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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 7, Issue 4 - Dec 1992
Volume 7, Issue 3 - Sep 1992
Volume 7, Issue 2 - Jul 1992
Volume 7, Issue 1 - Apr 1992
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Plant Protein Improvement by Synthetic Gene
KSBB Journal, volume 7, issue 3, 1992, Pages 155~160
To improve the nutritional quality of plant proteins, a synthetic gene, called HEAAE (high essential amino acid encoding)-DNA, was introduced and expressed in tobacco plants. The synthetic gene, which is 292 basepair-long, codes for a protein composed of about 80% essential amino acids. To improve its expression level in plants, Cauliflower Mosaic Virus (CaMV) 355 and CaMV duplicate 35S promoters which are known as strong promoters were used with Nopaline Synthase promoter as a control. Transformed and regenerated tobacco plants were subject to analysis for introduction and expression of this gene. Integration of the gene into the plant genome and its expression into mRNAs and its proteins have been demonstrated using Southern, northern blot analysis and amino acid analysis. The differences of expression levels among CaMV duplicate 35S, CaMV 35S and Nopaline Synthase promoters are significant in term of mRNAs, but not in terms of proteins.
Synthesis of ADD and AD by Mycobacteria sp. in Microemulsions
KSBB Journal, volume 7, issue 3, 1992, Pages 161~165
Water-insoluble sitosterol was biotransformed to AD(4-androstene-3, 17-dione) and ADD(1, 4-androstadiene-3, 17-dione) with crosslinked Mycobacteria sp. NRRL B-3683 in microemulsions. The Mycobacteria activity depended on the kinds of surfactants and water content. The activity was very high in cationic microemulsion (CTAB-buthanol-cyclohexane-water) and five times higher than that of buffer, but it showed a very low activity in anionic microemulsion. The Mycobacteria activity was increased as increasing as increasing water contents and in the microemulsion containing 20% water was doubled in the same microumulsion containing 5% water. We suggest that microemulsion is effective reaction medium for Mycobacteria bioconversion.
The Production of Biopolymer by Zoogloea ramigera
KSBB Journal, volume 7, issue 3, 1992, Pages 166~171
Zoogloea ramigera 115 was cultured for biopolymer production and its bioflocculant usages. Cultural conditions of the organism were examined with regard to high production of the microbial polysaccharide. The most suitable medium was found to contain glucose as a carbon source,
as a nitrogen source, and yeast extract as an organic nutrient. The initial pH of 6.0 proved to optimal. The biopolymer was extracted effectively using ultrasonication and high speed centrifugation, followed by propanol addition. Jar test results indicate that the polysaccharide produced by the organism is an effective flocculant.
Immobilization of Rhizopus chinesis using Polyurethane Foams
KSBB Journal, volume 7, issue 3, 1992, Pages 172~178
A simple and effective method has been developed for the immobilization of lipase producing Rhizopus chinensis on polyurethane foam. In this method, the fungal cells with 1, 3 specific lipase in there inside are immobilized within the foam matrix. Four types of commercially available polyurethane foam were tested. The ultimate purpose of the process is to produce low-cost biocatalysts for lipase-catalyzed reactions, which are being increasingly used for industrial applications. Effects of several parameters were studied on the cell loading and the hydrolytic activity of intracellular lipase after acetone drying. These parameters were the type, size, and amount of polyurethane foam. In all the cases, the intracellular lipase activity obtained with the foam was approximately twice greater than that obtained in the absence of the foam.
Production of Sorbitol from Fructose in Charged Membrane Bioreactor With NAD-Regeneration System
KSBB Journal, volume 7, issue 3, 1992, Pages 179~185
An anion-charged membrane was used for selective retention of coenzyme NAD(H) in reactor without any chemical modification. The membrane could reject permeation of NAD (H) (80.9%) but not reject permeation of product. The retention ratio was enhanced in the presence of albumin and Tris-maleate buffer. A bioreactor equipped with a membrane, NTR 7410 was constructed and used in the repeated batch production of sorbitol. NADH-dependent sorbitol dehydrogenase from sheep liver was used for the production of sorbitol from fructose. The coenzyme oxidized was regenerated with alcohol dehydrogenase. 47g/L sorbitol was produced for 198 hr with a substrate conversion ratio of 70%. The retention ratio was almost maintained throughout the entire reaction.
Studies on the Compositions of Amino acid and Fatty acid of Pine caterpillar(Dendrolimus spectabilis)
KSBB Journal, volume 7, issue 3, 1992, Pages 186~190
The compositions of amino acid in the protein and total fatty acid of Dendrolimus spectabilis were analyzed quantitatively by HPLC and GC, respectively. The contents of crude oil and protein from the extracts were 21.00% and 58.47%, respectively. The amount of free amino acids in the protein was 3.65g/100g, and 1.31g/100g of essential amino acids was contained in the free amino acids. The amount of total amino acids in the protein was 41.20g/100g, and 14.75g/100g of essential amino acids were contained in the total amino acid. The compositions of fatty acid in the oil were
=5.21% in order, respectively. 45.88% of unsaturated fatty acids were contained in the oil.
Identification of Agrobacterium tumefaciens from Soil and Transformation of Maize
KSBB Journal, volume 7, issue 3, 1992, Pages 191~200
Several strains of Agrobacterium tumefaciens were isolated from soil in the Taegu area and characterized to develop some useful vector systems for higher plant genetic engineering. The selected colonies had a unique form, and strains from the colonies were capable of tumor formation on the sunflower leaf surface. They had a large plasmid. The restriction analysis showed that they were another kinds of Ti plasmic compared with C58 and Ach5. The isolated strains were identified as the nopaline type and also as biovar 1 A. tumefaciens, according to their tumor morphology, blophyslcal and biochemical characteristics. One of the isolated strains, AK204 was transformed with binary vector (pGA642), having selectable marker (Kmr, Tcr). Furthermore, maize tissue cells were transformed by cocultivation with AK204/pGA642, and the transformants were selected on the selective medium and identified using PAGE patterns of their soluble proteins.
Dependence of High Affinity Binding of Epidermal Growth Factor on Receptor Cytoplasmic Domain
KSBB Journal, volume 7, issue 3, 1992, Pages 201~208
Cell surface binding of epidermal growth factor(EGF) to EGF receptors was studied for a series of site-directed receptor mutants transfected into B82 mouse fibroblasts. Scatchard plots for truncation mutant receptors significantly lost nonlinearity for truncations below residue 1022. Transient plots of dissociation kinetics exhibited biphasic behavior for all receptor types, but the fraction of receptor in slow-dissociating form was reduced by an order of magnitude for the truncation mutants below residue 1022. Comparison of dissociation kinetics between control cells and cells treated with Triton X-100 revealed no significant variation for the slow-dissociating receptor form, but a noticeable variation was observed for the fast-dissociating receptor form when EGF receptors were truncated below residue 991. These results suggest that high affinity of EGF binding at cell surface depend on the EGF receptor cytoplasmic region.
Study on the Action Mechanism of Polycation in Cell Wall Formation of Suspension Cultured Cells of Daucus carota
KSBB Journal, volume 7, issue 3, 1992, Pages 209~215
The aims of this sutdy was to investgate the action mechanism of polycation on the
-glucan synthetase II (GS II) related to cell wall synthesis in suspension cultured carrot cells. In the suspension cultured cells treated with poly-L-Iysine(
) and poly-L-ornithine (
) having ploycationic nature, GS II activity increased about 40% and 50% than that of the control respectively. And similar response was observed when ATP and NaF were treated. On the other hand, ploy-L-lysine and ploy-L-ornithine did nor affect the membrane permeability. Phorbol-12-myrlstate-13-acetate (TPA), activator of protein klnase, increased about 35% and 1-(5-isoquinolinyl sulfonyl)-2-methyl-piperrazine (H-7), inhibitor of protein kinase, decreased about 30% of GSII activity than that of control. These results suggest that polycation plays a role in the cell wall synthesis by increasing GS II activity through phosphorylation.
Studies on the Isozyme Patterns Followed by Redifferentiation Stages of the Pepper(Capsicum annuum L.) - Changes of isozyme pattern on somatic embryogenesis in cutured pepper cells -
KSBB Journal, volume 7, issue 3, 1992, Pages 216~221
The physiological changes of somatic embryogenesis in cultured pepper cells (Capsicum annuum L. cv Shinhong) were investigated. The somatic embryogenesis was induced by cultivating the callus in hormone-free MS medium. The peroxidase patterns in the somatic embryogenic cells and the culture medium was revealed three and two of cathodic and anodic bands by isoelectric focusing respectively. Activity of peroxidase released into culture medium was 4 times higher than that of 12th day cultured cells. At the heart stage, the isozyme patterns of the MDH and esterase were found to be changed, which were showed by starch gel electrophoresis. It means these isozymes can be used as markers for studying somatic embryogenesis and differentiation.
Enhanced Berberine Production in Sucrose Solution by Plant Cell Suspension Cultures of Thalictrum rugosum
KSBB Journal, volume 7, issue 3, 1992, Pages 222~228
The effects of sucrose solution on cell growth and berberine production were studied in Thalictrum rugosum cell cultures. Application of sucrose solution without any other components enhanced berberine accumulation significantly in spite of slower cell growth. At high sucrose concentration, cells became very compact and fourfold increase in specific berberine content was achieved. Optimum concentration of sucrose in plain water to maximize the productivity was found to be 8%. Time course experiment in 8% sucrose solution showed that more than 5 days were required to utilize the advantage of this system efficiently. Addition of vitamins, growth regulators, and inorganic salts into the solution was not effective in increasing berberine productivity.
Direct Alcohol Fermentation of Starch by Schwanniomyces castellii
KSBB Journal, volume 7, issue 3, 1992, Pages 229~234
Alcohol fermentations were carried out to confirm the capacity of ethanol production from glucose, starch and soluble starch(dextrin) by Schwanniomyces castellii NRRL Y-2477. Schw. castellii NRRL Y-2477 was able to produce the 63.9g/l ethanol using 94% subtrate from 150g/l-glucose medium. The direct alcohol fermentation of starch having the maximum solubility of 20g/1 at
yielded 9.1g/l ethanol upon complete depletion of starch, whereas 34.5g/1 ethanol was produced by utilizing 82% of 100g/1 soluble starch medium. The fermentation of 150g/1 soluble starch produced 52.1g/l ethanol using about 79% of substrate. Thus, it was found that the limiting step of direct alcohol fermentation of starch by Schwanniomyces castellii NRRL Y-2477 was a hydrolysis of starch.
The Basic Design Parameters and Effluent Characteristics for Aerobic Fixed Biofilm Reactor
KSBB Journal, volume 7, issue 3, 1992, Pages 235~245
A number of experiments to treat wastewater of a dye plant were conducted to investigate the basic design parameters and effluent characteristics for aerobic fixed biofilm reactor upon the variation of organic loading rate. The media used for this study were SARAN 4000 D with specific surface area
, and 1000 D with specific surface area
. The influent COD concentration ranged from 1250 to 4080 mg/L. Substrate removal and variation of biomass concentration were observed. At the beginning, the effluent quality was poor but improved as the time passed. However the effluent quality became seriously deteriorated when the media clogged. At influent COD of 1250mg/L, the effluent COD varied little, even if the organic loading rate increased from 0.63 to 2.5kg COD/
/day. It was also noted that the design parameters for activated sludge process could be applied to an aerobic fixed biofilm process under the experimental conditions.