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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Fisheries and Aquatic Sciences
Journal Basic Information
Journal DOI :
The Korean Society of Fisheries and Aquatic Science
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Volume & Issues
Volume 48, Issue 6 - Dec 2015
Volume 48, Issue 5 - Oct 2015
Volume 48, Issue 4 - Aug 2015
Volume 48, Issue 3 - Jun 2015
Volume 48, Issue 2 - Apr 2015
Volume 48, Issue 1 - Feb 2015
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Effects of Lactic Acid Bacterial Fermentation on the Antioxidant and Anti-inflammatory Activity of Brown Algae Eisenia bicyclis Extract
Han, Hae-Na ; Eom, Sung-Hwan ; Kim, Ji-Hoon ; Kim, Deok-Hoon ; Kim, Song-Hee ; Kim, Yunhye ; Yeom, Seung-Mok ; Kim, Young-Mog ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 2, 2015, Pages 151~157
DOI : 10.5657/KFAS.2015.0151
This study was conducted to evaluate the effect of lactic acid bacterial fermentation on the antioxidant and anti-inflammatory activity of an edible brown alga, Eisenia bicyclis. Lactic acid bacteria were inoculated into and cultivated in E. bicyclis water extract. The antioxidant activity of the extract was assayed before and following fermentation. Antioxidant activity was determined by assaying the levels of radical scavenging activity against 2,2'-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical and alkyl radical. The lactic acid bacterial fermentation of E. bicyclis extract resulted in enhanced antioxidant activity. The greatest enhancement of antioxidant activity was seen in the DPPH radical scavenging assay, in which E. bicyclis extract was fermented by Pediococcus pentosaceus MBP-34 strain for 12 h. This fermented extract also exhibited higher inhibitory activity (96.66%) on nitric oxide production compared with other lactic acid bacterial fermented extracts or raw extract (189.60%). In conclusion, fermentation by bacterial strain is an attractive strategy for developing value-added food ingredients.
A Lateral Flow Immunoassay Kit for Detecting Residues of Four Groups of Antibiotics in Farmed Fish
Jo, Mi Ra ; Son, Kwang Tae ; Kwon, Ji Young ; Mok, Jong Soo ; Park, Hong Jae ; Kim, Hyun Yong ; Kim, Gyung Dong ; Kim, Ji Hoe ; Lee, Tae Seek ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 2, 2015, Pages 158~167
DOI : 10.5657/KFAS.2015.0158
A lateral flow immunoassay kit based on antigen-antibody interactions was developed to detect residues of beta-lactams, quinolones, tetracyclines, and sulfonamides in farmed fish. Group-specific antibodies showing cross-reactivity with other antibiotics in the same group were produced in rabbits. The rabbits were immunized eight times to obtain the maximum titers. Antibodies were extracted from the antisera collected from the immunized rabbits and produced group-specific reactions with antibiotics from the four groups. A kit was prepared that optimize conditions for the antigen-antibody reaction, using colloidal gold conjugated antibodies, and was designed to detect the four groups of antibiotics simultaneously. The kit enabled the detection of antibiotics in the four groups at below maximum residue limits (MRLs), which were
for beta-lactams, and
for quinolones. The cross-reactivity of the antibodies ranged from 10-80% for the sulfonamides, 20-100% for tetracyclines, 38-100% for quinolones, and 20-100% for the beta-lactams, confirming that the antibodies were group specific. The test kit was used 30 times to examine spiked antibiotics at the limits of detection (LODs) and all produced positive results, indicating high sensitivity. The LODs for the assay ranged from 4-20 ng/mL for beta-lactams, 25-50 ng/mL for sulfonamides, 20-100 ng/mL for tetracyclines, and 30-80 ng/mL for quinolones, and there were no false negative reactions at above these LODs. In addition, all of the LODs of the developed kit were correlated with high-performance liquid chromatography (HPLC) data. Our lateral flow immunoassay kit can simultaneously detect antibiotic residues from a large number of fish samples rapidly, strengthening the safety of domestic farmed and imported fish.
Characteristics of the Shells and Calcined Powders from the Butter Clam Saxidomus purpuratus and Littleneck Clam Ruditapes philippinarum as a Natural Calcium Resource
Kim, Jin-Soo ; Jung, Nam Young ; Soo Jang, Jeong ; Lee, Hyun Ji ; Park, Sung Hwan ; Kim, Min Joo ; Heu, Min Soo ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 2, 2015, Pages 168~177
DOI : 10.5657/KFAS.2015.0168
Shell waste from the butter clam Saxidomus purpuratus and littleneck clam Ruditapes philippinarum is a large by-product of shellfish aquaculture, and it is desirable to convert it into value-added products for industrial applications. In this study, calcium carbonate (CaC) polymorphs from butter clam (BCSP) and littleneck clam (LCSP) shell powders and commercial CaC were characterized using Fourier transmission infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The results revealed that the optimal calcination conditions to eliminate organic substances and improve solubility for both BCSP and LCSP were
for 8 h in an electrical furnace. Calcination improved the white index of the butter clam (BCCP) and littleneck clam (LCCP) calcined powder compared with shell powders. The calcium content in BCCP (51.1%) was higher than that of LCCP (44.9%) or commercial calcium oxide (CaO, 44.7%). The XRD patterns of BCCP and LCCP were similar to that of CaO. Cubic-like crystals of CaC and irregular crystals of BCCP and LCCP were observed by SEM. The FT-IR and XRD analyses revealed the presence of calcite and aragonite in the BCSP and aragonite in the LCSP, whereas the CaC contained calcite. These results indicate that butter and littleneck clam shells are potential biomass resources for calcium carbonate and calcium oxide.
Recovery and Fractionation of Serine Protease Inhibitors from Bastard Halibut Paralichthys olivaceus Roe
Kim, Hyung Jun ; Lee, Hyun Ji ; Park, Sung Hwan ; Jeon, You-Jin ; Kim, Jin-Soo ; Heu, Min Soo ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 2, 2015, Pages 178~186
DOI : 10.5657/KFAS.2015.0178
Protease inhibitors (PI) of trypsin and papain as target proteases from the roe of bastard halibut Paralichthys olivaceus were fractionated out using ammonium sulfate precipitation (A), DEAE 650M anion exchange chromatography (D), and Sephacryl S-300 gel filtration (S). The recovery percentages of the fractions with the strongest inhibitory activity for each fractionation method were 13% for the A4 fraction, 21.2% for the D3 fraction, and 21.3% for the S2 fraction, with specific inhibitory activities of the fractions toward trypsin and casein of 168, 139, and 218 U/mg, respectively, while no inhibition of papain was observed. The
for the trypsin-specific substrate
-arginine-p-nitroanilide (BAPNA) was 0.65, 1.55, 2.26, and 2.85 mg/mL for the A4, S2, A3, and D3 fractions, respectively. These results suggest that chromatographic fractionation methods (D and S) based on the molecular mass and charge of the protein were more effective at fractionating PI than was ammonium sulfate precipitation based on protein solubility, and that the bastard halibut roe extract acts as a serine protease inhibitor. Therefore, the PI fraction from fish roe might be useful for inhibiting proteases in foodstuffs, and could constitute an alternative food-grade inhibitor for the surimi industry.
Dietary Effects of Polymannuronate Added to Hamburger Buns on Lipid Metabolism in Rats
Joh, Ihn-Seon ; Kim, In-Hye ; Kwon, Mi-Jin ; Nam, Taek-Jeong ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 2, 2015, Pages 187~192
DOI : 10.5657/KFAS.2015.0187
The dietary fiber alginic acid has no nutritional value; however, it decreases the utilization of nutrients by the body and can prevent obesity, hyperlipidemia, arteriosclerosis, constipation, and colon cancer. The low-molecular-weight alginic acid polymannuronate improves serum and liver lipid metabolism by decreasing cholesterol levels in high-cholesterol groups and may be used to control high blood pressure. Previously, we showed that polymannuronate, a physiologically active agent from seaweed, has a lipid-lowering effect and preventative role in colon cancer. In addition, the differentiation of polymannuronate-treated adipocytes was inhibited, triggering decreased leptin expression. This study examined ways to increase dietary satisfaction with and improve the nutritional quality of polymannuronate using hamburger buns supplemented with polymannuronate. Twenty male Sprague-Dawley rats were divided into two groups: the control group was fed hamburger buns containing 6% gluten, while the experimental group was fed hamburger buns containing 6% gluten and 10% polymannuronate. The serum triglyceride, phospholipid, and total and free cholesterol levels of the rats in the experimental group were decreased compared with those of the controls. The serum aspartate aminotransferase activity levels did not differ between the two groups. This study demonstrates that polymannuronate has beneficial effects on lipid metabolism and may be commercially useful.
Early Growth Changes in Juvenile Abalone Haliotis discus discus Produced by Irradiation with Low-dose
Co Gamma Rays
Jwa, Min Seok ; Yeo, In-Kyu ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 2, 2015, Pages 193~199
DOI : 10.5657/KFAS.2015.0193
We observed that the growth and physiological change in Haliotis discus discus by low-dose irradiation with gamma ray.Irradiation with gamma ray was undertaken by using the low-level irradiation facility (
) in cooperation with the Institute of Nuclear Science and Technology at Jeju National University. The parent abalones were attached one by one and then fixed by using the rubber band to the front side of the fabricated case for irradiation with gamma ray. The experimental plots of irradiation with gamma ray were set as 10, 15, 20 and 25 Gy and the 25 female abalones and 10 male abalones were utilized for each experimental plot. The sperms and eggs were fertilized by setting an interval for each dose to prevent mixing with other experimental plots when fertilizing the sperms and eggs for each dose of irradiation with gamma ray. As for the fertility, it was confirmed to be 85% the control and 10 Gy groups, whereas it was found to be 80%, 65% and 50% in the 15 Gy, 20 Gy and 25 Gy groups, respectively. As a conclusion, the hatching rate and attachment rate were higher at 10 and 15 Gy than the other experimental plots, and the growth rate was higher at 20 Gy than the other experimental plots. Also the changes in lysozyme activity in accordance with the stress of water temperature were found to have a significant increase in the other experimental plots as compared with the control plot at the end of 0 h. The changes in lysozyme activity have remained constant in all the experimental plots at the end of 12 h. These results allowed us to confirm that lysozyme was undertaking the biodefense action by reacting sensitively to the stress of water temperature in the control experimental plot. As for the other experimental plots, they are believed to avoid the biodefense mechanism due to the high degree of anti-parasite mechanism and anti-viral mechanism. Thus, it is believed that it would be imperative to conduct development and research on breeds that were potent for environmental tolerance by applying the method of irradiation with gamma ray to other marine animals and plants.
A Quantitative Analysis of Greenhouse Gas Emissions from the Danish Seine Fishery using Life Cycle Assessment
Lee, Jihoon ; Lee, Chun-Woo ; Kim, Jieun ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 2, 2015, Pages 200~206
DOI : 10.5657/KFAS.2015.0200
The fishing industry has a negative effect on the environment due to greenhouse gas (GHG) emissions with the high use of fossil fuels, the destruction of underwater ecosystems by bottom trawls, reduction in resources by fishing, and altered ecosystem diversity. GHG emissions from fisheries were discussed at the Canc
n meeting in Mexico in 1992 and are part of the Kyoto protocol in 2005. However, few studies have investigated the GHG emissions from Korean fisheries. To find a way to reduce GHG emissions from fisheries, quantitative analysis of GHG emissions from the Korean fishery industry is needed. Therefore, this study investigated the GHG emissions from the Korean Danish seine fishery using the life cycle assessment (LCA) method. The system boundary and input parameters for each process level are defined for the LCA analysis. The fuel-use coefficient of the fishery is also calculated. The GHG emissions from the representative fish caught by the Danish seine fishery are considered and the GHG emissions for the edible weight of fishes are calculated, considering consumption in different areas and different slaughtering processes. The results will help to understand the GHG emissions from Korean fisheries.
Analysis and Classification of Broadband Acoustic Echoes from Individual Live Fish using the Pulse Compression Technique
Lee, Dae-Jae ; Kang, Hee-Young ; Kwak, Min Son ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 2, 2015, Pages 207~220
DOI : 10.5657/KFAS.2015.0207
This study identified the species-specific, frequency-dependent characteristics of broadband acoustic scattering that facilitate classifying fish species using the pulse compression (PC) technique. Controlled acoustic scattering laboratory experiments were conducted with nine commercially important fish species using linear chirp signals (95-220 kHz) over an orientation angle range of
in the dorsal plane at approximately
increments. The results suggest that the angular-dependent characteristics of the broadband echoes and the frequency-dependent variability in target strength (TS) were useful for inferring the fish species of interest. The scattering patterns in the compressed pulse output were extremely complex due to morphological differences among fish species, but the x-ray images strongly suggested that spatial separation correlated well with scattering for the head, skeleton, bone, otoliths, and swim bladder within each specimen.
Time-Frequency Analysis of Broadband Acoustic Scattering from Chub Mackerel Scomber japonicus, Goldeye Rockfish Sebastes thompsoni, and Fat Greenling Hexagrammos otakii
Lee, Dae-Jae ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 2, 2015, Pages 221~232
DOI : 10.5657/KFAS.2015.0221
Broadband echoes measured in live chub mackerel Scomber japonicus, goldeye rockfish Sebastes thompsoni, and fat greenling Hexagrammos otakii with different morphologies and internal characteristics were analyzed in time and frequency domains to understand the species-specific echo feature characteristics for classifying fish species. The mean echo image for each time-frequency representation dataset obtained as a function of orientation angle was extracted to mitigate the effect of fish orientation on acoustic scattering. The joint time-frequency content of the broadband echo signals was obtained using the smoothed pseudo-Wigner-Ville distribution (SPWVD). The SPWVDs were analyzed for each echo signature of the three fish species. The results show that the time-frequency analysis provided species-specific echo structure patterns and metrics of the broadband acoustic signals to facilitate fish species classification.
Changes in the Orientation and Frequency Dependence of Target Strength due to Morphological Differences in the Fish Swim Bladder
Lee, Dae-Jae ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 2, 2015, Pages 233~243
DOI : 10.5657/KFAS.2015.0233
Controlled broadband acoustic scattering laboratory experiments were conducted using a linear chirp signal (95-220 kHz), and x-ray images of live and model fish with an artificial swim bladder were analyzed to investigate the changes in orientation and frequency dependence of target strength (TS) due to morphological differences in fish swim bladders. The broadband echoes from live and model fish were measured over an orientation angle range of
in the dorsal plane and in approximately
increments. The location of nulls in the simulated echo response of the SINC [sinc function] model was overlaid on the TS map, showing the orientation and frequency dependence of fish TS, and they matched very well. It was possible to infer the equivalent fish scattering size (or swim bladder) using the null spacing in the experimentally obtained broadband TS map. Good agreement was observed for inferring the equivalent scattering size between the SINC model and the broadband echoes measured for the three fish species (black scraper Thamnaconus modestus; goldeye rockfish Sebastes thompsoni; and whitesaddled reef fish Chromis notatus). Some results of this inference are discussed.
The Effects of Natural Food Additives on the Self-life and Sensory Properties of Shucked and Packed Pacific Oyster Crassostrea gigas
Jeong, Eun-Tak ; Han, Hae-Na ; Kim, Yunhye ; Lee, Eun-Hye ; Kim, Deok-Hoon ; Kim, Ji-Hoon ; Yeom, Seung-Mok ; Kim, Young-Mog ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 2, 2015, Pages 244~248
DOI : 10.5657/KFAS.2015.0244
We explored the efficiency of natural antibacterial agents used to enhance the self-life and sensory properties of shucked and packed Pacific oyster Crassostrea gigas, which are in high demanded. First, we screened natural resources exhibiting antibacterial activity against food spoilage and pathogenic bacteria. Of theses, ignited oyster shell powder (IOS) and the natural food preservative, lactic acid bacteria fermented powder (LBF), were selected for further study considering the efficacy, mass production, and cost. The addition of 0.1% IOS (W/V) and 0.5% LBF (W/V) to shucked and packed oyster optimally extended the shelf-life without affecting the sensory evaluation. The results obtained in this study will provide a clue to enhance self-life in raw oyster products.