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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 5, Issue 4 - Dec 1990
Volume 5, Issue 3 - Nov 1990
Volume 5, Issue 2 - Aug 1990
Volume 5, Issue 1 - Mar 1990
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Process Development for Alcohol Production by Extractive Fermentation( I ) - Effect of Phase Forming Polymer -
KSBB Journal, volume 5, issue 2, 1990, Pages 95~100
Ethanol fermentations of inulin by K.fraglis in aqueous two-phase system of PEG/Dextran were performed not only to investigate the characteristics of partition of ethanol, sugar, and cell in the upper phase and the bottom phase but also to compare the fermentation properties with those of single phase system. In the range of 1 to 3 wt% of Dextran, ethanol fermentability and ethanol productivity reduced according to the increase of the molecular weight of PEG. But the cell yield and the cell productivity showed the opposite trend. In the region of 6 to 10 wt% of PEG, the increase of the concentration of PEG, caused the minute decrease of ethanol productivity but the remarkable augmentation of cell productivity. According to the increase of the molecular weight of PEG, the partition coefficient of inulin slightly decreased. But with the increment of the concentration of Dextran, the partition coefficient and the partition yield of inulin in the bottom Phase represented the trends of increase.
A Study on the Synthesis of Aqueous Biopolymer
KSBB Journal, volume 5, issue 2, 1990, Pages 101~106
The aspects of pullulan production by Aureobasidium pullulans were investigated under various initial pH, carbon source and nitrogen source conditions. The resulting pullulan fermentation broths were analyzed by using GC, LC and GPC techniques. The maximum pullulan production was obtained in the culture medium containing 5% sucrose at pH 6, 28
after 7 days of cultivation. Under the pH 3, pullulan was almost not produced although the total cell mass of A. pullulans was increased, and the case on using (NH4)SO4 as a nitrogen source, which usually cause the fermentation medium under pH 3, also gave the similar phenomena. Sucrose was believed to converted to trisaccharide and glucose extracellulary and polymerization of glucose was proceeded intracellulary.
A Study on Enzyme Immobilization on Porous Silica
KSBB Journal, volume 5, issue 2, 1990, Pages 107~112
The kinetic characteristics of an invertase immobilized covalently on porous silica have been appraised for the applicability of porous silica to immobilization supports of enzymes. The invertase was covalently bound with glutaraldehyde on 3-aminopropyltriethoxy-silane-activated porous silica to give a maximum loading of 120mg invertase per 1 gram of dry silica and 26.9 to 70.2% retention of original activity. The porous structure of silica seems to be suitable for enzyme immobilization, judging from the observed results of high immobilization capacity and comparably satisfactory retention of enzyme activity.
Effect of Histamine on the production of Interleukin-1 from Macrophage-like Cell Line
KSBB Journal, volume 5, issue 2, 1990, Pages 113~118
This experiment was carried out to investigate the immuno-regulatory effects of histamine on IL-1 synthesis and Ca2+ uptake in P388Dl macrophage-like cell line. The addition of histamine (10-8-10-3 M) increased IL-1 production in P388D1, cells, in a dose dependent manner, the treatment of EGTA (10-7-10-4M) and Co2+ ion (10-5-10-4M) decreased macrophage-derived IL-1 activity, and the pretreatment of histamine at the peak of 10-4M significantly enhanced Ca2+ uptake to P388Dl Cells. These results suggested that exogenous histamine was effective on IL-1 production from macrophage and the intracellular Ca2+ uptake play a important role in histamine-stimulated IL-1 synthesis.
Characterization of Chinese Cabbage Phospholipase D by a Multistirring Batch System Bioreactor
KSBB Journal, volume 5, issue 2, 1990, Pages 119~124
Phospholipase D catalyzes the phosphatidohydrolysis and transphosphatidylation of phospholipid in the biological systems. In this study we were partially purified phospholipase D from Chinese cabbage and the characterization of the enzyme was carried out in a multistirring batch system bioreactor. The enzyme showed optimum activity at pH ,5.6, highest activity at 37
and Ca2+ is important for the enzyme activity. Optimum concentrations of Ca2+ for phosphatidohydrolysis was 20 mM and for transphosphatidylation was 40 mM, respectively. Some organic solvents such as diethylether, isopropylether and butylacetate were activated the enzyme activity. On the other hand, EDTA, Ba2+, Mn2+ and Zn2+ showed inhibitory effect on the enzyme activity. The base acceptors in transphosphatidylation by the Chinese cabbage phospholipase D were tested. Various poly-and monohydroxy alcohols were found to be active.
Kinetics of L-Phenylalanine Production by Corynebacterium glutamicum
KSBB Journal, volume 5, issue 2, 1990, Pages 125~131
Microbial production of L-phenylalanine using Corynebacterium glutamicum ATCC 21674, a tyrosine auxotroph resistant to aromatic amino acid analogues, has been studied and kinetic analysis was performed. Even though the strain was reported as a tyrosine auxotroph, it produced tyrosine and was able to grow on the minimal medium where no tyrosine was present. The average specific growth rate at the exponential growth phase was 0.087 hr-1. There was a dissociation of growth from the formation of the product. Linear correlation between biomass production and total CO2 production was obtained. The relationship between CO2 evolution rate and sugar consumption rate was also found to be linear.
Parameter Estimation in Enzymatic Reaction Model
KSBB Journal, volume 5, issue 2, 1990, Pages 133~139
A simple and convenient method was introduced to determine the kinetic parameters for various enzymatic reaction kinetics. The method based on integrated formular can be applied to the parameter estimations from a single experiment. A modified three-parameter model was applied for the parameter estimation in reversible reaction and the equilibrium substrate concentration could be also estimated. It is possible to identify the enzymatic reaction pattern by inspecting the parameter values and the square of the correlation coefficient.
-glucosidase and properties of Immobilized Enzyme
KSBB Journal, volume 5, issue 2, 1990, Pages 141~149
-glucosidase derived from Aspergillus niger was immobilized by (1) covalent linkage on chitin and chitosan with glutaraldehyde, (2) adsorption on DEAE-cellulose and Amberite IRA93 after succinylation, and (3) entrapment on alginate and polyacrylamide gels with various cross linking agents. The retention yield of
-glucosidase immobilized on chitosan was 31.5% and operational stability was 69% after continuous operation at column reactor(5
at pH 4.8) for 15 days. The retention yield and operational stability were 24.7% and 60% respectively, in adsorption on Amberite IRA 93. On the other hand, the entrapment method by alginate and polyacrylamide gel was identified to be not appropriate due to the continuous elution of inlmobilized
-glucosidase. Optimum conditions for the immobilization on chitosan were also studied with optimum pH of 4.8 and glutaraldehyde concentration of 0.4%(w/v). The properties and stability of immobilized
-glucosidase are also investigted. The conversion yield of cellobiose to glucose was also analyzed using the column type enzyme reactor to evaluate the effectiveness of immobilized enzyme.
Changes of Enzyme Activity in Nitrogen Metabolism on Induced Association of N. muscorum with Cultured Tobacco Cells
KSBB Journal, volume 5, issue 2, 1990, Pages 151~158
Investigations on the liability of nitrogen usuage by Nostoc muscorum that has nitrogen fixing ability, and cultured tobacco cells as they were associately cultured on nitrogen-free media and effects of polyamine on the associated culture condition were carried out. In addition, measurement on the activity of nitrate reductase, glutamine synthetase, glutamate dehydrogenase and glutamate synthase that take part in the metabolic pathway of nitrogen fixation product were performed. Among enzymes participating in the metabolic pathway of nitrogen fixation products, the activity of nitrogen reductase stimulated five times in associated culture, and that of glutamine synthetase of N. muscorum increased two times after heterocyst differentiated. Activity of glutamate dehydrogenase increased markedly when cultured tobacco cells were solely incubated on nitrogen-free media, but inhibited when cultured associately. And, glutamate synthase was showed the highest activity in 0.1 mM of spermine treated group.
Ethanol Fermentation by Cell Recycle Fermentor with a Fabric Filter
KSBB Journal, volume 5, issue 2, 1990, Pages 159~165
Ethanol fermentation by Scccharomyces cervisiae was carried out in the cell recycle filter system with a cheap fabric filter having a pore size of 10
m. Maximum biomass concentrations up to 85g/1 were obtained, but in practice operational concentrations were between 50 and 80g/1. Ethanol productivity was 42g/1-hr, with an ethanol concentration of 66g/1 and an ethanol yield of over 86%. Continuous operation was possible by applying periodic backflushing. The ethanol fermentation could be carried out without difficulty at a dilution rate up to 0.8h-1 In order to obtain a high cell concentration and ethanol productivity, development of filter module with the larger filtration area is required.
Vinegar Production by Acetobacter aceti Cell Immobilized in Calcium Alginate
KSBB Journal, volume 5, issue 2, 1990, Pages 167~173
This study is to investigate for obtaining the operating conditions of continuous vinegar production using fluidized bed reactor by Acetobacter aceti cell immobilized in Ca-alginate gel. The optimum conditions obtaining by batch fermentation using fluidized bed reactor were as follows; The fermentation temperature and aeration rate were 3
and 1.0VVM and the initial concentration of ethanol and acetic acid in medium were 33g/l and 27g/l respectively. The amount of bead used was 25%(w/v). The overall acetic acid productivities of batch fermentations by free cell and immobilized cell were 0.31g/l-hr and 0.48g/l-hr, respectively, at the final acetic acid concentration of 50g/l. In the continuous vinegar production using fluidized bed reactor by immobilized cell under optimum conditions, it was possible to produce 23g/l acetic acid continuously up to 90 days with maximum acetic acid productivity of 2.76g/l-hr at dilution rate 0.12hr-1.
Somatic Hybrids by Electro-Protoplast Fusion between N. tabacum and N. glutinosa
KSBB Journal, volume 5, issue 2, 1990, Pages 175~182
Protoplasts, isolated from leaf of N. tabacum NR-/SR+ and N. glottnosa were electrofused and divided with a plating efficiency of 30∼35% in AAPI 9M medium. Green callus lines were selected in protoplast-derived colonies on MSNO3 selection medium with 1.2mg/ml streptomycin sulfate on the basis of nitrate reductase proficiency and streptomycin resistance. Four putative hybrid plant lines regenerated from the green callus lines had intermediate morphology between that of parents with respect to floral shape, corolla length and ovate leaf blade. Zymograms of leaf peroxidase and esterase from these putative hybrid plant lines showed isozyme profiles derived from both parents and also, they exhibited additional and lost bands. Cytological analysis of two putative hybrid plant lines gave chromosome counts of 2n=66 in L22 and 2n=54 in L44 which were less than the expected number of N. tabacum(2n=48) and N. glutinosa(2n=24).
A Simplified Procedure for the Large-Scale Purification of Urokinase from Human Urine
KSBB Journal, volume 5, issue 2, 1990, Pages 183~189
An efficient method has been developed for the purification of urokinase from 1, 000 liter batches of human urine. The procedure involved precipitation of urokinase with 2mM zinc chloride, resuspension of the precipitate with 0.1M EDTA/0.5M Glycine solution, and CM-Toyopearl and benzamidine-Sepharose column chromatography. The purified urokinase was fully active and possessed a specific activity of 1.07
105IU/mg. The recoveries ranged from 42 to 65% in several preparations(mean value was 51%). And the urokinase purified by this process consisted of about 13% of single chain urokinase (pro-urokinase) as evaluated by SDS-polyacrylamide gel electrophoresis in reducing condition and by S-2444 amldolytic activity under plasmin treatment.
Assay Method of L-tyrosine and L-DOPA Mixture Using Spectrophotometer
KSBB Journal, volume 5, issue 2, 1990, Pages 191~194
Tyrosine is a monohydrolic aromatic amino acid and DOPA is a tyrosine derivative containing dihydroxy group. DOPA can be synthesized from tyrosine by enzymatic reaction. The separation and quantitative determination of each component are very difficult in the reaction mixture. In the present study, two wavelengths giving maximum absorbance difference of each amino acid were determined using UV/VIS spectrophotometer by wavelength scanning and simple assay method was developed for the analysis of the reaction mixture of tyrosine and DOPA by measuring absorbances of reaction mixture. This method can be simply used for the analysis of the tyrosine and DOPA mixture because it does not require and procedure for the pretreatment of the reaction mixture.