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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 30, Issue 6 - Dec 2015
Volume 30, Issue 5 - Oct 2015
Volume 30, Issue 4 - Aug 2015
Volume 30, Issue 3 - Jun 2015
Volume 30, Issue 2 - Apr 2015
Volume 30, Issue 1 - Feb 2015
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Biodecolorization and Biodegradation of Dye by Fungi: A Review
Cho, Kyung-Suk ; Ryu, Hee Wook ;
KSBB Journal, volume 30, issue 5, 2015, Pages 203~222
DOI : 10.7841/ksbbj.2015.30.5.203
In recent years, there has been an intensive research on the application of degradative activities of fungi for treatment of various non-degradable materials such as petroleum hydrocarbons, polychlorinated biphenyls, pesticides, polycyclic aromatic hydrocarbons, dyes and so on. Chief of all, the fungal treatment technology is received the spotlight as one of the most promising alternatives to replace present methods for the treatment of dye wastewater. The present paper reviews the recent trend in research on the decolorization and biodegradation of dyes by various fungi, and improvements in bioreactors and bioprocesses involved the fungal treatment of dye wastewater. It also discusses alternatives and perspectives for the innovation of mycoremediation to treat dye wastewaters.
Cell Cycle Arrest of Extract from Artemisia annua Linné. Via Akt-mTOR Signaling Pathway in HCT116 Colon Cancer Cells
Kim, Bo Min ; Kim, Guen Tae ; Lim, Eun Gyeong ; Kim, Eun Ji ; Kim, Sang Yong ; Ha, Sung Ho ; Kim, Young Min ;
KSBB Journal, volume 30, issue 5, 2015, Pages 223~229
DOI : 10.7841/ksbbj.2015.30.5.223
In this study, extract from Artemisia annua in L. (AAE) is known as a medicinal herb that is effective against cancer. The cell cycle is regulated by the activation of cyclin-dependent kinase (CDK)/cyclin complex. We will focus on regulation of CDK2 by cyclin E. cyclin E is associated with CDK2 to regulate progression from G1 into S phase. Akt is known to play an important role in cell proliferation and cell survival. Activation of Akt increases mTOR activity that promotes cell proliferation and cancer growth. In this study, we investigated that AAE-induced cell cycle arrest at G1/S phase in HCT116 colon cancer. Treatment of AAE shows that reduced activation of Akt decreases mTOR/Mdm2 activity and then leads to increase the activation of p53. The active p53 promotes activation of p21. p21 induces inactivation of CDK2/cyclin E complex and occurs cell cycle arrest at G1/S phase. We treated LY294002 (Akt inhibitor) and Rapamycin (mTOR inhibitor) to know the relationship between the signal transduction of proteins associated with cell cycle arrest. These results suggest that AAE induces cell cycle arrest at G1/S phase by Akt/mTOR pathway in HCT116 colon cancer cell.
Recent Advances in MALDI-MS Based Quantitative Targeted Glycan Analysis
Kim, Kyoung-Jin ; Kim, Yoon-Woo ; Hwang, Cheol-Hwan ; Park, Han-Kyu ; Jeong, Jae Hyun ; Kim, Yun-Gon ;
KSBB Journal, volume 30, issue 5, 2015, Pages 230~238
DOI : 10.7841/ksbbj.2015.30.5.230
Abnormal glycosylation can significantly affect the intrinsic functions (i.e., stability and solubility) of proteins and the extrinsic protein interactions with other biomolecules. For example, recombinant glycoprotein therapeutics needs proper glycosylation for optimal drug efficacy. Therefore, there has been a strong demand for rapid, sensitive and high-through-put glycomics tools for real-time monitoring and fast validation of the biotherapeutics glycosylation. Although liquid chromatography tandem mass spectrometry (LC-MS/MS) is one of the most powerful tools for the characterization of glycan structures, it is generally time consuming and requires highly skilled personnel to collect the data and analyze the results. Recently, as an alternative method, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS), which is a fast, robust and easy-to-use instrumentation, has been used for quantitative glycomics with various chemical derivatization techniques. In this review, we highlight the recent advances in MALDI-MS based quantitative glycan analysis according to the chemical derivatization strategies. Moreover, we address the application of MALDI-MS for high-throughput glycan analysis in many fields of clinical and biochemical engineering.
Characterization and Antimicrobial Activity against Gardnerella vaginalis of Vaginal Lactobacillus spp. Isolated from Korean Women
Kim, YongGyeong ; Kang, Chang-Ho ; Shin, YuJin ; Paek, Nam-Soo ; So, Jae-Seong ;
KSBB Journal, volume 30, issue 5, 2015, Pages 239~244
DOI : 10.7841/ksbbj.2015.30.5.239
Bacterial vaginosis (BV) is caused by microbial imbalance of the vaginal ecosystem and overgrowth of anaerobic bacteria. The antibiotic treatment often results in very high recurrence of BV because it disturbs the vaginal ecosystem. The high recurrence rates suggest a need for alternative therapeutic methods and probiotics are being recognized as alternative or additional treatment method for BV. The purpose of this study was to investigate how human vaginal isolates of Lactobacillus spp. inhibit the BV-associated pathogen Gardnerella vaginalis. Results show that selected strains significantly reduced the viability of G. vaginalis. Among these selected strains KLB410 and KLB416 were further selected based on acid/bile tolerance and identified through 16S rRNA gene sequencing being Lactobacillus plantarum. Further studies are underway to demonstrate that the selected strain can be applied as potential probiotics for recovering vaginal ecosystem.
Studies on the Enhanced Physiological Activities of Mixed Lactic Acid Bacteria Isolated from Fermented Watery Kimchi, Dongchimi
Choi, Moon-Seop ; Kim, Dong-Min ; Oh, Kye-Heon ;
KSBB Journal, volume 30, issue 5, 2015, Pages 245~252
DOI : 10.7841/ksbbj.2015.30.5.245
The aim of this study was to investigate the efficacy of enhanced physiological activities in cultures isolated from Korean fermented watery Kimchi, Dongchimi, of single lactic acid bacteria (LAB), and when these three are mixed LAB as probiotics. Using the BIOLOG system and 16S rRNA sequencing, the isolates were characterized, and identified and assigned to Leuconostoc mesenteroides DK-3, Leuconostoc dextranicum DK-6, and Lactobacillus curvatus DK-13, respectively. Growth rate and pH changes, production of organic acids as metabolites, and physiological activities of the single and mixed LAB cultures, were monitored and compared. In mixed LAB cultures after 72 h of incubation, the maximum concentrations of lactic acid and acetic acid were approximately 340.5 mM and 191.9 mM, respectively, and pH changed from 7.00 to 3.62. Mixed LAB cultures were able to eliminate 96.3% of nitrite. Activities of antioxidant and
-galactosidase were 60.3% and 16.8 units/mg, respectively. Significant antibacterial activity of the concentrated supernatants was demonstrated against several food-poisoning bacteria. Physiological activities obtained from the mixed LAB cultures have been shown to be considerably higher than those of single LAB cultures. In conclusion, these studies demonstrate that compared to the single cultures, all physiological activities in mixed LAB cultures are significantly enhanced.
Determination of Antibacterial Activity from Tricholoma matsutake Extract and Its Application to Low Salted Jeot-gal
Kim, Jin-Seong ; Park, Jae-Bum ; Jang, Seung-Won ; Kwon, Deok-Ho ; Jang, Mi-Hee ; Lee, Mi-Ok ; Ha, Suk-Jin ;
KSBB Journal, volume 30, issue 5, 2015, Pages 253~256
DOI : 10.7841/ksbbj.2015.30.5.253
The antibacterial activity from Tricholoma matsutake extracts were confirmed by minimum inhibitory concentration (MIC) test against various bacteria. Tricholoma matsutake extracts was applied for manufacturing low salted Jeot-gal with sodium DL-malate and sodium nitrite as food preservatives. Due to antibacterial activity from Tricholoma matsutake extracts, MIC of sodium DL-malate and sodium nitrite were significantly reduced to 0.025 g/L and 0.25 g/L, respectively. As a result of this study, a premium low salted Jeot-gal can be developed with low concentration of food preservatives by adding Tricholoma matsutake extracts.