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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal Basic Information
Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 4, Issue 3 - Dec 1989
Volume 4, Issue 2 - Jul 1989
Volume 4, Issue 1 - Mar 1989
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Enhancement of Oxygen Transfer in Animal Cell Culture by Using a Perfluorocarbon as an Oxygen Carrier
KSBB Journal, volume 4, issue 1, 1989, Pages 1~7
In order to increase the oxygen transfer in a bioreactor for animal cell culture, a penluorocarbon,
ppll was used in a modified Celligen
. Also, the effects of ppll on the hybridoma cell growth and on the production of monoclonal antibody were investigated. There was no harmful effect of ppl1 on the cell growth and on the production of monoclonal antibody.
Mutagenic Deactivation of 7, 12-Dimethylbenz(a)anthtacene in Nonacclimated Soil
KSBB Journal, volume 4, issue 1, 1989, Pages 8~10
Mutagenic characteristics deactivation of 7, 12-dimethylbenz(a)anthraracene was studied in a nonacclimated sandy loam soil at low and neutral pH soil conditions. Soil extracts containing transformation products were separated into three fractions based on HPLC retention time(polarity). Highly polar transformation products of 7, 12-dimethylbenz(a)anthracene demonstrated a negative mutagenic response with the Ames mutagenicity assay, strain TA-100, for both low and neutral pH soils. Moderate and low polar fractions, however, induced mutagenicity for both soil samples with mutagenic ratios similar to those of the parent compound.
Continuous Alcohol Fermentation by a Tower Fermentor with Cell Recycle Using Flocculating Yeast Strain
KSBB Journal, volume 4, issue 1, 1989, Pages 11~14
A study on the continuous fermentation with cell recycle by a tower fermentor to produce ethanol has been carried out. ethanol fermentation was conducted with flocculating yeast strain, Saccharomyces cerevisiae TS4, to compare the ethanol productivity with conventional continuous process. Employing a 15% glucose feed, a cell density of 50 g/l was obtaind. The ethanol productivity of the cell recycle system was found to be 26.5g EtOH/1-hr, which was nearly 7.5 times higher than the conventional continuous process without cell recycle. A cell recycle ratio of 7 to 8 resulted in the highest ethanol productivity and cell concentration. Thus the cell recycle ratio was found to be a key factor in controlling the production of clarified overflow liquid. An aeration rate above 3.8
10-3 VVM seemed to decrease the ethanol productivity. The continuous fermentation with cell recycle was successfully used in the separation of cells from fermentation broth with enhancement of mixing in the tower fermentor.
Radius and Length of Sephadex G Gel Fibers
KSBB Journal, volume 4, issue 1, 1989, Pages 15~16
The dimensions of dextran gel fiber described by the Ogston-Laurent gel model were estimated for Sephadex G gels. The length of fibers are ranging from
cm/ml. The radius of gel fiber and the partial specific volume of dextran gel are
cm and 0.586 ml/g, respectively.
Development of Solid-Gelatin Microcarrier for Large Scale Production of Anchorage-Dependent Animal Gell Lines
KSBB Journal, volume 4, issue 1, 1989, Pages 18~20
Solid gelatin microcarrier with the size distribution between
was prepared for the attachment and growth experiment for anchorage-dependent animal cell lines, i.e., L 929 and BHK 21. The growth and the maximum cell densities on this gelatin based and polyacrylamide (PAA) microcarriers were compared with those on the commercial dextran based Cytodex 3 microcarrier. Both cell lines showed good comparable attachment and growth patterns on the above three microcarriers. The mouse fibroblast, L 929 showed about the same maximum cell density on PAA, gelatin and Cytodex 3 MC'S BUT BHK 21, the baby hamster kidney cell line, showed the best result on Cytodex 3, which was about
cells/ml with the microcarrier concentration of 10 g/1.
Alcohol Production by Extractive Fermentation in a Continuous Bioreactor
KSBB Journal, volume 4, issue 1, 1989, Pages 21~30
Lauryl alcohol was used as extracting solvent of ethanol, and its toxicity on the free cells or immobilized cells was tested. To increase ethanol productivity, extractive fermentation method combined with ethanol fermentation and ethanol recovery was applied to the immobilized batch and continuous fermenter. As the concentration of LaOH was increased, the lag phase became longer, but specific growth rate did not change greatly. And a cell entrapment technique could protect the yeast cells against both substrate inhibition and solvent toxicity. When the glucose concentration was 400 g/l and the LaOH/fermentation medium ratio was 4, total ethanol productivity increased with the enhancement of LaOH volume, and maximum productivity was 2.75 g/l.hr in the immobilized batch fermentation.
The Effect of Hybridoma Growth Rate on the Production of Monoclonal Antibodies
KSBB Journal, volume 4, issue 1, 1989, Pages 31~33
The effect of growth rate change on glucose consumption and Ammonia production rate in batch culture of hybridoma was studied. The methods regulating growth rate of hybridoma were 1) decrease of serum concentration, 2) decrease of culture temperature and 3) addition of growth inhibitor (thymidine). The experimental results showed that hybridoma growth rate was dropped by 20~50%, while glucose consumption and ammonia production rate was decreased up to 40% On the other hand, the final concentration of monoclonal antibody was shown to be increased as high as 100% when the concentration of serum was decreased from 2% to 0.2%.
System Analysis for Mass Cultivation of Mammalian Cells to Produce Erythropoeitin(EPO)
KSBB Journal, volume 4, issue 1, 1989, Pages 34~39
Growth kinetics of mammalian cell, Chinese Hamster Ovalry(CHO) was investigated to effectively produce pharmaceutically important Erythropoeitin under perfusion chemostat conditions. Perfusion rate, D is correlated with total viable is to be an essential factor in controlling growth kinetic parameters under this kind of operations. It is also found that the measurement of oxygen uptake rates is a relatively accurate method to understand cell growth, in case that the traditional cell count method is no longer useful due to heavy cell clumpings. True growth yield, Ymax and maintenance coefficient, me associated with mammalian cell growth were estimated as
cells/ g of glucose and 0.0063 g of glucose/ cells/ hr, respectively.
Changes in some Compounds by Heat Treatment of Green Tea
KSBB Journal, volume 4, issue 1, 1989, Pages 40~47
Changes of principal components of crude green tea were determined after 30 min. of heat treatment at 10
. Four kinds of free sugars(sucrose, fructose, glucose, raffinose) and an unidentified sugar compound were separated in green tea by using High Performance LiQuid Chromatography (H.P.L.C.). 26-34 peaks were isolated as aroma compounds of green tea by means of Gas Liquid Chromatography(G.L.C). The typical aroma component of green tea such as linalool, furfural, benzyl alcohol and 13 other substances were identified. Contents of most compounds were decreased by heat treatment. Especially contents of free amino acids, free sugars, vitamin C and tannins were decreased remarkably, while those of total nitrogen and soluble nitrogen were hardly changed. The effect of heat treatment on organoleptic quality of tea extracts were examined by sensory evaluation of which result indicated the most favorable tea was produced at 115
. The Percentages of loss in contents of total sugars, reducing sugars, vitamin C, free amino acids and tannins at 115
were 17%, 16%, 36%, 12% and 15% respectively, while those were 38%, 53%, 55%, 74% and 23% at 16
Ethanol Fermentation by K. fragilis from Jerusalem Artichoke
KSBB Journal, volume 4, issue 1, 1989, Pages 48~56
Fermentation characteristics of Jerusalem Artichoke of yeast K.fragilis CBS 1555 were investigated experimentally and quantitatively according to the change of initial sugar concentrations and initial PHs of fermentation broth. Initial sugar concentrations employed were 26, 45, 65, 105, 180, and 215g/1. And initial PHs of fermentation broth were 3, 5.5, 7 and 9. The maximum specific growth rate was observed as 0.4hr-1 at 65g/1 of initial sugar concentration. The maximum specific alcohol production rate was 1.68g/ghr at 105g/1 of initial sugar concentration Cell yield and ethanol yield represent the maximum values such as 0.14 and 0.49 respectively when the initial sugar concentration was 25g/1. The maximum of ethano1 fermentability, 97% was obtained at the initial concentrations, 26 and 45g/1. However, the maximum of total ethanol yield productivity was 2.78g/1hr when the initial concentration was 215g/1. And also the optimum PH was found 5.5 for both specific growth rate and specific alcohol production rate.
Cross Flow Filtration of Pichia stipitis by a Hollow Fiber Membrane
KSBB Journal, volume 4, issue 1, 1989, Pages 57~61
The feasibility of separating Pichia stipitis from a fermentation broth using a hollow fiber membrane was evaluated. The permeate flux was affected by such parameters as cell concentration, pH, content of antifoam agents, suction pressure, and recirculation rate. A minor effect of temperature on the flux loss was also observed. A microcomputer-aided backflush was proven effective in alleviating membrane fouling and allowing long term separation of P. stipitis from a fermentation broth.