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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 29, Issue 6 - Dec 2014
Volume 29, Issue 5 - Oct 2014
Volume 29, Issue 4 - Aug 2014
Volume 29, Issue 3 - Jun 2014
Volume 29, Issue 2 - Apr 2014
Volume 29, Issue 1 - Feb 2014
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Production and Characterization of Antifungal Chitinase of Bacillus licheniformis Isolated from Yellow Loess
Han, Gui Hwan ; Bong, Ki Moon ; Kim, Jong Min ; Kim, Pyoung Il ; Kim, Si Wouk ;
KSBB Journal, volume 29, issue 3, 2014, Pages 131~138
DOI : 10.7841/ksbbj.2014.29.3.131
In this study, we isolated two novel chitinase producing bacterial strains from yellow loess samples collected from Jullanamdo province. The chitinase producing bacteria were isolated based on the zone size of clearance in the chitin agar plates. Both of them were gram positive, rod (
), spore-forming, and motility positive. They were facultative anaerobic, catalase positive and hydrolyzed starch, gelatin, and casein. From the 16s rRNA gene sequence analysis, the isolates were labeled as Bacillus licheniformis KYLS-CU01 and B. licheniformis KYLS-CU02. The isolates showed higher extracellular chitinase activities than B. licheniformis ATCC 14580 as a control. The optimum temperature and pH for chitinase production were
and pH 7.0, respectively. Response Surface Methodology (RSM) was used to optimize the culture medium for efficient production of the chitinase. Under this optimal condition, 1.5 times higher chitinase activity of B. licheniformis KYLS-CU02 was obtained. Extracellular chitinases of the two isolates were purified through ammonium sulfate precipitation and anion-exchange DEAE-cellulose column chromatography. The specific activities of purified chitinase from B. licheniformis KYLS-CU01 and B. licheniformis KYLS-CU02 were 7.65 and 5.21 U/mg protein, respectively. The molecular weights of the two purified chitinases were 59 kDa. Further, the purified chitinase of B. licheniformis KYLS-CU01 showed high antifungal activity against Fusarium sp.. In conclusion, these two bacterial isolates can be used as a biopesticide to control pathogenic fungi.
Removal of Cupper(II), Zinc(II) in Marine Environment by Heavy Metal Resistant Desulfovibrio desulfuricans
Joo, Jeong Ock ; Kim, In Hwa ; Oh, Byung-Keun ;
KSBB Journal, volume 29, issue 3, 2014, Pages 139~144
DOI : 10.7841/ksbbj.2014.29.3.139
Microorganisms play a significant role in bioremediation of heavy metal contaminated seawater. In this study, we reported an effective removal of Cu and Zn in marine envionment by using Desulfovibrio desulfuricans (D. desulfuricans) which belong to sulfate reducing bacteria. D. desulfuricans showed stable growth characteristics in high salt concentration and had resistance to heavy metals. Cu and Zn was removed not only by physical adsorption on the surface of bacteria but also by precipitation reaction of microbial metabolism by D. desulfuricans in seawater. In case of different heavy metal concentration, Cu was effectively removed 85% at 25 ppm and 60% at 50 ppm and Zn was effectively removed 54% at 50 ppm and 46% at 200 ppm, respectively.
Quality Changes of Fresh Vegetable and Fruit Juice by Various Juicers
Choi, Moon-Hee ; Kim, Min-Joo ; Jeon, Young-Jin ; Shin, Hyun-Jae ;
KSBB Journal, volume 29, issue 3, 2014, Pages 145~154
DOI : 10.7841/ksbbj.2014.29.3.145
A fresh vegetable and fruit juice has become a new healthy food available for detoxification, dieting and health. This paper presents the useful information about the quality changes of fresh juice according to different juicer. Quality of fresh juice could be evaluated by several factors such as juice yield, enzyme activity, antioxidant activity, polyphenol contents, and anti-inflammatory activity. The juice yields of 12 different vegetables and fruits were compared using 6 different juicers and it was observed that the yield of slow juicer was better than that of conventional blender. Among 12 samples, the juice yield of grape is the best and the pH of the juice was in the acidic range of 3 and 4. Kiwi and grapefruit were the best in terms of protease enzyme activities by Hemoglobin units on the tyrosine basis and Spectrophotometric acid protease unit and papain units on the tyrosine basis of KFDA protocols. The total polyphenol contents were also high in kiwi and grapefruit. The antioxidant activity by diphenyl-1-picrylhydrazyl (DPPH), superoxide dismutase (SOD), and radical scavenging assay were high in the order of kiwi, grapefruit, grape, tomato, and orange. Anti-inflammatory activities were also assay for 12 samples with 6 juicers. It can be concluded that of fresh fruit and vegetable juice provides a source of antioxidant components and enzymes with high activity. And the enzyme activities could be used as one of the quality indicator of fresh juice. Concerning the juicers used in this study, slow juicer could be recommended to prepare the fresh juice in terms of the juice quality.
Enhanced Production of Succinic Acid by Actinobacillus succinogenes using the Production Medium Supplemented with Recombinant Carbonic Anhydrases
Park, Sang-Min ; Eum, Kyuri ; Kim, Sangyong ; Jeong, Yong-Seob ; Lee, Dohoon ; Chun, Gie-Taek ;
KSBB Journal, volume 29, issue 3, 2014, Pages 155~164
DOI : 10.7841/ksbbj.2014.29.3.155
Succinic acid, a representative biomass-derived platform chemical, is a major fermentation product of Actinobacillus succinogenes. It is well known that carbon dioxide is consumed during the succinate fermentation, but the biochemical mechanism behind this phenomenon is not yet understood well. In this study, it was found that the addition of carbonic anhydrase (CA)s into media significantly enhances the succinic acid production by A. succinogenes during the fermentation supplied with carbon dioxide. It is likely that the (bi) carbonate produced by the CA activity from gaseous carbon dioxide is favoured by A. succinogenes for consumption and utilization. Therefore, the
requirement could be significantly reduced without compromising the succinate productivity. Furthermore, because of too high price of the commercial carbonic anhydrase, it was undertaken to economically overproduce a cyanobacterial carbonic anhydrase by the use of a recombinant Pichia pastoris. An expression vector system was constructed with the carbonic anhydrase gene PCR-cloned from Cyanobacterium Synechocystis sp., and introduced into P. pastoris for fermentation studies. About 95.9 g/L of succinic acid was produced in the production medium with 30 ppm of carbonic anhydrase, approximately 2 fold higher productivity compared to the parallel process with no supplementation of the enzyme. It is expected that this method can provide a valuable way of overcoming inefficiencies inherent in gas supply during
-based bioprocesses like succinic acid fermentation.
Statistical Optimization of Production Medium for Enhanced Production of Succinic Acid Produced by Anaerobic Fermentations of Actinobacillus succinogenes
Park, Sang-Min ; Chun, Gie-Taek ;
KSBB Journal, volume 29, issue 3, 2014, Pages 165~178
DOI : 10.7841/ksbbj.2014.29.3.165
Statistical medium optimization has been carried out for the production of succinic acid in anaerobic fermentations of Actinobacillus succinogenes. Succinic acid utilized as a precursor of many industrially important chemicals is a fourcarbon dicarboxylic acid, biosynthesized as one of the fermentation products of anaerobic metabolism by A. succinogenes. Through OFAT (one factor at a time) experiments, corn steep liquor (CSL), a very cheap agricultural byproduct, was found to have significant effects on enhanced production of succinic acid, when supplemented along with yeast extract. Hence, using these factors including glucose as a carbon/energy source, interactive effects were investigated through
full factorial design (FFD) experiments, showing that the concentration of each component (i.e., glucose, yeast extract and CSL) should be higher. Further statistical experiments were conducted along the steepest ascent path, followed by response surface method (RSM) in order to find out optimal concentrations of each constituent. Consequently, optimized concentrations of glucose, yeast extract and CSL were observed to be 180 g/L, 15.08 g/L and 20.75 g/L respectively (10 g/L of
and 100 g/L of
to be supplemented as bicarbonate suppliers), with the estimated production level of succinic acid to be 92.9 g/L (about 3.5 fold higher productivity as compared to the initial medium). Notably, the RSM-estimated production level was almost similar to the amount of succinic acid (92.9 g/L vs. 89.1 g/L) produced through the actual fermentation process performed using the statistically optimized production medium.
Antimicrobial Activity of Essential Oil of Pinus koraiensis Seed Against Pathogens Related to Acne
Choi, Jae-Wan ; Kim, Ran ;
KSBB Journal, volume 29, issue 3, 2014, Pages 179~182
DOI : 10.7841/ksbbj.2014.29.3.179
The purpose of the present research was to evaluate the antimicrobial activity of the essential oil extracted from Pinus koraiensis seed against pathogens related to acne. The essential oil was extracted by steam distillation method. The chemical compositions of essential oil were analyzed by GC-MS. Alpha-pinene (29.87%), D-limonene (19.26%), betapinene (11.19%), beta-myrcene (3.84%), n-hexadecanoi acid (3.2%), beta-caryphyllene (2.72%), and cyclohexene (2.17%) were main components. This essential oil had antimicrobial activities against Malasseizia furfur, Propionibacterium acnes, and Staphylococcus epidermidis.
Development of Formaldehyde-shielding Chitosan-gel Sheet
Kim, So-Yeon ; Kim, Mi-So ; Tak, Sang Min ; Lee, Ji Whan ; Sim, So Yeon ; Joo, Eun Hee ; Kim, Sung Bae ; Kim, Chang-Joon ;
KSBB Journal, volume 29, issue 3, 2014, Pages 183~187
DOI : 10.7841/ksbbj.2014.29.3.183
Sick-building syndrome occurs when indoor air is polluted with harmful volatile organic compounds such as formaldehyde which are contained in furniture or new building materials. In this study, formaldehyde-shielding chitosangel sheet was developed and its performance was evaluated. Chitosan and agar were dissolved in acetic acid solution. The optimal concentrations of chitosan, acetic acid and agar were 3, 3, and 2.5 %(w/w). Formaldehyde was spreaded on gypsum board and then wall paper was attached on it by using glue. When chitosan-gel sheet was attached on this control board, the amount of formaldehyde released from the board was decreased by 63% than in control board. On the other hand, decrease in formaldehyde releasing was only 32% when liquid solution of chitosan was spreaded on the control board. This result clearly indicates that chitosan-gel sheet removes formaldehyde more effectively than liqud solution of chitosan. Furthermore, this type of sheet is more applicable to new building than spraying type.
Sporulation of Lysinibacillus sphaericus WJ-8 Isolated from Concrete Pavement and Response to Environmental Stresses
Han, Sang-Hyun ; Kang, Chang-Ho ; Shin, Yu Jin ; Yeom, Woo Sung ; Jeong, Jin Hoon ; So, Jae-Seong ;
KSBB Journal, volume 29, issue 3, 2014, Pages 188~192
DOI : 10.7841/ksbbj.2014.29.3.188
Calcite forming bacteria (CFB) have been received increasing attention as a novel and environmental friendly strategy for the healing of concrete crack. Among the CFB, spore forming bacteria were proposed to overcome concrete condition (high pH, hydration heat, deicer). In this study, Lysinibaclillus sphaericus WJ-8 (WJ-8) isolated from concrete pavement was characterized. The WJ-8 was able to precipitate calcite at 10 mg/mL. When observed by scanning electron microscopy, WJ-8 showed spore formation and maximum spore yield was approximately 97.9%. Also response of spores against various environment stresses was examined. Approximately 83~97% of spores maintained their survivability at each three conditions (
, 3 M NaCl and pH 12).
Pretreatment of Wastepaper using Aqueous Glycerol under High Pressure to Enhance Enzymatic Hydrolysis
Seo, Dong Il ; Kim, Chang-Joon ; Kim, Sung Bae ;
KSBB Journal, volume 29, issue 3, 2014, Pages 193~198
DOI : 10.7841/ksbbj.2014.29.3.193
Pretreatment of wastepaper using aqueous glycerol under high pressure was studied to enhance the enzymatic hydrolysis. The pretreatment was conducted over a wide range of conditions including temperatures of
, sulfuric acid concentrations of 0.5~1.5%, and reaction times of 30~90 minutes. After the effect of glycerol concentration on the pretreatment performance was investigated, 70% glycerol was selected. As glycerol concentration was increased, higher digestibility was achieved due to higher lignin removal. The optimum condition was found to be around
, 1%, and 60 minutes. At this condition, 60% and 35% of hemicellulose and lignin, respectively, were removed, while only 5% of cellulose was lost. The enzymatic digestibility was 76%, meaning that 73% of the glucan present in the initial substrate was recovered as glucose after enzymatic hydrolysis. Also, it was found that the temperature and acid concentration than the reaction time were more strongly related to the compositional removals and enzymatic digestibility.
Effect of Anti-oxidant Activity and the Skin Whitening Action on Plantago asiatica L. Root Extract
Yoon, Mi Yun ; Han, Young Sook ;
KSBB Journal, volume 29, issue 3, 2014, Pages 199~204
DOI : 10.7841/ksbbj.2014.29.3.199
To investigate the effect of Plantago asiatica L. Root extract on skin care, we measured anti-oxidant activity and whitening action. As a result of measuring DPPH radical scavenging activity to examine independent anti-oxidation of PRE, there was slight scavenging activity. Fluorescent materials DHE, DCF-DA and DHR were each used to measure superoxide anion, hydrogen peroxide, and hydroperoxide created in RAW 264.7 cells, all concentrations were found to dependently prevent ROS production. Tyrosinase activation was found to be blocked dose-dependant. Melanin production was also prevented dose-dependant, but the effects were slight. Therefore, it is expected to be used effectively in development of functional cosmetic materials.
Effect of Mobile Phase Additive on Enantiomer Resolution for Chiral Amines on Polysaccharide-derived Chiral Stationary Phases by High Performance Liquid Chromatography
Paik, Man-Jeong ; Yoon, Hye-Ran ; Lee, Wonjae ;
KSBB Journal, volume 29, issue 3, 2014, Pages 205~209
DOI : 10.7841/ksbbj.2014.29.3.205
Chromatographic enantiomer resolution of chiral amines was performed on several covalently immobilized and coated chiral stationary phases (CSPs) based on polysaccharide derivatives under the mobile phase conditions containing base or acid or acid/base additive. The chromatographic parameters including separation factors and capacity factors were greatly influenced by the nature of the mobile phase containing base or acid or salt additive as well as the used CSPs. When 0.05% triethylamine/0.05% trifluoroacetic acid as an additive in the mobile phase was used on all CSPs in this study, the greatest enantiomer resolution was observed except for Chiralpak AD. Also, it was shown that the change of base additive into acid or salt in the mobile phase may directly affect chiral recognition mechanisms between the chiral selectors and analytes occurring during enantiomer separation, resulting in the change of elution orders.
Antibacterial Activities of Fermented Sayuksan Ingredient Extracts for Multidrug-resistant Strains
Park, Young-Ja ; Kang, Dong Hee ; Kim, Hyun-Soo ;
KSBB Journal, volume 29, issue 3, 2014, Pages 210~219
DOI : 10.7841/ksbbj.2014.29.3.210
Sayuksan has been widely applied to treat a variety of diseases such as acute hepatitis, gastritis, and colitis. Sayuksan consists of medicinal herbs such as Glycrrhizae uralensis Fischer, Paeonia lactiflora Pallas, Bupleurum falcatum Linne, and Poncirus trifoliata Rafinesqul. Methanol extracts (1 mg/mL) from the four kinds of medicinal herbs did not show antibiotic activities against general test strains and multi-drug resistant strains. The antibacterial activity of fermented medicinal herbs extracts with Lactobacillus spp. strain was confirmed as Gram-positive bacteria which are higher than Gram-negative bacteria. Extracts of Glycrrhizae uralensis Fischer fermented with Lb. casei KCTC 3109 displayed inhibitory diameters of 16 mm against Pseudomonas aeruginosa P01828. Superoxide dismutase (SOD)-like activity of the medicinal herb extracts was not determined, but the extract of Paeonia lactiflora Pallas fermented with six strains of Lactobacillus spp. had the highest antioxidant activity. SOD-like activity of Paeonia lactiflora Pallas extracts fermented by Lb. brevis KCTC 3498 was
, which was the highest antioxidant activity among the fermented extracts with the other medicinal herbs.
Optimization of Spirogyra Flocculation Using Polyaluminium Chloride
Baek, Jaewon ; Choi, Jong-Il ;
KSBB Journal, volume 29, issue 3, 2014, Pages 220~224
DOI : 10.7841/ksbbj.2014.29.3.220
Flocculation is known one of the effective methods for harvesting microalgae. This study was aimed to optimize the flocculation condition for decreasing the amounts of flocculant and obtaining the highest yield of algal biomass. To achieve this goal, it was optimized the flocculant concentration, reaction pH and the concentration of cell density for harvest using response surface methodology (RSM). The flocculation of microalgae, Spirogyra varians, was carried out using inorganic flocculant polyaluminium chloride. By the RSM result, the optimal flocculation condition was calculated 5 ppm of polyaluminum chloride, pH 7.5 and 0.33 of optical cell density at
. The obtained recovery yield of S. varians was 97.6% at the optimal condition.