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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 8, Issue 5 - Dec 1993
Volume 8, Issue 4 - Dec 1993
Volume 8, Issue 3 - Sep 1993
Volume 8, Issue 2 - Jun 1993
Volume 8, Issue 1 - Mar 1993
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Production of Fructo-oligosaccharides by the Fructosyltransferase Immobilized onto an lon-exchange Resin
KSBB Journal, volume 8, issue 4, 1993, Pages 307~312
A fructosyltransferase from Aureobasidium pullulans was immobilized onto a polystyrene-type anionic ion-exchange resin and the production of fructo-oligosaccharides was Investigated by the immobilized enzyme. The optimum pH and the temperature of immobilized enzyme were found to be pH 5.0,
respectively. The thermal stability of the enzyme was greatly enhanced after immobilization. The reaction profiles of the immobilized enzyme was almost identical to those of the free cells and the soluble enzyme. The immobilized enzymes were stable up to 20 cycles without loss of initial activity in a repeated-batch operation
Enzymatic Synthesis of Meth.yl Fructoside by Immobilized Invertase
KSBB Journal, volume 8, issue 4, 1993, Pages 313~319
Methyl fructoside was synthesized from sucrose and methanol using an immobilized invertase. The enzyme was covalently bound by glutaraldehyde on porous silica coated with polyethyleneimine to give loading capacity of 120mg of invertase per one gram of dry porous silica and effective activity of 100U per one milligram of bound invertase. Polyethyleneimine coating imparted a hydrophillic character, good activity retention and high loading capacity to the surface of porous silica as well as hydrophillic microenviroment in the vicinity of bound invertase. The immobilized enzyme was formed into an alginate-enclosed silica bead to have enough activity for methyl fructoside synthesis from aqueous methanol-sucrose solution. Using the alginate-enclosed biocatalyst the yield of methyl fructoside was obtained as high as 55.9% from aqueous 30% (v/v) methanol and 0.291mo1/l sucrose with 2U/ml activity at
, pH 4.8.
A Study on Glycoside Synthesis Using Alginate-enclosed Microspheres
KSBB Journal, volume 8, issue 4, 1993, Pages 320~327
Latex microspheres of styrene/acryl copolymer with acrylamide functional group were used for the stable covalent immobilization of an enzyme applicable for enzymatic synthesis of glycoside. The latex microspheres were coated with polyethyleneimine to establish structural and functional properties relevant to the covalent Immobilization with a high retention of activity. Polythyleneimine-coated microspheres satisfactorily immobilized the invertase for methyl fructoside synthesis, and model reaction were formed into alginate-enclosed microspheres biocatalyst. Using the alginate-enclosed microspheres biocatalyst, the yield of model glycoside was obtained as high as 52.2% at concentration of aqueous 30%(v/v) methanol and 0.291mo1/1 sucrose solution with 2U/ml of activity. The present study showed that the latex microspheres were successfully applied to enzymatic synthesis of glycoside.
Development of Flow Injection Analysis System for Amperometric Determination of Cholesterol Using Immobilized Enzyme Columns
KSBB Journal, volume 8, issue 4, 1993, Pages 328~335
A flow injection analysis(FIA) system was developed for the determination of cholesterol using immobilized cholesterol oxidase and cholesterol ester hydrolase. The enzymes were immobilized on controlled pore galas(CPG) by the glutaraldehyde method. The glass colunms packed with immobilized enzymes were found to contain 3-5 I.U. for each enzyme. A hydrogen peroxide sensitive electrode was contructed and applied to the FIA system. The operational conditions for FIA response were investigated and optimized with variation of sampling volume, flow rate and composition of carrier solution. The FIA response were linear upto 60 and 400mg/m1 for free cholesterol and cholesterol ester, respectively. All samples were analyzed with a good precision (<2.5% CV) and accuracy. 23 samples were mea sured succesively within about an hour. Intermittent assays of more than 500 times caused 50% decrease in response sensitivity.
Removal/Recovery of Heavy Metals Using Biopolymer
KSBB Journal, volume 8, issue 4, 1993, Pages 336~340
Zoogloea ramigera 115, well known type of bacteria to produce slime in sewage plants, was selected for biopolymer production. The extracted biopolymer showed high uptake capacity of metals such as cadmium and zinc. Especially the fermentor broth itself showed high adsorption of metal and could be used a biosorbent without an additional separation process. Biopolymer was immobilized into beads of calcium alginate and used in a packed bed reactor for the purpose of valued metals recovery. The biopolymer showed high removal efficiencies of 80% or greater for Cu, Cd, Mn and Zn, and high stability in sorption-desorption-resorption experiments. The immobilized biopolymer systems were found to be comparable to other metal removal systems such as ion exchange resins and to be of potential industrial application value.
Production of Methanol by Resting Cells of Methylosinus trichosporium OB3b
KSBB Journal, volume 8, issue 4, 1993, Pages 341~350
As an effort to develop an alternative transportation fuel, the production of methanol from methane gas was studied using the resting cells of an obligatory methanotroph, Methylosinus trichosporium OB3b. The reaction was carried out in high concentration phosphate buffer solutions with the flask-grown cells containing the exclusively cytoplasmic methane monooxygenase (sMMO) activity. The methanol accumulation rate was observed to be 79nmo1/mg·min during the initial 4.5hr. Phosphate-dependent inhibition was found for both sMMO and methanol dehydrogenase (MDH) activities, and the inhibition constants were 185mM and 42mM, respectively. The inhibition mode was noncompetitive. Methanol was found to be very inhibitory to the sMMO activity and the inhibition constant (noncompetitive) was 21mM when propylene was used as substrate. The sMO activity in the resting cells was declined very fast and the rate became very high during the methanol production. These results indicate that the use of M. trichosporium OB3b as a biocatalyst for the methanol production is heavily dependent on the stable maintenance of the whole-cell SMO activity as well as the effective alleviation of product inhibition.
Comparison of ImmDbilization Techniques Using Phanerochaete chrysosporium for the Treatment of Pulp Waste Effluent
KSBB Journal, volume 8, issue 4, 1993, Pages 351~357
Three immobilization techniques and free cell system were tested to determine the most effective technique for the treatment of pulp waste effluent. The tests were conducted using Phanerochaete chrysosporium as a biocatalyst in a process designed to treat pulp waste effluent. The results show that Ca-alginate gel was the best immobilization material. The chosen material improved the stability and increased the removal efficiency of the system. The experiment using the chosen material was mom- bored for 400 hours with no significant changes in the state of the fungus. Common problems with other immobilization materials and free cell system were oxygen transfer resistance caused by air channelling and clogging in the bioreactor.
Effect of Ultrasound on the Acid Hydrolysis of Inulin
KSBB Journal, volume 8, issue 4, 1993, Pages 358~363
The effect of ultrasound on the acid hydrolysis of inulin was studied under significantly mild reaction conditions, at which sugar degradation products were not detected. Reaction conditions were i the range of 50~
and 0.1~0.3%(w/w) of HCl concentrations. The effects of reactor position inside water bath and mechanical agitation under ultrasound were investigated. The production rates of fructose with/without ultrasound irradiation were compared. The activation energies for both control and ultrasound reaction were the same, i. e., 25kca1/mo1, and ultrasound enhancement was average 22%.
Studies on the Biodegradation Test Method of Sufactant
KSBB Journal, volume 8, issue 4, 1993, Pages 364~369
The biodegradability of some anionic surfactants were investigated using biological oxygen consumption measurement at different temperatures. As test surfactants, soap, alkyl sulfate (AS), alpha olefin sulfonate (AOS), alkyl polyoxyethylene sulfate (AES), linear alkylbezene sulfonate(LAS), microbial surfactants such as sophorose lipid (sopholipid) and spiculisporic acid (S-acid), were used. The test solution were incubated at
, respectively. The comparative rates of biodegradation were in accordance with the results obtained from the surface tension measurement and methylene blue method. The results of comparative blodegradabilities of the surfactants were as follows; soap, AS>AES>AOS>LAS at
. However, at
, the biodegradation rate of soap was better than other surfactants. Considering the actual environment of the river, it was concluded that the biological oxygen consumption rate method at lower temperature was more practical than the current method such as methylene blue assay with adapted shaking flask culture at
Transported Metal ton by Crown Ether through Liquid Membrane
KSBB Journal, volume 8, issue 4, 1993, Pages 370~374
In transportation the amount of metal ion by crown ethers, dibenzo-18-crown-6 were investigated using ultraviolet spectrometer. Transported the amount of one valence metal ion as
was not so much. On the other hand, two valence metal ion increased by dibenzo-18-crown-6, which means that the ionic charge and hydration of two valence metal ion affected the carrying ability of crown ethers. The carrying ability of dibenzo-18-crown-6 was, therefore, adequate for two valence metal ion as
. It was also suggested that transport metal ion by crown ethers, which is related rather the catching ability than the selectivity of metalion.
Isolation and Cultivation of Microorganism Producing Lipoxygenase Inhibitor
KSBB Journal, volume 8, issue 4, 1993, Pages 375~381
The microorganisms producing a lipoxygenase inhibitor were screened from a wide variety of sources. The isolated strain was assigned to genus Penicillium by its cultural and morphological characteristics. The proper medium for the production of lipoxygenase inhibitor was composed of glucose 3.0%, ammonium sulfate 0.4%, and potassium phosphate (dibasic) 0.1%. The cultivation for lipoxygenase inhibitor production was carried out in 500m1 Erlenmyer flasks containing 100m1 of the medium at
by cultivating reciprocally. The highest lipoxygenase inhibitor production was observed after 8 days of cultivation. The inhibitor was the low molecular weight substance and inhibited specifically soybean origin lipoxygenase.
A Study of Organic Acid Formation and Dialysis Culture in E. coli Fermentation
KSBB Journal, volume 8, issue 4, 1993, Pages 382~389
In order to elucidate the effect of acetate ion on the growth of Escherichia coli, flask and fermentor cultures were performed using M9 and LB media. Acetic acid was secreted at a higher rate under the conditions of high glucose concentration as well as of richer medium, i. e., LB broth. The pH in flask culture could not be controlled as i fermentor and pH decreased with the formation of acetic acid. The inhibition effect of acetic acid was pronounced at a lower pH, and the effective inhibitory concentrations of acetic acid were 2.0g/l for LB flask culture, 4.0g/l for M9 flask culture, and 8.0g/l for M9 fermentor culture. Dialysis flask culture was designed to slowly provide E coli cells with glucose. Solid LB agar was layered under LB liquid medium with the variation of agar concentration and solid volume, The increase in the solid portion in the total volume(agar＋liquid) resulted in the increase of the final cell concentration. This can be ascribed to the fact that the larger solid phase behaves like a larger reservoir for glucose and controls the growth of E. coli with a controlled rate.
Effects of Various Elicitors on the Production of Berberine in Plant Cell Suspension Cultures of Thalicrtrum rugosum
KSBB Journal, volume 8, issue 4, 1993, Pages 390~396
Effects of various elictitors were investigated to enhance the production of berberine in plant cell suspension cultures of Thalicrtrum rugosum. Treatments of yeast elicitor, 15 different types of abiotic elicitors, 16 kinds of fungal elicitors from three species of fungi were performed. Cell growth and berberine production were examined and compared for both normal and elicitor treated cultures. No distinguished increases in berberine yield by the addition of elicitors could be attained.
Some Properties of Acetobacter sp. Isolated from Traditional Fermented Vinegar
KSBB Journal, volume 8, issue 4, 1993, Pages 397~404
Two strains were isolated from the vinegar of Korean traditional fermented rice wine and the vine gar of fermented persimmon, respectively. These strains, designated as KM and BPV, were identified as the genus Acetobacter with respect to morphological, physiological, and biochemical characteristics. The Isolates oxidized ethanol to acetate and over-oxidized acetate or lactate to CO2 and H2O. They were positive in catalase test, while being negative in oxidase, gelatin liquefaction, VP test, H2O production and indole formation tests. No
-pyrones ware produced from glucose and fructose. KM was tolerant of 11% ethanol while BPV was relatively sensitive to ethanol at a higher concentration than 5%. The guanine-plus-cytosine contents of the DNA of KM and BPV strains were 53.8 and 56.6 mol%, respectively. The cellular fatty acid compositions contained in these isolates were saturated straightchain C14:0 and C16:0,, and unsaturated straight-chain C18:1. Major ubiquinone system of KM was Q-9, but that of BPV was Q-10. In morphophysiological and biochemical aspects, KM strain was similar to Acetobacter pasteurianus. However, BPV strain was different from other Acetobacter type strains.
Multiple Forms of Serine-type Carboxypeptidase Produced by Absidia zychae
KSBB Journal, volume 8, issue 4, 1993, Pages 405~408
Absidia zychae NRIC 1199 produced two forms of carboxypeptidase(CPZ-1 and CPZ-2) which were distinguished in their isoelectric points but had almost identical properties(1). The amino acid sequences for the N-terminal of both enzymes were the same (Tyr-Thr-Ser-Pro-Lys-Leu-Xaa-Asp-Pro-Asp-Val) and any significant difference was not observed between amino acid compositions of the two enzymes. The ouchterlony double diffusion technique using antibody raised against the CPZ-2 protein demonstrated a good cross-reaction between CPZ-1 and CPZ-2 Genomic Southern analysis showed only one gene encoding CPZ in the genome of Absidia zychae. However, a significant difference between two enzymes was observed on peptide map using Staphylococcus aureus V8 protease, distinguishable only one band, indicating that multiple forms of CPZ are caused by post-translational modification, such as deamidation.
-Glucosidase from Aspergillus niger
KSBB Journal, volume 8, issue 4, 1993, Pages 409~414
This study was designed to reveal the conditions for
-glucosidase production from Aspergillus niger. The maximal enzyme production was obtained when the fungus was cultured at
for 5~6 days in the optimal medium containing 0.8% CMC, 0.5% beef extract, 0.3% Ca(NO3)2, 0.03% K2HPO4, 0.03% FeSO4, 0.05% Li2SO4, 0.2% tween 80, trace solution 1.0ml and initial pH 4.0, and then final enzyme activity under above conditions was 8.5-9.8 unit/ml culture filtrate.