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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Fisheries and Aquatic Sciences
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Journal DOI :
The Korean Society of Fisheries and Aquatic Science
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Volume & Issues
Volume 48, Issue 6 - Dec 2015
Volume 48, Issue 5 - Oct 2015
Volume 48, Issue 4 - Aug 2015
Volume 48, Issue 3 - Jun 2015
Volume 48, Issue 2 - Apr 2015
Volume 48, Issue 1 - Feb 2015
Selecting the target year
Sensory and Nutritional Characterizations of Mottled Skate Beringraja pulchra Caught off Ulleung Island, Korea
Lim, Yang-Jae ; Jo, Hyun-Su ; Jeong, Gyeong-Suk ; Hwang, Bo-Kyu ; Kang, Sang In ; Heu, Min Soo ; Kim, Jin-Soo ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 3, 2015, Pages 275~283
DOI : 10.5657/KFAS.2015.0275
This study compared the sensory and nutritional characteristics of mottled skate Beringraja pulchra caught off Ulleung Island (U-MS), Korea, with those caught off Daecheong Island (D-MS). Based on their trichloroacetic acid-soluble nitrogen content, free amino acid content, and taste value, the taste of intermediate weight female U-MS was superior to that of both other U-MS and D-MS. The urea content of U-MS was higher in females than males and increased in the order heavy>intermediate>light individuals. There was, however, no difference in urea content between U-MS and D-MS. The taste and flavor characterization results suggest that the level of ammonia, which was converted from urea, influenced the quality of fermented U-DS. With the exception of the light U-DS, the other U-MS exhibited no difference in terms of hardness. The total amino acid content of U-MS was higher in females than males and increased in the order intermediate > heavy > light individuals. The total amino acid content of U-MS was also higher than that of D-MS. The major amino acids of all MSs were aspartic acid, glutamic acid, leucine, and lysine.
Effects of Preprocessing on Quality of Fermented Red Snow Crab Chionoecetes japonicus Sauce
Lim, Ji-Hoon ; Jeong, Jee-Hee ; Jeong, Min-Jung ; Jeong, In-Hak ; Kim, Byoung-Mok ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 3, 2015, Pages 284~292
DOI : 10.5657/KFAS.2015.0284
We explored preprocessing-mediated quality changes in red snow crab fish sauce. A control (C) group and groups treated with autolysis (A), boiling (B), enzymatic hydrolysis (E), and addition of Aspergillus oryzae (K) were formed. The titratable acidity of the K group increased with storage time, whereas that of groups C, A, B, and E decreased. The total and amino nitrogen contents initially increased on storage of all samples, but decreased in later periods. The total plate count (TPC) of the K group was initially 5.26 log CFU/mL and increased to 7.28 log CFU/mL at 3 months of storage. The TPCs of the C, A, B, and E groups were initially <5.00 log CFU/mL and decreased with storage. The lactic acid bacteria count of the K group was initially 4.80 log CFU/mL and increased until month 5 to approximately 6.06 log CFU/mL. The K group scored higher in terms of sensory attributes than the other groups and maintained marketable scores for all relevant properties (color, flavor, off-odor, and overall acceptance). Furthermore, the free amino acid content of the K group was the highest among all groups at approximately 3,000 mg per 100 g. These results suggest that K treatment may be beneficial in the preparation of fermented fish sauce.
Quality of Alaska Pollack Theragra chalcogramma Sikhae after Fermentation for Different Times
Jeong, Eun-Jeong ; Kim, Hun ; Cha, Yong-Jun ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 3, 2015, Pages 293~300
DOI : 10.5657/KFAS.2015.0293
We sought to extend the shelf-life of Alaska pollack Theragra chalcogramma sikhae while maintaining quality. We compared the chemical, microbiological, and organoleptic characteristics of sikhae prepared under four different conditions. Control fish were fermented at ambient temperature (
); other samples were stored at
after fermentation at ambient temperature for 36 h (A1), 60 h (A2), and 84 h (A3). Volatile basic nitrogen and amino-nitrogen levels, and total acidity increased with fermentation time in all samples, but the pH fell, attaining a relatively lower level in the control than in other samples. Over 90% of all viable cells were lactic acid-producing bacteria; this proportion did not change significantly during fermentation. In terms of texture, only hardness was affected by fermentation. The hardness of the control fell more rapidly than did that of the other samples. In terms of sensory evaluation (the acceptance test and quantitative descriptive analysis [QDA]), A2 was superior to other samples after fermentation for different times; A2 maintained limited salability (6 points on the relevant index) for up to 17 days of storage.
Isolation of Eckol from Ecklonia cava via Centrifugal Partition Chromatography (CPC) and Characterization of it's Anti-inflammatory Activity
Kim, Yoon Taek ; Lee, Ji-Hyeok ; Ko, Ju-Young ; Oh, Jae-Young ; Lee, Won-U ; Sok, Chang Hyun ; Hong, Jin Tae ; Jeon, You-Jin ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 3, 2015, Pages 301~307
DOI : 10.5657/KFAS.2015.0301
Phlorotannins and marine algal polyphenols, including dieckol, 6,6-bieckol, phloroglucinol, phlorofucofuroeckol-A, and eckol, were isolated from brown seaweeds. These compounds have beneficial bioactivities, and Ecklonia cava has become widely used for the extraction and isolation of phlorotannins. Eckol, in particular, has been to shown to have antioxidant, anti-inflammatory, anticoagulatory, and photoprotective properties. However, due to its low abundance in weaweed, the isolation and purification of eckol are difficult. Its limited availability renders the isolation and purification of eckol labor-intensive processes. Centrifugal partition chromatography (CPC) is an efficient technique for the isolation and purification of eckol. In this study, eckol was isolated from the ethyl acetate fraction of the 70% ethanol extract of E. cava using CPC with a two-phase solvent system of a n-hexane:EtOAc:methanol:water (2:8:3:7, v/v) solution. The purity and anti-inflammatory activity of the isolated eckol were verified by high-performance liquid chromatography and by assaying lipopolysaccharide-induced inflammatory responses in an immortalized murine BV2 microglial cell line, respectively. In conclusion, CPC is a useful technique for simple and efficient isolation of eckol from E. cava.
Effects of Dietary Protein and Lipid Levels on the Growth Performance, Feed Utilization and Innate Immunity of Juvenile Red Seabream Pagrus major
Kim, Sung-Sam ; Oh, Dae-Han ; Choi, Se-Min ; Kim, Kang-Woong ; Kim, Kyoung-Duck ; Lee, Bong-Joo ; Han, Hyon-Sob ; Lee, Kyeong-Jun ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 3, 2015, Pages 308~313
DOI : 10.5657/KFAS.2015.0308
factorial study was conducted to investigate the effects of dietary protein and lipid levels on the growth, feed utilization and innate immunity of red seabream Pagrus major. Nine diets consisting of three protein levels (42%, 46% and 50% crude protein) and three lipid levels (10%, 14% and 18% crude lipid) were formulated. Triplicate groups of red seabream were fed the experimental diets to apparent satiation (5-6 times a day, from 08:00 to 18:00 h at 2-h intervals) for 10 weeks. At the end of the feeding trial, the weight gain and specific growth rate of fish fed P46L14 (46% protein and 14% lipid), P50L10 (50% protein and 10% lipid) and P50L14 (50% protein and 14% lipid) were significantly (P<0.05) higher than those of fish fed P42L18 (42% protein and 18% lipid). The feed conversion ratios (FCR) of the fish were affected by dietary lipid levels (P<0.039), but not dietary protein levels. The FCR tended to increase with increasing dietary lipid levels from 10% to 18% with the 46% and 50% protein levels. The weight gain, protein efficiency ratio, specific growth rate, feed intake and survival of fish were not affected by either dietary protein or lipid levels. Myeloperoxidase activity in the group fed P50L14 (50% protein and 14% lipid) was significantly higher than that in the group fed P42L10 (42% protein and 10% lipid) or P50L18 (50% protein and 18% lipid). However, the myeloperoxidase activity of fish was not affected by either dietary protein or lipid level. The fish fed P46L14 (46% protein and 14% lipid) and P46L18 (46% protein and 18% lipid) showed significantly higher superoxide dismutase activity than did the fish fed P46L10 (46% protein and 10% lipid), P50L10 (50% protein and 10% lipid) of P50L18 (50% protein and 18% lipid). In conclusion, the optimum protein and lipid levels for the growth and feed utilization of juvenile red seabream were 46% and 14%, respectively, and the optimum dietary protein to energy ratio was 27.4 g/MJ.
Effects of Feed Particle Size, Stocking Density, and Dissolved Oxygen Concentration on the Growth of Olive Flounder Paralichthys olivaceus
Kim, Sung-Sam ; Lee, Jin-Hyuck ; Kim, Kang-Woong ; Kim, Kyoung-Duck ; Lee, Bong-Joo ; Lee, Kyeong-Jun ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 3, 2015, Pages 314~321
DOI : 10.5657/KFAS.2015.0314
We performed three sets of feeding trials to establish the optimal feed size (Exp-I), stocking density (Exp-II), and dissolved oxygen level (DO) (Exp-III) for olive flounder Paralichthys olivaceus. In Exp-1, four replicate groups of fish (
) were fed commercial diets with three particle sizes (small, medium, and large). In Exp-II, fish (
) were reared at four stocking densities (1.8, 3.5, 5.3, and
). In Exp-III, fish (
) were reared under two different DO levels (2-3 and 6-7 mg/L). In Exp-I, fish fed the large-particle diet gained significantly more weight and had a lower feed conversion ratio than fish fed the small- and medium-particle diets. In Exp-II, fish reared at 1.8 and
gained slightly more weight and had lower feed conversion ratios than fish reared at 5.3 and
, although these differences were not significant. In Exp-III, negative effects were observed in the low DO groups. Therefore, under our experimental conditions, the optimal feed particle size, stocking density, and DO level for olive flounder were 9-9.4 mm,
, and 6-7 mg/L, respectively.
Optimal Feeding Rates of Subadult Korean Rockfish Sebastes schlegeli Fed a Commercial Diet at Different Water Temperatures
Kim, Min-Gi ; Lee, Cho-Rong ; Kim, You-Jeong ; Oh, Dae-Han ; Lee, Bong-Joo ; Kim, Sung-Sam ; Lee, Kyeong-Jun ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 3, 2015, Pages 322~328
DOI : 10.5657/KFAS.2015.0322
Three feeding trials were conducted to investigate the optimal feeding rates of subadult Korean rockfish Sebastes schlegeli at low, optimal and high water temperatures. In Exp-I, triplicate groups of fish (248 g) were fed a commercial diet (CD) with five different feeding rates of 0.1%, 0.2%, 0.4%, 0.6% and 0.8% body weight per day for 10 weeks at the low temperature (
). In Exp-II, fish (129 g) were fed the same CD with five different feeding rates of 0.4%, 0.8%, 1.2%, 1.6% and 2.0% body weight per day for 10 weeks at the optimal temperature (
). In Exp-III, fish (85.7 g) were fed the same CD with five different feeding rates of 0.4%, 0.8%, 1.2%, 1.6% and 2.0% body weight per day for 12 weeks at the high temperature (
). The optimal feeding rates in the low, optimal, and high water temperatures were estimated to be 0.56%, 0.93% and 1.17% body weight per day, respectively, for subadult Korean rockfish.
The Effects of Substituting Squid Meal and Macroalgae with Soybean Meal in a Commercial Diet on Growth and Body Composition of Juvenile Abalone Haliotis discus hannai
Kim, Byeng-Hak ; Kim, Hee Sung ; Cho, Sung Hwoan ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 3, 2015, Pages 329~336
DOI : 10.5657/KFAS.2015.0329
We examined the effect of substituting squid meal and macroalgae with soybean meal in a commercial diet on the growth and body composition of juvenile abalone Haliotis discus hannai. We randomly distributed 2310 juvenile abalone into 33 rectangular plastic containers and fed them five experimental diets in triplicate as follows. The control diet (Con) consisted of 12% squid meal, 8% corn gluten and 20% soybean meal as protein source, wherein 10%
-starch, 20% wheat flour, and 5% dextrin were carbohydrate source. The experimental diets, 50% squid meal (SM50), 50% squid meal and 50% macroalgae (SM50+MA50), and 100% squid meal and 50% macroalgae (SM100+MA50) were substituted with the same respective amounts of soybean meal. The fifth experimental diet consisted of the control diet plus 1% diatom powder (DP). We prepared two domestic (Domestic A and B) and two imported (China and Japan) abalone feeds. Finally, we prepared Undaria and sea tangle. We found that the weight gain of abalone fed the Con, DP, and China and Japan diets was significantly greater than that of abalone fed Undaria and sea tangle. We conclude that the substituting squid meal and macroalgae with soybean meal in abalone feed has limited benefits, but supplementing diets with 1% diatom powder is effective in improving weight gain.
Characterization of the Myxosporean Parasite Isolated from Emaciated Olive Flounders Paralichthys olivaceus on Jeju Island
Kim, Seung Min ; Jun, Lyu Jin ; Park, Myoung Ae ; Jeong, Hyun Do ; Jeong, Joon Bum ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 3, 2015, Pages 337~345
DOI : 10.5657/KFAS.2015.0337
To investigate the causes of emaciation in cultured olive flounder Paralichthys olivaceus in Korea. We performed histological examinations and polymerase chain reaction (PCR) with a new primer set. In most cases, the most severe emaciation was observed in the abdominal area Using PCR on extracted livers, kidneys, spleens, gills, brains, and intestines, we found that areas around the kidneys and intestines were as almost always positive. In significantly emaciated fish, PCR was positive in all internal organs except the gills. In addition, the homology of 812-bp nucleotide sequences of the 28S rRNA gene was more than 99% in emaciated fish. Partial homology with Myxobolus spp. and Cystodiscus axonis, whose data were obtained from GenBank was 86% and 88%, respectively. Histological examinations detected spores in kidneys and intestines but not in other organs. We also performed cohabitation experiments to determine whether infections could be exchanged among species or only within species. Uninfected olive flounder and red sea bream, Pagrus major, cohabitating with emaciated olive flounder showed 100% and 0% cumulative mortality, respectively. Thus the cause of emaciation in cultured olive flounder of Korea is likely due to a new parasite.
Molecular Genetic Characterization and Analysis of Glucocorticoid Receptor Expression in the Big-belly Seahorse Hippocampus abdominalis
Jo, Eunyoung ; Oh, Minyoung ; Lee, Sukkung ; Qiang, Wan ; Lee, Jehee ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 3, 2015, Pages 346~353
DOI : 10.5657/KFAS.2015.0346
Glucocorticoids (GCs) are steroid hormones regulated through responses to stress to maintain diverse metabolic and homeostatic functions. GCs act on the glucocorticoid receptor (GR), a member of the nuclear receptor family. This study identified and characterized the GR gene from the big-belly seahorse Hippocampus abdominalis designating it HaGR. The open reading frame of the HaGR cDNA was 2,346 bp in length, encoding a 782-amino-acid polypeptide with a theoretical isoelectric point of 6.26 and predicted molecular mass of 86.8 kDa. Nuclear receptors share a common structural organization, comprising an N-terminal transactivation domain, DNA-binding domain, and C-terminal ligand-binding domain. The tissue-specific mRNA expression profile of HaGR was analyzed in healthy seahorses using a qPCR technique. HaGR mRNA was expressed ubiquitously in all of the tissues examined, with the highest expression levels in kidney, intestine, stomach, and gill tissues. The mRNA expression in response to immune challenge with lipopolysaccharide (LPS), polyinosinic:polycytidylic acid (poly I:C), Edwardsiella tarda, and Streptococcus iniae revealed that it is inducible in response to pathogen infection. These results suggest that HaGR is involved in the immune response of the big-belly seahorse.
Characterization of Mitochondrial Heat Shock Protein 75 (mtHSP75) of the Big-belly Seahorse Hippocampus abdominalis
Ko, Jiyeon ; Qiang, Wan ; Lee, Sukkyoung ; Bathige, S.D.N.K. ; Oh, Minyoung ; Lee, Jehee ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 3, 2015, Pages 354~361
DOI : 10.5657/KFAS.2015.0354
Mitochondrial heat shock protein 75 (mtHSP75) is a member of the HSP90 family and plays essential roles in refolding proteins of the mitochondrial matrix. Mitochondria provide energy in the form of ATP and generate reactive oxygen species (ROS). Heat shock proteins (HSPs) are activated in response to stress, and protect cells. In this study, we characterized the mtHSP75 of the big-belly seahorse Hippocampus abdominalis. The protein (BsmtHSP75) is encoded by an open reading frame (ORF) of 2,157 nucleotides, has 719 amino acids (aa), and is of molecular mass 82 kDa. BsmtHSP75 has two functional domains, a histidine kinase-like ATPase (HATPase_c) domain (123-276 aa) and an HSP90 family domain (302-718 aa). BsmtHSP75 was expressed in all tested tissues of healthy seahorses. The ovary contained the highest transcription level, followed (in order) by the blood, brain, and muscle. Pouch tissue showed the lowest expression level. The expression of BsmtHSP75 was significantly (P<0.05) up-regulated on viral or bacterial challenge, suggesting that BsmtHSP75 plays a role in the immune defense against bacterial and viral pathogens.
Development of Naturally-spawned Pacific Herring Clupea pallasii Larvae
Ji, Hwan-Sung ; Lee, Dong Woo ; Choi, Jung Hwa ; Choi, Kwang Ho ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 3, 2015, Pages 362~367
DOI : 10.5657/KFAS.2015.0362
We followed the development of Pacific herring Clupea pallasii larvae after natural hatching in Korean coastal waters off Dadaepo, where the water temperature was
. Twenty days after hatching, the larvae had (i) reached a total length (TL) of 10.8-12.2 mm, (ii) developed 9-11 dorsal fin rays, and (iii) branched melanophores along the dorsal line of the gut in the anterior half of the body and along the posterior half of the dorsal and ventral line. Thirty days after hatching, the larvae had reached 12.2-13.5 mm TL, and the number of dorsal fin rays had increased to 13-14. Thirty-five days days after hatching, the larvae had reached 14.0-14.7 mm TL, and the posterior ends of their notochords had begun to flex upward. Forty-five days days after hatching, the larvae had (i) reached 15.6-15.9 mm TL, (ii) a complete set of dorsal fin rays (15-16), (iii) 12-13 anal fin rays, and (iv) branched melanophores along the dorsal part of the lateral surfaces of the head behind the caudal terminus. Preflexion, flexion and postflexion stage larvae had TL values of 13.5 mm, 14.0-15.3 mm, and 15.6-15.9 mm, respectively.
Feeding Habits of Larval Liparis tanakae from the Nakdong River Estuary, Korea
Choi, Hee Chan ; Huh, Sung Hoi ; Park, Joo Myun ; Baeck, Gun Wook ; Suh, Young Sang ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 3, 2015, Pages 368~376
DOI : 10.5657/KFAS.2015.0368
The Feeding habits of larval Liparis tanakae collected from the Nakdong River estuary were studied. L. tanakae [3.8-10.6 mm notochord length(NL)] was a carnivore thatconsumed mainly copepods. Its diet also included small numbers of free-living flatworms (Macrostomida), tintinnids, cladocerans, and polychaetes larvae. To evaluate ontogenetic changes in dietary composition, three size groups were designated: 3-5, 5-7, and >7 mm. Copepods were the most important prey items for all size groups. The smallest size group frequently preyed on polychaete and bivalve larvae, along with copepods. As notochord length increased, the numbers of cladocerans and Macrostomida increased relative to those of polychaete and bivalve larvae.
Age and Growth of the Skipjack Tuna Katsuwonus pelamis in the Western and Central Pacific Ocean
Ku, Jeong Eun ; Lee, Sung Il ; Kim, Jin-Koo ; Park, Hee Won ; Lee, Mi Kyung ; Kim, Zang Geun ; Lee, Dong Woo ;
Korean Journal of Fisheries and Aquatic Sciences, volume 48, issue 3, 2015, Pages 377~385
DOI : 10.5657/KFAS.2015.0377
The age and growth of the skipjack tuna Katsuwonus pelamis were determined using otoliths sampled from a Korean tuna purse seine fishery in the Western and Central Pacific Ocean from January 2005 to September 2006. A total of 312 otoliths were used to estimate the ages of skipjack tuna, which ranged from 1 to 7 years. The relationships between otolith ring radius (R) and fork length (FL) for female, male, and sex combined were FL = 19.74R + 1.50 (
), FL = 17.66R + 6.35 (
), and FL = 18.83R + 3.36 (
), respectively. The back-calculated fork lengths of each age (
. The relationships between fork length (FL) and total weight (TW) for female, male, and sex combined were
, respectively. The von Bertalanffy growth parameters of skipjack tuna estimated in this study were
, K = 0.176/year, and