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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 30, Issue 6 - Dec 2015
Volume 30, Issue 5 - Oct 2015
Volume 30, Issue 4 - Aug 2015
Volume 30, Issue 3 - Jun 2015
Volume 30, Issue 2 - Apr 2015
Volume 30, Issue 1 - Feb 2015
Selecting the target year
Present and Perspective on Insect Biotechnology
Choi, Hwan-Suk ; Kim, Sun-Am ; Shin, Hyun-Jae ;
KSBB Journal, volume 30, issue 6, 2015, Pages 257~267
DOI : 10.7841/ksbbj.2015.30.6.257
Insects are the most successful organisms on earth in terms of their diversity and adaptability. Insect biotechnology using this insect resource is an emerging area for future biotechnology with various applications. Insect resources have long been used to make food and/or functional food, feed, cosmetics as well as medicine and industrial ingredients. Recently, one of the most well-known industrial material from insect is spider silk that could be commercialize in near future. The insect cell lines have been used to express recombinant proteins that were difficult to be functional expression. For public purpose, while, the insect could be good amenity source and plant farming, so leisure resource. Only the interdisciplinary research will guarantee the successful story for insect biotechnology. And biochemical engineers should used insect as a bioresource for new products with applications in medicine, agriculture, and industrial biotechnology in near future. This review will cover state-of-the art of this field and the research and application areas of insect biotechnology and the possible role of biochemical engineer for the development of the future biotechnology using this bioresource.
Status and Prospect of 3D Bio-Printing Technology
Kim, Sung Ho ; Yeo, Ki Baek ; Park, Min Kyu ; Park, Joung Soon ; Ki, Mi Ran ; Pack, Seung Pil ;
KSBB Journal, volume 30, issue 6, 2015, Pages 268~274
DOI : 10.7841/ksbbj.2015.30.6.268
3D printing technology has been used in various fields such as materials science, manufacturing, education, and medical field. A number of research are underway to improve the 3D printing technology. Recently, the use of 3D printing technology for fabricating an artificial tissue, organ and bone through the laminating of cell and biocompatible material has been introduced and this could make the conformity with the desired shape or pattern for producing human entire organs for transplantation. This special printing technique is known as "3D Bio-Printing", which has potential in biomedical application including patient-customized organ out-put. In this paper, we describe the current 3D bio-printing technology, and bio-materials used in it and present it`s practical applications.
Antioxidant Activity of the Halophyte Ligustrum japonicum
Baek, Seung Oh ; Kim, Hojun ; Jeong, Heejeong ; Ju, Eunsin ; Kong, Chang-Suk ; Seo, Youngwan ;
KSBB Journal, volume 30, issue 6, 2015, Pages 275~282
DOI : 10.7841/ksbbj.2015.30.6.275
Dried samples of Ligustrum japonicum were extracted twice: with methylene chloride and with methanol (MeOH), respectively. The combined crude extracts were successively fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions by liquid-liquid partition. Antioxidant activities of crude extract and its solvent fractions were evaluated by measuring authentic
generated from 3-morpholinsydnonimine (SIN-1) as well as degree of occurrence of intracellular ROS in HT 1080 cells, and genomic DNA oxidation. The 85% aq.MeOH and n-BuOH fractions exhibited the good antioxidant activity. Further purification of the 85% aq.MeOH fracition led to the isolation of Oleanolic acid (1), Maslinic acid (2), and Ursolic acid (3). All compounds showed the significant antioxidant effects in all assay systems.
Optimization of Cellulase Production from Paenibacillus jamilae BRC 15-1
Cha, Young-Lok ; Yoon, Young-Mi ; Yoon, Ha-Yan ; Kim, Jung Kon ; Yang, Ji-Young ; Na, Han-Beur ; Ahn, Jong-Woong ; Moon, Youn-Ho ; Choi, In-Hu ; Yu, Gyeong-Dan ; Lee, Ji-Eun ; An, Gi Hong ; Lee, Kyeong-Bo ;
KSBB Journal, volume 30, issue 6, 2015, Pages 283~290
DOI : 10.7841/ksbbj.2015.30.6.283
In this study was selected the cellulolytic microorganism and investigated optimum condition of cellulase production for the cellulosic bioethanol production. A bacterial strain Paenibacillus jamilae BRC15-1, was isolated from soil of domestic reclaimed land. For optimizing cellulase production from the selected strain, various culture parameters were investigated such as culture medium, pH (pH 4~10), temperature (
) and culture time (2~72 h). As a result, P. jamilae BRC15-1 efficiently produced cellulase from cellulosic biomass under following conditions: 24 h of culture time (pH 7,
) in manufactured media of CMC (carboxymethyl cellulose) with peptone. Optimum saccharifying condition of crude enzyme produced from P. jamilae BRC15-1 was identified on pH 6 and
of reaction temperature, respectively. This crude enzyme from P. jamilae BRC15-1 was used for saccharification of pretreated sweet sorghum (Sorghum bicolor var. dulciusculum Ohwi) bagasse under the optimal condition. Finally, pretreated sweet sorghum bagasse including 0.1 g of glucan was saccharified by crude enzyme of P. jamilae BRC15-1 into 2.75 mg glucose, 0.79 mg xylose and 1.12 mg arabinose.
Simple Purification of BA-RGD Protein Based on CaCl
/EDTA Treatment and Inclusion Body Washing
Song, Wooho ; Byun, Chang Woo ; Yoon, Minho ; Eom, Ji Hoon ; Choi, Yoo Seong ;
KSBB Journal, volume 30, issue 6, 2015, Pages 291~295
DOI : 10.7841/ksbbj.2015.30.6.291
The limited productivity of natural shell matrix proteins has hampered the investigation of their biochemical properties and practical applications, although biominerals in nature obtained by organic-inorganic assemblies have attractive mechanical and biological properties. Here, we prepared a vector for the expression of a fusion protein of a shell matrix protein from Pinctada fucata (named as GRP_BA) with the GRGDSP residue. The fusion protein of BA-RGD was simply produced in E. coli and purified through sequential steps including the treatment with
and EDTA solution for cell membrane washing, mechanical cell disruption and the application of non-ionic surfactant of Triton X-100 for BA-RGD inclusion body washing. The production yield was approximately 60 mg/L, any other protein band was not observed in SDS-PAGE and it was estimated that above 97% endotoxin was removed compared to the endotoxin level of whole cell. This study showed this simple and easy purification approach could be applied to the purification of BA-RGD fusion protein. It is expected that the protein could be utilized for the preparation of biominerals in practical aspects.
Production of Recombinant GG1234-DsRed Fusion Protein and Its Effect on in vitro CaCO
Son, Chaeyeon ; Kim, Jin Ho ; Kim, Ji Ha ; Choi, Yoo Seong ;
KSBB Journal, volume 30, issue 6, 2015, Pages 296~301
DOI : 10.7841/ksbbj.2015.30.6.296
Eggshell-based biocomposites have become attractive due to their exquisite nanostructure and biological properties, which are mainly composed of highly organized calcium carbonate crystals controlled by organic macromolecules such as proteins and polysaccharides. Here, we designed the recombinant fusion protein of a putative eggshell matrix protein named as GG1234 and a fluorescent reporter protein of DsRed. The protein was successfully over-expressed in E. coli and purified by Ni-NTA affinity chromatography. In vitro calcium carbonate crystallization was conducted in the presence of the fusion protein, and morphological change was investigated. The protein inhibited the calcite growth in vitro, and spherical calcium carbonate micro-particles with the diameter of about
were obtained. We expect that this study would be helpful for better understanding of eggshell-based biomineralization.
Study on Antioxidant Activity of Smallanthus sonchifolius, Agrimonia pilosa, and Lithospermum erythrorhizon Extract Fractions
Kim, A-Ram ; Jeong, Gwi-Taek ;
KSBB Journal, volume 30, issue 6, 2015, Pages 302~306
DOI : 10.7841/ksbbj.2015.30.6.302
In this work, the antioxidant activity and total phenolic compound content of 6 fractions of Smallanthus sonchifolius, Agrimonia pilosa, and Lithospermum erythrorhizon extract were investigated. The highest total phenolic compound contents of each plant extracts were obtained from n-butanol (
) and methylene chloride (
) fractions (S. sonchifolius), ethyl acetate (
) and water (
) fractions (A. pilosa), and n-butanol (
) and ethyl acetate (
) fractions (L. erythrorhizon), respectively. As a result of DPPH radical scavenging activity in 10 mg/mL condition, the highest activity were obtained from n-butanol fraction of S. sonchifolius (81.06%), ethyl acetate fraction of A. pilosa (86.32%), and n-butanol fraction of L. erythrorhizon (82.6%), respectively. Also, the highest reducing power was obtained same fractions as well as DPPH adical scavenging activity. Overall, antioxidant activity has relatively closely connected with contents of total phenolic compounds in S. sonchifolius and L. erythrorhizon extracts.
Effects of Mixing Performance and Conditioned Medium on hCTLA4Ig Production in Transgenic Rice Cell Suspension Cultures
Choi, Hong-Yeol ; Park, Jun-Yong ; Nam, Hyung-Jin ; Gong, Mi-Kyung ; Yoo, Ye-Ri ; Kim, Dong-Il ;
KSBB Journal, volume 30, issue 6, 2015, Pages 307~312
DOI : 10.7841/ksbbj.2015.30.6.307
Transgenic rice cells using RAmy3D promoter can provide high productivity, and the production of recombinant protein is induced by sugar starvation. In this system, productivity was reduced during the scale-up processes. To ensure the influences of shear stress and oxygen transfer rate, working volume and mixing performances were investigated under various agitation speeds and working volumes. In addition, inoculation methods including suspended cells and filtered cells were compared. Working volumes and shaking speeds were 300, 450 mL and 80, 120 rpm, respectively. Hydrodynamic environment of each condition was measured numerically like mixing time and
. Good mixing performance and high shear stress were measured at high agitation speed and low volume. The highest level of hCTLA4Ig was 30.7 mg/L at 120 rpm, 300 mL. When conditioned medium was used for inoculation, increased cell growth was noticed during the day 0~4 and decreased slower than filtered cells. Compared with filtered cells, the maximum hCTLA4Ig level reached 37.8 mg/L at 120 rpm, 300 mL and lower protease activity level was observed. In conclusion mixing performance is critical factor for productivity and conditioned medium can have a positive effect on damaged cells caused by hydrodynamic shear stress.
Inhibition of Proliferation of Human Fibroblast by δ-Aminolevulinic Acid (ALA) Derivatives through the Induction of Mitochondria Membrane Depolarization
Jun, Yong-woo ; Han, Du-Gyeong ; Lee, Jin-A ; Jo, Su-Yeon ; Jang, Deok-Jin ;
KSBB Journal, volume 30, issue 6, 2015, Pages 313~318
DOI : 10.7841/ksbbj.2015.30.6.313
-Aminolevulinic acid (ALA) is an endogenous metabolite formed in the mitochondria from succinyl-CoA and glycine, and plays a key role in the living body as an intermediate of the compound in the porphyrin biosynthesis pathway. ALA has been commonly used in photodynamic therapy for several years, because ALA is of interest as a biodegradable mediator, a growth regulator, and an effective agent used in dermatology. Here, we determined which ALA derivatives were the most effective for the inhibition of the cell proliferation and growth of human fibroblast. As a result, we found that the treatment of ALA derivatives including ALA, ALAP (ALA phosphate salt), MAL (Methyl 5-aminolevulinate hydrochloride salt), PBGL (phophobilinogen lactam) and PBGH (phophobilinogen-HCl) could attenuate cell proliferation of human fibroblast cells. Among them, PBGH was the most effective derivative. In addition, PBGH treatment could induce mitochondrial membrane depolarization, leading to cell death of human fibroblast. These results suggest that mitochondrial membrane depolarization induced by ALA and PBGH treatment might be responsible for inhibition of cell proliferation and death. Taken together, our results propose the possibility that PBGH can be used as one of the effective drugs in human skin disease, psoriasis.
Effect of NaNO
Concentration on Microglae Arthrospira platensis Growth
Choi, Soo-Jeong ; Ha, Jong-Myung ; Lee, Jae-Hwa ;
KSBB Journal, volume 30, issue 6, 2015, Pages 319~325
DOI : 10.7841/ksbbj.2015.30.6.319
Arthrospira platensis (A. platensis) is one of the most explored cyanobacteria and has been studied for proteins, vitamins, pigment (chlorophyll and carotenoids) and fatty acid. In this study, we tested the effect of
on the microalgae growth under photoautothrophic culture in A. platensis. As a result, cell growth and dry cell weight were increased in proportion to the
concentration. Pigment (chlorophyll and carotenoids) contents of A. platensis were increased with proportion to
concentration. But, the content of pigment (chlorophyll and carotenoids) in 100%
medium of A. platensis was the highest, 40%, 140% and 200%
medium with pigment content of A. platensis was reduced. In conditions of
(40%) limitation, A. platensis could accumulate lipids to high as 1.7-fold and 1.3-fold.
Anti-inflammatory Effect of Water Extract from Tuna Heart on Lipopolysaccharide-induced Inflammatory Responses in RAW 264.7 Cells
Kim, Min-Ji ; Bae, Nan-Young ; Kim, Koth-Bong-Woo-Ri ; Park, Ji-Hye ; Park, Sun-Hee ; Cho, Young-Je ; Ahn, Dong-Hyun ;
KSBB Journal, volume 30, issue 6, 2015, Pages 326~331
DOI : 10.7841/ksbbj.2015.30.6.326
The anti-inflammatory effect of tuna heart water extract (THWE) was investigated using lipopolysaccharide-induced inflammatory response in this study. Anti-inflammatory effect was detected by the cell proliferation and the production levels of nitric oxide, pro-inflammatory cytokines such as interleukin-6 (IL-6), IL-
, and tumor necrosis factor-alpha. As a result, there were no cytotoxic effects on proliferation of macrophages treated with THWE compared to the control. The production of pro-inflammatory cytokines was remarkably suppressed compared with that of the LPS only group. These results suggest that THWE exerts the anti-inflammatory property by inhibiting production of inflammatory factors and may be a potential material for anti-inflammatory therapy.
Pretreatment and Enzymatic Saccharification of Wasted MDF for Bioethanol Production
Kang, Yang-Rae ; Hwang, Jin-Sik ; Bae, Ki-Han ; Cho, Hoon-Ho ; Lee, Eun-Jeong ; Cho, Young-Son ; Nam, Ki-Du ;
KSBB Journal, volume 30, issue 6, 2015, Pages 332~338
DOI : 10.7841/ksbbj.2015.30.6.332
The objective of this study was designed to determine the possibility of bioethanol production from wasted medium density fiberboard (wMDF). We were investigated the enzymatic saccharification characteristics using the enzyme (Cellic CTec3) after pretreatment with sodium chlorite. According to the component analysis results, the lignin contents before and after the pretreatment of wMDF (milling using sieve size of
) was significantly reduced from 31.13% to 4.11%. Therefore, delignification ratio of pretreated wMDF was found to be up to about 87-89% depending on the sieve size. And we were tested to compare the saccharification ratio according to the sieve size of wMDF (
), but it was no significance depending on the sieve size. When enzyme dosage was 5% based on the substrate concentration, enzymatic saccharification ratio was obtained up to 70% by maintaining at
for 72 hours. We could made the substrate concentration of pretreated wMDF (
) up to 12% and then enzymatic saccharification ratio was 76.8%, also contents of glucose and xylose were analyzed to 77,750 and 14,637 mg/L, respectively.
Bioactivities of Fermented Rhynchosia nulubilis in Dietary Obese Rats
Bae, Gui-jeong ; Ha, Bae-jin ;
KSBB Journal, volume 30, issue 6, 2015, Pages 339~344
DOI : 10.7841/ksbbj.2015.30.6.339
This study was to examine the lipid metabolism of fermented Rhynchosia nulubilis (FRN) in obese rats. The abnormal content of blood lipids often results in metabolic diseases, such as obesity and hyperlipidemia. Seven weeks female Sprague-Dawley rats were divided into four groups and fed high fat diets for 44 days. Also FRN was administered orally for 44 days at 7.5 ml/kg of body weight of rats. The effects of the lipid metabolism were evaluated by total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), glutamic pyruvate transaminase (GPT) and glutamic oxalacetic transaminase (GOT) levels in sera. The levels of TC, TG, LDL and GPT in FRN-treated groups were lower than those in obese groups. While HDL levels were significantly increased. These results demonstrated that FRN had improving effects of lipid metabolism in the obese rats, suggesting that FRN would be used as an ingredient of the useful functional products.
Pilot-scale Production of the Antifreeze Protein from Antarctic Bacterium Flavobacterium frigoris PS1 by Recombinant Escherichia coli with a Cold Shock Induction System
Kim, Eun Jae ; Lee, Jun Hyuck ; Lee, Sung Gu ; Han, Se Jong ;
KSBB Journal, volume 30, issue 6, 2015, Pages 345~349
DOI : 10.7841/ksbbj.2015.30.6.345
Antifreeze proteins (AFP) inhibit growth and recrystallization of ice, and permit organisms to survive in cold environments. The AFP from an Antarctic bacterium, Flavobacterium frigoris PS1, FfIBP (Flavobacterium frigoris icebinding protein), was produced in E. coli using a cold shock induction system. The culture temperature was shifted from
and a 20 L culture scale was used. The final weights of dried cell and FfIBP were estimated to be 126 g and 8.4 g, respectively. The thermal hysteresis (TH) activity (
) of the produced FfIBP was 3.6-fold higher than that of the LeIBP (Leucosporidium ice-binding protein) produced in Picha. The current study demonstrates that large-scale production of FfIBP was successful and the result could be extended to further application studies using recombinant AFPs.