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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal Basic Information
Journal DOI :
Korean Society for Biotechnology and Bioengineering
Editor in Chief :
Volume & Issues
Volume 7, Issue 4 - Dec 1992
Volume 7, Issue 3 - Sep 1992
Volume 7, Issue 2 - Jul 1992
Volume 7, Issue 1 - Apr 1992
Selecting the target year
A Study on the Functional Relationship between Biomass Concentration and Fermentation Characteristics in the Culture of High density Cell
KSBB Journal, volume 7, issue 4, 1992, Pages 247~251
The effect of biomass concentration of Saccharomyces cerevisiae ATCC 24858 on specific growth rates, biomass yields, ethanol yields and productivity in the batch fermentation of rotary shaker was investigated. The specific growth rate decreased according to the increase in the biomass density and finally became zero at a biomass concentration, 55g/L. The ethanol yield
represented a constant value, 0.43, regardless of the change of biomass concentrations. However, the biomass yield
showed a trend to diminish in values with augmentation of biomass density and ultimately to reach zero at 55g/L of biomass concentration. The ethanol productivity increased linearly with biomass concentration so that, in case of initial sugar concentration, 170g/L, the productivity for 55g/L of biomass density rose up to 30g/L
hr for all the batch fermentations. And also the ethanol concentration inhibiting completely the growth was verified 95g/L by applying experimental data to Luong's equation.
Production of Citric Acid in a Flat-type Membrane Bioreactor
KSBB Journal, volume 7, issue 4, 1992, Pages 252~257
A flat-type membrane bioreactor(FMBR ) for aerobic whole cell immobilization was developed and its performance for the citric acid production was investigated using Aspergillus niger (KCTC 1232). The reactor consisted of three layers. The top layer contained flowing air for oxygen supply, the middle layer had stationary cells, and the bottom layer had flowing aqueous nutrients. The initial pH of the culture medium played an important role in citric acid production and the lower initial pH of the culture medium resulted in a higher citric acid yield. Under air and pure oxygen aerations the volumetric productivity reached 0.20 and 0.40g/Lh. Furthermore, the productivity improved with the increase of the culture medium feed rate.
Optimization of Cell Concentration and Dilution Rate in Cell Recycled Ethanol Fermentation
KSBB Journal, volume 7, issue 4, 1992, Pages 258~264
The rheological characteristics of the ethanol fermentation broth were pseudoplastic when the yeast concentration was above 150g/L. From the viewpoint of rheological properties, the cell concentration below 150g/L was recommended for ethanol fermentation. Since the cell floc was formed at the cell concentration of 100 g/L, yeast cells were not much plugged in the pores of the membrane. The cell concentration above 100g/L was desirable when considering the permeability of the membrane. Since ethanol productivity was the highest when the cell concentration was 130 g/L in cell recycled ethanol fermentation. The optimal dilution rate was determined at 1.3 h-1 at constant cell mass of 130g/L. At this dilution rate, the ethanol productivity and glucose conversion ratio ware 80 g/L
h and 0.94, respectively.
Growth model for Pichia stipitis growing on sugar mixtures
KSBB Journal, volume 7, issue 4, 1992, Pages 265~270
Low cost fermentation substrates frequently contain a mixture of carbon sources including hexoses, pentoses and disaccharides. Fermentation of such mixtures requires an understanding of how each of these substrates is utilized. During batch culture of Pichia stipitis CBS 5776 on sugar mixtures, glucose causes catabolite repression of xylose and cellobiose utilization. Also, glucose causes a permanent repression of xylose utilization as evidenced by reduced growth rates during the xylose phase of glucose/xylose fermentation. The growth model for multiple substrates is developed based on a cyclic AMP mediated catabolite repression mechanism and this model adequately described the growth and ethanol production from sugar mixtures.
Primary culture of adult rat hepatocytes and assay of hepatic functions
KSBB Journal, volume 7, issue 4, 1992, Pages 271~277
Rat hepatocytes were isolated by collagenase perfusion method and cultured on the collagen coated dish or on the floating collagen membrane. Using the primary cu1tured hepatocytes, the efficiency of cell attachment and the hepatic functions such as gluconeogenesis, ureogenesis and albumin synthesis were studied. The cell viability was kept above 50% until 5 days and the hepatic functions of ammonia metabolism and albumin synthesis were maintained until 7 days. Floating collagen membrane was found to be more efficient than the collagen coated dish for the maintenance of hepatic function in-vitro.
Effect of cell morphology on the hepatic functions adult rat hepatocytes
KSBB Journal, volume 7, issue 4, 1992, Pages 278~283
Rat hepatocytes were isolated and cultured on the petri dishes treated with various coating materials. Untreated or collagen coated petri dish gave monolayer culture of hepatocyte and proteoglycan, dermatan sulfate, and BSA treated petri dish gave hemispheroid. The untreated Primaria petri dish gave spheroid type of hepatocyte, and heparin and hyaluronic acid treatment gave multilayers. To sustain high cell viability, monolayer cultured hepatocytes was more useful, while it was found that the hemispheroid or spheroid type hepatocytes was more active in the hepatic functions such as ammonia metabolism and albumin synthesis.
Production of L-tryptophan by Genetically Engineered Klebsiella pneumoniae
KSBB Journal, volume 7, issue 4, 1992, Pages 284~289
The optimum conditions for the production of tryptophan using a recombinant Klebsiella pnuemoniae phe A tyr A trp R/pSC 101-
and its plasmid stability during tryptophan production were studied. The optimum temperature was
and the specific growth rate was 1.05
. Tryptophan production was increased by glucose fed-batch culture, and tryptophan was accumulated to 0.175 g/l after 36 hrs. This amount was about 1.2 and 1.6 times greater than that obtained from batch culture and flask culture, respectively. The stability of the strain in fed-batch cu1ture was greatly different from that in repeated flask culture. After 6 generation, 95% of total cells was stable in repeated flash culture, but in fed-batch culture only 50% was stable.
Effect of Lactobacilli on Reactive Oxygen Scavenging and Immune Stimulation
KSBB Journal, volume 7, issue 4, 1992, Pages 290~295
Reactive oxygen scavenging activity and immune stimulatory activity of lactobacilli were investigated by different free radical scavenging assays and Ig G assay. Lactobacilli culture (S/N) and its complex with
have significant effects in XOD assay and response to paraquat. Cell free extract significantly prevented the photohemolysis. Thus, it seems that each sample from lactobacilli has a different free radical scavening mechanism. Furthermore, it is assumed that cell free extract from lactobacilli activates antibody stimulation of B cell through a stimulation of T cell.
Identification of Pseudomonas fluorescens antagonistic to Pseudomonas tolaasii and its cultivation
KSBB Journal, volume 7, issue 4, 1992, Pages 296~301
A Pseudomonas fluorescens was selected from mushrooms and studied in both batch and fed-batch cultures in order to get maximal biomass concentration. P. fluorescens is an aerobic bacterium and antagonistic to Pseudomonas tolaasii which causes blotch disease on the mushroom cap. P fluarescens and P. tolaasii were identified by Gram staining, gelatin liquefaction, oxidase test, etc. and were characterized by pigment production, temperature sensitivity, salt tolerance and rapid pitting test, etc., Celts of P. fluorescens well in medium containing 30g/L of glucose, whereas the growth was inhibited at the glucose levels at higher than 30g/L. The highest values of specific growth rate and productivity were obtained when using 10g/1 of yeast extract. Optimum concentrations of
for culture were found to be 1.0g/L and 0.1g/L respectively. Optimum concentration of
used as a sulfursource was 1.0g/L. It was also found that the cell concentrations reached the maximum level when grown on the medium containing 1.0g/L of
and 0.1g/L of
. Also, the optimum culture conditions were
and pH 6.0. Cultivation of P. fluarescens at high dissolved oxygen (DO) concentration led to a decrease of bacterial productivity in batch culture. Maximum productivity was achieved at 40% DO concentration.
Inhibitory Effect of Lipid Bilayer Membrane on Protein Phosphatase 2A
KSBB Journal, volume 7, issue 4, 1992, Pages 302~307
Protein phosphatase 2A was obtained from a cytosolic fraction of bovine brain homogenate. The phosphatase activity using phosphorylated histone Hl as substrate was suppressed in the presence of liposomes composed of dipalmitoylphosphatidylcholine(DPPC) or the mixture of phosphatidylserine and DPPC. The binding of protein phosphatase to liposome was indicated by the facts that the phosphatase activity of the supernatant of protein phosphatase/multilayer vesicle mixture was decreased with increasing amount of liposome, and that [
]-labeled protein phosphatase was coeluted with liposome. However, the affinity of the protein for phospholipid membrane was not so high. On the other hand, okadaic acid and liposome reduced the phosphatase activity synergistically, which means that okadaic acid binds neither to lipid membrane nor to the membrane-associated phosphatase, The inhibitory effect of liposome was, therefore, ascribed to association of the protein phosphatase 2A with the lipid bilayer membrane.
Analysis of two-stage Continuous Culture System by Transient Response of Single-stage Continuous Culture System
KSBB Journal, volume 7, issue 4, 1992, Pages 308~316
Two-stage continuous culture system has been studied intensively to maximize the productivity of a cloned gene product in unstable recombinant microorganism. As an effort to optimize the two-stage process, transient behavior of the second-stage was studied theoretically as well as experimentally using Escherichia coli Kl2
trp. A mathematical model describing the transient response to a step change in dilution rate was developed based on the assumption that the adaptation rate of cell growth is proportional to the available growth potential, which is defined as the difference in dilution rates between before and after shift-up. The kinetic parameters appearing in the model equations were the dimensionless step increase in growth rate(
) and the adaptation rate constant(k). These parameters were evaluated for various dilution rates and temperatures by washout method. This relatively simple adaptation model could predict the specific growth rate of the second-stage successfully. Advantage and disadvantage of the proposed model are also discussed.
The Effects of Surface Functional Groups to Protein Adsorption
KSBB Journal, volume 7, issue 4, 1992, Pages 318~324
The adsorption characteristics of bovine serum albumin(BSA) on the modified carbon fiber and cellulose surfaces were investigated. In order to define the effects of solid surface characteristics on protein adsorption, surfaces of carbon fiber and cellulose were modified by physical and chemical treatment. The amounts of BSA adsorbed onto various solid surfaces were evaluated by batch method under various pH and ionic strength. The amount of adsorbed BSA was highly dependent on pH as well as surface functional groups.