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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 16, Issue 4 - Dec 1978
Volume 16, Issue 3 - Sep 1978
Volume 16, Issue 2 - Jun 1978
Volume 16, Issue 1 - Mar 1978
Selecting the target year
Characterization of D-Xylose Isomerase from Streptomyces albus
The Korean Journal of Microbiology, volume 16, issue 2, 1978, Pages 47~61
Strptomyces albus T-12 which ahd been isolated and identified in the laboratory, was selected for the studies on the cultural conditions on the production of D-xylose iosmerase and the enzymological characteristics using the partially purified enzyme. The best results in the enzyme production came from D-xylose medium than wheat bran. The divalent metla ions as
retard or inhibit the cell-growth at the early stages of mycelia propagations, and T-12 strain is especially sensitive to
. After 60 hours of shaking cultivation at
and 200 rpm, a maximum enzyme activitz, 0.49 enzyme units, was obtained. Cell-free enzyme obtained from mycelia heat-treated in the prescence of 0.5mM
, showed a 2.4-fold increase in specific than the enzyme from untreated mycelia. The specific activity of the purified enzyme through Sephadex G-150 columm showed 180 fold to the crude enzyme. The effective activators of the enzyme appeared to be
ions, and it exhibited the maximal enzyme activity showed at pH 7.0 and at tempersture around
ions were added. The enzyme isomerized D-glucose, D-xylose, D-ribose, L-arabinose, D-mannose, and L-rhamnose in the present of
ions as an activatiors.
ions were non-competitively bound at different allosterix sites of enzyme molecule.
protected against the thermal denaturations of the enzyme activities. The michelis constant(Km) and
values of the emzyme for D-glucose and D-xylose were 0.52M,
Inactivation of Bacteriophage f2 with Chlorine
Chi Kyung KIM ; Kyung Hee MIN ;
The Korean Journal of Microbiology, volume 16, issue 2, 1978, Pages 62~70
Chlorine was used for inactivation of bacteriophage f2 at pH 5.5, 7.5, and 10.0 at
. The inactivation rate phage with chlorine varied depending on the pH value and reaction time. Hypochlorous acid appeared to be the major species of free chlorine for the inactivation. Suevival of the phage treated with chlorine and infectivity of the RNA extracted from the chlorinated phage were examined. The RNA extracted from untreatd phage was chlorinated and its infectivity was assayed. All three samples showed similar rates of inactivation at pH 5.5 and 7.5, but the naked RNA was more susceptible to chlorine at pH 10.0. The rate of inactivation was compared naked RNA was more susceptible to chlorine at pH 10.0. The rate of inactivation was compared with specific and non-specific attachment of the phasge f2. The specific attachment of the phage increased after the phage had been inactivated by extended chlorination. Chlorine may penetrate to the becteriophage f2 by altering the structural integrity of the protein coat, but the main target of free chlorine for inactivation of the phage appeared to be the phage RNA.
Studies on the Isolation and Characterization of Bacteriophage of Bacillus subilis var, S16
The Korean Journal of Microbiology, volume 16, issue 2, 1978, Pages 71~78
Bacillus subtilis var. 816 was used for manufacturing fermented soybean which in turn is used as flavoring agent. Fermentation of soyebean or flat wheat was occasionally failed. It was elucidated that failure was due to the presence of bacteriophage. According to Hemphill and Whitely (1975), this bacteriphage might be belonged to the viulent phage group I as it is similar to SP82G,
. In fact, the phenomena of the increase of moisture, disappenarance of mucin and existence of undersirable bacteria was attributed to the contamination of the above phage during the course of fermentation of soybean or flat wheat. Particularly disapparance of mucin was sufficiently correlated by the replication of the bacteriophage. The above phage can grow in the range of
. The optimum temperature was
. The optimum pH range was between pH 7.4 and pH 8.0. It is noticeable that staphylococci was replicating simultaneously with the phage. The head of 816 phage is hexagonal with a diameter of
. The end of the tail is enlaged. It has a size of 25 nm and this end areas are spreaded widely as fingers.
Degradation of Benzenoids by Microorganisms
The Korean Journal of Microbiology, volume 16, issue 2, 1978, Pages 79~89
The RK-temperate phage which infected with Bacillus cereus was isolated and the characters were investigated. The induction of RK-temperate phage from host bacterium attained by ultraviolet light irradiation (15W, 30cm, 30-120sec) and mitomycin C treatment (0.2-2 ug/ml). The host range of RK-temperate phage was not revealed with lysogenic and related strains of B. cereus. But B. cereus(PS) 352 which obtained by N-nitrosoguanidine treatment(1,000.
g/ml) to phage infected with host bacteria was sensitive bacteria of RK-temperate phage. RK-temperate phage was stabilized at the condition of nutrient broth (pH 7-8), Tris-buffer (pH 7-8) and ammonium buffer (pH 8-9) and Sorensen's phosphate buffer (pH 6-7), but unstabilized at other salt solutions and pH range. Also, thermostability was to 45.deg.C but unstabilized at above 50.deg.C. At RK-temperate phage, the measurment values of head, neck, mid tail and end tail were 59nm, 9*16nm, 10*189nm, and 10*14nm respectively. The morphology of head was regular polyhedron, and the end tail was coneate form. On the one hand, the number of capsid protein layer of tail were consist of 4, 35, and 1 at neck, mid tail, and end tail, respectively. RK-temperate phage was identified with DNA phage and G+C contents were 38.63. The latent time of RK-temperate phage was 30 minutes and the burst size was 70-80. And the host bacteria was lysed in case of multi-infection, above moi 1.