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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 9, Issue 5 - Dec 1994
Volume 9, Issue 4 - Oct 1994
Volume 9, Issue 3 - Sep 1994
Volume 9, Issue 2 - Jun 1994
Volume 9, Issue 1 - Mar 1994
Selecting the target year
KSBB Journal, volume 9, issue 4, 1994, Pages 347~364
Airlift bioreactors are extensively used in the fields of aerobic fermentation, animal and plant cell cultures. This review article evaluates the present research activities in the field of airlift bioreactors. The published research works on the design parameters such as types, location and properties of gas sparser, hydrodynamic properties such as phase holdups mixing, liquid circulation rate, mass and heat transfer rates are summarized. Also, recommendations are presented for designing airlift bioreactors.
Effect of Ratios Carbon Source to Nitrogen Source on the Yields of PHB Fermentation Variables
KSBB Journal, volume 9, issue 4, 1994, Pages 365~371
The effects of ratios of initial concentration of carbon source to the initial concentration of nitrogen source in the fermentation media on both the yields of PHB fermentation variables and the accumulation of poly-
-hydroxybutyric acid(PHB) were investigated. The fermentation media were composed of the combination of varing glucose concentrations, 10, 20, 25, 30, 40,
and the NH4Cl concentrations 0.33, 0.4, 0.5, 1.5, 3,
. The yield of biomass on glucos, Yx/s, decreased very slowly according to the increase of the ratio of C to N. And the yield became constant at 0.35(g biomass/g glucose) with the ratio higher than 70. The yield of residual biomass, Yx/s, also decreased with the ratio of C to N and finally showed a constant value of 0.065(g residual biomass/g glucose) when the ratio was higher than 65. In accordance with the augmentation of the ratio, the yield of PHB, YPHB/S, however, increased and showed the maximum value of 0.35 (g PHB/g glucose) between 40 and 60 of the ratio. The maximum yield of PHB to the change of biomass, YPHB/S, was 0.87(g PHB/g biomass), and the yie1d YPHB/RX, was 4.2(g PHB/g residual biomass). The maximum accumulation percent of PHB to the final biomass was 81% when the ratio was higher than 67.
L-Lysine Production by 6-Azauracil Resistant Mutant of Corynebacterium glutamicum
KSBB Journal, volume 9, issue 4, 1994, Pages 372~377
To improve L-lysine yield, pyrimidine base analogue(6-azauracil)-resistant mutants were isolated from Corynebacterium glutamicum KFCC10672 Among them the best producer, C. glutamicum CH0516, was selected and tested for L-lysine production in a
fermentor. It was found that the product yield obtained with C. glutamicum CH0516 was higher than that of the parent strain by 3%. In order to elucidate the gain in productivity with the 6-azauracil-resistant mutant enzymatic kinetic parameters such as aspartokinase(AKase) and aspartate carbamoyltransferase (ATCase) were measured. The Km values of AKase with C. glutamicum KFCC10672 and CH0516 were 200.0 mM and 166.7 mM and those of ATCase were 0.13 mM and 0.27 mM, respectively. However, the specific enzyme activities of AKase of C. glutamlcum KFCC10672 and CH0516 were
units/mg, and those of ATCarse were 2.20 units/mg and 1.84 units/mg, respectively. It appears that some increase in product yield with C. gluramicum CH0516 is likely due to the increased Akase activity and decreased ATCase activity.
Selection of High Yielding Mutant Strains for the Antifungal Antibiotics KRF-001
KSBB Journal, volume 9, issue 4, 1994, Pages 378~384
An improved method for the selective isolation of high-yielding mutant strains for the production of antifungal antibiotic KRF-001 was investigated. The mutant strain U. V 4, which produces high titer of KRF-001, was selected on the high potency agar plate after ultraviolet light irradiation. The U. V 4 strain produced 2-fold more KRF-001 than the mother strain in production media. Large scale fermentation was performed using the U. V 4 strain in 100
fermenter. The antifungal antibiotic KRF-001 secreted into culture broth was detected by HPLC in 24hrs of fermentation.
Large Scale Purification of KRF-001 on the Preparative HPLC
KSBB Journal, volume 9, issue 4, 1994, Pages 385~394
Large scale purification to get antifungal antibiotic KRF-001 of 90% purity, was investigated using preparative HPLC. Crude KRF-001 was purified by XAD-7 adsorption chromatography, acid precipitation and microfiltration. Microfiltration was the most effective isolation method of crude KRF-001. The purification methods using C18 chromatography was convenient compared with the conventional methods. Delta PAK C18 column and Bonda PAK C18 column were adapted large scale purification of KRF-001. Gradient system of prep HPLC using Delta PAK C18 column was more effective. With these conditions, final recovery of KRF-001 yielded 77%.
Ethanol Fermentation by Pichia Stipitis in a Mixture of Pentoses and Hexoses
KSBB Journal, volume 9, issue 4, 1994, Pages 395~399
P. stipitis CBS5776 was cultivated to examine the characteristics of ethanol fermentation for hexoses (mannose, g1ucose, and galactose) and pentoses(xylose and arabinose). Glucose was the best carbon source among the sugars used in terms of ethanol yield. Glucose was used to produce ethanol with an yield coefficient 0.376g ethanol/g glucose, whereas mannose was converted to produce ethanol with an yield coefficient 0.326g ethanol/g mannose. P. stipitis CBS5776 was also grown in a mixture of sugars to study the pattern of carbon utilization. The yeast utilized glucose and mannose firsts and then galastose and xylose as carbon sources. Arabinose was partially used for biomass when it was present as a sole carbon source, but it was not metabolized at all in a mixture of carbon sources. P. stipitis produced
ethanol with a yield coefficient 0.332 g ethano1/g sugar in a mixture of sugars.
-Galactosidase from Alkalophilic, Thermophilic Baillus sp. TA-11
KSBB Journal, volume 9, issue 4, 1994, Pages 400~405
The conditions for
-galactosidase production from alkalophilic, thermophilic Bacillus sp. TA-11 were investigated. The maximal enzyme production was obtained when the strain was cultured at
for 5 days with fed-batch culture in the optimal medium containing 1.5% lactose, 0.6% yeast extract 0.15%
and initial pH 9.5, and then final enzyme activity under the above conditions was 5200 unit/ml of cell free extract.
Citric Acid Production by Extractive Fermentation
KSBB Journal, volume 9, issue 4, 1994, Pages 406~411
An extractive fermentation process was developed to produce citric acid from g1ucose. Citric acid is a strong inhibitor to this fermentation. A mixture of tertiary amine and oleyl alcohol was used to selectively extract citric acid from the fermentation broth, hence enhancing the productivity by over 200%. Although the toxicity of the solvent was significant in the range of higher than 30% of amine, immobilization in polyurethane foam was useful to protect the cells from the toxicity of the solvent.
Biodegradable Characteristics of Starch-filled Polyethylene Film by Fungi
KSBB Journal, volume 9, issue 4, 1994, Pages 412~417
The biodegradable characteristics of starch-filled polyethylene film by fungi were investigated. Aspergillus niger showed the highest cell growth among five species of fungi used in the experiment. Higher starch content gave the higher growth rate of A. niger; however, the kind of film or cutting direction did not give any effects on the cell growth. The use of vinyl trimethoxysilane for the stronger binding of starch and polyethylene did not inhibit the cell growth. When the starch content was higher than 10%, the elongation of the film decreased significantly after the growth of microorganism in the case of transverse direction samples.
A Study on the Production of
-amylase from Bacillus licheniformis Expressed in E. coli
KSBB Journal, volume 9, issue 4, 1994, Pages 418~427
For the production of
-amylase cloned from Bacillus licheniformis expressed in E. coli, cultivating factors including the concentrations of glucose, maltose and acetic acid were investigated. The results were as follows: 1) Maximum
-amylase yield and maximum specific production rate obtained from glucose source were better than those achieved from maltose source. 2) The optimum production yield of
-amylase was obtained at 1.0ml/
or less of initial acetic acid concentration.
Effects of Concentrations of Glucose and Maltose on the Growth of Bacillus amyloliquefaciens
KSBB Journal, volume 9, issue 4, 1994, Pages 428~435
Cell growth and production of
-amylase, acetic acid and lactic acid were investigated in Bacillus amyloliquefaciens(ATCC 23350) flask culture with various carbon sources. Maximum dry cell density increased with increase in initial maltose concentration. Maximum dry cell density was the highest(1.4g/
) at 10g/
of initial glucose concentration. With 10g/
of initial glucose concentration, maximum specific cell growth rate was obtained. Among the various carbon sources maximum
-amylase production was obtained with 149 unit/ml at 20g/
of initial maltose concentration. With 5g/
of initial maltose concentration, maximum
-amylase production rate was obtained. By increasing carbon source concentration, acetic acid formation decreased. Acetic acid formation was higher in glucose than in maltose. By increasing carbon source concentration, lactic acid formation increased. Lactic acid formation was higher in maltose than in glucose.
Studies on the Optimal Culture Conditions for Itaconic Acid Production by Aspergilus Terreus
KSBB Journal, volume 9, issue 4, 1994, Pages 436~442
The production of itaconic acid by Aspergillus terreus NRRL 1960 was studied. The optimal culture conditional such as pH, inoculum size and medium composition were established. Maximum production of itaconic acid,
, was obtained when the cultivation was carried out at
and pH 2.5 for 7days, with medium containing 5%(w/v) glucose, 0.5%(w/v) NH4Cl, 0.2%(w/v) yeast extract 0.1%(w/v) CaC12, 0.1%(w/v) MgSO4 and 0.2%(w/v) NaCl. A proper medium for inoculum culture was found to be 2%(w/v) malt extract. The batch production of itaconic acid with free cells in a stirredtank reactor was not efficient compared to the shake-flask culture.