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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 5, Issue 4 - Dec 1990
Volume 5, Issue 3 - Nov 1990
Volume 5, Issue 2 - Aug 1990
Volume 5, Issue 1 - Mar 1990
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Studies on the Production of L-Ascorbic Acid; Examination of the Metabolites Produced by Gluconobacter spp. from L-Sorbitol, L-Sorbose or D-Glucose
KSBB Journal, volume 5, issue 1, 1990, Pages 1~8
G. Melanogenus metabolized D-sorbitol to L-sorbose, and to 2-keto-L-gulonic acid. G. cerinus oxidized D-glucose to accumulate 2-keto-D-gluconic acid, 5-keto-D-gluconic acid and 2,5-diketo-D-gluconic acid. 2,5-Diketo-D-gluconc acid was confirmed to be the further oxidized product of 2-keto-D-gluconic acid. The amount of calcium carbonate added to the culture broth increased the relative amount of 5-keto-D-gluconate. When, instead of calcium carbonate, other bases were employed to neutralize the oxidized products, 2-keto-D-gluconate was produced only.
Effects of p variation and phosphate limitation on the continuous fermentation of Clostridium acetobutylicum
KSBB Journal, volume 5, issue 1, 1990, Pages 9~17
The growth and fermentation profiles of Clostridium acetobutylicum KCTC 1037 were examined in batch and continuous modes with pH variation and phosphate limitation. Clostridium acetobutylicum KCTC 10 37 grew better at pH 4.5 than at pH 5.5 or 6.5. Acetate and butyrate were produced at pH 5.5, whereas culture at pH 4.5 produced acetone and butanol. Solvent production was increased by the phosphate limitation in a batch culture, but in a phosphate-limited continuous culture for 400 hours steady-state solventogenesis was not observed. The induction and maintenance of solventogenesis presumably require not only acidic condition or phosphate limitation but also favourable bioenergetic condition.
Purification of Cholesterol Esterase from Aeromoans SP.
KSBB Journal, volume 5, issue 1, 1990, Pages 19~23
A cholesterol esterase-producing microorganism, strain CES 506, isolated from soil was identified as Aeromonas sp. This strain produce about 0.023 units of cholesterol esterase per ml of culture broth. The cholesterol esterase produced by this strain was purified, 370 fold to homogeneity in an overall yield of 24% from culture broth. The apparent molecular weight was 64, 000, as estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The enzyme showed a high substrate specificity for cholesterylpalmitate and the Km value for the hydrolysis of cholesterylpalmitate by this enzyme were 0.15mM.
A Study on the Production of Xanthan Gum by Xanthomonas campestris
KSBB Journal, volume 5, issue 1, 1990, Pages 25~35
In the Xanthan gum fermentation by Xanthomonas campestris there are problems of the large energy consumption by long fermentation time, the mass transfer of oxygen and nutrients by high viscous fermentation broth. In this study, the media optimization and the fed batch fermentation were carried out to decrease fermentation time and increase Xanthan gum yield. The
uptake rate (OUR) and
evolution rate(CER) which were obtained from the analysis of fermentation exit gas using a gas chromatograph were investigated. As a result, the fermentation time decreased at optimal assimilable nitrogen concentration but increased at poor or rich assimilable nitrogen concentration, the Xanthan gum biosynthesis was stimulated under the limited condition of assimilable nitrogen source and the optimum fermentation medium was obtained as follow; Glucose=30g / l, Peptone=8.0g / l,
, Sodium acetate=20g/l, Sodium pyruvate=0.5g/1. As the agitation speed and nitrogen concentration increased, the
uptake rate and
evolution rate increased. The OUR and CER were 37.3mmol
hr and 20.2 mmol
hr at peptone 11g / l and agitation speed 990RPM, respectively. In fed batch fermentation, the final concentration of Xanthan gum was enhanced up to 29g / l.
Automatic Measurement of Cell and Glucose Concentration
KSBB Journal, volume 5, issue 1, 1990, Pages 37~42
In on-line measurement, there were two difficult problems to remove the biomass in the sample and to prevent the contamination at the sampling. In this study, the problem of the contamination at the sampling was solved by using the sampling device consisting of sterilizable sampling line, peristaltic pump and three-way valve. The glucose concentration was measured by glucose calibration equation without removing the biomass. Xanthan fermentation was performed satisfactorily using this automatic analyzing device.
Studies on the Multiplication and Induction of Hybrid Plant in Cremastra appendiculata Use the the Embryo and Tissue Culture
KSBB Journal, volume 5, issue 1, 1990, Pages 43~47
It was determined optimal Culture conditions and suitable growth regulators for seed germination, growth of callus, and protoplasts derived from cultured and mesophyll cells in Gremastra appendiculata. Induction of fusion between protoplasts of cultured and mesophyll cells was examined. The best conditions of seed germination and growth of callus were achieved on Hyponex medium contained plant growth regulators(2mg/l 2, 4-D, lmg/l Kinetin). Viability and regeneration of cell wall in protoplasts was determined with fluorescence microscope. Also, fused protoplasts were achieved by using PEG solution between protoplasts of cultured and mesophyll cells.
Extracellular Production of Alpha-Interferon by Recombinant Escherichia coli : Part I. Construction of Expression Vectors
KSBB Journal, volume 5, issue 1, 1990, Pages 49~58
We constructed hybrid plasmids to allow controlled and extracellular production of human alpha-interferon in Escherichia coli. The hybrid plassmids were constructed by transferring alpha-lFN gene from plasmid Hif-2h which has the alpha-lFN gene at PstI restriction site of pBR322, to plasmids pIN -IIIB3 and pIN-IIIC3 at restriction sites between HindIII and BamHI. Plasmids pIN-IIIB3 and pIN-IIIC3 carry E. coli lipoprotein promoter, lac promoter and operator in tandem. The plasmids also have lacl genes which encode for lac repressors, which allows controlled expression of genes cloned to the plasmids by using of inducer IPTG. Lipoprotein signal sequence is located just ahead of cloning sites of the plasmids, which helps cells to excrete or secrete cloned gene products. Plasmid pUC9 was used as a intermediate vector for transferring of alpha-lFN gene from Hif-2h to pIN vectors in order to solve the problem of different restriction sites between Hif-2h and pIN vectors.
Design of Recycle Bubble Column Reactor for Continuous Enzymatic Hydrolysis of Cellulose
KSBB Journal, volume 5, issue 1, 1990, Pages 59~67
Enzymatic hydrolysis of insoluble cellulose was performed in a bubble column with tangential flow ulrafiltration membrane unit. The reactor was operated in a batch mode as well as semi-continuous and continuous with continuous removal of products through the tangential flow ultrafiltration membrane. The optimum superficial gas velocity was 1-3cm / sec so as to avoid bubble coalescence and enzyme denaturation. In continuous and selni-cotinuous process, the conversion was gradually increased but the total reduced sugar concentration was drcastically dereased with the dilution rate. It was concluded that the bubble column attaching tangential flow ultrafiltration membrane unit was effective on continuous hydrolysis of cellulose and recovery of enzyme.
Dynamic Characteristics for the Separation of Inulin Solution in Membrane Filtrsition System of Tangential Flow
KSBB Journal, volume 5, issue 1, 1990, Pages 69~74
Dynamic characteristics of separation in membrane filtration system of tangential flow was investigated to find the functional relationship among the filtrate flux, transmembrane pressure, inulin concentration and recirculation rate. In case that NMWL is 1000, tracts-membrane pressure
, inulin concentration lwt% to 5wt%, and recirculation rate 4ml / sec, mathematical model for the function among filtrate flux, transmembrane pressure, and inulin concentration was deduced and expressed as follows.Jv = (0.0022p + 0.0003) ln
The values calculated by the above equation and those measured were compared to find to be nicely in accord with each other. Especially the agreement was enhanced in the region of higher concentration of inulin.
Ethanol Fermentation Characteristics of K. marxianus on Jerusalem Artichoke tuber Extract
KSBB Journal, volume 5, issue 1, 1990, Pages 75~80
The investigations of specific growth rate, specific alcohol production rate, cell yield, alcohol yield of K. marxianus LG were performed according to the sugar concentrations, 50, 80, 110, 190, and 250g/l of extracted solution of Jerusalem Artichoke. The functipnal relationship between specific growth rate, specific alcohol production rate, and alcohol concentrations were devoted study to. In case of low concentration of alcohol, the fuctions were linear relationships. But in the region of high concentration of alcohol, they expressed the exponential relationships. The growth rate of K. marxianus was prohibited at higher than 50g/l of alcohol concentrations regardless of concentration of residual sugar. Cell and alcohol yield showed the maximum values around 25g/l of alcohol concentraton without being related to initial sugar concentrations.
The Extraction of Lignin and Production of Vanillin from Rice Straw
KSBB Journal, volume 5, issue 1, 1990, Pages 81~85
Lignin was extracted from the rice straw by using the solvent mixture of buthyl alcohol and distilled water. And the experiment of vanillin production from extracted lignin was performed with the oxidation catalysts; CuO, Cu(OH)2 and CuSO4.5H2O. The optimum conditions of lignin extraction are the reaction temperature 12
and the mixture of 250mL buthyloloohol, 250mL, distilled water and 25g rice straw in the presence of 2.5g p-toluenesulfonic acid. The yield of vanillin from extracted lignin increased linearly with the increase of reaction temperature. And it increased with the order of Cu(OH)<
Oas oxidation catalysts. The maximum yield of vanillin was 9% in the presence of 2.5%(w/v) CuSO
O under the following conditions: temperature, 18
; pressure, 13atm; pH 4.0 and reaction time, two hours.
Enhancement of Hybridoma Cell Growth and Anti-Hepatitis B Surface Antigen Monoclonal Antibody Production in Enriched Media with Low Serum
KSBB Journal, volume 5, issue 1, 1990, Pages 87~94
Enhancement of hybridoma cell growth and monoclonal antibody(MAb) production by the addition of a small amount of serum into both serum-free medium and enriched medium was studied. The enriched medium was constructed by mixing a basal serum-free medium and a nutrient-fortified RPMI 1640 medium. It was supplemented with human serum albumin, insulin, transferrin, and monoethanolamine. It was found that addition of low concentration of serum with other serum-free supplements was favorable for growth of a mouse hybridoma 2c3.1 cells. The concentration of serum was determined to 0.5%. The maximum cell concentration obtained in this enriched medium supplemented with 0.5% fetal bovine serum (FBS) was
cells/ml and the concentration of secreted anti-Hepatitis surface antigen (antiHBsAg) MAb was
in RPMI 1640 medium with 10% FBS and
in previously-developed serum-free medium. The 2c3.1 cell growth and MAb production could be enhanced considerably by using the enriched medium supplemented with 0.5% FBS and serum-free supplements instead of RPMI 1640 medium or serum-free medium. The enhancement in MAb production in the enriched medium was more noticeable.