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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 7, Issue 4 - Dec 1992
Volume 7, Issue 3 - Sep 1992
Volume 7, Issue 2 - Jul 1992
Volume 7, Issue 1 - Apr 1992
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The Effect of NAA and BA on Polar-Regeneration of Shoot in Hybrid Poplar
KSBB Journal, volume 7, issue 2, 1992, Pages 85~91
The role of NAA(1-Naphthaleneacetic acid) and BA(6-Benzyladenine) in the adventitious shoot regeneratlon from Populus leaf segments and changes in the pattern of RNA and protein synthesis were investigated. The adventitious shoot regeneration octured at the basal cut end of Populus leaf segments. This process was effected by many factors, including wounding culture conditions, light and plant growth regulators etc. The highest adventitious shoot regeneration frequency was obtained at
BA. In this condition adventitious shoot starved to regenerate on the 13th day of oullure. The most optimal hormone composition for RNA and protein synthesis was
BA. The content of RNA and protein was greater at the proximal part. In the course of adventitious shoot regeneration, the proteins associated with polar-regeneration appeared at the proximal part of populus leaf segment.
Development of Low-serum Medium(LSM) for Mouse-mouse Hybridoma Part I. A Study of the Role of Serum Components Using a Serum Model
KSBB Journal, volume 7, issue 2, 1992, Pages 92~95
A model for the role of serum was proposed to develop a low serum medium for the large scale cu1ture of mammalian cell. The strategy of medium development adopted in this study facilitated the understanding of the role being carried out by the serum in the culture of hybridoma KAl12 cell line. In this model, the serum components were divided into two main groups : the first group encompasses the nutrient factors that determine the maximum cell density and the second group includes the growth factors that regulate the cell growth rate, The model prediction was compared with the experimental results. The model enabled us to find out several useful aspects of medium composition for cell growth. 1) One particular component in the basal medium became limiting factor when serum concentration level was more than 7%. 2) The growth regulating factors and nutrient factors limited the cell growth at 3% and 5% serum concentration levels respectively.
Develpment of Low-serum Medium(LSM) for Mouse-mouse Hybridoma Part II. Development of Low Serum Medium by Screening for Serum Replacement
KSBB Journal, volume 7, issue 2, 1992, Pages 96~101
A low serum medium(LSM) suitable for the growth of a self-constructed hybridoma cell line, KA112, was established by selecting ingredients to replace serum. Insulin and sodium pyruvate was important for the growth of cell line KA112. Various basal media were tested and DMEM gave the most favorable result. Low serum medium (LSM) developed in this work showed cell line stability in the culture for more than 6 months and exhibited cell growth equivalent to that carried out in medium supplemented with 7% FBS. LSM was found to be applicable to the suspension culture of KA112. The reduction of serum level down to 1%(V/V) FBS in LSM resulted in a substantial saving in the cost of media preparation for large scale culture.
Effects of Culturing Parameters on the Production of Microbial Biosurfactant from Candida bombiocola
KSBB Journal, volume 7, issue 2, 1992, Pages 102~106
Effects of nitrogen souses and C/N ratio were investigated on the production of extracellular microbial surfaclant, sophorolipid, from C. bombiocola. Organic nitrogen sources, such as urea, peptone and yeast extract was found to be more effective for sophorolipid production, than inorganic nitrogen sources. Depending on the nitrogen sources, sophorolipid production pattern varied by increasing C/N ratio. Increased production of sophrolipid could be obtained up to 90g/L by feeding carbon source again 2 days after cultivation.
Application of Biosurfactant(Sophorolipid) Produced from Candida bombiocola
KSBB Journal, volume 7, issue 2, 1992, Pages 107~111
Chayacterlstics of the sophorolipid produced from Candida bombiocola were investigated as an emulsifier of oil, a detergent or as a dispersant. Improved emulsification of crude oil was observed at high temperature (
) with less than 1% concentration. Sophorolipid solution produced little foam even at reduced surface tension, however performance as a detergent of soiled cloths was poor. Dispersing and stabilizing abilities of sophorolipid solution were proved to be superior to those of chemical dispersants when examined by dispersing
or carbon black powders.
Production of Hyaluronic Acid from Streptococcus zooepidemicus
KSBB Journal, volume 7, issue 2, 1992, Pages 112~117
An optimal composition of medium for hyaluronic acid production and some characteristics of its from Streptococcus zooepidemicus were investigated. The hyaluronic acid from S. zooepidemicus was reached maximum level in the BY-medium containing 0.1% beef extract, 0.1% yeast extract, 3.0% glucose, 2.0% peptone, 0.1% NaCl and
(pH 7.5) at
for 36 hours with shaking. Addition of
to the medium was necessary to neulralize the lowered pH which was resulted from hyaluronic acid production. Molecular weights of extracelluar and cellular hyaluronic acid produced by the strain were
, respectively. The amount of extracellular hyaluronic acid was 91.9% of total hyaluronic acid produced and the vest was all intracellular.
Growth of Pseudomonas aeruginosa in Cosmetics(Emulsion-type) and the Effect of Antiseptics
KSBB Journal, volume 7, issue 2, 1992, Pages 118~125
Most of cosmetics are emulsion-type products which contain the sources of nutrition, i.e., vegetable oil, mineral oil and carbohyrate etc.. These additives are usually very susceptible to the contamination by microorganisms. The purpose of this study is to obtain the data necessary not only to prevent dermalopathia occurred by microbials but also to maintain the quality. In this experiment we observed the growth of P.aeruginosa in the cosmetics with or without antiseptics so as to prevent contamination. During the contamination period, the phase became unstable and creaming phenomina was happened together with some discoloration and bad smell. The pH of cosmetic was decreased from 7.6 to 6.0 and the concentration was increased from 1.443 to 1.453 in terms of refractive index during 40 days incubation. By adding antiseptics to the cosmetics, the number of P. aeruginosa from the challenge test method were decreased from
cell/ml. For the antibacterial effect against P. aeruginosa, p-hydroxy benzoic acid propyl ester in phosphoric acid buffer solution showed the best result.
Study on the Fermentation Conditions Influencing the Production of Vitamin
by Propionibacterium shermanii
KSBB Journal, volume 7, issue 2, 1992, Pages 126~131
The effects of fermentation conditions and medium compositions on the production of vitamin
by Propionibacterium shermanii IFO 1239 were studied. Changes from an anaerobic to aerobic condition and a complex to synthetic medium after 48hr resulted in a 100% increase in vitamin
production compared to an anaerobic culture alone. Glucose, fructose and lactose were found to be equally good as a carbon source for vitamin
production. Addition of succinate and malate to the synthetic medium with glucose as a carbon source led to an increase in vitamin
production by 33.6% and 17.2% respectively.
New Adaptation Process to Serum Free Media for the Growth of Serum-requiring Human Cell Line
KSBB Journal, volume 7, issue 2, 1992, Pages 132~138
Sprial adaptation technique of conditioned media has been applied to cultivate human cell line which can not survive in a serum free mdium without adding any growth factors in basal medium Doubling time and scu-PA production from serum free adapted cells were 5 days and 890 (IU/mL), respectively in a T-flask, whose values were not much lower than the productivity of 1100(IU/mL) from 5% serum containing medium. It was required to use conditioned media for attaching cells on microcarriers when cells were inoculated into a spinner vessel. Then, cells could continuously grow in serum free medium with having specific growth rate of 0.106 (1/day) and specific scu-PA production rate of
(IU/cell/day) in batch cultivation.
A Study on Continuous Alcohol Fermentation with Cell Recycle by Means of Membrane Separation
KSBB Journal, volume 7, issue 2, 1992, Pages 139~143
One of the objectives of this work is to obtain information relevant to the industrial production of alcohol from sugar. The fermentation of alcohol by a strain of saccharomycess cerevisiae ATCC 24858 was studied In a continuous single-stage process with recycle of the cells via tangential flow microfiltration membranes. The experimental results reported in this study pertain to continuous cultures with total cell-recycle by varying the dilution rate (D=0.3, 0.5, and 0.7
) and glucose concentration (50, 100, 150, and 200g/l sugar solution). Productivity using a repeated cell recycle system was found extremely high, 1.e., over 10 to 29 times higher than that of a smile batch system. When a sugar concentration of 200g/1 at dilution rate, 0.7 hr-1 was used, 83.9g/l ethanol was formed with an ethanol yield of 0.42(82% of theoretical) based on sugars utilized.
Production of Sorbitol Using Dried and Immobilized Zymomonas mobilis
KSBB Journal, volume 7, issue 2, 1992, Pages 144~148
The purpose of this study is to develop a continuous process for sorbitol production using dried Zymomonas mobilis immobilized in K-carrageenan. The methods of glularaldehyde cross-linking of enzymes in CTAB (celyltrimetylammoniumbromide) treated cells immobilized in K-carrageenan showed stability for the production of sorbitol for 30 days of operation. K-carrageenan beads entrapping permeabilized cells were dried to Improve bead rigidity and storage stability. A semi-batch process with dry beads was carried out and only a small loss of enzyme activity (less than 8%) was observed during 72h. The value of Vmax for the dry K-carrageenan beads was found to be one half or that for free cells. It was shown that the productivities of the continuous process with wet K-carrageenan beads and dry beads at a dilution rate 0.1h-1 were 3.4g/L-h and 2.88h/L-h, respectively.
Identification and Cultivation of Pseudomonas fluorescens Antagonistic to Pseudomonas tolaasii
KSBB Journal, volume 7, issue 2, 1992, Pages 149~153
Pseudomanas fluorescens was selected from mushroom and studied in both batch and continuous culture in order to find out optimum conditions for cultivation. P. fluorescens is an aerobic bacterium and antagonistic to Pseudomonas tolaasii which causes blotch disease on the mushroom cap. Cells of P. fluorescens were grown well on medium containing 30g/L of glucose, whereas the growth was inhibited with the glucose concentration at higher than 30g/L. The highest value of specific growth rate and productivity were obtained when using 10g/L of yeast extract. Optimum concentrations of
for culture were found to be 1.0g/L and 0.1g/L respectively. Optimum concentration of
used as a sulfur source was 1.0g/L. It was also found that the cell concentrations were at the maximum level when grown on the medium containing 1.0g/L of
and 0.1g/L of
. Also, the optimum culture conditions were
and pH 6.0. Cultivation of P.fluorescens at high initial dissolved oxygen (D.O) value led to a decrease of bacterial productivity in batch culture. Maximum productivity was achieved at 68 for the initial D.O value.