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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 30, Issue 6 - Dec 2015
Volume 30, Issue 5 - Oct 2015
Volume 30, Issue 4 - Aug 2015
Volume 30, Issue 3 - Jun 2015
Volume 30, Issue 2 - Apr 2015
Volume 30, Issue 1 - Feb 2015
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Optimum Reaction Condition of Enzymatic Hydrolysis for Production of Reducing Sugar from Enteromorpha intestinalis
Kim, A-Ram ; Kim, Dong-Hyun ; Jeong, Gwi-Taek ;
KSBB Journal, volume 30, issue 2, 2015, Pages 53~57
DOI : 10.7841/ksbbj.2015.30.2.53
In this study, the production of total reducing sugar from macro green-algae Enteromorpha intestinalis by enzymatic hydrolysis was investigated. As a result of enzymatic hydrolysis using 13 kind commercial enzymes, the highest yield of 8.75% was obtained from Viscozyme L, which is multi-enzyme complex such as cellulase, arabanase, beta-glucanase, hemicellulase and xylanase. As a control, only 0.33% and 0.27% yield were obtained from 1% sulfuric acid and 0.05 M citrate buffer (pH 4.8), respectively. In the case of enzyme mixture, the mixture of
CTec2 (1:1) was presented the highest yield of 10.67%. Finally, the 14.99% yield was obtained at 36 hr under the condition of 10% biomass and 30% enzyme mixture.
Efficacy of Supplemental Lecithin-free Egg Yolk at Different Dietary Calcium Levels on Growth Performance and Ca Absorption of Laying Hens
Han, Jong-Kwon ; Oh, Mihyang ; Nam, Jungok ; Ji, Kibbeum ; Sim, Insuk ; Park, Keun-Tae ;
KSBB Journal, volume 30, issue 2, 2015, Pages 58~62
DOI : 10.7841/ksbbj.2015.30.2.58
A 3-weeks feeding trial with 288 laying hens were conducted to determine the efficacy of lecithin-free egg yolk at different levels of dietary Ca on performance and Ca absorption. Laying hens were divided into 6 groups according to calcium level and testing agent; 0% calcium feed (A), 0.2% calcium feed (B), 0.4% calcium feed (C, normal feed), 0.6% calcium feed (D), 0.4% calcium feed + 0.2% egg byproduct (C+0.2), 0.4% calcium feed + 0.4% egg byproduct (C+0.4). The final body weight gain of C+0.2 and C+0.4 groups were higher by 1.5% and 7.4% respectively than group C. Tibia ash contents did not show significantly difference, but calcium contents increase (p<0.05) in C+0.2 and C+0.4 groups. Parallel undecalcified tibia joint sections were stained for calcium absorption by the von Kossa's stain. This result show that lecithin free egg byproduct supplementation to normal calcium feed improved growth performance and calcium utilization in laying hens.
Optimization of Demucilage Process of Opuntia ficus-indica var. saboten Fruit using High Hydrostatic Pressure Enzyme Dissolution
Im, Sungbin ; Lee, Hyungjae ; Shim, Jae-Yong ; Kim, Tae-Rahk ; Kim, Dae-Ok ;
KSBB Journal, volume 30, issue 2, 2015, Pages 63~68
DOI : 10.7841/ksbbj.2015.30.2.63
This study aimed to develop and optimize a demucilaging process of Opuntia ficus-indica var. saboten (OFI) fruit to increase its usability as functional food ingredient and food additive. Viscozyme and Novozym 33095 as multienzyme complex having a broad spectrum of carbohydrases and pectolytic enzymes, respectively, were used in enzymatic dissolution along with high hydrostatic pressure liquefaction. To optimize the liquefaction process using high hydrostatic pressure liquefying extractor, response surface methodology with 3-factor central composite design was employed with reaction factors such as temperatures (25, 32, 40, 48, and
), pressures (20, 40, 60, 80, and 100 MPa), and times (15, 30, 45, 60, and 75 min). At optimum conditions (
, 100 MPa, and 58.275 min) for high hydrostatic pressure liquefaction process, the processed OFI fruit juice was predicted to have viscosity at 2.917 poise, partly due to the release of free sugars such as fructose and glucose detected using HPLC-ELSA system. The results above suggests that the OFI fruit juice with decreased viscosity may be used for various manufacturing processes of food, beverage, ice cream, and cosmetics.
Study of Effectiveness of Antimicrobial on Restraining Formation of Biofilms on the Surface of Aluminum
Park, SangJun ; Oh, YoungHwan ; Jo, BoYeon ; Choi, MiYeon ; Hyun, MinWoo ; Jeong, JaeHyun ; Kim, EuiYong ;
KSBB Journal, volume 30, issue 2, 2015, Pages 69~76
DOI : 10.7841/ksbbj.2015.30.2.69
The antibacterial activity of a antimicrobial (organic synthetic or organic natural material) on the bacteria (Bacillus megaterium, Arthrobacter oxydans, Micrococcus luteus, Methylobacterium aquaticum) detected in the automobiles showed 99.9% bacteria decrease rate within 30 min of being in contact with the tested bacteria culture. The MIC of the organic synthetic material based antimicrobials and the organic natural material based antimicrobial on the bacteria were 31~500 mg/mL and 8~250 mg/mL, respectively. The bacteria and biofilms were formed on the surface of aluminum after 5 ~8 days in the case of addition of the organic synthetic material based antimicrobial to the MIC values for the tested bacteria culture. On the other hand, there was no proliferation of bacteria and formation of biofilms on the surface of aluminum even after 30 days in the case of addition of the organic natural material based antimicrobial to the MIC values for the tested bacteria culture. As a result, the organic natural material based antimicrobial was confirmed to be more excellent effect of inhibition of bacterial proliferation and restraint of biofilms formation than the organic synthetic material based antimicrobial.
Antioxidative and Anti-inflammatory Activities of Carrot flower
Kim, Su-Gyeong ; Byun, Hoo-Dhon ; Kim, Sang Cheol ; Yang, Kyong-wol ; Kim, Jeong Hee ; Han, Jong-Heon ;
KSBB Journal, volume 30, issue 2, 2015, Pages 77~81
DOI : 10.7841/ksbbj.2015.30.2.77
The antioxidant and anti-inflammatory activities of extract and its fraction of Daucus carota var. sativa flower were studied in vitro. Extract and ethyl acetate fraction, butanol fraction of carrot flower showed radical scavenging effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH). We also investigated the effect of extract and ethyl acetate fraction, butanol fraction of carrot flower on NO production in a lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells. Extract and its fraction of carrot flower significantly inhibited NO production and this inhibitory action was not due to the cytotoxicity. This study suggests that extract and ethyl acetate fraction, butanol fraction of Daucus carota var. sativa flower could contribute to the chemoprevention and therapy of oxidative stress and inflammation.
Enhanced Production of hGM-CSF by Immobilized Transgenic Plant Cell Cultures
Noha, Yun-Sook ; Nama, Hyung-Jin ; Choi, Hong-Yeol ; Tak, Sa-Ra ; Kim, Dong-Il ;
KSBB Journal, volume 30, issue 2, 2015, Pages 82~90
DOI : 10.7841/ksbbj.2015.30.2.82
Plant cell immobilization can protect plant cells from shear forces and increase the stability of gene. An additional advantage of immobilization is the easiness for performing continuous culture with cell recycling. Therefore plant cell immobilization can overcome the limitations of plant cell applications. In addition, target protein should be selected from pharmaceutical proteins to get rid of low expression level problem. The enhanced production of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) was investigated in immobilized Nicotiana tabacum suspension cell cultures. When the cells were immobilized in polyurethane foam, specific production of hGM-CSF was higher than that in alginate bead immobilization. Optimum continuous culture condition was the addition of 60 g/L sucrose in growth media with exchanging media every 6 day. Under the same condition, specific hGM-CSF production was 7 times higher in a 500-mL spinner flask than that in 100-mL Erlenmeyer flasks. Therefore, development of an effective immobilization process would be possible when the advantage of easy cell recycling was used. Consequently, enhanced production of target proteins could be possible in immobilized continuous cultures when the advantages of immobilization were applied.
Effect of Nutrient Limitation on Lipid Content and Fatty Acid Composition of Mutant Chlamydomonas reinhardtii
Baek, Jaewon ; Choi, Jong-il ;
KSBB Journal, volume 30, issue 2, 2015, Pages 91~95
DOI : 10.7841/ksbbj.2015.30.2.91
Production of biodiesel from microalgae is dependent on the microalgal lipid content and free fatty acid composition. Both lipid and free fatty acid are regulated by nutrient sources. In this study, newly developed mutant Chlamydomonas reinhardtii with higher lipid content was investigated for the effect of nutrient limitation. Nitrogen
were limited for nutrient starvation during the cultivation. Under nutrient starvation, total lipid content level was increased to 27~33% and C16:0 fatty acid content constituted over 31~43% of total fatty acid. Interestingly, we also found that the expression of fatty acid desaturase (FAD7) was decreased when nutrients were starved.