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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 7, Issue 4 - Dec 1992
Volume 7, Issue 3 - Sep 1992
Volume 7, Issue 2 - Jul 1992
Volume 7, Issue 1 - Apr 1992
Selecting the target year
BIOLOGICAL PRETREATMENT OF HIGH ENERGY SORGHUM
KSBB Journal, volume 7, issue 1, 1992, Pages 1~7
Degradation of structural carbohydrates has been observed in samples of sweet sorghum inoculated with either Clostridium cellulolyticum or Bacteroides succinogenes. However, conditions under which these rellulolytic organisms can compete effectively with lactic acid bacteria have not yet been determined. Degradation of cellulose by B. succinogenes was found not to be inhibited by either glucose or succinate.
Performance of Cone Type Tower Fermentor for Ethanol Production
KSBB Journal, volume 7, issue 1, 1992, Pages 9~14
A cone type of tower fermentor loaded with flocculating Sacchromyces uvarum was used to study the fermentor performance. The performance of cone type fermentor was compared with those of other fermentors. The maximum yeast concentration in the cone type of tower fermentor was 35.9-43.0g/1.hr and the maximum ethanol productivity was 14.75g/1.hr at the dilution rate 0.26
. The ethanol yield was 0.446-0.472g ETOH/g Glucose. It was concluded that a cone type of tower fermentor might offer better perspectives for continous etanol fermentation.
Sorbitol production from Jerusalem artichoke by inulinase and permeabilized Zymomonas mobilis
KSBB Journal, volume 7, issue 1, 1992, Pages 15~20
The use of Jerusalem artichoke containing
-1, 2-fructose oligomer in the production of sorbitol that is used as food additives and precursor for the L-sorbose has been studied. Coimmobilization of both inulinase and oxidoreductase was considered for the simultaneous reaction for hydrolysis of inulin and conversion of glucose and fructose liberated from inulin to sorbitol. Both inulinase and oxidoreductase were immobilized in chitin(5%, w/v) and K-carrageenan(4%, w/v), The activity of oxidoreductase was specified by permeabilization of Zymomonas mobilis cell with 0.2% CTAB(Cetyltrimethylammonlumbromide). The use of inulinase for hydrolysis of inulin resulted in 36.65g/l of glucose and 85.32g/1 of fructose respectively. These are valuable substrates for sorbitol production. Using these hydrolyzates, accumulation of 35.64g/l for sorbitol occurred at
and pH6.2. When permeabilized cells and inulinase were coimmobilized, sorbitol produced at 30.15g/l although it is low compared with 35.64g/l in separated reactor system.
Investigation of Brassinosteroid Substances in Undmia pinnatifida
KSBB Journal, volume 7, issue 1, 1992, Pages 21~26
In order to examine the presence of brassinosteroid substance in sea mustard(Undaria pinnatifida), leaves of sea mustard were extracted with MeOH. The extract was purified by slovent fractionation, counter current distribution, silica gel adsorption chromatography, charcoal adsorption chromatography, Bondesil chromatography, and reverse phase HPLC, successively. The activity was monitored by the rice inclination test and its prescence could be confirmed in each purification step. Although sea mustard contained a less amount of the active substance than the vegetative tissue of higher plants, brassinosteroid was clearly present endogenously in sea mustard. We acknowledge that our work is probably the first publication reporting the presence of brassinosteroid in marine algae plants.
Ethanol Production from Xylulose by Saccharomyces cerevisiae
KSBB Journal, volume 7, issue 1, 1992, Pages 27~31
Xylose represents a major component of cellulosic materials. This paper describes patterns of ethanol fermentation by Saccharomyces cerevisiae from xylulose, which is an isomer of xylose. Special emphasis was placed on the effects of xylulose concentration and growth temperature on cell growth and ethanol yield. The maximum specific growth of
was obtained at an initial xylulose concentration of 5 g/1. The ethanol yield was propotional to initial xylulose concentrations. A xylulose concentration of 16 g/l resulted in the maximum ethanol yield of 0.49 g EtOH/g xylulose, which corresponds to 90% of a theoretical value. It is interesting to nota that xylulose metabolism was accelerated by the presence of glucose as a carbon source.
Study on the Factors Affecting the Ethanol Tolerance of Yeast Strains by fermentation Temperature
KSBB Journal, volume 7, issue 1, 1992, Pages 33~37
This study is to investigate the ethanol tolerance of yeast strains related to fatty acid composition and intracellular ethanol concentration for various fermentation temperatures. The maximum accumulation of ethanol in the cells was decreased by lowering the fermentation temperature, while unsaturated fatty acid content was increased by decreasing the fermentation temperature. Thus, we found that the increase of ethanol accumulation in cells resulted in the decrease of unsaturated fatty acid content. Therefore, it was suggested that the composition of unsaturated fatty acids in the cell membrane be strongly related to the diffusion of ethanol from cell to medium.
Immunopotentiating Effect of Polysaccharide Produced from a Mutant of Acetobacter pasteurianus (I)
KSBB Journal, volume 7, issue 1, 1992, Pages 39~44
Dose-dependent responses indicated by the increase of leukocyte, peritoneal exudate cell and weights of immunorgans revealed the improvement of immunity. In the effect of macrophage on phagocytes, there were not substantial differences in the phagocytic and corrected phagocytic index. The administered group being compared with the controlled group, there were no significant changes in SGOT, S-GPT, alaline phosphatase, total protein, albumin, globulin, cholesterol, triglyceride, blood urea nitrogen and glucose. Key words: dose-dependent responses, peritoneal exudate cell, phagocytic idex, corrected phagocytic index.
Immunopotentiating Effect of Polysaccharide Produced from a Mutant of Acetobacter pasteurianus (II)
KSBB Journal, volume 7, issue 1, 1992, Pages 45~50
Footpad swelling having the relationship with arthus-reaction of antibody-mediated hypersensitivity and delayed type hypersensitivity was recovered to the almost normal level. The nethemoglobin induced by aniline showed no significant deviation. PFC/spleen cell and PFC/106 spleen cell were increased slightly, but not in case of RFC. Hemagglutination value was increased slightly, but hemolytic value was not changed significantly. Mice in the administration of the polysaccharide does not show any significant stress factor in the cage for mice admlrustered plasma corticosterone. Key words: antibody-mediated hypersensitivity, delayed type hypersensitivity, methemoglobin, Hemagglutination value, hemolytic value, plasma corticosterone.
Biocatalysts in Reverse Micelles
KSBB Journal, volume 7, issue 1, 1992, Pages 51~58
The use of watersoluble enzymes for chemical synthesis suffers from several limitations. The solubilization of biocatalyst (Enzymes and Cells) with reverse micelles or microemulsion could be a method for bioconveision of low water soluble substrates. In this review, We will discuss the properties and the potentials of reverse micelle for catalytic bioconversion and biotechnology.
Dynamic Characteristics of External loop Air-Lift Reactor
KSBB Journal, volume 7, issue 1, 1992, Pages 59~65
Hydrodynamics and mixing characteristics such as circulation time, mixing time, circulation velocity and axial dispersion coefficient were investigated using highly viscous pseudoplastic solutions of carboxymethyl cellulose(CMC) in an external circulation loop air-lift reactor with 13
working volume. The superficial gas velocity was changed from 1.9 to 6.2cm/s and CMC concentration from 0 to 1.0wt%. The theoretical model based on the pressure balance is developed mathematically to predict liquid circulation velocity. Gas hold-up, circulation velocity and axial dispersion coefficient of liquid phase increased with increasing gas velocity and decreased slightly with increasing liquid viscosity. Mixing time and circulation time decreased with increasing gas velocity and increased with increasing liquid viscosity. Experimental data on liquid circulation velocity were in good agreement with the predicted values.
Ethanol Productivity in a Hollow Fiber Membrane Module Using High Density of Saccharomyces cerevisiae
KSBB Journal, volume 7, issue 1, 1992, Pages 67~71
We studied a continuous production of ethanol by Saccharomyces cerevisiae in a hollow fiber membrane bioreactor which consisted of 50 polypropylene fibers and 3 teflon fibers. The produced
was removed through the teflon fibers and excess biomass was removed through the shell side. We obtained the cell and ethanol concentrations of 266g/L and 205g/L based on the shell-side volume. A nitrogen deficient medium resulted in too low an ethanol productivity to be applied to a practical process.
Biosolubilization of Australian lignite: effect of pretreatment method on biosolubilization
KSBB Journal, volume 7, issue 1, 1992, Pages 73~78
The solubilizations of Australian lignite by the fungus Poria cocos, Trichodermareesei, Candide tropicalis, and niger were investigated. Three different types of chemical pretreatment methods were used for increasing biosolubility of lignite. Nitric acid and hydrogen peroxide were proven to be proper chemical pretreatment materials of Australian lignite. Poria cocos showed much better solubilization ability than other strains. Interpretation of the nature of coal solubilization by Poria cocos was based primarily on infrared, ultraviolet and nuclear magnetic resonance spectrum analyses.
Production of Palatinose by Immobilized Cells of Erwinia rhapontici
KSBB Journal, volume 7, issue 1, 1992, Pages 79~83
The characteristics of Erwinia rhapontici cells with
-glucosyltransferase activity immobilized in Ca-alginate beads and the performance of two different types of reactor-stirred tank reactor(STR) and packed bed reactor(PBR)-charged with these immobilized cells to produce palatinose from sucrose were investigated. The optimal pH(5.5-6.0) and temperature(
) showed no appreciable difference between free and immobilized cells. The apparent Km value of the immobilized cells(0.28M) was approximately two times higher than that of free cells(0.13M) at
. The half life of the immobilized cells was found to be 380 h with STR while much greater operational stability was achieved with PBR. Continuous operation of PBR at a space velocity of
for 30 days showed only 5% loss of initial activity.