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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 29, Issue 6 - Dec 2014
Volume 29, Issue 5 - Oct 2014
Volume 29, Issue 4 - Aug 2014
Volume 29, Issue 3 - Jun 2014
Volume 29, Issue 2 - Apr 2014
Volume 29, Issue 1 - Feb 2014
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Production of Polyhydroxybutyrate from Crude Glycerol and Spent Coffee Grounds Extract by Bacillus cereus Isolated from Sewage Treatment Plant
Lee, Gi Na ; Choi, So Young ; Na, Jonguk ; Youn, HaJin ; Jang, Yu-Sin ;
KSBB Journal, volume 29, issue 6, 2014, Pages 399~404
DOI : 10.7841/ksbbj.2014.29.6.399
Production of biodegradable polymer polyhydroxyalkanoates (PHAs) from industrial wastes exhibits several advantages such as recycle of waste and the production of high valuable products. To this end, this study aimed at isolating from the sewage treatment plant a PHA producing bacterium capable of utilizing wastes generated from biodiesel and food industries. A Bacillus cereus strain capable of producing poly(3-hydroxybutyrate) [P(3HB)] was isolated, which was followed by confirmation of P(3HB) accumulation by gas-chromatographic analyses. Then, the effects of nutrient limitation on P(3HB) production by B. cereus was first examined. Cells cultured in a minimal medium under the limitation of nitrogen, potassium and sulfur suggested that nitrogen limitation allows the highest P(3HB) accumulation. Next, production of P(3HB) was examined from both waste of biodiesel production (crude glycerol) and waste from food industry (spent coffee grounds). Cells cultured in nitrogen-limited minimal medium supplemented crude glycerol and waste spent coffee grounds extract accumulated P(3HB) to the contents of 2.4% and 1.0% of DCW. This is the first report demonstrating the capability of B. cereus to produce P(3HB) from waste raw materials such as crude glycerol and spent coffee grounds.
Preparation of Gemcitabine-Loaded PLLA/Gemcitabine-PLLA Microparticles Using Supercritical Fluid and Their Release Characteristics
Hong, Jin-Hee ; Lim, Giobin ; Ryu, Jong-Hoon ;
KSBB Journal, volume 29, issue 6, 2014, Pages 405~413
DOI : 10.7841/ksbbj.2014.29.6.405
In this study, gemcitabine (Gem)-Poly(L-lactic acid) (PLLA) conjugates were synthesized through an amide linkage reaction. Then, the microparticles of Gem-PLLA/PLLA blends containing gemcitabine were prepared using a supercritical fluid process, called aerosol solvent extraction system (ASES). Gemcitabine-loaded Gem-PLLA/PLLA microparticles obtained from the ASES process showed a spherical shape. The amount of gemcitabine released after 30 day incubation in a phosphate buffer solution of pH 7.4 was about 90% of the total amount of gemcitabine present in the product.
Ethanol Production from Red, Brown and Green Seaweeds and Biosorption of Heavy Metals by Waste Seaweed Slurry from Ethanol Production
Sunwoo, InYung ; Ra, ChaeHun ; Kwon, SeongJin ; Heo, JiHee ; Kim, Ye-Jin ; Kim, JiWoo ; Shin, JiHo ; Ahn, En-Ju ; Cho, YuKyeong ; Kim, Sung-Koo ;
KSBB Journal, volume 29, issue 6, 2014, Pages 414~420
DOI : 10.7841/ksbbj.2014.29.6.414
The seaweeds with high carbohydrate ratio Gelidium amansii, Saccharina japonica and Enteromorpha intestinalis were used as red, brown, and green seaweeds, respectively. Thermal acid hydrolysis, enzymatic saccharification and fermentation were carried out using those seaweeds to produce ethanol. The ethanol concentrations from red, brown and green seaweed were 14.8 g/L, 11.6 g/L and 9.9 g/L, respectively. After the production of ethanol, the seaweeds were reused to absorb heavy metal. The maximum biosorption ratio was Cu(II) (89.6%), Cr(III) (82.9%), Ni(II) (66.1%). Cu(II) had the highest affinity with 3 waste seaweeds. Red seaweed was verified the most effective substrates to both process.
Antioxidant Activities of Extracts of Solanum nigrum L.
Kim, A-Ram ; Kim, Dong-Hyun ; Pyeun, Soo Min ; Jeong, Gangwon ; Gwon, Jae Hwan ; Yeo, Chung Won ; Lee, Jung-Seag ; Jeong, Gwi-Taek ;
KSBB Journal, volume 29, issue 6, 2014, Pages 421~425
DOI : 10.7841/ksbbj.2014.29.6.421
In this study, the antioxidant activities of fractions (methanol, hexane, methylene chloride, butanol, ethyl acetate and water) of Solanum nigrum L. extract was investigated. The contents of total phenolic compounds of each fractions of methanol, butanol, methylene chloride, ethyl acetate, hexane and water are
, respectively. For assay of antioxidant activity, 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity, reducing power and nitrite scavenging activity are evaluated. In DPPH radical scavenging activity, the highest effect was obtained from the fraction of ethyl acetate. Reducing power is ordered as ethyl acetate > methylene chloride > methanol. In nitrite scavenging activity, the highest activity was 5.5% (butanol fraction), whereas hexane fraction did not detected. Overall, antioxidant activities are closely related the content of phenolic compound in extracts of S. nigrum L.
Effects and Optimization of Gamma-Amino Butyric Acid (GABA) Production Process using Glutamate Decarboxylase (GAD)
Kim, Eui Jin ; Lee, Jung-Heon ;
KSBB Journal, volume 29, issue 6, 2014, Pages 426~431
DOI : 10.7841/ksbbj.2014.29.6.426
pH controlled batch reactor and bubble column reactors have been developed in this research. They were used to produce high concentration of GABA and to determine optimal pH for GABA production. Glutamate decarboxylase (GAD) was isolated from recombinant E. coli and used for GABA production from monosodium glutamate (MSG). pH control was inevitable because the pH increased with MSG consumption. GAD showed highest activity at acidic conditions at pH 5.5 but the optimal pH for GABA production was pH 6.0. When 1.5 mole of MSG was used as reactant, the 1.05 mole of GABA was produced after 10 hrs batch reaction. Using bubble column reactors, 80 % of MSG was converted to GABA for 6 hrs reaction and 1.2 mole of GABA was produced.
Antioxidative Activity of Extract of Cornus walteri Wanger Leaves in Human Dermal Fibroblast Irradiated by UVB
Park, Hyun-Chul ; Jung, Taek Kyu ; Yoon, Kyung-Sup ;
KSBB Journal, volume 29, issue 6, 2014, Pages 432~436
DOI : 10.7841/ksbbj.2014.29.6.432
We investigated antioxidative activity of the ethanol extracts of leaves of Cornus walteri Wanger (CWE) by treated enzyme in human dermal fibroblast (HDFs) irradiated by UVB. We examined the in vitro chemical and cellular antioxidant activities of CWE in HDFs. We employed scavenging assay for the 1,1-diphenyl-2,5-picrylhydrazyl (DPPH) radicals and cellular antioxidative activity of CWE, and we was investigated in
-treated or UVB-irradiated HDFs. The CWE effectively scavenged DPPH radicals (
) when compared to the scavenging activities of L-ascorbic acid (
). CWE reduced UVB-induced cellular damage in HS68 cells by MTT assay and inhibited intracellular ROS generation in dose-dependent manner. In addition, CWE also attenuated the elevated levels of 8-isoprostane resulting from UVB-mediated oxidative stress. Collectively, these results suggest that CWE could be a new potential candidate as antioxidant against UVB-induced oxidative stress in HDFs.
Biological Activity of Aloe Vera Gel and Skin Extracts
Cho, Eunhye ; Kim, Soyoung ; Bang, Soonil ; Kim, Dong Chung ; In, Man-Jin ; Chae, Hee Jeong ;
KSBB Journal, volume 29, issue 6, 2014, Pages 437~442
DOI : 10.7841/ksbbj.2014.29.6.437
In vitro biological activities of Aloe vera gel and skin extracts were evaluated. Total polyphenol contents of Aloe vera skin were measured 41.12 mg/g. DPPH radical scavenging activity of Aloe vera skin-70% EtOH extract, Aloe vera skin-water extract, Aloe vera gel-70% EtOH extract and Aloe vera gel-water extract were 55%, 38%, 11% and 10%, respectively. In addition, 70% EtOH extract and water extract were compared with respect to SOD-like antioxidant activity of Aloe vera-70% EtOH extract has higher activity than Aloe vera water extract. Tyrosinase inhibition rate of Aloe vera gel extract was higher than Aloe vera skin extract. Alcohol dehydrogenase (ADH) and Acetaldehyde dehydrogenase (ALDH) relative percentage activity of Aloe vera gel extract were 126% and 216%, respectively. It was suggested that Aloe vera gel and skin extracts could be used as a functional biomaterial for functional food and cosmetics.
Biosynthesis of Lactate-containing Polyhydroxyalkanoates in Recombinant Escherichia coli from Sucrose
Oh, Young Hoon ; Kang, Kyoung-Hee ; Shin, Jihoon ; Song, Bong Keun ; Lee, Seung Hwan ; Lee, Sang Yup ; Park, Si Jae ;
KSBB Journal, volume 29, issue 6, 2014, Pages 443~447
DOI : 10.7841/ksbbj.2014.29.6.443
Biosynthesis of lactate-containing polyhydroxyalkanoates (PHAs) was examined in recombinant Escherichia coli W strain from sucrose. The Pseudomonas sp. MBEL 6-19 phaC1437 gene and the Clostridium propionicum pct540 gene, which encode engineered Pseudomonas sp. MBEL 6-19 PHA synthase 1 (
) and engineered C. propionicum propionyl-CoA transferase (
), respectively, were expressed in E. coli W to construct key metabolic pathway to produce poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)]. The recombinant E. coli W expressing the phaC1437 gene and the pct540 gene could synthesize P(3HB-co-13mol%LA) up to the polymer content of 31.3 wt% when it was cultured in chemically defined MR medium containing 20 g/L of sucrose and 2 g/L of sodium 3-hydroxybutyrate. When Ralstonia eutropha phaAB genes were additionally expressed to provide 3-hydroxybutyrate-CoA (3HB-CoA) from sucrose, P(3HB-co-16mol%LA) could be synthesized from sucrose as a sole carbon source without supplement of sodium 3-hydroxybutyrate in culture medium, but the PHA content was decreased to 12.2 wt%. The molecular weight of P(3HB-co-16 mol%LA) synthesized in E. coli W using sucrose as carbon source were
), respectively, which are not different from those that have previously been reported by other recombinant E. coli strains. Engineered E. coli strains developed in this study should be useful for the production of lactate-containing PHAs from sucrose, one of the most abundant and least expensive carbon sources.