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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 8, Issue 5 - Dec 1993
Volume 8, Issue 4 - Dec 1993
Volume 8, Issue 3 - Sep 1993
Volume 8, Issue 2 - Jun 1993
Volume 8, Issue 1 - Mar 1993
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Cultural conditions and growth characteristics of indigo (Polygonum tinctorium) cells in an air-lift bioreactor
KSBB Journal, volume 8, issue 3, 1993, Pages 193~199
To find out the optimum conditions for indigo cell culture in air-lift bioreactor, effects of media composition including nutrients and precursors of the indigo colorants on the cell growth and characteristics of the cell growth under various cultural conditions were analyzed. Optimum cultural conditions were tested and the growth characteristics were analyzed in external and internal loop type air-lift bioreactors during 14-day culture. Better cell growth was obtained when the inoculum size was higher in the range of 0.5∼2.5% packed cell volume tested. In the sucrose concentration of 2 to 4%, the cell growth was better when the sucrose concentration was 4% (w/w) in both types of reactors. Sucrose was used up in the early stage of exponential phase of growth At the optimum concentration of a Precursor tryptophan at 1 U UW was 3.8 g/l in internal loop bioreactor, and 3.5 g/l in external one after 14 days of cultivation. Addition of indole showed negative effect on cell growth of suspension culture in air-lift biorector culture and cell mass of 2.5 g/l and 2.2 g/l were obtained in external and internal loop bioreactor, respectively. Selected inorganic nitrogen source potassium nitrate showed about 110% increase in cell growth than that of control. DCW was 16.34 g/l under optimum conditions during 14-day cultivation in internal loop bioreactor.
A Study of Alginic acid Membrane for the Separation of Water-Alcohol Mixtures
KSBB Journal, volume 8, issue 3, 1993, Pages 200~208
As a separation membrane for water-ethanol mixtures, alginic acid has been used which is hydrophilic polysaccharide and has excellent bonding capacity with divalent metal ions. Pervaporation characteristics of the alginic acid membrane were examined. The membrane was crosslinked with metal ions for the improvement of mechanical strength and chemical affinity. And its pervaporation characteristics were investigated. The first group(I A) metal complexed membrane cannot be used because of their brittleness and excessive swelling in low concentrations of ethanol solution. But the permeation characteristics of other metal complexed membrane were more improved than that of the alginic acid membrane because of their contraction of the membrane and hydrophilic property of metal ion.
Isolation of the Biodegradable Peptide Polymer-Producing Bacterial Strain and Characterization of the Polymer Produced by This Strain
KSBB Journal, volume 8, issue 3, 1993, Pages 209~216
For the production of biodegradable polymers from microorganisms a bacterial strain producing a biopolymer was isolated from soil. The bacteriological characteristics of this strain and physicochemical Properties of the biopolymer produced were investigated. The bacterial strain was identified as an alkalophilic Alcaligenes sp. The Purified biopolymer treated with cetylpridinium chloride and acetone was identified as an acidic biopolymer having carboxyl groups and showed strong UV absorbance (at 210nm). The biopolymer was composed of 100% glutamic acid and glutamic acid existed as
-PGA) in the form of the
-peptide bond. The equivalent weight of this
-PGA was estimated about 350, indicating that one acidic fraction per 2.7 residue of
-polyglutamic acid existed. The molecular weight was
-PGA Production by Cultures of Alkalophilic Alcaligenes sp.
KSBB Journal, volume 8, issue 3, 1993, Pages 217~223
Methods for production of
-PGA ) by an alkalophilic Alcaligenes sP. were investigated for batch and continuous culture processes. Both quantity and productivity of
-PGA by Alcaligenes sp. in batch culture were gradually increased with the increase of glucose concentration up to 50g/l , but the maximal production yield of 63% was obtained at 10g/l of glucose concentration. The highest specific growth rate was about
at 50un of glucose concentration, and substrate inhibition was observed at above 50g1f of glucose concentration. The highest
-PGA formation about 11g/l in a batch system was obtained at 31'C, pH 10.0 and 87rpn Productivity of 2.80g/l/hr for continuous cultivation was 9 times higher than the productivity for batch cultivation.
Effects of Rosa rwgosa Radix on Lipid Metabolism in Rats
KSBB Journal, volume 8, issue 3, 1993, Pages 224~229
The feeding effects of Rosa rugosa roots powder extracted with two different extracting solvents on lipid metabolism in rats were evaluated. Rats were fed with purified diets containing methanol or 70% aqueous acetone extracts at a 1% level for 4 weeks. In addition, to examine the effects of age, rats with different ages (4weeks old vs. 3 months old) were fed with purified diets containing methanol extracts at a 1% level for 4weeks. Feeding of methanol extracts decreased the concentration of triacylglycerol in serum and liver, whereas the aqueous acetone extracts lowered the concentration of liver triacylglycerol with concomitant elevation of the concentration of serum triacylglycerol. The methanol extracts ameliorated the degree of increase in the serum triacylglycerol level in relation to age and decreased especially the concentration of free fatty acid in serum of adult rats. The results suggested possibility that the regulatory mechanism responsible for triacylglycerol-lowering effects of Rosa rugosa roots extracts in the liver could be modulated by differences in the components of extracts due to the variation in extracting solvents, and that the methanol extracts might be effective in lowering an age-dependent increase in the concentration of serum and liver triacylglycerol in rats.
Development of New Biodegradable CHITULOSE film from Composite of Chitin and Cellulose
KSBB Journal, volume 8, issue 3, 1993, Pages 230~236
A Chitulose film was made by dissolving chitin and cellulose in a dimethylacetamide/LiCl solvent system This film was completely degraded in 20 days in soil and penetrated by soil microorganisms in 16-26 hours. A permeability analysis of the film showed that water permeability was in the range of 0-187.5
/day, depending upon the ratio of chitin to cellulose and decreased with increasing a cellulose content in the Chitulose film The permeabilities of organic compounds,
and oxygen change with the composition of the Chitulose file The film was tested with a burned 2~3degree rat for exploring its use as artificial skin. The rat was completely cured within 31 days without inflammation.
Development of New Biocompatible Fiber form Composite of Chitin and Cellulose
KSBB Journal, volume 8, issue 3, 1993, Pages 237~242
Chitin-based fibers have low mechanical strength and hence cannot be used as surgery fiber due to fast degradation In tissues. A new fiber Chitulose was made by mixing chitin with cellulose, both of which have similar structure. A mixture of dimethylacetamide (DMAc) and 6% lithium chloride (LiCl) was found to be an effective solvent system for dissolvoing chitin and cellulose. The Chitulose fiber made by wet spinning of a mixture of chitin and cellulose resulted in the highest degree of strength and flexibility when the ratio of chitin to cellulose was 1.5; 0.2. The fiber maintained mechanical structure even after autoclaving, indicating thermal stability. A biodegradability test of the Chitulose fiber by imbeding in a rat showed that degradation was initiated in 14 days and completely done in 40 days.
Development of Bioreactors for Hydrogen-Producing Immobilized Photosynthetic Bacteria(I) : Evaluation of lmmobilized CSTR for Hydrogen Productivity and Effectiveness Factor
KSBB Journal, volume 8, issue 3, 1993, Pages 243~255
In this study, it was observed that hydrogen Productivity varied with stirrer speed, bead radius, input glucose concentration and dilution rate in a continuous stirred tank reactor in which immobilized R. rubrum KS-301 was used as a hydrogen-producing bacterium The mass transfer resistance due to cell immobilization was also studied. In order to estimate an effectiveness factor, Des of glucose was first obtained, which was subsequently represented by the correlation equation between Dos and Xb, As a result external mass transfer resistance could be neglected for stirrer speeds greater than 400rpn With bead radius increasing, the hydrogen productivity and internal effectiveness factor decreased. With input 91ucose concentration increasing, the hydrogen productivity and interval and external effectiveness factor increased. Although an Internal effectiveness factor was not affected, hydrogen productivity Increased with dilution rate increasing. An overall effectiveness factor remained nearly constant for the dilution rates investigate4 but increased with input 91ucose concentration increasing.
Development of Bioreactors for Hydrogen-Producing Immobilized Photosynthetic Bacteria(II) : Evaluation of Immobilized Bioreactor for Hydrogen Productivity and Mass Transfer Resistance
KSBB Journal, volume 8, issue 3, 1993, Pages 256~265
In this study, it was observed that hydrogen productivity varied with changes of input g1ucose concentration and dilution rate in FBR( Fixed Bed Reactor), and CSTR(Continuous Stirred Tank Reactor). We evaluated and compared reaction rate Parameters and internal external and overall mass transfer resistances of immobilized carrier in both reactors. Apparent
decreased with increasing dilution rate in FBR but showed a constant value above
of dilution rate in CSTR. The experimental results in FBR showed nearly analogous to those in CSTR, however, the performance of FBR resulted in lower hydrogen productivity and an external effectiveness factor but a higher internal effectiveness factor than in CSTR. The overall effectiveness factor obtained with various input 91ucose concentrations showed similar values in both reactors.
Modeling the effect of mass transfer on the kinetics of fructo-oligosaccharide production by immobilized cells
KSBB Journal, volume 8, issue 3, 1993, Pages 266~271
The effective diffusion coefficients of glucose, sucrose and fructo-oligosaccharides in Ca-alginate gel beads at high concentration of sucrose solutions were investigated at
. A mathematical model for the kinetics of fructo-oligosaccharide production using immobilized cells was proposed and compared with experimental results varying the bead size, the substrate concentration and the bead ratio. Very low values of diffusion coefficients ranging
/sec were obtained, and the predicted results were in good agreement with experimental ones in all cases tested.
Estimation of Fermentation State and Metabolic Stoichiometry of Kyuywomyces marxianus
KSBB Journal, volume 8, issue 3, 1993, Pages 272~281
State varibles were estimated for fermentations of K. marxianus under various dilution rates and dissolved oxygen concentrations. The number of elementary reaction stoichiometry with fixed coefficients was determined by singular variable decomposition. Stoichiometry with feasible physical meaning was obtained by target factor analysis. States of fermentations were estimated by linear quadratic programming. The process conditions of single cell production to maximize carbon source consumption were suggested.
Foam Separation of Yeast Cells
KSBB Journal, volume 8, issue 3, 1993, Pages 282~286
A bath foam separator has been used to separate yeast cells from their culture broth without the use of any surface-active agents. The cell separation ratio was improved in proportion to a separator level and nitrogen flow rates. Percentage of yeast removal after 3 minutes of operation was more than 90. The cell separation ratio was also improved by the addition of 0.2g/l
An Integrated Process for the Separation and Purification of Biologically Active Proteins from Human Urine
KSBB Journal, volume 8, issue 3, 1993, Pages 287~294
For the purpose of combining the purification processes for several biologically active proteins form human urine, an efficient integrated fractionation procedure has been investigated. The procedure was started by concentration with ultrafiltration and pH precipitation followed by a selectable combination of chromatography on gel filtration, adsorption, ion exchanger, affinity, and reverse phase column. By this process, the purified urokinase, epidermal growth factor and albumin migrated as a single band on SDS-polyacrylamide gel electrophoresis and were fully active. The recoveries of these purified proteins were 48%, 17%, and 46%, respectively.
Effect of Fungal Elicitor, Pluronic F-68 and Methylcellulose on Suspension Culture of Mentha piperita Cells
KSBB Journal, volume 8, issue 3, 1993, Pages 295~299
The effect of fungal elicitor, Pluronic F-68 and methylcellulose on suspension culture of M piperita cells was investigated in shake flasks. About a two-fold increase in oil production was observed in response to the treatment of the fungal elicitor prepared from Rhodotorula rubra. Low concentration of Pluronic F-68 or methylcellulose enhanced Peppermint cell growth at 100 rpm of agitation.
Brassinosteroid Substances in Immature Zea mays Seeds
KSBB Journal, volume 8, issue 3, 1993, Pages 300~305
In order to explore the brassinosteroid-active components in Zea mays seeds, the methanol extract was purified by the sequences of solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 chromatography, charcoal adsorption chromatography and Bondesil chromatography. The activity of brassinosteroid was monitored by the rice inclination test and its presence could be confirmed in each purification step. The purified active components were separated by silica gel adsorption chromatography. Brassinosteroid substances in separated active fractions were identified as castasterone and teasterone by HPLC. The content of brassinosteroid in Zea mays seeds as converted into brassinolide was 3-8ng/g fresh weight.