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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal Basic Information
Journal DOI :
Korean Society for Biotechnology and Bioengineering
Editor in Chief :
Volume & Issues
Volume 5, Issue 4 - Dec 1990
Volume 5, Issue 3 - Nov 1990
Volume 5, Issue 2 - Aug 1990
Volume 5, Issue 1 - Mar 1990
Selecting the target year
Production of Rabbit Anti-Bovine Heart Pyruvate Dehydrogenase Antibody
KSBB Journal, volume 5, issue 4, 1990, Pages 315~321
Rabbit anti-bovine heart PDH antiserum was raised against El(a, b) isolated from PDC, and then applied to detect Ela and Elb. Appropriate amounis of El were fractionated by SDS-PAGE and electrophoretically transferred to nitrocellulose membrane. The Ela and Elb on the membrane were incubated with anti-El antiserum and identified by GAR-HRP system. It has been found that the immunodetection sensitivity of Ela and Elb were directly proportional to the amount of antigen and transfer time. The lengthy transfer times increased the immunodetection sensitivity of Ela and Elb. The maximal detection sensitivity of Western blotting of Ela and Elb was achieved at 3.5 V/cm for 16-hour transfer under these experimental conditions.
Studies on the Induction of Transformation in Cereal Plants.II. Expression of Gene Transferred into Rice Protoplasts by Electroporation
KSBB Journal, volume 5, issue 4, 1990, Pages 323~327
Protoplasts isolated from embryogenic cell suspensions were electroporated in buffered solutions containing plasmid DNA of pBI121. Transient GUS (beta-glucuronidase) activity measurement and selection for kanamycin resistent showed that expression of foreign genes and stable loransformation were achieved. GUS transient gene expression was increased by increasing DNA concentration of pBI121 plasmid and affected by the level of the applied voltage. An optimal level of GUS activity was obtained after electroporation with a pulse of 200 voltage/1180 uF. Protoplast viability was up to the 60% at the optimal voltage. Cell colonies resistent to 200
/ml kanamycin were selected in agar medium and identified by histochemical GUS assay.
Continuous Ethanol Production from Starch by Simultaneous Saccharification and Fermentation in a Tapered Column Fermentor
KSBB Journal, volume 5, issue 4, 1990, Pages 329~334
In an attempt to develop a novel process for ethanol production from starch, a simultaneous saccharification and fermentation (SSF) process using Zymomonas mobilis and amyloglucosidase (AMG) was studied in continuous modes. Compared with a conventional cylindrical column type of fermentor, the tapered column type of fermentor was found to be superior in terms of reactor performance for ethanol fermentation. The tapered columm fermentor packed with coimmobilized Z. mobilis and AMG alleviated the problems which were associated with CO2 evolution and provided a significantly better flow pattern for both liquid and gas phases in the fermentor without channelling. However, the fluidized bed type of tapered column fermentor using flocculent strain of Z. mobiles and immobilized AMG showed lower productivity (5.2g/1/h) than that of packed bed type of tapered column fermentor(9.2g/l/h).
Semibatch Ethanol Production from Starch by Simultaneous Saccharification and Fermentation Using Cell Recycle
KSBB Journal, volume 5, issue 4, 1990, Pages 335~339
In order to develop economic processes for ethanol production from starch, a simultaneous saccharification and fermentation(SSF) process using Zymomonas mobilis and amyloglucosidase (AMG) was studied in semibatch modes using cell recycle. The cell recycle was carried out by adopting two different methods; microfiltration and settling. The cell recycle using microfiltration revealed higher productivity(5.4 g/l/h) than that using a settler(4.3 g/l/h). Taking the large-scale ethanol fermentation into account, the semibatch process using microfiltration system appeared most promising among others with respect to ethanol productivity, feasibility of scale-up and simplification of operation.
On the Compsition of Triglyceride in the Oil of Walnut and Pine-nuts
KSBB Journal, volume 5, issue 4, 1990, Pages 341~345
The composition of triglyceride in the oil of walnut and pine-nut were analysed quantitatively by Tri-analysis column of HPLC. The results were as follows: 1. The compositions of triglyceride of pine-nut oil were
in order, respectively. 2. The compositions of triglyceride of walnut oil were
in order, respectively.
Effects of Nutrients and Cell Aggregate Size on the Biosynthesis of Carotenoid in Daucus carota Suspension Culture
KSBB Journal, volume 5, issue 4, 1990, Pages 347~353
The effects of nutrients and cell aggregate size on the cell growth of D. carota and the biosynthesi of carotenoid were investigated. Highest carotenoid content was obtained with sucrose as a carbon source and the equal ratio of ammonium to nitrate. High phosphate concentration stimulated the carotenoid biosynthesis in D. corota. 2,4-D inhibited the cell growth but stimulated the specific carotenoid content at high concentration. By modifying the medium composition based on these findings, three times higher specific carotenoid content and 2.5 times higher total carotenoid content were obtained as compared with the results obtained with basic MS media. Biosynthesis of carotenoid was found to be affected by cell aggregate size; high carotenoid production was obtained from the large aggregated cells resulted from high sucrose concentration.
The Medium Optimization through Continuous Culture of an Methanol Utilizing Bacterium for SCP Production
KSBB Journal, volume 5, issue 4, 1990, Pages 355~363
Methanol-utilizing bacterium isolated from sewage samples in Seoul showed optimal temperature and pH of
and 7.1 for growth, respectively. The maximum specific growth rate was
. The minimum medium composition was reconstituted depending on the surplus and the deficit of each component in the basal medium at steady state. The optimal composition was given as(g/l); Methanol 40,
Under the continuous culture with optimum medium the maximum cell productivity was 3.8g/1/hr at dilution rate
. Maximum cell concentration and its protein content were 19.5g/l and 70% at dilution rate of
Effects of Glucose and Glutamine Concentrations on Hybridoma Growth ann Anti-HBsAg MAb Production in Enriched Medium
KSBB Journal, volume 5, issue 4, 1990, Pages 365~371
To improve the growth of mouse hybridoma 2c3.1 secreting anti-Hepatitis B surface antigen monoclonal antibody (anti-HBsAg MAb), we had constructed an enriched medium and observed the effects of fetal bovine serum and serum-free supplements including human serum albumin, 'insulin and transferrin', and monoethanolamine. For further enhancement of growth, the concentrations of two major energy sources, glucose and glutamine, were strengthened with various ratios in the enriched medium. Maximum cell growth and monoclonal antibody production obtained in various ratios of glucose/glutamine with an inoculation concentration of 2
105 cells/ml were 0.73
106 cells/ml and 65.1-422.6
/ml, respectively. Glutamine was round to be a major energy source and a limiting nutrient in comparison to glucose for 2c3.1 cell cultivation in enriched media with low serum.
Enhancing Effect of Egg Albumin on Ethanol Production and Its Function
Kim, Heung S. ; Shin, Chul S. ; Wang, Shaw S. ;
KSBB Journal, volume 5, issue 4, 1990, Pages 373~376
In ethanol fermentations with Saccharomyces sake, phosphatidylcholine-egg albumin as a supplement in fermentation media was much more effective in enhancing ethanol production than linoleic acid-ergosterol. It came from the differences in alcohol-tolerance between egg albumin and ergosterol. The egg albumin was supposed to function as a nutrient rather than to form protective layers around the cells against ethanol.
Controlled Drug Release Using Biodegradable Polymer
KSBB Journal, volume 5, issue 4, 1990, Pages 377~382
The characteristics controlled drug release have been studied for biodegradable polymer matrix. Polymer matrix was prepared from glycerine, prednisolone and dextran. Mathematical analysis of the data showed that the release behavior actually conformed to the Higuchi's diffusion controlled model. The release time was increased as drug loading doses increased, whereas decreased as glycerine concentration increased. The release rate did not change by varying molecular weight of dextran.
Studies on Saccharification and Citric Acid Fermentation of Alcoholic Distillery Waste(I)
KSBB Journal, volume 5, issue 4, 1990, Pages 383~390
Alcoholic distillery waste was utilized as dual purposes to produce citric acid and to reduce the amount of waste to be treated. Enzyme and acid hydrolysis of this waste were studied to suggest effective way of present purpose. Enzymatic hydrolysis of this naked barley alcoholic distillery waste by
-amylase gave glucose as 8g/l concentration at
for 6 hours, which produced 1g/l citric acid and 5.33g/l mycelial. This waste material hydrolyzed with 25% HCl at
showed 21.5g/l glucose and produced 1.75g/l citric acid with 4.9g/1 mycelial. The glucose concentration was decreased to 3.44g/l by further 2nd acid hydrolysis because the monosugars were decomposed at prolonged hydrolysis conditions. The addition of 3g/l
increased the mycelial growth but reduced the amount of citric acid formed. The formation of citric acid was increased at low concentration of manganese ion.
Enhancement of scu-PA Production from Human Kidney Cells by a Novel Bioreactor
KSBB Journal, volume 5, issue 4, 1990, Pages 391~396
10-3 (IU/cells/day) of maximum specific scu-PA production, which was higher than those by a 75
T-flask and conventional perfusion systems, was maintained by tube-type bioreactor at 0.35 (1/h) of perfusion rate corresponding to 0.15 (dynes/
) of shear stress. The production of scu-PA is also increased as shear stress was slowly increased, which is similar to natural human blood circulation. The tube reactor proves that there may be no limitation of oxygen supply by showing 1.0 (1/h) of oxygen transfer coefficient at steady state and this system yields much lower shear stress of 0.3(dynes/
) than that of 5-8(dynes/
) by conventional agitation systems.
Hydrolysis of Polyfructan by Resin Catalysts
KSBB Journal, volume 5, issue 4, 1990, Pages 397~402
Hydrolysis of inulin was investigated employing various commercially available resin catalysts for the production of high grade fructose syrup. The particle size and porosity of the resin significantly affected the distribution of the products, indicating that the intraparticle diffusion of reactants controls thc selectivity as well as the reaction rate. To confirm the effect of the intraparticle diffusion, two different types of resin catalysts were prepared: the one having sulfonic acid group distributed uniformly throughout genular microparticles (A-type) and the other having sulfonic acid group located mainly at the exterior surface of genular microparticles (E-type). The results were found that the reaction rate and the selectivity of the E-type catalyst were higher than those of the A-type catalyst.
On Energy Saving and Quality Improvement of Food Process (1). Applications of Hotwire Monitoring System for Food Biotechnology
KSBB Journal, volume 5, issue 4, 1990, Pages 403~410
Application of the Rheocatch Hotwire Monitoring System for food biotechnology process was evaluated. The growth of microogranism, E coli (JM 83 and Sigma) and Corynesccfertun glutamicum, were monitored. in the fermentor. The cell growth could not be detected the temperature differences between the hotwire and samples(
T) as indicated by the monitoring system during the fermentation processes. The cell concentration of less than 2g/dl was not sufficient to generate the measurable temperature difference in the fermentor. In order to calibrate the Rheocatch Monitoring System, the temperature difference as a function of solute concentration (microbial cells, sodium cholide, sucrose and dextran) was studied. The relationship between
T and the concentration of microbial cells, sucrose and dextran can be expressed in a power series. Further studied with dextran indicated that viscosity and/or kinematic viscosity increase exponentially with an increase in
T This is regardless of the concentration and molecular weight of dextran.
T linearly increases with the logarithm of molecular weight, while the logarithm of viscosity and the logarithm of kinematic viscosity increase with the logarithm of molecular weight.
Extractive Separation of an Alkaline Protease Using Reversed Micelles
KSBB Journal, volume 5, issue 4, 1990, Pages 411~414
The extraction behavior of an alkaline protease using reversed micelles was investgated. The reversed micellar solution consisted of AOT in isooctane. It was found that distribution of arkaline protease into the organic phase increased at lower pH, lower ionic strength, and higher AOT concentration. When the real fermentation broth was extracted of alkaline protease, an activity yield of 20% and a purification factor of 2.0 were obtained.