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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 15, Issue 4 - Dec 1977
Volume 15, Issue 3 - Sep 1977
Volume 15, Issue 2 - Jun 1977
Volume 15, Issue 1 - Jan 1977
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Optimization of Penicillin Amidase Production and A Simplified Enzyme Assay Method
The Korean Journal of Microbiology, volume 15, issue 4, 1977, Pages 145~153
Penicillin amidase (EC 18.104.22.168) was produced by a mutant strain of Bacillius megaterium ATCC 14945. Hydroxylamine assay method for the determination of 6-APT was modified by using "HCl addition techniques" in order to simplify the time consuming orginal assay method without sacrifice of accuracy. Using the new mutant strain, the effects of fermentation conditions on enzyme production were studied.e studied.
Radiosterilization of Medical Products(III)
The Korean Journal of Microbiology, volume 15, issue 4, 1977, Pages 154~158
Fifteen kinds of antibiotics both in liquid or dry stantes wre investigated for the purpose of radiation effect on the antibiotic activities after irradiation. In liquid states, the antibiotics such as amoxyllin, ampicillin, capharolidin and hetacillin were inctivated 90% of their antibiotic activities at radiation doses of 50Krad, and penicillin, dicloxacillin, caphalothin, cloxacillin, erythromycin and caphalexin and kanamycin were decomposed at radiation doses of 1.5Mrad, respectively. In dry states, all antibiotics were stable at radiation doses of 5Mrad. On the other hand, the neomycin in liquid state was rather increased the antiboctic activity about 1.2 and 8 times when the E. coli ATCC 113-3 and B. subtitilis ATCC 6633 were used aas a standard microorganisms, whereas, the decrensed antibiotic activities were observed in S. typhi Ty-2(36.2%), St.faecium(10.79%), B.sphaericus CIA(9.066%) and St.aureus ATCC 6538p(70%).
STudies on the Microbial Pigment(I)
Ahn, Tae-Seok ; Choi, Yong-Keel ; Hong, Soon-Woo ;
The Korean Journal of Microbiology, volume 15, issue 4, 1977, Pages 159~169
The bacteria of red colonies isolated from soil were identified as Serratia marcescens. The best solvent for pigment extraction was n-buthanol and the pigment was identified as prodigiosene. The extracted pigment was stable on temperature and light but not on acidity. The redpigment color changed into red in alkaline solution. The maximum absorbancy of pigment was 466 nm in alkaline condition and 540 nm in acid condition. And the pigment formed single spot on the TLC(starch). By the result of infra red spectrum, the red pigment has the same absorption pattern comparing with, the prodigisin produced by S. marcescens strain Nima. It was confirmed that the pigment was secondary metabolite and that the maximal peak of production appeared at 30 hrs after the inoculation, when the bacterial growth was in statinary state. Referring to the effect of temperature, the pigment was not formed at
and the optimal temperature for both of bactrial growth and pigmentation was
. The optimal range of pH for pigmentation was 5.0 and under the condition the bacterial growth was not affected at all. Examining the effects of light, the bacterial pigment ation was more increased in darkness than in visible light.
The Isolation and Identification of Some Streptomyces
The Korean Journal of Microbiology, volume 15, issue 4, 1977, Pages 170~175
A taxonomical study was made on the Streptomyces species isolated from soils collected in Heoju and Mt. Jiri, from May to June, 1977. I.S.P. Methods (1966) and I.S.P.Descriptions(1968-1972) were used for the methods and identifications. As a result, 6 species were newly identified as follows ; S.tanashiensis, A.virido-didastaticus, S.rutgersensis, S. citreus, A.alborubidus and S.fragils.