstrated following incubation at
. The effects of carbon and nitrogen sources on keratinase production were confirmed by measuring the enzyme activity from the culture broth of the mutant strain cultured in various media containing different carbon source and nitrogen sources during a 4 day period. The optimal medium composition for producing keratinase consisted of 1% glucose, 0.7%
, and 1.2% soybean meal. Optimal initial pH and temperature for producing keratinase were 7.0 and
, respectively. Keratinases produced by B. subtilis SMMJ-2 and the mutant No. 2 were purified from the culture broth which used soybean meal as a nitrogen source. Membrane ultrafiltration, DEAE-sephacel ion exchange and Sephadex G-100 gel chromatography were used to purify the enzymes. The purified keratinases from both B. subtilis SMMJ-2 and the mutant No. 2 showed single bands and their molecular weights were estimated as 28 kDa and 42 kDa, respectively on SDS-polyacrylamide gel electrophoresis.
Ultrasound-Assisted Extraction of Canola Oil Using Supercritical Fluid Process
Hwang, Ah-Reum ; Lim, Gio-Bin ; Ryu, Jong-Hoon ;
KSBB Journal, volume 25, issue 5, 2010, Pages 437~442
The objective of this study was to investigate the effect of ultrasound on the extraction of oil from canola seeds when supercritical carbon dioxide (
) was used as an extraction solvent. The ultrasound-assisted
extraction were carried out while varying such operating parameters as particle size of crushed canola seed, flow rate of
, aspect ratio of the extraction vessel, and ultrasound power. The extraction rate decreased with increasing particle size of samples, showing a maximun at a
flow rate of 6.2 L/min. Both the extraction rate and extraction yield increased with a decrease in the aspect ratio of the extraction vessel. For the ultrasoundassisted
extraction, the extraction yield was slightly increased when the
flow rate was below 6 mL/min with sample A and B.
Preparation of Coated Citric Acid for Sensory Improvement of Chocolate Products
Kim, Bok-Hee ; Kim, Dong-Man ; Lee, Sang-Hwa ; Shin, Hyun-Jae ;
KSBB Journal, volume 25, issue 5, 2010, Pages 443~448
Coated citric acid as an acidulant was prepared to be used as a stable food additive. Sensory evaluation of chocolate products with the coated acidulant additive was performed to improve sensory preference tendency in chocolate product. When the ratio of coated acidulant was varied on the preparation of dark (0, 1, 2 wt%), milk (0, 1, 2 wt%) and white (0, 2, 4, 6 wt%) chocolate, 1 wt% (dark and milk) or 2 wt% (white) addition showed better preference than that of 0% sample in overall acceptability. Under the condition of the same coated acidulant content (1% or 2%), the white chocolate showed best sensory evaluation result in sweet, sour taste and overall acceptability.
Monitoring of the Optical Purity for Levothyroxine Sodium in Pharmaceuticals Using Crown Ether Derived Chiral Columns
Jeon, So-Hee ; Lee, Won-Jae ;
KSBB Journal, volume 25, issue 5, 2010, Pages 449~452
L-Thyroxine possessing a chiral center, the naturally occurring thyroid hormone has been used for the treatment of thyroid dysfunctions and marketed as levothyroxine (L-thyroxine) sodium salt. In this study, after extraction of levothyroxine tablet as a pre-treatment process, direct enantiomer separation of thyroxine on crown ether derived chiral columns for determination of optical purity was performed using reversed mobile phase with acid additive. The chromatographic method developed in this study was applied in the determination of optical purity of several current domestic and foreign commercialized levothyroxine tablets. Optical purity values of these commercialized L-thyroxine sodium tablets except one were higher than 99 percents.
Development of Nanoenzymes for the Production of Glucose from Seaweed and Various Polysaccharide
Jin, Lie-Hua ; Lee, Jung-Heon ;
KSBB Journal, volume 25, issue 5, 2010, Pages 453~458
The magnetically separable polyaniline nanofiber enzymes were developed for the recycle of enzyme and enhanced enzyme stability. The stability of enzyme was maintained over 90% for 8 days under room temperature and vigorous shaking conditions (200 rpm). The residual activity of immobilized enzyme was over 60% after 8 days incubation at
. Glucose was produced from various polysaccharides, agarose, curdlan, cellulose, and sea weed, using magnetically separable immobilized enzyme. Glucose production rate with curdlan was 1.2 g/(l h) and showed high decomposition rate due to high mass transfer. After 10 times recycle, the residual activity of immobilized enzyme was over 75%. 1 g/L of glucose was produced with 5 mg of immobilized enzymes.
Simultaneous Determination of Tar Color Additives in Cold Syrups by Ion Pair-high Performance Liquid Chromatography
Jin, Jing-Yu ; Huang, Hu ; Lee, Beom-Gyu ; Lee, Won-Jae ;
KSBB Journal, volume 25, issue 5, 2010, Pages 459~465
A simple and efficient analytical method for the simultaneous determination of seven tar color additives was developed using ion pair high performance liquid chromatography. The conditions for HPLC analysis were as follows: column,
-Bondapak C18 (10
3.9 mm i.d.); gradient mobile phase, 0.025 mol/L ammonium acetate (containing 0.01 mol/L tetrabutylammonium bromide)-acetonitrile-methanol (65:25:10) as a mobile for fraction A and 0.025 mol/L ammonium acetate (containing 0.01 mol/L tetrabutylammonium bromide)-acetonitrilemethanol (40:50:10) as a mobile for fraction B; flow rate, 1.0 mL/ min; detection wavelength, 254/520/620 nm. We could attain to the detection limits as 0.01~0.05
g/mL (254 nm) and 0.005~0.01
g/mL (520 nm) for six red tar color additives, and 0.05
g/mL (254 nm) and 0.002
g/mL (620 nm) for Fast green FCF. This analytical method was applicable to determine the tar color additives contained in several commercial cold syrups.
Use of Selective Ethanol Adsorption for Ethanol Concentration
Jin, Li-Hua ; Lee, Jung-Heon ;
KSBB Journal, volume 25, issue 5, 2010, Pages 466~470
In this study, we developed simple process for ethanol concentration. We developed magnetically separable polyanilline nanofiber (PAMP) for selective ethanol adsorption. PAMP can adsorbed 80% of ethanol in the solution. After adsorption, the ethanol was recovered with simple magnetic separation and centrifugation process. After 10 times recycle of PAMP, the ethanol adsorption maintained 92% of its initial adsorption capacity. Using ethanol concentration process, the ethanol concentration increased up to 197.6 g/L from 46 g/L which was 4.3 folds increase.
Comparison of Bio-ethanol Productivity Using Food Wastes by Various Culture Modes
Kang, Hee-Jeong ; Li, Hong-Xian ; Kim, Yong-Jin ; Kim, Seong-Jun ;
KSBB Journal, volume 25, issue 5, 2010, Pages 471~477
In order to improve bio-ethanol productivity by various cultivation methods in this paper, the culture modes using food wastes, such as batch culture, high-cell-density fermentation, SSF (simultaneous saccharification and fermentation) by fill & draw, continuous culture by fill & draw were performed and their productivities were compared. SSFs by fill & draw were performed by continuous decompression using 1 L evaporator system, and by 10 L bioreactor without decompression. In addition, the continuous cultures by fill & draw mode using SFW (saccharafied food wastes) medium were performed by changes of 40% culture broth with intervals of 12 h (0.03
), 6 h (0.07
), 3 h (0.13
). Consequently, productivities of bio-ethanol were 2.52 g/L-h and 1.30 g/L-h in batch culture and high- cell-density fermentation, respectively. The productivities of SSF by fill & draw showed 2.24 g/L-h and 2.03 g/L-h in continuous decompression with 1 L evaporator and 10 L bioreactor without decompression, respectively. Also, the productivities in continuous culture by fill & draw modes showed 2.02 g/L-h, 4.07 g/L-h and 6.25 g/L-h by medium change with intervals of 12 h, 6 h, and 3 h, respectively. In conclusion, the highest ethanol productivity was obtained in the continuous culture mode by fill & draw with dilution rate of 0.13
Inhibitory Melanogenesis of Bambusae caulis in Taeniam and Profiling of Related Proteins
Lee, Chung-Hyun ; Kim, Sang-Bum ; Byun, Sang-Yo ;
KSBB Journal, volume 25, issue 5, 2010, Pages 478~482
Inhibitory melanogenesis by Bambusae caulis in Teaniam (Phyllostaachys nigra var. henonis Stapf) was studied. Tyrosinase inhibition activities were evaluated with six different extracts. Among them the extract with methanol showed the highest tyrosinase activity inhibition. MTT assay with B16 melanoma showed that the extract was not toxic up to the concentration of 50 ppm. The melanogenesis was clearly inhibited by the extract when it was examined by the melanin content assay in the cell. When the extract was dosed as 10 ppm, the melanogenesis was reduced to 68% in culture medium and 74% in the cell. By the proteome analysis with 2-D electrophoresis, 171 protein spots were found in the control gel and 282 spots were detected in the sample gel. Among 120 spot proteins matched, 12 spots were identified as proteins involved in the melanogenesis mechanism.
Production of Biodiesel from High Acid Value Oils using Amberlyst-15
Sim, Yeon-Ju ; Kim, Eui-Yong ;
KSBB Journal, volume 25, issue 5, 2010, Pages 483~489
Biodiesel has attracted great attention as an alternative renewable energy source for the replacement of petroleumbased diesel fuel, yet its high production cost due to expensive oil feedstock remainsas the major economical obstacle. In this study, we investigated catalysts and reaction conditions for the acid catalyzed pre-conversion of free fatty acid (FFA) to fatty acid methyl ester (FAME) in cheap low-grade oils of high acid value. The NaOH base catalyzed reaction of vegetable oil of the initial acid value of 2 mg KOH/g led to a high FAME conversion above 95.4%, but the conversion abruptly decreased at higher initial acid values. This base catalyzed reaction was practically ineffective displaying the FAME conversion below 15% even at the initial acid value of 10 mg KOH/g by the severe saponification side reaction. Among the various catalysts studied for the pre-conversion of FFA to FAME, Amberlyst-15 was the most effective in reducing the acid value, and the optimum reaction condition identified was
with oil to methanol ratio of 1:3 and catalyst concentration of 15% (w/w). As the results, great enhancements in the overall biodiesel conversion were achievable via a consecutive reaction of the acid catalyzed FFA pre-conversion to FAME under the optimal condition obtained with Amberlyst-15 followed by the NaOH base catalyzed reaction, far above the extent which was obtainable by the single NaOH catalyzed reaction.
Prediction of ORFs in Metagenome by Using Cis-acting Transcriptional and Translational Factors
Cheong, Dea-Eun ; Kim, Geun-Joong ;
KSBB Journal, volume 25, issue 5, 2010, Pages 490~496
As sequencing technologies are steadily improving, massive sequence data have been accumulated in public databases. Thereby, programs based on various algorithms are developed to mine useful information, such as genes, operons and regulatory factors,from these sequences. However, despite its usefulness in a wide range of applications, comprehensive analyses of metagenome using these programs have some drawbacks, thereby yielding inaccurate or complex results. We here provide a possibility of signature sequences (cis-acting transcriptional and translational factors of metagenome) as a hallmark of ORFs finding from metagenome.