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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Fisheries and Aquatic Sciences
Journal Basic Information
Journal DOI :
The Korean Society of Fisheries and Aquatic Science
Editor in Chief :
Volume & Issues
Volume 42, Issue 6 - Dec 2009
Volume 42, Issue 5 - Oct 2009
Volume 42, Issue 4 - Aug 2009
Volume 42, Issue 3 - Jun 2009
Volume 42, Issue 2 - Apr 2009
Volume 42, Issue 1 - Feb 2009
Selecting the target year
Toxicity of Puffer Fish, Takifugu poecilonotus (Heuinjeombok) and Takifugu vermicularis (Gukmaeribok) from Coastal Water of Korea
Kim, Ji-Hoe ; Mok, Jong-Soo ; Son, Kwang-Tae ; Hwang, Hye-Jin ; Oh, Eun-Gyoung ; Yu, Hong-Sik ; Kim, Poong-Ho ;
Korean Journal of Fisheries and Aquatic Sciences, volume 42, issue 1, 2009, Pages 1~7
DOI : 10.5657/kfas.2009.42.1.001
The toxicity of two species of puffer fish, Takifugu poecilonotus (Heuinjeombok) and T. vermicularis (Gukmaeribok) collected from the coastal regions of Korea was determined using a mouse bioassay. In the T. poecilonotus collected in Jeju and Tongyeong, the proportion of toxic specimens containing
mouse units (MU) per gram exceeded 95% for the skin, liver, ovary, and fin, and approximately 30% for the testis and muscles. In each of the organs, the highest toxin levels were 79 MU/g in the muscle, hundreds (158-365) of MU per gram in the fin, intestine, testis, and gallbladder, but thousands (1,147-2,406) of MU per gram in the skin, liver, and ovary. In T. vermicularis collected from Incheon and Gunsan, the proportions of toxic specimens were 100% for the gallbladder, and 56-68% for the skin, fin, liver, and intestine however, no toxic muscle specimens were noted. The highest toxin scores were below 10 mouse units (MU) per gram in the muscle, 20-94 MU/g in the skin and fin, 319 MU/g in the intestine, and thousands (1,548-4,624) of MU per gram in the liver, gonad, and gallbladder. The toxicity in the muscle of T. vermicularis was deemed acceptable for human consumption, whereas the toxicities in the muscle of T. poecilonotus and the skin of both species of puffer fish were significantly high, such that special attention may be required when the fish is intended for human consumption.
Stability of Domoic Acid at Different Temperature, pH and Light
Mok, Jong-Soo ; Lee, Tae-Seek ; Oh, Eun-Gyoung ; Son, Kwang-Tae ; Hwang, Hye-Jin ; Kim, Ji-Hoe ;
Korean Journal of Fisheries and Aquatic Sciences, volume 42, issue 1, 2009, Pages 8~14
DOI : 10.5657/kfas.2009.42.1.008
To prevent the food poisoning originated by consumption of shellfish contaminated with domoic acid, the quantitative analysis of domoic acid is to be very important. The stability of domoic acid at different temperature, pH and light was investigated using high performance liquid chromatography (HPLC). The mean recoveries of domoic acid in the methanol extracts from oyster (Crassostrea gigas), blue mussel (Mytilus edulis), short neck clam (Ruditapes philippinarum) and ark shell (Scapharca broughtonii) were 85.4-104.5%, 94.8-101.2%, 91.0-104.6%, and 95.7-109.6%, respectively. The working solutions of domoic acid standard were very stable for one month at
, and room temperature. And domoic acid in the methanol extract from oyster was stable for a day at
and room temperature, and for a week at
. Therefore, this implies that quantitative analysis for domoic acid must consider the storage conditions of the standard solutions and the methanol extracts from shellfish. The standard solutions adjusted to pH 3-9 were also stable after heating at
for 30 min. The effect of light exposure on domoic acid was tested by exposing the methanol extracts to light. Domoic acid degraded slowly when the samples were kept in the dark (brown vial). However, following the light exposure the photodegradation became more rapid; no detectable domoic acid remained in
of methanol extract after 5 hours.
Determination of Residual Erythromycin Antibiotic in Fishery Products by Liquid Chromatography-electrospray Ionization Mass Spectrometry
Jo, Mi-Ra ; Mok, Jong-Soo ; Lee, Doo-Seog ; Kim, Min-Jung ; Kim, Poong-Ho ;
Korean Journal of Fisheries and Aquatic Sciences, volume 42, issue 1, 2009, Pages 15~19
DOI : 10.5657/kfas.2009.42.1.015
A simple and sensitive method for erythromycin quantification by liquid chromatography electrospray mass spectrometry (LC-MS/MS) in fishery products was developed. Samples were extracted by liquid-liquid extraction using 70% acetonitrile. Lipids were removed by acetonitrile saturated hexane. LC separation was performed on a Shiseido UG C-18 column (
internal diameter.) with a gradient system of 0.2% acetic acid-acetonitrile containing 0.2% acetic acid as a mobile phase at flow rate of 0.2 mL/min. The mass spectrometer was operated in selected reaction monitoring with positive electro-spray interface. Transitions were monitored a m/z
, with m/z
chosen for quantification. Recovery of erythromycin from fish and shrimp fortified at the 10 ng/mL, 50 ng/mL and 100 ng/mL were 91.6-109.4%, 84.4-111.2% and 98.8-109.6% with high precision, respectively. Limits of quantification and limits of detection of erythromycin in both fish and shrimp were 10.0 ng/mL and 1.0 ng/mL, respectively. This analysis method for erythromycin has been proposed for registration in the Korean Official Methods of Food Analysis and has been utilized for fishery products analysis by the Korea Food and Drug Adminstration and the National Fisheries Products Quality Inspection Service.
Characteristics of Ampicillin-Resistant Vibrio spp. Isolated from a West Coastal Area of Korean Peninsula
Lee, Han-Woong ; Lim, Suk-Kyung ; Kim, Mal-Nam ;
Korean Journal of Fisheries and Aquatic Sciences, volume 42, issue 1, 2009, Pages 20~25
DOI : 10.5657/kfas.2009.42.1.020
Thirty-eight Vibrio spp. were isolated from the sea waters harvested from the 22 stations located on the west coast of the Korean peninsula in September 2006. The isolates consisted of V. parahaemolyticus (n=21), V. alginolyticus (n= 16) and V. cholerae non-01 (n=1), among which 35 isolates displayed resistance against two of the tested antibiotics. Among the 38 isolates, 18 isolates exhibited multi-drug resistance against more than four 4 antibiotics. In particular, minimum inhibitory concentration
of ampicillin-resistant isolates were as high as
-lactamase production was examined to analyze the ampicillin-resistance. Some Vibrio spp. isolates produced
-lactamase, however antibiotics resistance pattern and
-lactamase production were not clearly related to each other. A genetic relationship between resistance and gene expression was confirmed in the ampicillin-resistant isolates.
Evaluation of the Optimum Dietary Selenium (Se) Level to Improve Immune Responses in Juvenile Olive Flounder, Paralichthys olivaceus
Lee, Jun-Ho ; Kim, Young-Chul ; Park, Soo-Il ; Bai, Sung-Chul C. ;
Korean Journal of Fisheries and Aquatic Sciences, volume 42, issue 1, 2009, Pages 26~33
DOI : 10.5657/kfas.2009.42.1.026
This experiment was conducted to examine the utilization of added dietary selenium (Se) as an immune stimulant in juvenile olive flounder, Paralichthys olivaceus. Fish averaging
) were fed one of seven semi-purified diets containing 0.56, 1.07, 2.86, 4.56, 43.15, 90.71, or 161.74 mg of Se/kg (
, respectively) for 12 weeks, respectively. At the end of the feeding trial, the fish fed diets containing more than 43.2 mg of Se/kg showed above 90% mortality. There were no significant differences in weight gain, feed efficiency, specific growth rate, protein efficiency ratio, or hematological characteristics among the fish fed the
diets. Se concentrations of the gill, kidney, muscle and liver tissues occurred in dose-dependent manners. Alternative complement pathway activation and the chemiluminescene responses of the fish fed the
diet were significantly higher than those of the fish fed the other diets (P<0.05). These results indicate that the optimum dietary supplementation level of Selenium as selenoyeast could be 1.07 mg of Se/kg based on the non-specific immune responses of juvenile oilve flounder, Paralichthys olivaceus.
Comparative Efficacy of Antifungal Agents for Aquaculture Fish and their Eggs
Lee, Bo-Young ; Lee, Deok-Chan ;
Korean Journal of Fisheries and Aquatic Sciences, volume 42, issue 1, 2009, Pages 34~40
DOI : 10.5657/kfas.2009.42.1.034
In fresh water fish hatcheries and farms, Saprolegniales often cause serious mortality to the fish and their eggs. Malachite green is an effective antifungal agent, but is carcinogenic to fish and humans. Alternative antifungal agents are needed. Presently, we tested various concentrations of MBT-01108 (Opuntia ficus-indica extracts) alone and in combination with bronopol, formalin and sodium chloride (MBT-01108 mixture) on in vitro mycelial growth and in vivo remediation of adult eel, Anguilla japonica, infected with Saprolegnia sp. and fertilized eggs of chum salmon, Oncorhynchus keta, to evaluate the compounds' antifungal efficacy on eyed-egg and hatching rates. MBT-Oll08 mixtures incorporating bronopol and formalin at respective concentrations of 50 and 30 parts per million (ppm), and 100 and 20 ppm were most effective in controlling Saprolegnia in vitro and in vivo (P<0.05). Repeated daily exposures to 50 ppm and 100 ppm MBT-01108 were more effective than exposure every 2-3 days post-fertilization for the inhibition of Saprolegnia infection of rainbow trout, Oncorhynchus mykiss eggs as compared with control (0 ppm).
Histological and Biochemical Analyses on Reproductive Cycle of Gomphina melanaegis (Bivalvia; Veneridae)
Kim, Su-Kyoung ; Lee, Chu ; Kim, Yang-Dae ; Jo, Q-Tae ; Lee, Jong-Ha ; Park, Min-Woo ; Kim, Jae-Won ; Gong, Yang-Geun ;
Korean Journal of Fisheries and Aquatic Sciences, volume 42, issue 1, 2009, Pages 41~47
DOI : 10.5657/kfas.2009.42.1.041
The seasonal reproductive cycle of Gomphina melanaegis collected in the coastal area of Jumunjin, between April 2006 and March 2007, was analyzed. Maturation cycle parameters such as the gonad index (GI), ovarian egg diameter, frequency of developmental stages, protein content, and RNA/DNA variation in the gonads were analyzed monthly for the 40 samples. According to the indices from histological sections, the frequency of gonad developmental stages, and the oocyte diameter, this clam has a long-term partial spawning pattern from March to October. However, GI and nucleic acid values showed that the mature stage is from March to July and that the main spawning season is August. The peak RNA and DNA contents were good indicators of sexual maturation in females and males, respectively. The variation in protein content corresponded with the RNA/DNA ratios.
Gonad Maturation and Spawning of the Blue Crab, Portunus trituberculatus (Miers, 1876) from the West Sea of Korea
Seo, Hyung-Chul ; Jang, In-Kwon ; Cho, Yeong-Rok ; Kim, Jong-Sheek ; Kim, Bong-Rae ;
Korean Journal of Fisheries and Aquatic Sciences, volume 42, issue 1, 2009, Pages 48~55
DOI : 10.5657/kfas.2009.42.1.048
The gonad maturation and spawning of blue crab, Portunus trituberculatus (Miers, 1876) using samples caught by trammer net in the western coast of Korea from January to December. The gonadosomatic index (GSI) showed the highest value of 15.0 in June and lowest value of 1.29 in August. Hepatosomatic index (HSI) showed the lowest in the spawning season from June to July. Thereafter, from August the value started to increase to 5.77 in December, showing an adverse trend against GSI. In the histological studies of the female reproductive organ, the gonad filled with primary oocytes from January to February and secondary oocytes in May. The female gonads mature from June, and mature and immature groups could be distinguished from December to February or March. In August, female developed another cycle of gonad development, showing new primary oocytes in the gonads again. Some of female crabs had receptaculum filled with already active sperm cells in it from March to May. In the time of August to September, almost all the females had the sperm sacs in the receptaculum. From the result, the coupling period estimated from August to September. Total RNA and DNA values of the crabs were highest in May (2.51,
). The DNA value remained unchanged except for the value in May (
). while RNA value was lowest in January (
). The RAN/DNA ratio was the lowest (6.23%) in May and highest (18.05%) in July. The value of correlations coefficient between body weight and fecundity of the crabs was higher that of between carapace width and fecundity.
Feeding Regime of Phyto- and Zoo Live Food Organisms for the Optimum Growth and Survival of Maroon Clown fish, Premnas biaculeatus Early Larvae
Kim, Jong-Su ; Rho, Sum ; Choi, Young-Ung ; Kim, Won-Pyoung ; Jung, Min-Min ;
Korean Journal of Fisheries and Aquatic Sciences, volume 42, issue 1, 2009, Pages 56~62
DOI : 10.5657/kfas.2009.42.1.056
Feeding regimens was investigated for the growth and survival rate of juvenile Maroon clownfish (Premnas biaculeatus) in order to establish a seed production technique for this fish. Three types of feeding regimens were used, and growth and survival were highest under the following regimen: rotifer from day 0, Artemia from day 4, copepod from day 9 and artificial food from day 15, respectively (P<0.05). The average body lengths of the larvae were
in the Nannochloropsis oculata, Isochrysis galbana and control treatments conducted with 3 larvae/L, respectively (P<0.05). There were significant differences in larval growth between the treatments and controls conducted with 6 larvae/L and 9 larvae/L, respectively (P<0.05) but there was no significant difference between the N. oculata and I. galbana treatments (P>0.05). There were no significant differences in larval growth between the treatments at densities of 3-9 larvae/L (P>0.05). At highest density level, the larvae reared in I. galbana had the highest survival rate whereas survival was lowest in the control.
Effects of Adhesive Substrates and Bottom Materials on Release of Fertilized Eggs by Pacific Cod, Gadus macrocephalus
Lee, So-Gwang ; Park, Kyung-Dae ; Gwak, Woo-Seok ;
Korean Journal of Fisheries and Aquatic Sciences, volume 42, issue 1, 2009, Pages 63~67
DOI : 10.5657/kfas.2009.42.1.063
The effects of adhesive substrates and bottom materials on the release of fertilized eggs by the Pacific cod, (Gadus macrocephalus) were examined to find an effective release method. Palm twin as an adhesive substrate displayed a significantly elevated adhesive rate compared to gulf weed. However, the survival rate of the fertilized eggs attached to palm twin was as low as 17.5% 8 days after fertilization (DAF). In contrast, fertilized eggs in the absence of adhesive substrate displayed the highest survival rate (47.0%). Concerning bottom materials, the survival rate of fertilized eggs on sand was significantly high (approximately 51.1%) on 8 and 9 DAF. The observations indicates that adhesive substrates are not needed and spawning ground bottom materials such as sand or mud should be considered when releasing fertilized eggs of Pacific cod.
Horizontal Distribution of Dinoflagellate Resting Cysts in Sediments from the Southeastern Yellow Sea
Hwang, Choul-Hee ; Heo, Seung ; Kim, Chang-Hoon ;
Korean Journal of Fisheries and Aquatic Sciences, volume 42, issue 1, 2009, Pages 68~72
DOI : 10.5657/kfas.2009.42.1.068
To gain a greater understanding of the potential for future harmful algal bloom (HAB) outbreaks and to trace the dispersal paths of dinoflagellates, sediment samples were collected from 13 stations in the southeastern Yellow Sea. 23 different types of dinoflagellate resting cysts were identified from the samples. Protoceratium reticulatum (1-391 cells/g dry weight), Gonyaulax scrippsae (0-254 cells/g dry weight), G. spinifera (0-301 cells/g dry weight) and Alexandrium spp. (ellipsoidal type) (0-76 cells/g dry weight) were the dominant species at all surveyed stations. The overall distribution pattern demonstrated that the resting cyst densities were highest in the offshore area and decreased gradually toward the Korean coast. On the other hand, the composition rate of resting cysts of the heterotrophic dinoflagellate species to the total dinoflagellates was higher in the Korean coast region than in the offshore area. We supposed that this distribution pattern of dinoflagellate resting cysts appeared to be influenced by the hydrographic features and environmental conditions of the Yellow Sea.
Feeding Habits of Released Black Rockfish, Sebastes inermis, in Coastal Waters off Jam Island, Jinhae Bay, Korea
Kim, Gwang-Soo ; Son, Min-Ho ; Kwak, Seok-Nam ; Park, Joo-Myun ; Huh, Sung-Hoi ;
Korean Journal of Fisheries and Aquatic Sciences, volume 42, issue 1, 2009, Pages 73~77
DOI : 10.5657/kfas.2009.42.1.073
Feeding habits of released Sebastes inermis obtained in daily and monthly collections from August 2007 to December 2007 in coastal waters off Jam Island in Jinhae Bay, Korea were assessed. A total 151 individuals of S. inermis (6.3-9.7 cm TL) were recaptured during the study period. Initial food intake of released S. inermis occurred within three days of released. Stomach content index increased with time, however, the percentage of stomachs devoid of contents decreased. Sebastes inermis mainly consumed gammarid and caprellid amphipods, gastropods and mysids. Diet also included small quantities of bivalves, shrimps, copepods and ostracods. Amphipods were mainly consumed at all size groups and consumption of gastropods, bivalves and shrimps increased with increasing fish size.
Size Selectivity of Gill Net for Male Japanese Sandfish (Arctoscopus japonicus) off Gangwon in winter
Jeong, Eui-Cheol ; Park, Hae-Hoon ; Bae, Bong-Seong ; Chang, Dae-Soo ; Kim, Chan-Seob ; Choi, Soo-Ha ; Cha, Hyung-Kee ;
Korean Journal of Fisheries and Aquatic Sciences, volume 42, issue 1, 2009, Pages 78~82
DOI : 10.5657/kfas.2009.42.1.078
We describe a catch experiment and the size selectivity of gill nets for male Japanese sandfish (Arctoscopus japonicus) caught near Gosung, Korea. The experiment, which was conducted between October and December 2007, examined six different mesh sizes: 36.4, 39.4, 45.5, 51.5, 53.0, and 57.6 mm. The catch was mostly Japanese sandfish (91.3%) and some others (8.7%). More male Japanese sandfish were caught with smaller mesh sizes and more females with larger mesh sizes. Relationships between the weight and fork length of the male and female Japanese sandfish were obtained. The relationship between the catch (Y) and mesh size of the gill net (X) was
). The selection curve for male Japanese sandfish caught in the experiment was fit, via Kitahara's method, to a polynomial equation, which was
, where R=l/m, and l and m are the fork length and mesh size, respectively.
Catch fluctuation of the Pound Set Net According to Tide Age in the Coastal Waters of Jeju
Kim, Byung-Yeob ; Seo, Du-Ok ; Lee, Chang-Heon ;
Korean Journal of Fisheries and Aquatic Sciences, volume 42, issue 1, 2009, Pages 83~88
DOI : 10.5657/kfas.2009.42.1.083
The purpose of this paper is to obtain the fundamental data on the catch fluctuation in the pound set net according to the tide age from the catch recorded from the year 1997 to 2004 in the coastal waters of Guideok, Jeju. Total catch by the pound set net had a little connection with the tide age. During increasing tide, total catch were reduced slightly from the neap tide to the high tide while there seemed to be a little sign of rise in the total catch until decreasing tide. But in the relation between the catches and the tide age, the level of the correlation coefficient chosen at
was not significant. Therefore, the catch of the pound set net seemed not to be influenced by the tide age. In addition, CPUE at the high tide was higher than that at the neap tide. When the catch per operating frequency was graded in the order under 50 kg, 50-100 kg and 100-200 kg, the frequency rate by the pound set net was 38%, 19% and 19%, respectively.