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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 26, Issue 6 - Dec 2011
Volume 26, Issue 5 - Oct 2011
Volume 26, Issue 4 - Aug 2011
Volume 26, Issue 3 - Jun 2011
Volume 26, Issue 2 - Apr 2011
Volume 26, Issue 1 - Feb 2011
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Biodegradation of Diesel by Rhodococcus fascians in Sand Column
Moon, Jun-Hyung ; Koo, Ja-Ryong ; Yun, Hyun-Shik ;
KSBB Journal, volume 26, issue 1, 2011, Pages 1~6
DOI : 10.7841/ksbbj.2011.26.1.001
Contamination of soils, groundwater, air and marine environment with hazardous and toxic chemicals is major side effect by the industrialization. Bioremediation, the application of microorganism or microbial processes to degrade environmental contaminant, is one of the new environmental technologies. Because of low water solubility and volatility of diesel, bioremediation is more efficient than physical and chemical methods. The purpose of this study is biodegradation of diesel in sand by using Rhodococcus fascians, a microorganism isolated from petroleum contaminated soil. This study was performed in the column containing sand obtained from sea sides. Changes in biodegradability of diesel with various flow rates, inoculum sizes, diesel concentrations, and pH were investigated in sand column. The optimal condition for biodegradation of diesel by R. fascians in sand column system was initial pH 8 and air flow rate of 30 mL/min. Higher diesel degradation was achieved at larger inoculum size and the diesel degradation by R. fascians was not inhibited by diesel concentration up to 5%.
Acute Toxicity Assessment of New Algicide, Thiazolidinedione Derivative (TD53) to Marine Ecosystem
Yim, Eun-Chae ; Shin, Jun-Jae ; Park, In-Taek ; Han, Hyo-Kyung ; Kim, Si-Wouk ; Cho, Hoon ; Kim, Seong-Jun ;
KSBB Journal, volume 26, issue 1, 2011, Pages 7~12
DOI : 10.7841/ksbbj.2011.26.1.007
In order to perform an acute toxicity assessment of a new algicide, thiazolidinedione derivative (TD53) with enhanced solubility and lower toxicity to marine ecosystem, representative 3 organisms: plant plankton (Skeletonema costatum), animal plankton (Daphnia magna), fish (Paralichthys olivaceus) related in the food chain of marine ecosystem according to OECD standard methods were employed in the exposure experiment. The exposure assessment showed that
of S. costatum in 96-hour,
of D. magna in 48-hour and
of P. olivaceus in 72-hour for TD53 were
respectively. NOEC (No Observed Effect Concentration) and PNEC (Predicted No Effect Concentration) were calculated to be
and 6.10 nM, respectively from
of most sensitive strain, D. magna. Comparing with the results of toxicity assessment previously performed by using Ulva pertusa Kjellman accepted as an ISO standard method, the values of PNEC showed 3.7 times lower toxicity in case of this study employing 3 organisms, indicating that if the organisms which are more representative and sensitive in marine ecosystem are further investigated, more accurately and validly predicted toxicity of TD53 could be applied in field.
Optimization of Precipitation Process for the Recovery of Lactic Acid
Choi, Kook-Hwa ; Chang, Yong-Keun ; Kim, Jin-Hyun ;
KSBB Journal, volume 26, issue 1, 2011, Pages 13~18
DOI : 10.7841/ksbbj.2011.26.1.013
In this study, precipitation process was developed for the recovery of the lactic acid from calcium lactate fermentation broth. Calcium lactate yield was improved by decreasing the solubility of calcium lactate through the addition of ethanol (25%, v/v) as a co-precipitant. The optimal lime type, lime concentration, stirrer speed, precipitation time, temperature, and solvent amount for
precipitation were CaO, 0.0175 g/mL, 220 rpm, 24 h,
, ethanol 25% (v/v), respectively. Lactic acid was easily and efficiently recovered from precipitated
by adding sulfuric acid (
molar ratio=1:1). In the model solution of organic acids and fermentation broth, the overall yields of recovered lactic acid were 62% and 55%, respectively, under the aforementioned optimal conditions.
Isolation and Antibacterial Activity of Actinomycetes Producing Growth Inhibition Compounds Against Multi-antibiotic Resistant Pseudomonas Aeruginosa
Kang, Dong-Hee ; Bae, Ho-Kyung ; Kim, Hyun-Soo ;
KSBB Journal, volume 26, issue 1, 2011, Pages 19~26
DOI : 10.7841/ksbbj.2011.26.1.019
Of the 500 Actinomycetes isolates obtained from soil, one isolate grown on maltose as the sole carbon source produced compound BHK-P19, which inhibited the growth of multiple drug resistant P. aeruginosa 0245. Ultraviolet radiation mutagenesis curtailed production of BHK-P19. Mutation of the BHK-P19 producer using N-methyl-N'-nitro-N-nitroso-guanidine obviated the antibacterial activity to P. aeruginosa 0245, but not towards P. aeruginosa 0225. The mixing of BHK-P19 and BHK-S5 culture extracts inhibited P. aeruginosa 0254, 0225 and 1113. The combined application of BHK-P19 culture extract and Schizandra chinensis Baillon extract inhibited P. aeruginosa 0254, 0225, 0826, 1113, 1378, 1731 and 2492. Use of various concentrations of BHK-P19 culture extract and ampicillin markedly increased antibacterial activity against multi-drug resistant P. aeruginose 1113.
Antioxidant Effect of HotWaterExtract of Muraenesox cinereus's Skin
Shin, Hyun-Jae ; Kim, Yoon-Soo ; Nam, Hyung-Gun ; Na, Myung-Soon ; Kim, Mi-Hye ; Kang, Hyeong-Bong ; Lee, Hee-Duck ; Choi, Du-Bok ; Choi, On-You ; Cha, Wol-Suk ;
KSBB Journal, volume 26, issue 1, 2011, Pages 27~32
DOI : 10.7841/ksbbj.2011.26.1.027
For the development of functional food and cosmetics using hot water extract of Muraenesox cinereus's skin, contents of vitamin, amino acid and element, and antioxidant activity were investigated. The results are shown as follows: among vitamins, A(0.21mg/100 g), C (78.12mg/100 g),
(0.03 mg/100 g), E (1.97 mg/100 g) and Niacin (2.53mg/100 g) were detected, respectively.Mineral contents were an order of K > P > Na > Mg > Ca > Fe and Zn. Contents of total amino acids were an order of Pro > Gly > Arg > Glu > Phe > Ala. Especially, the sum of total amino acids was 27.17 mg/100 mL, which was about 4.0 fold higher than that of free amino acid. DPPH radical scavenging activity of hot water extract of M. cinereus's skin at 25 mg/mL was 63.5% and did not increase at above 50 mg/mL. Activities of antioxidant enzymes in the liver of ethanol-treated rats using hot water extract of M. cinereus's skin were investigated. Compared to control group, activities of ADH and GSH-px were decreased. In the case of CAT and SOD activity, they were increased. These results showed that the hot water extract of M. cinereus's skin can be applied to raw macterial for functional food and cosmetics.
Anti-diabetic Effect of the Exopolysaccharides (EPS) Produced from Cordyceps sinensis on ob/ob Mice
Choi, Jang-Won ;
KSBB Journal, volume 26, issue 1, 2011, Pages 33~40
DOI : 10.7841/ksbbj.2011.26.1.033
Anti-diabetic effect of the exopolysaccharides (EPS) produced from submerged mycelial culture of Cordyceps sinensis (Cs) was studiedin a type II diabetic animal model (C57BL/6J ob/ob). This study was designed to determine whether Cs-EPS improves clinical symptoms of type II diabetes in ob/ob mice. After Cs-EPS treatment at doses of 200 mg/kg body weight, the fasting blood glucose levels decreased by 47% after 7 weeks compared with those of the control mice. According to the oral glucose tolerance test, the glucose levels recovered its baseline after 120 min in Cs-EPS-treated mice, although the blood glucose levels increased significantly after 30 min. On the other hand, the control group (not-treated) did not recovered its initial level of glucose after 120 min. Furthermore, food intake, body weight, total plasma cholesterol and triglyceride concentrations in ob/ob mice treated with Cs-EPS were significantly decreased, compared with those in control ob/ob mice. Cs-EPS treatment increased significantly the plasma insulin level and the expression of leptin mRNA in adipose tissue of Cs-EPS-treated ob/ob mice. From these results, it is demonstrated that Cs-EPS could be effective for regulating normal blood glucose levels by increasing the amounts of plasma insulin and leptin expression in ob/ob mice, indicating that this compound could be a candidate material as a dietary supplement to control hyperglycemia in patients suffering from type II diabetes.
Enhancement of Cyclo-His-Pro (CHP) Content from Soybean Fermented with Bacillus amyloliquefaciens CHP-12 and Its Anti-diabetic Effect
Ra, Kyung-Soo ; Choi, Jang-Won ;
KSBB Journal, volume 26, issue 1, 2011, Pages 41~48
DOI : 10.7841/ksbbj.2011.26.1.041
To enhance cyclo-His-Pro (CHP) content, soybean hydrolysate was obtained using the strains isolated from Chungkukjang and further purified by various purification steps. First, twenty two strains were screened from Chungkukjang containing high level of CHP. Among them, the strain No. 12, which showed higher productivity of CHP from soybean ferment and have homologous sequence with 16S rDNA of Bacillus amyloliquefaciens, was named B. amyloliquefaciens CHP-12. Through various purification processes, CHP was concentrated from soybean ferment using ultrafiltration, which showed the best efficiency of CHP production, with the yield (71.3%) and CHP content (2.14 mg/g). Moreover, when glucose tolerance test was performed in Type I Sprague-Dawley rat induced by streptozotocin using the soybean ferments [0.5 g soybean ferment/kg body weight (CHP-0.5 group)] and 1.0 g soybean ferment/kg body weight (CHP-1.0 group), there were significant differences in glucose levels between diabetes-control group (265.3 mg/dL) and soybean ferment-treated groups (CHP-0.5 group: 84.3 mg/dL and CHP-1.0 group: 85.3 mg/dL) 120 min after glucose injection (2 g/kg body weight) (p < 0.05). Accordingly, it is suggested that the soybean ferment containing high level of CHP might be a candidate material as an anti-diabetic supplement for manufacturing functional healthy foods.
Process Development for Deordorization of Fucoidan Using a Combined Method of Solvent Extraction and Spray Drying
Cho, Eun-Hye ; Park, Keun-Hyoung ; Kim, So-Young ; Oh, Chang-Seok ; Bang, Soon-Il ; Chae, Hee-Jeong ;
KSBB Journal, volume 26, issue 1, 2011, Pages 49~56
DOI : 10.7841/ksbbj.2011.26.1.049
Volatile components of crude fucoidan and its raw material, Undaria pinnatifida sporophylls were identified by GC/MSD analysis, and the effects of a combined method of solvent extraction and drying (hot-air drying or spray drying) on deordorization of fucoidans were examined. The major components of seaweed smell (odor) in crude fucoidan and Undaria pinnatifida sporophylls were identified as alkanes (octadecane, heptadecane, tetradecane) and acids (hexadecanoic acid, oleic acid). Solvent extraction and drying were combined for the deordorization of fucoidans: hot-air drying/deordorization process (HDDP) and spray drying/deordorization process (SDDP). The deordorization effects of solvent extraction using solvents including water, ethanol, and acetone were investigated. Among the tested solvents, ethanol was selected as a deordorization solvent, because the seaweed smell and taste were mostly lowered by ethanol extraction followed by drying. In addition, HDDP and SDDP were compared, showing that SDDP gave higher sensory characteristics than HDDP. When the crude fucoidan was treated by ethanol extraction for 30 min followed by SDDP, approximately 60% of off-flavor components of crude fucoidan was removed based on GC/MSD peak area, and the total sugar and sulfate contents and APTT-anticoagulant activity significantly increased compared to crude fucoidan. In conclusion, SDDP after ethanol treatment was proposed and established as a deordorization process for fucoidan preparation.
Optimized Production of Biosurfactant by the Indigenous Bacterium, Pseudoalteromonas sp. HK-3 Originating from Oil-Spilled Areas
Cho, Su-Hee ; Ma, Chae-Woo ; Oh, Kye-Heon ;
KSBB Journal, volume 26, issue 1, 2011, Pages 57~61
DOI : 10.7841/ksbbj.2011.26.1.057
The principal objective of this study was to determine the optimal conditions for the production of biosurfactant by the indigenous bacterium, Pseudoalteromonas sp. HK-3, originating from oil-spilled areas. The relationship between total biosurfactant production and the factors affecting biosurfactant production were evaluated by statistical analysis using SPSS software. The effects of various supplemental carbon sources (e.g., glucose, dextrose, mannitol, citrate, acetate) on the maximal production of biosurfactant by the test culture of Pseudoalteromonas sp. HK-3 was then evaluated. As a result, mannitol was found in this study to be the best supplemental carbon source for the production of biosurfactant. A spot inoculation of crude cultural liquid containing the HK-3 cells generated the largest clear zone, whereas only small clear zones appeared around the spots inoculated with either supernatant only or cell pellets following centrifugation. Our results demonstrated that the HK-3 test culture supplemented with 2% mannitol at an initial pH of 6 generated the maximal amount of biosurfactant within 72 h of incubation.
The Effect of Lactobacillus plantarumCLP-1 on the Swine Viruses
Lee, Gun-Hee ; Kim, Young-Hee ; Cho, Hyeon-A ; Kang, Sung-Gi ; Kim, Dong-Gun ;
KSBB Journal, volume 26, issue 1, 2011, Pages 62~68
DOI : 10.7841/ksbbj.2011.26.1.062
To isolate Lactic acid bacteria for animals, we have screened from Kim-chi, swine intestine, swine feces, and dairy products by random selection and anti-viral, antipathogenic bacteria test. Among them, CLP-1 shown that inhibitory effect against rotavirus, porcine epidemic diarrhea (PED) virus, Salmonella sp, and E.coli. By examining biological property, API-ZYM and identified Lactobacillus plantarum by 16S rDNAgene sequence. CLP-1 determined resistance to low pH and bile salt. Futhermore, the cell body of CLP-1 adhered to the intestinal epithelium tissue of swine and Caco-2 cell. CLP-1 was examined on cell immune system modulating activity in vitro. The whole cell and cell culture supernatant was increasing of interferon-
activity. And then, CLP-1 increased prevention effect by Salmonella enteritidis infection in SPF chickens. And we determined similar result in pigs.
Production of Gemcitabine-Loaded Poly (L-lactic acid) Microparticles Using Supercritical Carbon Dioxide: Effect of Process Parameters
Joo, Hyun-Jae ; Jung, In-Il ; Lim, Gio-Bin ; Ryu, Jong-Hoon ;
KSBB Journal, volume 26, issue 1, 2011, Pages 69~77
DOI : 10.7841/ksbbj.2011.26.1.069
In this study, poly (L-lactic acid) (PLLA) microparticles containing gemcitabine hydrochloride were prepared by a supercritical fluid process, called aerosol solvent extraction system (ASES), utilizing supercritical carbon dioxide as antisolvent. The influence of process parameters such as temperature, pressure,
and solution flow rate, solution concentration, and feed ratio of drug to polymer on the morphology and characteristics of the microparticles was studied in detail. The gemcitabine-loaded microparticles exhibited a spherical shape with a smooth surface. The entrapment efficiency of gemcitabine increased with increasing temperature, solution concentration and
flow rate and with decreasing drug/polymer feed ratio. The maximum drug loading obtained from the ASES process was found to be about 11%. The ASES-processed PLLA microparticles containing gemcitabine showed a relatively high initial burst due to the presence of surface pores on the microparticles and the poor affinity between drug and polymer.
Effect of Silybin on Body Weight and Glucose Tolerance in High-fat-diet Induced Obese Mice
Hur, Haeng-Jeon ; Hwang, Jin-Taek ;
KSBB Journal, volume 26, issue 1, 2011, Pages 78~82
DOI : 10.7841/ksbbj.2011.26.1.078
In this study, we investigated the effects of silybin on body weight and glucose tolerance in mice fed high fat diet mice. We found that body weight, plasma TG contents, fat size, glycerol, 3-hydroxybutyrate and total cholesterol were significantly decreased in silybin (500 mg/kg) supplemented groups compared to high fat diet group. Whereas, total food intake was not changed between high fat diet group and high fat diet plus silybin group. Futhermore, supplement of high fat elevated the glucose intolerance and was improved in silybin supplement group. Finally, we examined the effect of silybin on circulating adipocytokine level to explore the possible mechanism by which silybin improves high fat diet-induced obesity and diabetes. The silybin supplement significantly reduced the level of adipocytokine, such as leptin, resistin, IL-6, and MCP-1 induced by high fat diet. These results suggest that silybin can be used to improve obesity and diabetes.
Stability Evaluation of the Cosmetics Containing Lotus Leaf Extract
Choi, Sun-Ju ; Kim, So-Young ; Jeong, Yoon-Joo ; Ku, Chang-Sub ; Ha, Buung-Jhip ; Chae, Hee-Jeong ;
KSBB Journal, volume 26, issue 1, 2011, Pages 83~86
DOI : 10.7841/ksbbj.2011.26.1.083
The stability of cream containing lotus leaf extract (LLE) was evaluated. Stability pH and viscosity were measured at 3 different temperatures (
), and at room temperature under the sun light at 2 day intervals during 12 days. The cleansing cream and foam cleansing containing the extract did not show a significant pH change at the different temperatures. However, the pH of the cleansing cream and foam cleansing containing LLE slightly decreased at
. They showed a high stability at temperature conditions (
) and under the sun light condition. The cleansing cream and foam cleansing containing LLE did not show a significant viscosity change at
, but had a tendency of decrease at
. There was no significant offflavor and discoloration as well as physical changes such as creaming and cohesion at
and under the sun light condition. And this LLE could be used as a stable functional cosmetic material.
Automatic Counting of Yeast Cells in Baker's Yeast Culture Using PC Camera and Conventional Light Microscope
Lee, Hyeong-Choon ;
KSBB Journal, volume 26, issue 1, 2011, Pages 87~91
DOI : 10.7841/ksbbj.2011.26.1.087
Automatic counting of yeast cells in baker's yeast culture was tried using a conventional light microscope equipped with a pc camera. Relatively good binary image was obtained by using white LED as microscope light source, but uneven brightness distribution in original image hindered counting accuracy. A block binarization method using local thresholds proportional to local brightnesses was used to get improved binary images. The brightnesses of the blocks were expressed as the value component in HSV color model. Good quality binary images were obtained by binarization on
blocks of original images and connected-component labelling of the binarized images produced reliable counting results in the concentration range