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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Fisheries and Aquatic Sciences
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Journal DOI :
The Korean Society of Fisheries and Aquatic Science
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Volume & Issues
Volume 47, Issue 6 - Dec 2014
Volume 47, Issue 5 - Oct 2014
Volume 47, Issue 4 - Aug 2014
Volume 47, Issue 3 - Jun 2014
Volume 47, Issue 2 - Apr 2014
Volume 47, Issue 1 - Feb 2014
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Nutritional and Physiologically Active Characterizations of the Sea Squirt Halocynthia roretzi Sikhae and the Seasoned Sea Squirt
Kim, Poong Ho ; Kim, Min Ji ; Kim, Ji Hye ; Lee, Ji Sun ; Kim, Ki Hyun ; Kim, Hyeon Jeong ; Jeon, You-Jin ; Heu, Min Soo ; Kim, Jin-Soo ;
Korean Journal of Fisheries and Aquatic Sciences, volume 47, issue 1, 2014, Pages 1~11
DOI : 10.5657/KFAS.2014.0001
This study was carried out to investigate nutritional and physiologically active characterizations of sikhae and the seasoned products from the sea squirt Halocynthia roretzi. The total taste values of sikhae fermented for 4 and 5 days were 10.9 and 15.4, respectively, which was lower than for commercially seasoned sea squirts. The sikhaes contained mostly glutamic and aspartic acids. The total amino acid contents of sikhaes fermented for 4 and 5 days were 5.5 and 6.0 g/100 g, respectively, which were lower than those of commercial seasoned-sea squirts or similar. An amount of 100 g of sikhae and its seasoned products contained P, K, Mg and Fe, and these minerals, which are deemed good for our health, were at 10% above the recommended daily requirements. The functional properties of sikhae fermented for 4 and 5 days were as follows: for ACE inhibiting activity, 69 and 69.5%, respectively; for antioxidative activity, 28.9 and 29.3%, respectively; for xanthine oxidase inhibitory activity, 52.8 and 53.1%, respectively; and for
-glucosidase inhibitory activity, 2.4 and 1.4%, respectively. Antimicrobial activity of the 5 day fermented sikhae against Vibrio parahaemolyticus and Staphyloccus aureus was detected in 8 mm and in 7 mm against Escherichia coli.
Effects of Vacuum Cooling Followed by Water Spraying on the Quality of Precooked Skipjack Katsuwonus pelamis
Lee, Tae-Hun ; Koo, Jae-Geun ;
Korean Journal of Fisheries and Aquatic Sciences, volume 47, issue 1, 2014, Pages 12~17
DOI : 10.5657/KFAS.2014.0012
The cooling of precooked skipjack Katsuwonus pelamis is a critical thermal process in tuna canning because it affects the quality and yield of the canned tuna, as well as productivity. The combined method of vacuum cooling followed by water spraying (VC-WS) was investigated to increase cooling rates, and prevent loss of yield of the precooked tuna during vacuum cooling. For VC-WS, the precooked skipjack was cooled to
by vacuum at 31 mmHg and then wetted by spraying water for 2 min. The effects of VC-WS on cooling times, cooling loss, color, texture and lipid oxidation of the precooked tuna were compared with conventional spray cooling (SC).The cooling times for precooked skipjack from
were 11 min for VC-WS and 145 min for SC. The cooling losses were 1.7 % for VC-WS and 1.6 % for SC. Peroxide and thiobarbituric acid (TBA) values of VC-WS were lower than those of SC. The loin of the VC-WS-treated skipjack was brighter and harder than the SC loin, as indicated by higher lightness and hardness values. Based on these results, we believe that the VC-WS process could compensate for the cooling loss of vacuum cooling and minimize changes in quality that occur during cooling of precooked skipjack tuna.
Antibacterial Activity of Rhus javanica against the Fish Pathogens Vibrio ichthyoenteri and Streptococcus iniae
Kim, Kyoung-Hoon ; Kim, Ah Ra ; Cho, Eun-Ji ; Joo, Seong-Je ; Park, Jong-Hoon ; Moon, Ji-Young ; Yum, Jong-Hwa ; Kim, Tae Hoon ; Kwon, Hyun-Ju ; Lee, Hyun-Tai ; Kim, Young-Man ; Lee, Eun-Woo ;
Korean Journal of Fisheries and Aquatic Sciences, volume 47, issue 1, 2014, Pages 18~22
DOI : 10.5657/KFAS.2014.0018
The antibacterial activities of methanol extracts of 19 commercial herbal medicines were measured against the fish pathogens Vibrio ichthyoenteri and Streptococcus iniae, which cause several fish diseases. Rhus javanica showed the strongest antibacterial activity against V. ichthyoenteri and S. iniae. The methanol extract of R. javanica was extracted further using several organic solvents with different polarities. The extract from the ethyl acetate fraction showed strong activity against both fish pathogens. The minimum inhibitory concentration (MIC) of the R. javanica extract was
for V. ichthyoenteri and
for S. iniae. Further purification and isolation of the active compound (s) responsible for these activities and further study of the synergistic effect using combinations of antibiotics against pathogenic bacteria are needed.
The Effects of a Dietary Edwardsiella tarda Specific Bacteriophage and Bacillus subtilis Mixture on Innate Immune Responses and Antibacterial Activity of Nile tilapia Oreochromis niloticus
Baek, Min Suk ; Hwang, Yo Sep ; Choi, Sanghoon ;
Korean Journal of Fisheries and Aquatic Sciences, volume 47, issue 1, 2014, Pages 23~30
DOI : 10.5657/KFAS.2014.0023
The present study investigated the effects of dietary Edwardsiella tarda (E. tarda) specific bacteriophage (phage) and Bacillus subtilis (B. subtilis) mixture on innate immune responses and antibacterial activity of Nile tilapia, Oreochromis niloticus. In a dietary experiment, tilapia were fed the control diet (C), a phage-only supplemented diet (P), a B. subtilis only supplemented diet (B), or a B. subtilis and phage mixed diet (B+P). A respiratory burst and significant increase in lysozyme activity (P<0.05) were noted in the B+P group, as compared to other groups after 4 days of feeding. The B group showed a significant (P<0.05) increase in respiratory burst and lysozyme activity versus the C and P groups, whereas no significant increases (P<0.05) were observed in the P and C groups.
was significantly up-regulated in the B+P group versus other groups after 8 days of feeding (P<0.05). In vivo antibacterial activity was significantly enhanced in the B+P fed group, as compared to other groups (P<0.05) after 7 days of E. tarda challenge. A significant (P<0.05) increase in antibacterial activity was seen in the B group, as compared to C or P groups after 14 days of feeding. These results suggest that a B. subtilis and phage mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.
Isolation and Identification of DHA-Rich Marine Microorganism
Jeong, U-Cheol ; Choi, Byeong-Dae ; Choi, Jong-Duck ; Kang, Seok-Joong ;
Korean Journal of Fisheries and Aquatic Sciences, volume 47, issue 1, 2014, Pages 31~38
DOI : 10.5657/KFAS.2014.0031
Docosahexaenoic acid (DHA, 22:6n-3) and ecosapentaenoic acid (20:5n-3) have attracted increasing attention since the first epidemiological report on the importance of n-3 essential fatty acids. It is thought that DHA has important functions in brain and retinal tissues. Thraustochytrids, a group of marine protists, are capable of heterotrophic growth, and are potential omega-3 producers for industrial use, especially the members of the Schizochytrium and Thraustochytrium genera. The aims of this work were to isolate, identify and screen thraustochytrids from 17 different locations. Twenty-three isolates were screened for biomass, total fatty acid (TFA) and DHA content. Analysis of the fatty acid methyl esters revealed four distinct clusters biomass ranged from
, and lipid and DHA contents ranged from
biomass, respectively. B-12 isolates were screened for biomass (
), TFA (
) and DHA (47.01%, w/w) content. C-6 isolates were also screened for biomass (
), TFA (
) and DHA (49.41%, w/w) content. The 18S rRNA gene sequencing results identified Schizochytrium mangrovei as B-12 and Crypthecodium cohnii as C-6.
Cryopreservation of Common Korean Bitterling Acheilognathus signifer Sperm
Jeong, Min Hwan ; Min, Byung Hwa ; Park, Mi Seon ; Myeong, Jeong-In ; Im, Je Hyun ; Lim, Han Kyu ; Hwang, Hyung-Kyu ;
Korean Journal of Fisheries and Aquatic Sciences, volume 47, issue 1, 2014, Pages 39~44
DOI : 10.5657/KFAS.2014.0039
This study aimed to find an optimal diluent and cryoprotective agent (CPA) during cryopreservation of common Korean bitterling Acheilognathus signifier sperm. The bitterling is an endangered species in Korea, and this study will enable conservation and further technical development of artificial seed production. We tested the effects of cryopreservation and toxicity by the type of diluent and CPA. The optimal combination of diluent and CPA for cryopreservation was 300 mM glucose+10% methanol, resulting in a survival rate and sperm activity index (SAI) of
respectively, and no significant difference compared to fresh sperm. The survival rate and SAI of post-thawed sperm was
respectively, which was significantly higher than had been achieved with other diluents and CPAs.
In vitro Anti-bacterial and Anti-scuticociliate Activities of Extract and Bromophenols of the Marine Red Alga Polysiphonia morrowii with Structure-activity Relationships
Kang, So Young ; Lee, Sang-Yun ; Choi, Jun-Ho ; Jung, Sung-Ju ;
Korean Journal of Fisheries and Aquatic Sciences, volume 47, issue 1, 2014, Pages 45~51
DOI : 10.5657/KFAS.2014.0045
Scuticociliates are regarded as serious pathogens in marine aquaculture worldwide. In Korea, they cause mass-mortalities in fish such as the commercially important olive flounder Paralichthys olivaceus. In particular, mixed infections of scuticociliates with pathogenic bacteria have been commonly reported. During efforts to identify natural marine-algae derived products that possess anti-bacterial and anti-scuticociliate properties, we found that an 80% methanolic extract of the red alga Polysiphonia morrowii Harvey exhibits both anti-scuticociliate activity against Miamiensis avidus, which is a major causative agent of scuticociliatosis, and anti-bacterial activities against fish pathogenic bacteria. Activity-guided fractionation and isolation of the 80% methanolic extract of P. morrowii yielded three bromophenols, which were identified as 3-bromo-4,5-dihydroxybenzyl methyl ether (1), 3-bromo-4,5-dihydroxybenzaldehyde (2) and urceolatol (3) based on spectroscopic analyses. 3-bromo-4,5-dihydroxybenzyl methyl ether (1) showed the highest anti-bacterial and anti-scuticociliate activities, with a minimal inhibitory concentration (MIC) of
(against Vibrio anguillarum) and minimal lethal concentration (MLC) of 62.5 ppm (in seawater). Investigations of the anti-bacterial and anti-scuticociliate activities of seventeen bromophenol derivatives, including the three isolated natural bromophenols, showed that the existence of an electron donating group or atom with a non-covalent electron pair at
of the 2-bromophenol structure may be important in anti-scuticociliate activity. These findings suggest that the extract and bromophenol derivatives of P. morrowii may provide useful alternatives in aquaculture anti-scuticociliate therapies.
Morphological and Genetic Variation of Two Populations of Platichthys stellatus (Pleuronectidae, PISCES) from the East Sea
Jeong, Yong Tae ; Baek, Hea Ja ; Kim, Jin-Koo ;
Korean Journal of Fisheries and Aquatic Sciences, volume 47, issue 1, 2014, Pages 52~58
DOI : 10.5657/KFAS.2014.0052
Morphological and genetic variation of two populations of Platichthys stellatus were investigated based on 30 individuals each, collected from Uljin (seedling release area) and Pohang (control) in Korea. Morphological analyses demonstrated that the two populations of P. stellatus were well distinguishable in body color of the blind side and fin shape. Mitochondrial DNA control region analysis indicated no significant differences between the two populations (
, P>0.05). We also analyzed microsatellite DNA loci of the two populations using six markers. Observed heterozygosity (
) and expected heterozygosity (
) were 0.550 and 0.592, respectively, in P. stellatus from Uljin, but 0.700 and 0.737 in P. stellatus from Pohang. An index of differentiation in genetic structure revealed significant differences between the two populations (
, P<0.05). Our results suggest that the Uljin population may be comprised of released P. stellatus, whereas the Pohang population may be wild P. stellatus, highlighting the necessity of continuous monitoring of the two populations.
Seasonal Characteristics of Todarodes pacificus Paralarval Distribution in the Northern East China Sea
Kim, Jung Jin ; Kim, Cheol-Ho ; Lee, Joon-Soo ; Kim, Suam ;
Korean Journal of Fisheries and Aquatic Sciences, volume 47, issue 1, 2014, Pages 59~61
DOI : 10.5657/KFAS.2014.0059
We conducted eight surveys in the northern East China Sea (ECS) in winter (February - April), summer (July), and autumn (October) 2004-2009, to investigate the seasonal distribution of T. pacificus. A total of 482 paralarvae, ranging in mantle length (ML) from 1.0 - 17.0 mm, were collected at 73 out of 181 stations. There were higher numbers of paralarvae during the winter and summer months than in the autumn. There was significant seasonal variation in the paralarval mantle lengths; mantle lengths were longer in winter (April) than in summer (July). The position of oceanic fronts in the study area played an important role in restricting paralarval distribution along the inshore edge of the Tsushima Warm Current (TWC). When the TWC expanded to western Jeju Island in winter and autumn, the paralarval distribution range extended to include western Jeju Island. However, when the TWC was located southeast of Jeju Island in the summer, paralarvae were distributed along the frontal zone off southeast Jeju Island. Sites at which paralarval mantle length was <2.0 mm ML indicated that the spawning ground were likely to be within the northern ECS in winter and summer, but north of the study area in autumn.
Molecular Phylogenetic Status of Korean Hemiculter Species
Kim, Maeng Jin ; Lee, Jae-Seong ; Song, Choon Bok ;
Korean Journal of Fisheries and Aquatic Sciences, volume 47, issue 1, 2014, Pages 72~78
DOI : 10.5657/KFAS.2014.0072
We investigated the molecular phylogeny and genetic differences among local populations of Korean Hemiculter fishes based on their mitochondrial cytochrome b gene sequences. Our results indicated that Hemiculter leucisculus populations in China were clearly divided into two groups. The first group (Group 1) included the populations of the Yangtze River and its surrounding areas (including the Qiantangjiang, Lingjiang, Jiulongjiang, and Minjiang rivers); the second group (Group 2) contained local populations from southern China (including the Nanliujiang, Zhujiang, Wanquanhe, Qianjiang, and Nandujiang rivers). The Korean Hemiculter eigenmanni differed in its cytochrome b gene sequence by 0.6-1.0% from the Chinese H. leucisculus (Group 1), which inhabited the Yangtze River and its surrounding areas, suggesting they were phylogenetically close and likely to be the same species. The Korean H. leucisculus differed from the Chinese H. leucisculus (both Groups 1 and 2) by 8.1-9.5%, indicating a very distant phylogenetic relationship; however, the Korean H. leucisculus differed from Hemiculter bleekeri by only 0.5-0.7%, showing intraspecific nucleotide differences. We conclude that the taxonomic relationship between the Korean H. leucisculus and H. bleekeri requires further investigation using type specimens.
Molecular Identification and Morphological Comparison of Juveniles of Two Collichthys Species (Pisces: Sciaenidae) from the Yellow Sea
Lee, Soo Jeong ; Kim, Jin-Koo ;
Korean Journal of Fisheries and Aquatic Sciences, volume 47, issue 1, 2014, Pages 79~83
DOI : 10.5657/KFAS.2014.0079
Thirty five juveniles belonging to the genus Collichthys were collected using a bag net at Gang-wha-do, in the eastern Yellow Sea, between July and September, 2012, and identified using combined genetic and morphological methods. We sequenced 316 base pairs of mitochondrial DNA cytochrome c oxidase subunit I of 35 individuals, of which 22 individuals were identified as Collichthys niveatus (12.9-47.6 mm in SL) and 13 as Collichthys lucidus (13.4-40.3 mm SL). Morphologically, the number of occipital crests, an important taxonomic character during the adult stage, could not distinguish the two species during the juvenile stage, but the shape of the first anal fin spine clearly distinguished the two species even among juveniles.