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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 30, Issue 6 - Dec 2015
Volume 30, Issue 5 - Oct 2015
Volume 30, Issue 4 - Aug 2015
Volume 30, Issue 3 - Jun 2015
Volume 30, Issue 2 - Apr 2015
Volume 30, Issue 1 - Feb 2015
Selecting the target year
A Study on the Effect of the Rhizobacterium, Bacillus sp. SH1RP8 and Potassium Family Polymers on the Crop Growth under Saline
Hong, Sun Hwa ; Kim, Ji Seul ; Park, Jang Woo ; Lee, Eun Young ;
KSBB Journal, volume 30, issue 3, 2015, Pages 97~102
DOI : 10.7841/ksbbj.2015.30.3.97
This study aimed to evaluate the potential plantgrowth promoting effects of potassium polyacrylate, a superabsorbent polymer, and Bacillus sp. SH1RP8, a family of plant-growth-promoting bacteria. Potassium polyacrylate was selected as the polymer for use due to its high molecular weight and its ability to retain and continuously supply moisture. Plant-growth-promoting rhizobacteria (PGPR) were isolated from the soil and applied to plants growing in dry environments, such as saline conditions. The moisture absorption and retention abilities of potassium polyacrylate were evaluated at a high temperature (
) and in a dry condition, during which time the polymer showed a water retention potential of 19606.07% after 29 days. To overcome the reaming problem in the soil environment, natural polymers (such as cellulose) were mixed with the potassium acrylate. The shoot growths of Peucedanum japonicum Thunb and Arundo donax were significantly enhanced when treated with the mixture of the isolated rhizosphere bacterium SH1RP8 and potassium polyacrylate (63.5 and 124.3%, respectively).
In situ Recovery of hGM-CSF in Transgenic Rice Cell Suspension Cultures
Myoung, Hyun-Jong ; Choi, Hong-Yeol ; Nam, Hyung-Jin ; Kim, Dong-Il ;
KSBB Journal, volume 30, issue 3, 2015, Pages 103~108
DOI : 10.7841/ksbbj.2015.30.3.103
Production of foreign proteins by transgenic plant cell cultures has several advantages such as post-translational modification, low risk of product contamination and low-cost production and purification. However, target proteins are degraded by extracellular proteases existing in the media. A solution to this problem is the use of perfusion culture and ion exchange chromatography for the application of integrated bioprocess using in situ recovery. With this method, production of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) was investigated in this study. First, optimization of cell concentration during the induction phase for the production of hGM-CSF was examined. As cell concentration increased, the level of hGM-CSF was decreased due to the presence of extracellular proteases. Induction using sugarfree media produced 33% more hGM-CSF. The effects of pH on the binding of hGM-CSF to cationic and anionic exchange resins were also investigated. In terms of stability, optimal pH was found to be 5~7. In the case of using buffer exchange when CM-Sepharose was used as a cationic exchange resin, optimal pH for binding was 4.8 and adsorption yield was 77%. When DEAE-Sepharose was used as an anionic exchange resin, it was 5.5 (74%). Without buffer exchange, optimal pH was 4.6 (84%). From these results, an integrated bioprocess using in situ recovery with simultaneous production and separation of foreign protein in transgenic plant cell suspension cultures was found to be feasible.
Analysis of Ingredient Mixtures for Cryoprotection and Gastrointestinal Stability of Probiotics
Jeong, Eun Ji ; Moon, Dae Won ; Oh, Joon Suk ; Moon, Jin Seok ; Kim, Kwang Yup ; Choi, Hye Sun ; Han, Nam Soo ;
KSBB Journal, volume 30, issue 3, 2015, Pages 109~113
DOI : 10.7841/ksbbj.2015.30.3.109
Current drying and encapsulation methods for probiotics manufacturing are complicate and cost-burdened processes. The aim of this study was to develop a simple ingredient mixture to make probiotic granules via one-step process, providing not only a cryoprotective effect during freezing and drying but also high survival ratio in gastrointestinal tract. As cryoprotectans, commercially available ingredients including skim milk, monosaccharide (trehalose or glycerin), maltodextrins (with low or high degree of equivalents) were used. Their cryoprotective effect during lyophilization and survival ratios in artificial gastric juice and bile salt were measured against 3 strains of lactic acid bacteria (LAB) (Lactobacillus plantarum, Lb. brevis, and Lactococcus lactis). As results, 3 mixtures with different compositions showed a cryprotective effect on LAB tested and the best compostion was dependant upon LAB; skim milk 10%, trehalose 15%, glycerin 0.5%, and NaCl 1% was for Lb. plantarum and Lc. lactis, and maltodextrin 10% instead of skim milk was for Lb. brevis. In addition, those mixtures showed similar survival effect on LAB tested. These results demonstrate that skim milk or maltodextrins with trehalose, glycerin, and NACl can be effectively used for onestep lyophilization of LAB as an alternative method of encapsulation.
Development of Microbe Carrier for Bioremediation of Zn, As by using Desulfovibrio Desulfuricans and Zeolite in Artificial Sea Water
Kim, In Hwa ; Choi, Jin-Ha ; Joo, Jeong Ock ; Oh, Byung-Keun ;
KSBB Journal, volume 30, issue 3, 2015, Pages 114~118
DOI : 10.7841/ksbbj.2015.30.3.114
In this study, we have developed a microbe-carrier that combined Desulfovibrio desulfuricans and zeolite for removal of Zn and As in contaminated seawater. Desulfovibrio desulfuricans, one of the sulfate-reducing bacteria (SRB) microorganism was exhibited stable growth characteristics in highly salted water and strong resistance to Zn and As contaminated seawater. Moreover, zeolites are one of the most useful carrier to remove heavy metals from wastewaters. The results showed that SRB immobilized zeolite carrier can enhance removal ratio of Zn and As. In addition, heavy metals tended to be better removed in medium at conditions of
. In case of heavy metal concentration, they were effectively removed ranging from 50 to 100 ppm. These results show that SRB-zeolite carriers hold great potential to remove cationic heavy metal species from industrial wastewater in marine environment.
Characteristic of Arthrospira platensis Enhanced Antioxidant Activity
Choi, Soo-Jeong ; Lee, Jae-Hwa ;
KSBB Journal, volume 30, issue 3, 2015, Pages 119~124
DOI : 10.7841/ksbbj.2015.30.3.119
Arthrospira platensis (A. platensis) is a microscopic and filamentous cyanobacterium that derives its name from the spiral or helical nature of its filaments. In this study, we induced mutants of A. platensis through NMU treatment and selected two strains by level of ipid contents. We named mutant '1-9', '2-5', and they were cultivated in the same way with the wild type. During 12 days cultivation, cell growth, dry cell weight, pigment content, and lipid content were measured for characteristics of mutants. As a result, pigment and lipid content of mutants were increased about 3.6, 1.8 times compared with wild type, respectively. It was shown that total flavonoid and polyphenol contents of mutants were increased about 1.5 times compared with wild type. And radical scavenging effect of mutants were increased about 10% compared with wild type.
The Application of Thermotolerant Yeast Kluyveromyces marxianus as a Potential Industrial Workhorse for Biofuel Production
Park, Jae-Bum ; Kim, Jin-Seong ; Jang, Seung-Won ; Hong, Eunsoo ; Ha, Suk-Jin ;
KSBB Journal, volume 30, issue 3, 2015, Pages 125~131
DOI : 10.7841/ksbbj.2015.30.3.125
Kluyveromyces marxianus is a well-known thermotolerant yeast. Although Saccharomyces cerevisiae is the most commonly used yeast species for ethanol production, the thermotolerant K. marxianus is more suitable for simultaneous saccharification and fermentation (SSF) processes. This is because enzymatic saccharification usually requires a higher temperature than that needed for the optimum growth of S. cerevisiae. In this study, we compared the fermentation patterns of S. cerevisiae and K. marxianus under various temperatures of fermentation. The results show that at a fermentation temperature of
, K. marxianus exhibited more than two fold higher growth rate and ethanol production rate in comparison to S. cerevisiae. For SSF using starch or corn stover as the sole carbon source by K. marxianus, the high temperature (
) fermentations showed higher enzymatic activities and ethanol production compared to SSF at
. These results demonstrate the potential of the thermotolerant yeast K. marxianus for SSF in the industrial production of biofuels.
Stabilization and Antifungal Activity of Isolated Symbiotic Bacteria from Entomopathogenic Nematodes
Kang, Dong-Hee ; Kim, Hyo-Hyun ; Nam, Uk-Ho ; Kim, Hyun-Soo ;
KSBB Journal, volume 30, issue 3, 2015, Pages 132~139
DOI : 10.7841/ksbbj.2015.30.3.132
In order to use the symbiotic bacteria from ethomophatogenic nematodes as a biological control agent for agriculture, the cultural condition for maintaining phase I and antifungal activity was investigated. Symbiotic bacteria (SB) 1 stain from nematodes were selected from the three strains isolated from entomopathogenic nematodes. The growth of the SB 1 strain in NB, TSB, TY and YS medium was higher than that of the SB 2 and SB 3 strain. The packed cell volume of the SB 1 strain was reduced in NB medium which showed radical pH change. Phase I of the SB 1 strain was maintained in TSB medium after being stored for 2 weeks at
. Culture broth with the SB 1 strain in TSB medium for 6 days and 7 days showed antifungal activities against Rhizoctonia solani KACC 40142, Botrytis cinerea Pers. KACC 40854, and Botrytis cinerea Pers. KACC 41008. Culture broth with the SB 1 strain in TSB medium containing 100 mM L-proline for 5 days showed antifungal activities against Rhizoctonia solani KACC 40142, and Botrytis cinerea Pers. KACC 40854.