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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 17, Issue 4 - Dec 1979
Volume 17, Issue 3 - Sep 1979
Volume 17, Issue 2 - Jun 1979
Volume 17, Issue 1 - Mar 1979
Selecting the target year
Classification and Characterization of Bacteriophages of Lectobacillus casei
The Korean Journal of Microbiology, volume 17, issue 4, 1979, Pages 165~178
Phages of Lactobaciilus casei (PLC) isolated from plant drainage were classified and characterized. The results are as follows : 1. On the basis of host range pattern, phages could be divided into 2 groups (PLC-B and PLC-C). PLC-B group phages could be further divided into 5 sub-groups
. Although PLC-C group phages had the same host range, they could be also divided into 2 sub-groups
by morphlogical type. 2. It was
group phages that represented a major proportion (44.4%) of phages tested. However,
group phages were shown to have the widest host range. 3. Electron micrographs revealed that the phages fell into three different morphological types.
group phages hd a hexagonal head (52nm in diameter) and a sheathless noncontractile (245 nm in length).
group phages had a hexagonal head (56 nm) and a short flexible tail (169nm) having no sheath.
group phages were shown to have a hexagonal head (81 nm) and a contractile tail (140 nm) having a sheath, a base plate and tail fibers. 4. The inactivation of the phages by antisera indicated that serological relationships correlated completely with morphological types. 5.
group phages produced a large (1, 2 mm in diameter) plaque with a clear ring. The morphology of plaques of
group phages was the same as those produced by the above, but the average plaque sizes for
were 0.8 mm abd 0.5 mm, respectively.
group phages produced a small (0.5 mm) turbid plaque with an irregular edge. 6. The latent period and the average burst size of
group phages were 90 min and 100, respectively. These phages reuqired calcium ions for their miltiplication. 7.
group phages could not be absrobed to R-variant
. 8. The order of resistance of phages to heat was $B_2\;>\;B_1, B_4\;and\;B_5\;>\;B_3\;and\;C_2, \;B_5$ group phages were more stable than
in various pH values.
group phages were more sensitive to UV irradiation than
group phages. 9. Strains YIT9018 and IAM 1043 were induced by mitomycin C treatment. Phage particles detected in the lysates had a hexagonal head (38 and 49 nm, respectively), but no tail. Any sensitive indicator strain could not be isolated in spite of repaeated trials.
Studies on the Etiology of Red Rot of Ginseng
The Korean Journal of Microbiology, volume 17, issue 4, 1979, Pages 179~186
Rotting bacteria were isolated from decayed root rot of ginseng (Panax ginseng C.A. Meyer), cultured purely, and it's pathogenicity was confirmed by reinoculation test. Two strains (E3, E7) were selected to be tested in more detailed study with respect to their morphological, cutural and biochemical characters. The strains causing red rot of ginsneg were identified as Erwinia carotovora.
On the CMCase Activity from Two species of Trichosporon
The Korean Journal of Microbiology, volume 17, issue 4, 1979, Pages 187~192
Dennis (1972) reported that Trichosporon cutaneum FRI-425 from the petioles of Pheum rhamponticum var, had showed the celluloytic activity. Chun (1977) also suggested that Trichoporon pullulons 225 isolated from the saline water of the Yeoung San River had a similar properties. However, the assay conditions for enzyme activity were not yet investigated. Thus, the present work was undertaken to examine some conditions for CMCase activity and at the same time to compare the activities of crude enzyme produce from above two species of Trichosporon pullulans. The results are as follows; 1. The maximum production of total reducing sugar by crude enzyme of Tr. pululans was after 30 minutes, whereas that of Tr. cutanuem FRI-425 was after 90 minutes. This fact showed that the reaction velocity of enzyme from Tr. pullulans 225 was more faster than that of Tr. cutaneum FRI-425. 2. Two species showed a similar trend to increase the production of reducing sugar in proportion to the increment in substrate concentration and to arrive at maximum level at lmg/ml of substrate concentration. However, Tr. pullulans 225 produced more
of reducing sugar compared to Tr. cutaneum. 3. The optimum PH for CMCase activity is 5.0 for Tr. pullulans 225 as well as Tr. cutaneum FRI-425, and PH stability lie within the range of 6 and 8. In the activity and stability of enzyme on PH changes, enzyme of Tr. cutaneum FRI-425 was more unstable than that of TY. pullulans 225. 4. The optimum temperature for CMCase activity was
, and enzyme activity from Tr. pullulans 225 was more sensitive to temperature changes compared with that of TY. cutaneum. The heat stability was within
, but that was rapidly decreased above
. In comparison of the heat stability for enzyme of Tr. cutaneum FRI-425 with that of Tr. pullulans 225 at the same temperature of
, the former was some 10 percent more stable than the latter.
Bioconversion of Furfural into 2-Furoic acid by Zooglea sp.
The Korean Journal of Microbiology, volume 17, issue 4, 1979, Pages 193~197
Attempts were made to elucidate the process of biodegradation of furfural. Zoogloea sp. isolated from soil could convert furfural into 2-furoic acid by a certain enzyme (s) and also accumulated it extracellularly. This substance was extracted with diethyl ether and identified with U.V. spectrophotometry, high pressure liquid chromatography (HPLC) and IR spectrophotometry.
Symbiotic Biodegradation of Furfural by Some Bacteria
The Korean Journal of Microbiology, volume 17, issue 4, 1979, Pages 198~202
Three Pseudomonas spp. and one Zoogloea sp. which could docompose the furfural were isolated from the enriched undefined cultures of soil. In the decompositioin of furfural thyey demonstrated protocooperation and synergism, utilizing 2-furoic acid a certain sudstance fural was subject to complete oxidation, which resulted in decolorization by mutural interactions. The decomposition was more efficient in mixed cultures than in a single culture.